http://sedici.unlp.edu.ar:80/handle/10915/223 2013-05-20T08:54:01Z 2013-05-20T08:54:01Z Santos Oliveira, Ralph http://sedici.unlp.edu.ar:80/handle/10915/7815 2012-05-03T01:50:47Z 2009-01-01T00:00:00Z

Comunicacion Validation of PTS for radiopharmaceuticals endotoxin test Latin American Journal of Pharmacy; vol. 28, no. 4 O presente estudo foi realizado visando a validação do PTS_TM (Portable Test System) como metodologia padrão para ensaio de endotoxina em radiofármacos (18-FDG). A análise foi guiada pela Resolução Anvisa RE 899/2003. Os resultados demonstram adequação da técnica à rotina de radiofármacos, mesmo que no quesito exatidão os resultados tenham sido desfavoráveis a sua utilização. Contudo, avaliando-se o benefício de seu uso, a técnica mostrou-se uma alternativa ao teste farmacopéico atual.; The endotoxin test is an important parameter for medicines, specially the radiopharmaceuticals one, since the presence of endotoxin can be dangerous. Nowadays, two techniques are been used for radiopharmaceuticals endotoxin test. First, the traditional one, the BET (Bacterial Endotoxin Test) described in United States Pharmacopoeia. Second the PTSTM (Portable Test System) approved by the FDA. However, since the PTS_TM is not a pharmacopeial test he must be validated before been used for routine. The present study was conducted to validated the PTS_TM as standard methodology for endotoxin assay. The results, except accuracy, were good. Although the results of accuracy were not good this technique seems to be a important alternative test, especially for radiopharmaceuticals.

2009-01-01T00:00:00Z O presente estudo foi realizado visando a validação do PTS_TM (Portable Test System) como metodologia padrão para ensaio de endotoxina em radiofármacos (18-FDG). A análise foi guiada pela Resolução Anvisa RE 899/2003. Os resultados demonstram adequação da técnica à rotina de radiofármacos, mesmo que no quesito exatidão os resultados tenham sido desfavoráveis a sua utilização. Contudo, avaliando-se o benefício de seu uso, a técnica mostrou-se uma alternativa ao teste farmacopéico atual. The endotoxin test is an important parameter for medicines, specially the radiopharmaceuticals one, since the presence of endotoxin can be dangerous. Nowadays, two techniques are been used for radiopharmaceuticals endotoxin test. First, the traditional one, the BET (Bacterial Endotoxin Test) described in United States Pharmacopoeia. Second the PTSTM (Portable Test System) approved by the FDA. However, since the PTS_TM is not a pharmacopeial test he must be validated before been used for routine. The present study was conducted to validated the PTS_TM as standard methodology for endotoxin assay. The results, except accuracy, were good. Although the results of accuracy were not good this technique seems to be a important alternative test, especially for radiopharmaceuticals. López H., Orestes Darío Márquez Conde, Tania Mayo A., Orestes Toledo S., Carlos Pérez S., Enma http://sedici.unlp.edu.ar:80/handle/10915/7814 2012-05-03T01:50:47Z 2009-01-01T00:00:00Z

Comunicacion Characteristics of microencapsulated Cucurbita pepo L. seeds oil formed by spray drying with maltodextrin and arabic gum Latin American Journal of Pharmacy; vol. 28, no. 4 Para elaborar una forma sólida de administración oral a partir del extracto lipídico de las semillas de Cucurbita pepo L. usado en el tratamiento de la hiperplasia benigna prostática, la alternativa de la microencapsulación ofrece una solución para modificar su estado físico. Las microcápsulas obtenidas mediante secado por aspersión, empleando maltodextrina y goma arábiga como encapsulantes, alcanzándose más de un 80% en cuanto a eficiencia de encapsulación. Se verificó la forma esférica y la ausencia de poros en la superficie de las microcápsulas mediante microscopía de barrido electrónico. Se realizó la determinación de la distribución del tamaño de partículas, siendo el diámetro medio de 34,28 μm. También se determinó el contenido de ácidos grasos y la pérdida por desecación, demostrándose la reproducibilidad de los resultados obtenidos a escalas de laboratorio y banco.; To elaborate an oral solid dosage form from Cucurbita pepo L. seeds from lipidic extract used in the treatment of benign prostatic hyperplasia, microencapsulation offers a solution to modify its physical conditions. Microcapsules obtained by spray drying using maltodextrin and arabig gum as encapsulant agent reached over 80% of encapsulation efficiency. The spherical form and the absence of pores on the surface of the microcapsules were verified by scanning electron microscopy. The particle size distribution was 34,28 μm of average diameter. The content of fatty acids and loss on drying was also determined, which proved the reproducibility on the results in laboratory and bank scales.

2009-01-01T00:00:00Z Para elaborar una forma sólida de administración oral a partir del extracto lipídico de las semillas de Cucurbita pepo L. usado en el tratamiento de la hiperplasia benigna prostática, la alternativa de la microencapsulación ofrece una solución para modificar su estado físico. Las microcápsulas obtenidas mediante secado por aspersión, empleando maltodextrina y goma arábiga como encapsulantes, alcanzándose más de un 80% en cuanto a eficiencia de encapsulación. Se verificó la forma esférica y la ausencia de poros en la superficie de las microcápsulas mediante microscopía de barrido electrónico. Se realizó la determinación de la distribución del tamaño de partículas, siendo el diámetro medio de 34,28 μm. También se determinó el contenido de ácidos grasos y la pérdida por desecación, demostrándose la reproducibilidad de los resultados obtenidos a escalas de laboratorio y banco. To elaborate an oral solid dosage form from Cucurbita pepo L. seeds from lipidic extract used in the treatment of benign prostatic hyperplasia, microencapsulation offers a solution to modify its physical conditions. Microcapsules obtained by spray drying using maltodextrin and arabig gum as encapsulant agent reached over 80% of encapsulation efficiency. The spherical form and the absence of pores on the surface of the microcapsules were verified by scanning electron microscopy. The particle size distribution was 34,28 μm of average diameter. The content of fatty acids and loss on drying was also determined, which proved the reproducibility on the results in laboratory and bank scales. Kamila, Madan M. Mondal, Nita Gupta, Bijan Kumar Ghosh, Lakshmi K. http://sedici.unlp.edu.ar:80/handle/10915/7813 2012-05-03T01:50:47Z 2009-01-01T00:00:00Z

Comunicacion Latin American Journal of Pharmacy; vol. 28, no. 4 The aim of the present study was to prepare, characterize and in-vitro evaluation of a Winsor-IV type microemulsion based drug delivery system incorporating celecoxib as BCS class-II model drug. Attempts were made to prepare cost effective O/W microemulsion using Tween 80, Glycerol, sunflower oil and water. The existence of microemulsion zone was investigated using phase diagrams. The systems were characterized by polarized light microscopy, viscosity, refractive index, droplet size of dispersed phase by dynamic light scattering technique, thermal and centrifugal stability and the drug release profile. The obtained microemulsion was found optically isotropic with non-Newtonian behavior. The average droplet size was 100-300 nm. Microemulsions showed reversibility of transparency at ambient temperature after storage at 5 °C. The solubility enhancement of formulated products was apparent from higher release rate from microemulsion as compared to commercial product. The drug release profile was demonstrated to be promising for oral delivery of celecoxib.

2009-01-01T00:00:00Z The aim of the present study was to prepare, characterize and in-vitro evaluation of a Winsor-IV type microemulsion based drug delivery system incorporating celecoxib as BCS class-II model drug. Attempts were made to prepare cost effective O/W microemulsion using Tween 80, Glycerol, sunflower oil and water. The existence of microemulsion zone was investigated using phase diagrams. The systems were characterized by polarized light microscopy, viscosity, refractive index, droplet size of dispersed phase by dynamic light scattering technique, thermal and centrifugal stability and the drug release profile. The obtained microemulsion was found optically isotropic with non-Newtonian behavior. The average droplet size was 100-300 nm. Microemulsions showed reversibility of transparency at ambient temperature after storage at 5 °C. The solubility enhancement of formulated products was apparent from higher release rate from microemulsion as compared to commercial product. The drug release profile was demonstrated to be promising for oral delivery of celecoxib. Galhardo Demarchi, Izabel Mioranza, Sônia de Lucena Teixeira, Jorge Juarez Vieira Takizawa, Maria G. Melito, Melina O. Mallmann, Larissa http://sedici.unlp.edu.ar:80/handle/10915/7812 2012-05-03T01:50:47Z 2009-01-01T00:00:00Z

Comunicacion Pharmacotherapeutic follow-up and adverse effects frequency at use of antiparasitics in health primary attention, Southwest of Paraná, Brazil Latin American Journal of Pharmacy; vol. 28, no. 4 O estudo teve como objetivo identificar a ocorrência de efeitos adversos durante o acompanhamento da terapia antiparasitária em indivíduos de um bairro de Cascavel – Paraná, Brasil, no período de março a junho de 2005. A Amostra foi constituída de 63 pacientes parasitados submetidos a quimioterapia. Dentre os 40 pacientes tratados, 27 apresentaram efeitos adversos, onde o Albendazol respondeu por 77,78% e o Metronidazol 22,22% dos casos. Todos os pacientes com diagnóstico positivo aderiram ao tratamento, sugerindo que a orientação farmacêutica continuada, enquanto processo educativo pode ter contribuído para a adesão a terapêutica prescrita.; The aim of this study was to identify the occurrence of adverse effects during the accompaniment of antiparasitics therapy in individuals of a neighbor at Cascavel – Parana, during from March to June of 2005. The samples was constituted of 63 individuals with parasitiases submitted to chemotherapy. Of the 40 patients treated, 27 showed side effects, with the Albendazole responsible for 77.78% and the Metronidazole 22.22% of the cases. All patients with positive diagnostic adhered to treatment, suggesting that the continued pharmaceutical orientation, while educative process, can have contributed with the adhesion to prescribed therapy.

2009-01-01T00:00:00Z O estudo teve como objetivo identificar a ocorrência de efeitos adversos durante o acompanhamento da terapia antiparasitária em indivíduos de um bairro de Cascavel – Paraná, Brasil, no período de março a junho de 2005. A Amostra foi constituída de 63 pacientes parasitados submetidos a quimioterapia. Dentre os 40 pacientes tratados, 27 apresentaram efeitos adversos, onde o Albendazol respondeu por 77,78% e o Metronidazol 22,22% dos casos. Todos os pacientes com diagnóstico positivo aderiram ao tratamento, sugerindo que a orientação farmacêutica continuada, enquanto processo educativo pode ter contribuído para a adesão a terapêutica prescrita. The aim of this study was to identify the occurrence of adverse effects during the accompaniment of antiparasitics therapy in individuals of a neighbor at Cascavel – Parana, during from March to June of 2005. The samples was constituted of 63 individuals with parasitiases submitted to chemotherapy. Of the 40 patients treated, 27 showed side effects, with the Albendazole responsible for 77.78% and the Metronidazole 22.22% of the cases. All patients with positive diagnostic adhered to treatment, suggesting that the continued pharmaceutical orientation, while educative process, can have contributed with the adhesion to prescribed therapy. Victório, Cristiane P. Kuster, Ricardo M. Lage, Celso Luiz S. http://sedici.unlp.edu.ar:80/handle/10915/7811 2012-05-03T01:50:47Z 2009-01-01T00:00:00Z

Comunicacion Latin American Journal of Pharmacy; vol. 28, no. 4 The accumulation of flavonoids was evaluated in organogenic cultures of Alpinia purpurata through HPLC analysis. Cultures were maintained in liquid MS medium in the following treatments: MS0 (control), TDZ, BAP and IAA + TDZ. Kaempferol-3-O-glucuronide content was higher than rutin for all in vitro treatments, except by control medium in which plants did not produce kaempferol-3-O-glucuronide. A remarkable increase in kaempferol-3-O-glucuronide content was verified using BAP 2 mg.l^-1 (0.027 mg/100 mg dried extract) and IAA 2 mg.l^-1 + TDZ 2 mg.l^-1 (0.030 mg/100 mg dried extract) treatments. With the addition of 2 mg.l^-1 BAP, rutin concentration also increased in the proportion 1:4 compared to control.

2009-01-01T00:00:00Z The accumulation of flavonoids was evaluated in organogenic cultures of Alpinia purpurata through HPLC analysis. Cultures were maintained in liquid MS medium in the following treatments: MS0 (control), TDZ, BAP and IAA + TDZ. Kaempferol-3-O-glucuronide content was higher than rutin for all in vitro treatments, except by control medium in which plants did not produce kaempferol-3-O-glucuronide. A remarkable increase in kaempferol-3-O-glucuronide content was verified using BAP 2 mg.l^-1 (0.027 mg/100 mg dried extract) and IAA 2 mg.l^-1 + TDZ 2 mg.l^-1 (0.030 mg/100 mg dried extract) treatments. With the addition of 2 mg.l^-1 BAP, rutin concentration also increased in the proportion 1:4 compared to control. Souza, Vagner H. Barbosa, Ana P.O. Cardoso, Geovanna C. Marreto, Ricardo N. Barreto Filho, José Augusto S. Antoniolli, Angelo R. Santos, Márcio R.V. http://sedici.unlp.edu.ar:80/handle/10915/7810 2012-05-03T01:50:47Z 2009-01-01T00:00:00Z

Comunicacion Evaluation of the antidiabetic potential of five medicinal plants in rats Latin American Journal of Pharmacy; vol. 28, no. 4 O presente estudo investigou o potencial antidiabético de extratos aquosos de cinco plantas medicinais indicadas popularmente para o tratamento do diabetes. Foram avaliados os efeitos anti-hiperglicemiantes agudos da metformina (500 mg/kg) e dos extratos da entrecasca de Bowdichia virgilioides Kunth (EABV), das folhas de Costus spicatus Swartz (EACS), das folhas de Chrysobalanus icaco L. (EACI), da entrecasca de Bumelia sartorum Mart (EABS) e da casca de Caesalpinia ferrea Mart (EACF) administrados em doses únicas de 200 mg/kg no teste oral de tolerância à glicose em ratos normoglicêmicos. Neste teste, somente o EABV, EACF e a metformina foram capazes de promover uma inibição da hiperglicemia induzida por sobrecarga de glicose em até 46,5, 51,7 e 48,3%, respectivamente. Estes resultados demonstram que das cinco espécies estudadas, duas apresentaram efeitos anti-hiperglicemiantes significativos que pode indicar uma possível ação antidiabética, o que reforça o indicativo popular.; Potential antidiabetic activity of five medicinal plants indicated to diabetes was evaluated in normoglicemic rats. Metformin (500 mg/kg) and aqueous extract of Bowdichia virgilioides Kunth (EABV), Costus spicatus Swartz (EACS), Chrysobalanus icaco L. (EACI), Bumelia sartorum Mart (EABS) and Caesalpinia ferrea Mart (EACF), in a single dose of 200 mg/kg, were tested on the curve of glucose tolerance oral test. In this test, only metformina, EABV and EACF were able to inhibit the glucose-induced hyperglycemia up to 46.5, 51.7 e 48.3%, respectively. In conclusion, of the five plants tested, two plants showed anti-hyperglycemic effect that can indicate possible antidiabetic action, in according with popular use.

2009-01-01T00:00:00Z O presente estudo investigou o potencial antidiabético de extratos aquosos de cinco plantas medicinais indicadas popularmente para o tratamento do diabetes. Foram avaliados os efeitos anti-hiperglicemiantes agudos da metformina (500 mg/kg) e dos extratos da entrecasca de Bowdichia virgilioides Kunth (EABV), das folhas de Costus spicatus Swartz (EACS), das folhas de Chrysobalanus icaco L. (EACI), da entrecasca de Bumelia sartorum Mart (EABS) e da casca de Caesalpinia ferrea Mart (EACF) administrados em doses únicas de 200 mg/kg no teste oral de tolerância à glicose em ratos normoglicêmicos. Neste teste, somente o EABV, EACF e a metformina foram capazes de promover uma inibição da hiperglicemia induzida por sobrecarga de glicose em até 46,5, 51,7 e 48,3%, respectivamente. Estes resultados demonstram que das cinco espécies estudadas, duas apresentaram efeitos anti-hiperglicemiantes significativos que pode indicar uma possível ação antidiabética, o que reforça o indicativo popular. Potential antidiabetic activity of five medicinal plants indicated to diabetes was evaluated in normoglicemic rats. Metformin (500 mg/kg) and aqueous extract of Bowdichia virgilioides Kunth (EABV), Costus spicatus Swartz (EACS), Chrysobalanus icaco L. (EACI), Bumelia sartorum Mart (EABS) and Caesalpinia ferrea Mart (EACF), in a single dose of 200 mg/kg, were tested on the curve of glucose tolerance oral test. In this test, only metformina, EABV and EACF were able to inhibit the glucose-induced hyperglycemia up to 46.5, 51.7 e 48.3%, respectively. In conclusion, of the five plants tested, two plants showed anti-hyperglycemic effect that can indicate possible antidiabetic action, in according with popular use. Silva, Fabiana E. B. da Ziech, Cristiane Pavoni, Gabriela D. Pasquali, Rosecler http://sedici.unlp.edu.ar:80/handle/10915/7809 2012-05-03T01:50:47Z 2009-01-01T00:00:00Z

Comunicacion Development of fluconazole tablets by direct compression Latin American Journal of Pharmacy; vol. 28, no. 4 O fluconazol é um membro da classe de agentes antifúngicos triazólicos amplamente utilizado na terapêutica para o tratamento de diferentes patologias causadas por fungos. A produção em grande escala de formas sólidas de uso oral está voltada para o desenvolvimento de comprimidos, devido ao menor custo da produção e grande aceitação desta forma farmacêutica por parte do consumidor. A escolha do método de produção é um fator importante em estudos de pré-formulação, sendo que o método da compressão direta oferece algumas vantagens como: rapidez na etapa de produção, custo reduzido quando comparado com métodos envolvendo granulação e eliminação de etapas, simplificando o processo como um todo. Entretanto, algumas características no desenvolvimento de formulações obtidas por compressão direta devem ser observadas, como características físicas dos comprimidos e perfil de dissolução, entre outros. O presente estudo tem como objetivo o desenvolvimento e avaliação de comprimidos de fluconazol obtidos por compressão direta.; Fluconazole is a member of an antifungal class agents widely used in therapy for treatment of diseases caused by fungi. The large scale production of oral solid forms is focused on the tablets development, due to the lower cost of production and wide acceptance of this form by the consumer. Direct compression method offers some advantages such as speed in the production stage, low cost when compared with methods involving granulation and production stage simplified. However, some features in formulations development obtained by direct compression must be observed as powders flow and dissolution profile, among others. The objective of this study is the development and evaluation of fluconazole tablets obtained by direct compression

2009-01-01T00:00:00Z O fluconazol é um membro da classe de agentes antifúngicos triazólicos amplamente utilizado na terapêutica para o tratamento de diferentes patologias causadas por fungos. A produção em grande escala de formas sólidas de uso oral está voltada para o desenvolvimento de comprimidos, devido ao menor custo da produção e grande aceitação desta forma farmacêutica por parte do consumidor. A escolha do método de produção é um fator importante em estudos de pré-formulação, sendo que o método da compressão direta oferece algumas vantagens como: rapidez na etapa de produção, custo reduzido quando comparado com métodos envolvendo granulação e eliminação de etapas, simplificando o processo como um todo. Entretanto, algumas características no desenvolvimento de formulações obtidas por compressão direta devem ser observadas, como características físicas dos comprimidos e perfil de dissolução, entre outros. O presente estudo tem como objetivo o desenvolvimento e avaliação de comprimidos de fluconazol obtidos por compressão direta. Fluconazole is a member of an antifungal class agents widely used in therapy for treatment of diseases caused by fungi. The large scale production of oral solid forms is focused on the tablets development, due to the lower cost of production and wide acceptance of this form by the consumer. Direct compression method offers some advantages such as speed in the production stage, low cost when compared with methods involving granulation and production stage simplified. However, some features in formulations development obtained by direct compression must be observed as powders flow and dissolution profile, among others. The objective of this study is the development and evaluation of fluconazole tablets obtained by direct compression Bacchi, Elfriede Marianne Okamoto, Marise K.H. Kato, Edna T. M. http://sedici.unlp.edu.ar:80/handle/10915/7808 2012-05-03T01:50:48Z 2009-01-01T00:00:00Z

Comunicacion Morphoanatomic aspects of Psidium guajava L. (Myrtaceae) leaves Latin American Journal of Pharmacy; vol. 28, no. 4 Psidium guajava L. é um arbusto perene, nativo da América tropical, cujas folhas são usadas na medicina tradicional latino-americana como antimicrobiano, antidiarreico, e auxiliar no tratamento de diabetes. As caracterizações morfoanatômica e histoquímica das folhas, realizadas neste trabalho, contribuem no controle de qualidade da matéria-prima vegetal desidratada, inteira ou em pó, empregada em formulações farmacêuticas. Os principais caracteres diagnósticos utilizados para a sua identificação neste trabalho foram: venação, tipo de tricomas, número de camadas de células epidérmicas, tipo de mesofilo e localização/tipo de cristais de oxalato de cálcio. A análise morfoanatômica mostra-se um método rápido para o controle de qualidade de drogas vegetais usadas na terapêutica.; Psidium guajava L. is a perennial shrub, native of tropical America used in Latin American traditional medicine as antimicrobial, antidiarrheic, and in the treatment of diabetes. The morphoanatomical characteristics and histochemistry of the crude drug, whole or powdered, used in pharmaceutical formulations were presented. The main diagnostic characters for the identification of its leaves in the present paper were: venation, trichomes type, number of layers of epidermal cells, mesophyll type and location/type of crystals of calcium oxalate. The morphoanatomical analysis has been proven to be a rapid method for quality control of the crude drug used for medicinal purposes.

2009-01-01T00:00:00Z Psidium guajava L. é um arbusto perene, nativo da América tropical, cujas folhas são usadas na medicina tradicional latino-americana como antimicrobiano, antidiarreico, e auxiliar no tratamento de diabetes. As caracterizações morfoanatômica e histoquímica das folhas, realizadas neste trabalho, contribuem no controle de qualidade da matéria-prima vegetal desidratada, inteira ou em pó, empregada em formulações farmacêuticas. Os principais caracteres diagnósticos utilizados para a sua identificação neste trabalho foram: venação, tipo de tricomas, número de camadas de células epidérmicas, tipo de mesofilo e localização/tipo de cristais de oxalato de cálcio. A análise morfoanatômica mostra-se um método rápido para o controle de qualidade de drogas vegetais usadas na terapêutica. Psidium guajava L. is a perennial shrub, native of tropical America used in Latin American traditional medicine as antimicrobial, antidiarrheic, and in the treatment of diabetes. The morphoanatomical characteristics and histochemistry of the crude drug, whole or powdered, used in pharmaceutical formulations were presented. The main diagnostic characters for the identification of its leaves in the present paper were: venation, trichomes type, number of layers of epidermal cells, mesophyll type and location/type of crystals of calcium oxalate. The morphoanatomical analysis has been proven to be a rapid method for quality control of the crude drug used for medicinal purposes. Carli, Roseni B.G. de Siqueira, Paula R.A. Kaiser, Marina L. Freitas, Rilton A. Souza, Márcia María de Cechinel Filho, Valdir Lucinda da Silva, Ruth Meri http://sedici.unlp.edu.ar:80/handle/10915/7807 2012-05-03T01:50:48Z 2009-01-01T00:00:00Z

Comunicacion Latin American Journal of Pharmacy; vol. 28, no. 4 The aim of this work is to evaluate in vivo the anti-inflammatory effect of creams containing kaurenoic acid (KA), isolated from the acetonic extract of the W. paludosa (stems and roots). The herbal drug was incorporated into anionic cream (Lanette®) at 100 μg/g. Creams containing different permeation enhancers (urea, alpha bisabolol, isodecyl oleate, isopropyl myristate, soy lecithin) were prepared, and the in vivo topical anti-inflammatory effect was evaluated by the croton oil-induced ear edema method in mice using dexamethasone cream (5 mg/g) and Acheflan® (essential oil of Cordia verbenacea 5 mg/g) as positive control. The potential cutaneous irritation was evaluated by the agarose overlay assay. KA cream, KA and isopropyl myristate and soy lecithin cream and dexamethasone cream presented inhibition of ear edema of 61.73 ± 23.23%, 71.71 ± 15.77% and 64.45 ± 13.41%, respectively. These results suggest that KA incorporated in the cream showed a greater anti-inflammatory effect than positive control, while KA cream containing a concentration lower than dexamethasone cream presents a statistically similar ear edema reduction compared with the control, with no potential cutaneous irritation being observed.

2009-01-01T00:00:00Z The aim of this work is to evaluate in vivo the anti-inflammatory effect of creams containing kaurenoic acid (KA), isolated from the acetonic extract of the W. paludosa (stems and roots). The herbal drug was incorporated into anionic cream (Lanette®) at 100 μg/g. Creams containing different permeation enhancers (urea, alpha bisabolol, isodecyl oleate, isopropyl myristate, soy lecithin) were prepared, and the in vivo topical anti-inflammatory effect was evaluated by the croton oil-induced ear edema method in mice using dexamethasone cream (5 mg/g) and Acheflan® (essential oil of Cordia verbenacea 5 mg/g) as positive control. The potential cutaneous irritation was evaluated by the agarose overlay assay. KA cream, KA and isopropyl myristate and soy lecithin cream and dexamethasone cream presented inhibition of ear edema of 61.73 ± 23.23%, 71.71 ± 15.77% and 64.45 ± 13.41%, respectively. These results suggest that KA incorporated in the cream showed a greater anti-inflammatory effect than positive control, while KA cream containing a concentration lower than dexamethasone cream presents a statistically similar ear edema reduction compared with the control, with no potential cutaneous irritation being observed. Barbosa, Vanessa de F. Isaac, Vera L.B. Oliveira, Olga M.M. de F. Cefali, Letícia C. Rinaldo, Daniel Salgado, Hérida R.N. Vilegas, Wagner http://sedici.unlp.edu.ar:80/handle/10915/7806 2012-05-03T01:50:48Z 2009-01-01T00:00:00Z

Comunicacion Tomato salad: an alternative as antioxidant source for topic use Latin American Journal of Pharmacy; vol. 28, no. 4 O tomate é a principal fonte de licopeno que é um carotenóide com alta atividade antioxidante, podendo assim ser utilizado topicamente na forma de um fitocosmético no combate ao envelhecimento cutâneo. O objetivo deste estudo foi obter um extrato rico em licopeno através da polpa do tomate tipo salada. O extrato foi analisado utilizando os métodos de espectroscopia no ultravioleta/visível, cromatografia de camada delgada e cromatografia líquida de alta eficiência. A atividade antioxidante do extrato foi avaliada utilizando o método do radical livre DPPH. Foi identificada a presença de licopeno na polpa do tomate salada e o extrato apresentou uma fração apolar rica em carotenóides referente a 96,70% de licopeno. Na avaliação da atividade antioxidante usando o radical DPPH, o extrato apresentou atividade (IC₅₀ de 0,311 mg/mL). Nós concluímos que o tomate salada é uma fonte rica em licopeno e pode ser utilizado futuramente como antioxidante para uso tópico; Tomato is the principal source of lycopene, a carotenoid with high antioxidant activity which can be used topically as a phytocosmetic to combat skin aging. The aim of this study was to get a lycopene-rich extract from salad tomato pulp. The extract was analyzed using UV/vis spectroscopy, thin layer chromatography and high performance liquid chromatography. Oxidant activity was analyzed using the free radical DPPH method. The presence of lycopene in the salad tomato was confirmed. It was present in the apolar fraction rich in carotenoids, which was 96.70% lycopene. In the evaluation of the antioxidant activity, using the radical DPPH, the extract showed activity (IC₅₀ = 0,311 mg/mL). We conclude that the salad tomato can be used as a source of antioxidants for topic use.

2009-01-01T00:00:00Z O tomate é a principal fonte de licopeno que é um carotenóide com alta atividade antioxidante, podendo assim ser utilizado topicamente na forma de um fitocosmético no combate ao envelhecimento cutâneo. O objetivo deste estudo foi obter um extrato rico em licopeno através da polpa do tomate tipo salada. O extrato foi analisado utilizando os métodos de espectroscopia no ultravioleta/visível, cromatografia de camada delgada e cromatografia líquida de alta eficiência. A atividade antioxidante do extrato foi avaliada utilizando o método do radical livre DPPH. Foi identificada a presença de licopeno na polpa do tomate salada e o extrato apresentou uma fração apolar rica em carotenóides referente a 96,70% de licopeno. Na avaliação da atividade antioxidante usando o radical DPPH, o extrato apresentou atividade (IC₅₀ de 0,311 mg/mL). Nós concluímos que o tomate salada é uma fonte rica em licopeno e pode ser utilizado futuramente como antioxidante para uso tópico Tomato is the principal source of lycopene, a carotenoid with high antioxidant activity which can be used topically as a phytocosmetic to combat skin aging. The aim of this study was to get a lycopene-rich extract from salad tomato pulp. The extract was analyzed using UV/vis spectroscopy, thin layer chromatography and high performance liquid chromatography. Oxidant activity was analyzed using the free radical DPPH method. The presence of lycopene in the salad tomato was confirmed. It was present in the apolar fraction rich in carotenoids, which was 96.70% lycopene. In the evaluation of the antioxidant activity, using the radical DPPH, the extract showed activity (IC₅₀ = 0,311 mg/mL). We conclude that the salad tomato can be used as a source of antioxidants for topic use. García Peña, Caridad M. León, Rafael Baró Román, Gerardo Martínez Espinosa, Vivian http://sedici.unlp.edu.ar:80/handle/10915/7805 2012-05-03T01:50:48Z 2009-01-01T00:00:00Z

Comunicacion Comparative evaluation of the In vitro release of a Metformin 500 mg preparation made in Cuba against Glucophage® Latin American Journal of Pharmacy; vol. 28, no. 4 Se compararon los perfiles de disolución de las tabletas de Metformina 500 mg de producción cubana y de Glucophage® (Laboratorios Bristol-Myers Squibb Co.), para demostrar su similitud. También se realizó la comparación en varios medios de disolución a diferentes pH para evaluar una posible bioexoneración. Para la cuantificación del principio activo se utilizó un método por CLAR previamente validado. La comparación se realizó en base a los factores de diferenciación y similitud, los resultados mostraron que no existen diferencias en los perfiles de liberación para los ensayos de producción nacional y del producto innovador, así como, para los diferentes medios de disolución a los pH utilizados.; Dissolution's profiles of Metformina 500 mg tablets (CIDEM, Cuba) and Glucophage® (Bristol-Myers Squibb Co. Laboratories) were compared to demonstrate their interchangeability. A comparison was also developed using several dissolution mediums, with different pH values, in order to evaluate a possible bioexoneration. A previously validated HPLC method was used to quantify the active principle and the comparison was carried out on the basis of differentiation and similarity factors. Results showed no difference between the release profiles of both formulations, for the different dissolution mediums assayed.

2009-01-01T00:00:00Z Se compararon los perfiles de disolución de las tabletas de Metformina 500 mg de producción cubana y de Glucophage® (Laboratorios Bristol-Myers Squibb Co.), para demostrar su similitud. También se realizó la comparación en varios medios de disolución a diferentes pH para evaluar una posible bioexoneración. Para la cuantificación del principio activo se utilizó un método por CLAR previamente validado. La comparación se realizó en base a los factores de diferenciación y similitud, los resultados mostraron que no existen diferencias en los perfiles de liberación para los ensayos de producción nacional y del producto innovador, así como, para los diferentes medios de disolución a los pH utilizados. Dissolution's profiles of Metformina 500 mg tablets (CIDEM, Cuba) and Glucophage® (Bristol-Myers Squibb Co. Laboratories) were compared to demonstrate their interchangeability. A comparison was also developed using several dissolution mediums, with different pH values, in order to evaluate a possible bioexoneration. A previously validated HPLC method was used to quantify the active principle and the comparison was carried out on the basis of differentiation and similarity factors. Results showed no difference between the release profiles of both formulations, for the different dissolution mediums assayed. Bomfim, Rangel R. Araújo, Adriano A. S. Cuadros-Orellana, Sara Melo, Marcélia G.D. Quintans Júnior, Lucindo José Cavalcanti, Sócrates C.H. http://sedici.unlp.edu.ar:80/handle/10915/7804 2012-05-03T01:50:48Z 2009-01-01T00:00:00Z

Comunicacion Latin American Journal of Pharmacy; vol. 28, no. 4 The chloroform extract of Cladonia substellata Vainio was assayed against larvae of Aedes aegypti, the mosquito vector of Dengue fever and Artemia salina. The extract was tested at concentrations ranging from 1 to 15 ppm in an aqueous medium for 24 h. LC₅₀ and LC₉₀ were evaluated. Since the chloroform extract proved to be lethal for third to fourth instar larvae, downstream processing was undertaken to purify the active agents in the extract. The major compound in the chloroform extract was purified by crystallization followed by column chromatography to yield yellow crystals. Furthermore, usnic acid (UA) was evaluated for its larvicidal potential. The major compound in the chloroform extract, UA, exhibited LC₅₀ of 6.6 ppm (6.1 to 7.0 ppm). Therefore, UA is most likely the active principle in C. substellata. UA showed to be toxic to A. salina, a reference organism in assays to evaluate the potential toxicity hazard to invertebrates in ecosystems.

2009-01-01T00:00:00Z The chloroform extract of Cladonia substellata Vainio was assayed against larvae of Aedes aegypti, the mosquito vector of Dengue fever and Artemia salina. The extract was tested at concentrations ranging from 1 to 15 ppm in an aqueous medium for 24 h. LC₅₀ and LC₉₀ were evaluated. Since the chloroform extract proved to be lethal for third to fourth instar larvae, downstream processing was undertaken to purify the active agents in the extract. The major compound in the chloroform extract was purified by crystallization followed by column chromatography to yield yellow crystals. Furthermore, usnic acid (UA) was evaluated for its larvicidal potential. The major compound in the chloroform extract, UA, exhibited LC₅₀ of 6.6 ppm (6.1 to 7.0 ppm). Therefore, UA is most likely the active principle in C. substellata. UA showed to be toxic to A. salina, a reference organism in assays to evaluate the potential toxicity hazard to invertebrates in ecosystems. Queiroz, Maria B. R. Marcelino, Natália B. Ribeiro, Marcos V. Espindola, Laila S. Cunha, Francisco R. Silva, Mônica V. da http://sedici.unlp.edu.ar:80/handle/10915/7803 2012-05-03T01:50:48Z 2009-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 28, no. 4 Matricaria recutita L. (Asteraceae), better known as chamomile, has been used due to its pharmacological properties. Laboratory-manufactured gels with chamomile extract were developed with the evaluation of the physical-chemical stability, as well as the study of its toxicity. The extractive solution was prepared by maceration with ethyl alcohol 95%. Part of the chamomile extractive ethanolic solution (CEES) was concentrated in rotoevaporator, obtaining a raw chamomile extract (RCE). For the preparation of gels, carbopol 940P, hydroxyethyl cellulose and hydroxypropyl methylcellulose were used with the addition of 3% and 5% chamomile extracts. The stability tests applied to the gels were as such: thermal stress, pH evaluation, viscosity and storage at different temperatures. In the end of the tests it was observed that the carbopol gel was the most stable. The Draize Test was employed as the toxicity test, with no irritation observed; however, on skin that underwent abrasion, some gels caused a little irritation.

2009-01-01T00:00:00Z Matricaria recutita L. (Asteraceae), better known as chamomile, has been used due to its pharmacological properties. Laboratory-manufactured gels with chamomile extract were developed with the evaluation of the physical-chemical stability, as well as the study of its toxicity. The extractive solution was prepared by maceration with ethyl alcohol 95%. Part of the chamomile extractive ethanolic solution (CEES) was concentrated in rotoevaporator, obtaining a raw chamomile extract (RCE). For the preparation of gels, carbopol 940P, hydroxyethyl cellulose and hydroxypropyl methylcellulose were used with the addition of 3% and 5% chamomile extracts. The stability tests applied to the gels were as such: thermal stress, pH evaluation, viscosity and storage at different temperatures. In the end of the tests it was observed that the carbopol gel was the most stable. The Draize Test was employed as the toxicity test, with no irritation observed; however, on skin that underwent abrasion, some gels caused a little irritation. Lu, Wenzong Yang, Yali Li, Qingwang Liu, Fuzhu http://sedici.unlp.edu.ar:80/handle/10915/7802 2012-05-03T01:50:48Z 2009-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 28, no. 4 The aim of this study was to investigate the anticancer properties of crude flavonoids from Carya cathayensis Sargent bark (CCS-F), including the inhibitory effect of CCS-F on HeLa cells and the apoptosis-inducing capacity in vitro. In our results, treatment of HeLa cells with CCS-F resulted in the growth inhibition effect, and the IC50 was 95 μg/ml. Detection of apoptosis was performed by acridine orange / ethidium bromide (AO/EB) and Tdt-mediated dUTP nick end labeling (TUNEL) staining assays, which showed more apoptosis cells in CCS-F treatment group than the control group. Furthermore, CCSF (100 μg/ml) could arrest the cells in G0/G1 phase. Meanwhile, the expression of Bax was increased in the cells treated with CCS-F (100 μg/ml), with an increase in the activity of caspase-3, while Bcl-2 expression was decreased compared to the control cells. It demonstrated that CCS-F had antiproliferative activity in HeLa cells and might be a potential anticancer drug.

2009-01-01T00:00:00Z The aim of this study was to investigate the anticancer properties of crude flavonoids from Carya cathayensis Sargent bark (CCS-F), including the inhibitory effect of CCS-F on HeLa cells and the apoptosis-inducing capacity in vitro. In our results, treatment of HeLa cells with CCS-F resulted in the growth inhibition effect, and the IC50 was 95 μg/ml. Detection of apoptosis was performed by acridine orange / ethidium bromide (AO/EB) and Tdt-mediated dUTP nick end labeling (TUNEL) staining assays, which showed more apoptosis cells in CCS-F treatment group than the control group. Furthermore, CCSF (100 μg/ml) could arrest the cells in G0/G1 phase. Meanwhile, the expression of Bax was increased in the cells treated with CCS-F (100 μg/ml), with an increase in the activity of caspase-3, while Bcl-2 expression was decreased compared to the control cells. It demonstrated that CCS-F had antiproliferative activity in HeLa cells and might be a potential anticancer drug. Alza, Natalia P. Cambi, Viviana N. http://sedici.unlp.edu.ar:80/handle/10915/7801 2012-05-03T01:50:48Z 2009-01-01T00:00:00Z

Articulo Quality control of plant mixtures commercialized as sedatives in Bahia Blanca, Argentina Latin American Journal of Pharmacy; vol. 28, no. 4 El consumo de plantas con fines medicinales, práctica tradicional desde tiempos inmemorables, se ha incrementado en los últimos años con respecto a anteriores. Esta práctica no parece acompañarse de un incremento en el control estatal de calidad de productos que incluyen plantas medicinales. Por esta razón se realizó un análisis de rótulos e identificación de los componentes vegetales de las mezclas sedantes comercializadas en Bahía Blanca, por ser las mismas de uso ampliamente difundido. Se relevaron todas las farmacias y principales herboristerías de la ciudad, hallándose cinco marcas comerciales. Las irregularidades observadas en los rótulos son variadas. A partir del análisis macroscópico y micrográfico, se hallaron adulteraciones de algún tipo en la totalidad de las mezclas.; The consumption of plants with medicinal purposes, traditional practice from immemorial times, has increased in the last years with regard to previous. This practice does not seem to be accompanied of an increase in the state quality control of products that include medicinal plants. For this reason we propose to realize an analysis of labels and identification of the vegetable components of the sedative mixtures that are commercialized in Bahía Blanca for being one of those of more spread use. There were relieved all the drugstores and principal herbalists of the city, being recognized five commercial marks. The irregularities observed in the labels are varied. From macroscopic and micrographycal analysis, adulterations of some type were found in all mixtures.

2009-01-01T00:00:00Z El consumo de plantas con fines medicinales, práctica tradicional desde tiempos inmemorables, se ha incrementado en los últimos años con respecto a anteriores. Esta práctica no parece acompañarse de un incremento en el control estatal de calidad de productos que incluyen plantas medicinales. Por esta razón se realizó un análisis de rótulos e identificación de los componentes vegetales de las mezclas sedantes comercializadas en Bahía Blanca, por ser las mismas de uso ampliamente difundido. Se relevaron todas las farmacias y principales herboristerías de la ciudad, hallándose cinco marcas comerciales. Las irregularidades observadas en los rótulos son variadas. A partir del análisis macroscópico y micrográfico, se hallaron adulteraciones de algún tipo en la totalidad de las mezclas. The consumption of plants with medicinal purposes, traditional practice from immemorial times, has increased in the last years with regard to previous. This practice does not seem to be accompanied of an increase in the state quality control of products that include medicinal plants. For this reason we propose to realize an analysis of labels and identification of the vegetable components of the sedative mixtures that are commercialized in Bahía Blanca for being one of those of more spread use. There were relieved all the drugstores and principal herbalists of the city, being recognized five commercial marks. The irregularities observed in the labels are varied. From macroscopic and micrographycal analysis, adulterations of some type were found in all mixtures. Del Vitto, Luis A. Petenatti, Elisa M. Petenatti, Marta E. Mazza, Silvia M. Marchevsky, Eduardo J. http://sedici.unlp.edu.ar:80/handle/10915/7800 2012-05-03T01:50:48Z 2009-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 28, no. 4 Multielement analysis of crude drug and infusions from Achyrocline satureioides and A. tomentosa (Asteraceae), "marcelas", were carried out by ICP-OES, to know both mineral composition and safety. These plants are used as herbal remedies and extracts for bitter beverages in southern South America. Twenty seven major and trace elements were determined. Crude drugs contained valuable amounts of K, Ca, Mg, and P and essential trace elements (Fe, Mn, Zn, and Cu). Variability in the passage of minerals towards infusions was observed, too, and concentrations of K, Ca, P, and Mg were higher than those of Na, Mn, Zn, Cu, Al, Fe, Ni, Li, Hg and Mo, while other minerals were not detected. The infusions would be safe for human consumption because remain within the limits of the recommended daily intake or tolerable upper intake, and contribute to the daily intake in the case of some essential minerals.

2009-01-01T00:00:00Z Multielement analysis of crude drug and infusions from Achyrocline satureioides and A. tomentosa (Asteraceae), "marcelas", were carried out by ICP-OES, to know both mineral composition and safety. These plants are used as herbal remedies and extracts for bitter beverages in southern South America. Twenty seven major and trace elements were determined. Crude drugs contained valuable amounts of K, Ca, Mg, and P and essential trace elements (Fe, Mn, Zn, and Cu). Variability in the passage of minerals towards infusions was observed, too, and concentrations of K, Ca, P, and Mg were higher than those of Na, Mn, Zn, Cu, Al, Fe, Ni, Li, Hg and Mo, while other minerals were not detected. The infusions would be safe for human consumption because remain within the limits of the recommended daily intake or tolerable upper intake, and contribute to the daily intake in the case of some essential minerals. Nounou, Bárbara Cattáneo, María E. Salmón, Romina Palasezze, Leonardo Boccaleri, Julieta Cestona, Eugenia Bedecarrás, Florencia Ranieri, Florencia Talevi, Alan Muñoz, Susana M. http://sedici.unlp.edu.ar:80/handle/10915/7799 2012-05-03T01:50:48Z 2009-01-01T00:00:00Z

Articulo A Study regarding consumption and self-medication with antibiotics in the city of La Plata (Buenos Aires, Argentina) Latin American Journal of Pharmacy; vol. 28, no. 4 La auto-medicación con antibióticos (ATBs) es un problema sanitario de importancia mayor a nivel mundial. La continua aparición de cepas bacterianas resistentes a los ATBs conocidos da cuenta de las consecuencias del uso injustificado de estos agentes terapéuticos, ya sea debido a una prescripción médica errónea, uso inadecuado por auto-medicación o falta de cumplimiento del tratamiento prescrito. A fin de estudiar las conductas de consumo y auto-medicación con ATBs en la Ciudad de La Plata se llevó a cabo una encuesta a 482 habitantes de dicha ciudad. Los resultados indican que aunque la auto-medicación con ATBs es percibida como una conducta riesgosa por la población encuestada, el 53 % de la misma consume, al menos ocasionalmente, ATBs sin prescripción médica, debido principalmente a falta de tiempo y a la percepción de que no siempre es necesaria una consulta al médico para diagnosticar la enfermedad y elegir el tratamiento adecuado. Se comparan los resultados con los de otros estudios anteriores llevados a cabo en Latinoamérica y en el resto del mundo.; Self-medication with antibiotics (ATBs) is a major, global sanitary problem. The continuous appearance of bacterial strains resistant to known ATBs denotes the consequences of the unjustified use of these therapeutic agents, whether the unjustified use is due to a wrong physician prescription, self-medication or lack of adherence to the prescript treatment. In order to study the conduits of consumption of and self medication with ATBs in the City of La Plata, we have carried out a survey to 482 inhabitants of this city. Results indicate that, although self-prescription with ATBs is perceived mostly as a risky conduit in the surveyed population, 53% of it consumes, at least occasionally, ATBs without medical prescription, mainly due to lack of time and the perception that a visit to the physician is not always necessary to diagnose the disease and choose the adequate treatment. Results are compared to those of previous studies performed in Latin America and the rest of the world

2009-01-01T00:00:00Z La auto-medicación con antibióticos (ATBs) es un problema sanitario de importancia mayor a nivel mundial. La continua aparición de cepas bacterianas resistentes a los ATBs conocidos da cuenta de las consecuencias del uso injustificado de estos agentes terapéuticos, ya sea debido a una prescripción médica errónea, uso inadecuado por auto-medicación o falta de cumplimiento del tratamiento prescrito. A fin de estudiar las conductas de consumo y auto-medicación con ATBs en la Ciudad de La Plata se llevó a cabo una encuesta a 482 habitantes de dicha ciudad. Los resultados indican que aunque la auto-medicación con ATBs es percibida como una conducta riesgosa por la población encuestada, el 53 % de la misma consume, al menos ocasionalmente, ATBs sin prescripción médica, debido principalmente a falta de tiempo y a la percepción de que no siempre es necesaria una consulta al médico para diagnosticar la enfermedad y elegir el tratamiento adecuado. Se comparan los resultados con los de otros estudios anteriores llevados a cabo en Latinoamérica y en el resto del mundo. Self-medication with antibiotics (ATBs) is a major, global sanitary problem. The continuous appearance of bacterial strains resistant to known ATBs denotes the consequences of the unjustified use of these therapeutic agents, whether the unjustified use is due to a wrong physician prescription, self-medication or lack of adherence to the prescript treatment. In order to study the conduits of consumption of and self medication with ATBs in the City of La Plata, we have carried out a survey to 482 inhabitants of this city. Results indicate that, although self-prescription with ATBs is perceived mostly as a risky conduit in the surveyed population, 53% of it consumes, at least occasionally, ATBs without medical prescription, mainly due to lack of time and the perception that a visit to the physician is not always necessary to diagnose the disease and choose the adequate treatment. Results are compared to those of previous studies performed in Latin America and the rest of the world Karmarkar, Amrit B. Gonjari, Indrajeet D. Dhabale, Pandurang N. Bhise, Satish B. http://sedici.unlp.edu.ar:80/handle/10915/7798 2012-05-03T01:50:48Z 2009-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 28, no. 4 The present work deals with the comparison of in vitro dissolution profiles of fenofibrate liquisolid tablet formulations with those of marketed fenofibrate tablets, and the application of statistical methods to evaluate each method for its usefulness. The methods used to study dissolution profile comparison include Model independent method (Similarity factor, f2); Model dependent methods (Zero order, First order, Hixson-Crowell, Matrix, Peppas, Higuchi models) and statistical methods based on ANOVA. Model independent method was found to be easier and simple to interpret. The f2 value relates closeness of dissolution profiles. Dissolution profile followed Peppas model as "best fit" model. The application and evaluation of model dependent methods are more complicated. These methods give acceptable model approach which is indication of true relationship between percent drug release and time variables, including statistical assumptions. Statistical approach is very simple and is more discriminative of dissolution profiles. The liquisolid formulation of fenofibrate serves to be an effective way to enhance dissolution rate of fenofibrate

2009-01-01T00:00:00Z The present work deals with the comparison of in vitro dissolution profiles of fenofibrate liquisolid tablet formulations with those of marketed fenofibrate tablets, and the application of statistical methods to evaluate each method for its usefulness. The methods used to study dissolution profile comparison include Model independent method (Similarity factor, f2); Model dependent methods (Zero order, First order, Hixson-Crowell, Matrix, Peppas, Higuchi models) and statistical methods based on ANOVA. Model independent method was found to be easier and simple to interpret. The f2 value relates closeness of dissolution profiles. Dissolution profile followed Peppas model as "best fit" model. The application and evaluation of model dependent methods are more complicated. These methods give acceptable model approach which is indication of true relationship between percent drug release and time variables, including statistical assumptions. Statistical approach is very simple and is more discriminative of dissolution profiles. The liquisolid formulation of fenofibrate serves to be an effective way to enhance dissolution rate of fenofibrate Barros, Gilvana C. Tresvenzol, Leonice M.F. Cunha, Luiz C. Ferri, Pedro H. Paula, José R. Bara, María Teresa. F. http://sedici.unlp.edu.ar:80/handle/10915/7797 2012-05-03T01:50:49Z 2009-01-01T00:00:00Z

Articulo Chemical composition, antibacterial activity and evaluation of acute toxicity of vetiveria zizanoides L. Nash (Poaceae) Latin American Journal of Pharmacy; vol. 28, no. 4 O vetiver (Vetiveria zizanioides L. Nash) é uma planta aromática representante da família Poaceae e utilizada na terapia Ayurvédica. Nesse trabalho objetivou-se investigar a composição química do óleo essencial da raiz do vetiver cultivado no Hospital de Medicina Alternativa em Goiás-Brasil, avaliar seu potencial antimicrobiano e realizar ensaio de toxicidade aguda. A análise química do óleo essencial foi realizada por cromatografia gasosa acoplada a espectrometria de massas (CG/EM), e o khusimol (12,86 %) e o palustrol (8,67 %) foram os componentes majoritários. Na avaliação do potencial antimicrobiano do óleo essencial de V. zizanioides foi observada potente atividade contra Micrococcus roseus, com concentração inibitória mínima (CIM) de 70 μg/mL e contra Staphylococcus aureus com CIM de 150 μg/mL. O extrato etanólico bruto apresentou atividade antimicrobiana superior à s frações, com CIM de 190 μg/mL para M. roseus e Bacillus cereus. Na avaliação da toxicidade aguda via oral verificou-se que o extrato etanólico bruto não apresentou toxicidade para ratos na dose de 2000 mg/kg; The vetiver (Vetiver zizanioides L. Nash) is a representative aromatic plant of the family Poaceae and used in Ayurvedic therapy. This work aimed to investigate the chemical composition of essential oil of the roots of vetiver grown in the Hospital of Alternative Medicine in Goiás, Brazil, to evaluate potential antimicrobial and perform acute toxicity test. In the analysis of the essential oil khusimol (12, 86%) and palustrol (8,67%) were the major components. In evaluating the antimicrobial potential of essential oil of V. zizanioides it was noted a potent activity against Micrococcus roseus, with minimum inhibitory concentration (MIC) of 70 μg/mL and against Staphylococcus aureus with MIC of 150 μg/mL. The crude ethanolic extract showed antimicrobial activity greater than the fractions, with MIC of 190 μg/mL for M. roseus and Bacillus cereus. In assessing the acute oral toxicity it was found that the crude ethanolic extract showed no toxicity to rats at a dose of 2000 mg/kg

2009-01-01T00:00:00Z O vetiver (Vetiveria zizanioides L. Nash) é uma planta aromática representante da família Poaceae e utilizada na terapia Ayurvédica. Nesse trabalho objetivou-se investigar a composição química do óleo essencial da raiz do vetiver cultivado no Hospital de Medicina Alternativa em Goiás-Brasil, avaliar seu potencial antimicrobiano e realizar ensaio de toxicidade aguda. A análise química do óleo essencial foi realizada por cromatografia gasosa acoplada a espectrometria de massas (CG/EM), e o khusimol (12,86 %) e o palustrol (8,67 %) foram os componentes majoritários. Na avaliação do potencial antimicrobiano do óleo essencial de V. zizanioides foi observada potente atividade contra Micrococcus roseus, com concentração inibitória mínima (CIM) de 70 μg/mL e contra Staphylococcus aureus com CIM de 150 μg/mL. O extrato etanólico bruto apresentou atividade antimicrobiana superior à s frações, com CIM de 190 μg/mL para M. roseus e Bacillus cereus. Na avaliação da toxicidade aguda via oral verificou-se que o extrato etanólico bruto não apresentou toxicidade para ratos na dose de 2000 mg/kg The vetiver (Vetiver zizanioides L. Nash) is a representative aromatic plant of the family Poaceae and used in Ayurvedic therapy. This work aimed to investigate the chemical composition of essential oil of the roots of vetiver grown in the Hospital of Alternative Medicine in Goiás, Brazil, to evaluate potential antimicrobial and perform acute toxicity test. In the analysis of the essential oil khusimol (12, 86%) and palustrol (8,67%) were the major components. In evaluating the antimicrobial potential of essential oil of V. zizanioides it was noted a potent activity against Micrococcus roseus, with minimum inhibitory concentration (MIC) of 70 μg/mL and against Staphylococcus aureus with MIC of 150 μg/mL. The crude ethanolic extract showed antimicrobial activity greater than the fractions, with MIC of 190 μg/mL for M. roseus and Bacillus cereus. In assessing the acute oral toxicity it was found that the crude ethanolic extract showed no toxicity to rats at a dose of 2000 mg/kg Alcântara, Adélia M.P.P. Venuto, Liliane M.A. França, Ana L.F. Vieira, Elisabeth Martins, Isarita http://sedici.unlp.edu.ar:80/handle/10915/7796 2012-05-03T01:50:49Z 2009-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 28, no. 4 Therapeutic importance and benefices caused by antineoplastic drugs are unquestionable however unfortunately well-known are their side effects. So, the extensive use and the exposure to multiple agents may be at risk to health care workers involved in the preparation and administration of these drugs. It is therefore important to have accurate methods for simultaneous analysis for evaluation of the occupational exposure. In this study, we have developed a method for simultaneous determination of 5-fluorouracil (5-FU), methotrexate (MTX), doxorubicin (DOX), cyclophosphamide (CP) and ifosfamide (IF). The assay was performed by HPLC-UV, detection in 195 nm, with a C18 column (250 x 4 mm, 5 μm) with a similar guard- column. Mobile phase was constituted by water pH 4: acetonitrile: methanol (70:17:13, v/v/v) with a flow of 0.4 mL min–1 up to 13 min and after this, 1 mL min–1 For cleaning of surfaces, we . used a solution of acetonitrile: methanol (50:50, v/v). The method presented a linear calibration in a range from 0.25 to 20 μg mL–1 , for 5-FU and MTX and from 0.5 to 20 μg mL–1 for IF, DOX and CP, with correlation coefficients (r2 ) upper to 0.997. The repeatability, expressed in terms of percent relative standard deviation, was ≤ 10% and recovery was > 70%, in surfaces contaminated with the analytes. The results obtained suggest that the method developed can be applicable for simultaneous determination of the five drugs studied and can be considered useful in exposure assessment

2009-01-01T00:00:00Z Therapeutic importance and benefices caused by antineoplastic drugs are unquestionable however unfortunately well-known are their side effects. So, the extensive use and the exposure to multiple agents may be at risk to health care workers involved in the preparation and administration of these drugs. It is therefore important to have accurate methods for simultaneous analysis for evaluation of the occupational exposure. In this study, we have developed a method for simultaneous determination of 5-fluorouracil (5-FU), methotrexate (MTX), doxorubicin (DOX), cyclophosphamide (CP) and ifosfamide (IF). The assay was performed by HPLC-UV, detection in 195 nm, with a C18 column (250 x 4 mm, 5 μm) with a similar guard- column. Mobile phase was constituted by water pH 4: acetonitrile: methanol (70:17:13, v/v/v) with a flow of 0.4 mL min–1 up to 13 min and after this, 1 mL min–1 For cleaning of surfaces, we . used a solution of acetonitrile: methanol (50:50, v/v). The method presented a linear calibration in a range from 0.25 to 20 μg mL–1 , for 5-FU and MTX and from 0.5 to 20 μg mL–1 for IF, DOX and CP, with correlation coefficients (r2 ) upper to 0.997. The repeatability, expressed in terms of percent relative standard deviation, was ≤ 10% and recovery was > 70%, in surfaces contaminated with the analytes. The results obtained suggest that the method developed can be applicable for simultaneous determination of the five drugs studied and can be considered useful in exposure assessment