vol. 29, no. 01http://sedici.unlp.edu.ar:80/handle/10915/2262024-03-19T13:05:51Z2024-03-19T13:05:51ZAvaliação da linearidade de métodos analíticos para o radiofármaco fluorodesoxiglicose-18Santos Oliveira, RalphFernandes, Ricardo R. A.http://sedici.unlp.edu.ar:80/handle/10915/78882019-06-28T04:02:38Z2010-01-01T00:00:00ZComunicacion
Linearity evaluation of analytical methods of radiopharmaceutical fluorodeoxyglucose-18
Latin American Journal of Pharmacy; vol. 29, no. 1
Radiofármacos são medicamentos utilizados na Medicina Nuclear para avaliar funções biológicas
e patofisiológicas, realizar estudos do funcionamento do cérebro e diversas outras enfermidades, assim
comodesenvolver uma terapia eficaz. Tomografia por emissão de pósitron e tomografia computadorizada
por emissão de fóton único são alguns dos métodos que utilizam radiofármacos. Desenvolvimento de novos
radiofármacos é a solução para a análise in vivo de imagens endógenas. Contudo não só o desenvolvimento
é importante. A qualidade e o comportamento do radiofármaco desenvolvido devem ser comprovadas ,
diariamente. Nesse trabalho os autores avaliam a metodologia de determinação de etanol e do teor de F-18
e FDG-18 como forma de avaliação da qualidade do produto final, e dos método utilizados, assim como
dos equipamentos. Os resultados revelam que a metodologia de determinação de etanol em solução de
FDG-18 deve ser revista. Contudo a análise de teor de F-18 e FDG-18, mostrou-se bastante eficaz.; Radiopharmaceuticals
are drugs used in Nuclear Medicine to measure physiological and biological functions, to
evaluate higher brain function, to study the pathophysiology of various disorders and to develop effective therapy.
Positron emission tomography (PET) and single-photon emission computed tomography are representative
molecular imaging methods. Development of radiopharmaceuticals is a key for successful in vivo imaging. However,
not only the development of new radiopharmaceuticals are important. The quality and behavior of this radiopharmaceuticals
must be improved daily. In this manuscript, the authors introduces experiences and concepts
for development of quality control test to be used in the routine of radiopharmaceuticals industry, in order to verify
the quality of the method and the quality of the final product (radiopharmaceuticals) produced. The results
showed that an alternative methods for quantification of ethanol is required. Despite of the results of the linearity,
the quantification of content of radiopharmaceuticals (F-18 and FDG-18) shown to be satisfactory.
2010-01-01T00:00:00ZRadiofármacos são medicamentos utilizados na Medicina Nuclear para avaliar funções biológicas
e patofisiológicas, realizar estudos do funcionamento do cérebro e diversas outras enfermidades, assim
comodesenvolver uma terapia eficaz. Tomografia por emissão de pósitron e tomografia computadorizada
por emissão de fóton único são alguns dos métodos que utilizam radiofármacos. Desenvolvimento de novos
radiofármacos é a solução para a análise in vivo de imagens endógenas. Contudo não só o desenvolvimento
é importante. A qualidade e o comportamento do radiofármaco desenvolvido devem ser comprovadas ,
diariamente. Nesse trabalho os autores avaliam a metodologia de determinação de etanol e do teor de F-18
e FDG-18 como forma de avaliação da qualidade do produto final, e dos método utilizados, assim como
dos equipamentos. Os resultados revelam que a metodologia de determinação de etanol em solução de
FDG-18 deve ser revista. Contudo a análise de teor de F-18 e FDG-18, mostrou-se bastante eficaz.
Radiopharmaceuticals
are drugs used in Nuclear Medicine to measure physiological and biological functions, to
evaluate higher brain function, to study the pathophysiology of various disorders and to develop effective therapy.
Positron emission tomography (PET) and single-photon emission computed tomography are representative
molecular imaging methods. Development of radiopharmaceuticals is a key for successful in vivo imaging. However,
not only the development of new radiopharmaceuticals are important. The quality and behavior of this radiopharmaceuticals
must be improved daily. In this manuscript, the authors introduces experiences and concepts
for development of quality control test to be used in the routine of radiopharmaceuticals industry, in order to verify
the quality of the method and the quality of the final product (radiopharmaceuticals) produced. The results
showed that an alternative methods for quantification of ethanol is required. Despite of the results of the linearity,
the quantification of content of radiopharmaceuticals (F-18 and FDG-18) shown to be satisfactory.Pulsatile drug delivery for the treatment of nocturnal asthma: a chronopharmaceutical approachMahajan, Ashok N.Pancholi, Shyam S.http://sedici.unlp.edu.ar:80/handle/10915/78872019-06-28T04:02:38Z2010-01-01T00:00:00ZComunicacion
Latin American Journal of Pharmacy; vol. 29, no. 1
The objective of the present study was to develop and evaluate a pulsatile system of theophylline based on chronopharmaceutical considerations. The basic design consists of an impermeable capsule body, which contains the drug and is closed by an erodible tablet plug. The body portion of the hard gelatin capsules was cross-linked by the combined effect of formaldehyde and heat treatment. The formulation variables such as type of plug material, capsule content, different plug composition, plug weight and plug hardness was investigated to characterize the lag time (t10).The results indicated that drug release from the pulsatile capsule exhibited an initial lag period, followed by a stage of rapid drug release. For the complete and rapid drug release from the capsule body, 15% of effervescent agent had to be included in the capsule content. The lag time criterion of 5 hrs was satisfied by the tablet plug containing 16%HPMC K100LV. A good correlation was observed between erodible tablet weight and lag time.
2010-01-01T00:00:00ZThe objective of the present study was to develop and evaluate a pulsatile system of theophylline based on chronopharmaceutical considerations. The basic design consists of an impermeable capsule body, which contains the drug and is closed by an erodible tablet plug. The body portion of the hard gelatin capsules was cross-linked by the combined effect of formaldehyde and heat treatment. The formulation variables such as type of plug material, capsule content, different plug composition, plug weight and plug hardness was investigated to characterize the lag time (t10).The results indicated that drug release from the pulsatile capsule exhibited an initial lag period, followed by a stage of rapid drug release. For the complete and rapid drug release from the capsule body, 15% of effervescent agent had to be included in the capsule content. The lag time criterion of 5 hrs was satisfied by the tablet plug containing 16%HPMC K100LV. A good correlation was observed between erodible tablet weight and lag time.Antimutagenic effect of Phyllanthus orbicularis against γ-radiationAlonso, AlenaFuentes, Jorge L.Sánchez Lamar, AngelLlagostera, Montserrathttp://sedici.unlp.edu.ar:80/handle/10915/78862019-06-28T04:02:37Z2010-01-01T00:00:00ZComunicacion
Latin American Journal of Pharmacy; vol. 29, no. 1
The present work evaluated the protective effect of aqueous extract of Phyllanthus orbicularis against the mutagenicity of γ-radiation. The extract activity was determined in pre- and post-treatment procedures using Salmonella reversion assay. In either case, the plant extract protected bacterial cells against the mutations induced by γ-radiation, suggesting that it contains antimutagenic compounds which confer protection by at least two different mechanisms: i) as antioxidants targeting the oxidative mutagens induced by γ-radiation and ii) by modulation of the cellular repair enzymes acting on damaged DNA. The results are discussed in relation to the chemopreventive and radioprotective potential of P. orbicularis.
2010-01-01T00:00:00ZThe present work evaluated the protective effect of aqueous extract of Phyllanthus orbicularis against the mutagenicity of γ-radiation. The extract activity was determined in pre- and post-treatment procedures using Salmonella reversion assay. In either case, the plant extract protected bacterial cells against the mutations induced by γ-radiation, suggesting that it contains antimutagenic compounds which confer protection by at least two different mechanisms: i) as antioxidants targeting the oxidative mutagens induced by γ-radiation and ii) by modulation of the cellular repair enzymes acting on damaged DNA. The results are discussed in relation to the chemopreventive and radioprotective potential of P. orbicularis.Comparison between UV spectrophotometric and capillary electrophoresis methods for determination of rabeprazole sodium in pharmaceutical formulationsGarcia, Cássia V.Mendez, Andreas L.Steppe, MartinSchapoval, Elfrides E. S.http://sedici.unlp.edu.ar:80/handle/10915/78852019-06-28T04:02:36Z2010-01-01T00:00:00ZComunicacion
Latin American Journal of Pharmacy; vol. 29, no. 1
Rabeprazole sodium (RAB) is an anti-secretory agent which inhibits the enzyme H<sup>+</sup> /K<sup>+</sup> ATPase, present in the stomach parietal cells. The aim of this work is to develop and validate a simple and fast ultraviolet spectrophotometric method (UV) for quantification of RAB in pharmaceutical formulation and compare it with a capillary electrophoresis (CE) one, previously validated. The UV technique was applied using water (pH 10.0) as diluent and the determinations were made at λ = 291 nm. The method showed good linearity (r = 0.9997) in the concentration range of 6.0 to 18.0 μg ml<sup>-1</sup> .(The intra- and interday precision data demonstrated the method has good repeatability (RSD = 0.52 and 0.82, respectively . Accuracy and specificity were also evaluated and results were satisfactory. The detection and quantitation limits were 0.32 and 0.95 μg ml<sup>-1</sup> , respectively. Both methods demonstrated to be adequate for the intended purpose.
2010-01-01T00:00:00ZRabeprazole sodium (RAB) is an anti-secretory agent which inhibits the enzyme H<sup>+</sup> /K<sup>+</sup> ATPase, present in the stomach parietal cells. The aim of this work is to develop and validate a simple and fast ultraviolet spectrophotometric method (UV) for quantification of RAB in pharmaceutical formulation and compare it with a capillary electrophoresis (CE) one, previously validated. The UV technique was applied using water (pH 10.0) as diluent and the determinations were made at λ = 291 nm. The method showed good linearity (r = 0.9997) in the concentration range of 6.0 to 18.0 μg ml<sup>-1</sup> .(The intra- and interday precision data demonstrated the method has good repeatability (RSD = 0.52 and 0.82, respectively . Accuracy and specificity were also evaluated and results were satisfactory. The detection and quantitation limits were 0.32 and 0.95 μg ml<sup>-1</sup> , respectively. Both methods demonstrated to be adequate for the intended purpose.An orientin derivative isolated from Passiflora tripartita var. mollissimaRamos, Freddy A.Castellanos, LeonardoLópez, CésarPalacios, LizethDuque, CarmenzaPacheco, RicardoGuzmán, Antoniohttp://sedici.unlp.edu.ar:80/handle/10915/78842013-10-17T12:02:29Z2010-01-01T00:00:00ZComunicacion
Latin American Journal of Pharmacy; vol. 29, no. 1
Passiflora tripartita var. mollisima (banana passion fruit) is an edible fruit widespread in the Andean highlands of Colombia and Ecuador. The fruit is used for juices as well as for the sedative properties of the leaves. As a contribution to the chemical characterization of this species, a new compound, 4'- methoxyluteolin-8-C-6”acetylglucopyranoside, was isolated from the ethanolic extract of Passiflora tripartita var. mollisima leaves and identified by spectroscopical data (NMR, MS, UV).
2010-01-01T00:00:00ZPassiflora tripartita var. mollisima (banana passion fruit) is an edible fruit widespread in the Andean highlands of Colombia and Ecuador. The fruit is used for juices as well as for the sedative properties of the leaves. As a contribution to the chemical characterization of this species, a new compound, 4'- methoxyluteolin-8-C-6”acetylglucopyranoside, was isolated from the ethanolic extract of Passiflora tripartita var. mollisima leaves and identified by spectroscopical data (NMR, MS, UV).Effects of rutin from leaves and flowers of buckwheat (Fagopyrum esculentum Moench.) on angiotensin II-induced hypertrophy of cardiac myocytes and proliferation of fibroblastsHan, Shu-YingChu, Jin-XiuLi, Guang-MinZhu, Li-ShaShi, Rui-Fanghttp://sedici.unlp.edu.ar:80/handle/10915/78832019-06-28T04:02:35Z2010-01-01T00:00:00ZComunicacion
Latin American Journal of Pharmacy; vol. 29, no. 1
Rutin was isolated from dried leaves and flowers of buckwheat (Fagopyrum esculentum
Moench.). The effects of rutin on angiotensin II-induced hypertrophy of cultured cardiac myocytes and
proliferation of cardiac fibroblasts of neonatal rats were evaluated by analyzing the cell surface area, measuring
the protein synthesis rate through 3H-leucine incorporation, and the MTT method. Rutin (0.8 to 8.0
mg/l) exhibited a strong inhibition on the hypertrophy and proliferation. The results suggested that rutin
from buckwheat flowers and leaves might have protective effects on cardiac hypertrophy.
2010-01-01T00:00:00ZRutin was isolated from dried leaves and flowers of buckwheat (Fagopyrum esculentum
Moench.). The effects of rutin on angiotensin II-induced hypertrophy of cultured cardiac myocytes and
proliferation of cardiac fibroblasts of neonatal rats were evaluated by analyzing the cell surface area, measuring
the protein synthesis rate through 3H-leucine incorporation, and the MTT method. Rutin (0.8 to 8.0
mg/l) exhibited a strong inhibition on the hypertrophy and proliferation. The results suggested that rutin
from buckwheat flowers and leaves might have protective effects on cardiac hypertrophy.Prácticas de prevención y tratamiento de la pediculosis capitis en Comodoro Rivadavia, ArgentinaDelgado, Adriana G. C.Kurdelas, RitaGamarra, KarinaArtola, Silvina B.Guerreiro, Mónica das NevesMaure, AdrianaSilva, Christian F.Souto, Mónica G.Flores, María E.Martínez, Rubén D.http://sedici.unlp.edu.ar:80/handle/10915/78822019-06-28T04:02:34Z2010-01-01T00:00:00ZComunicacion
Prevention and treatment practices of pediculosis capitis in Comodoro Rivadavia, Argentina
Latin American Journal of Pharmacy; vol. 29, no. 1
Para evaluar los procedimientos y las sustancias que la población de Comodoro Rivadavia emplea contra la pediculosis en escolares, se analizaron los resultados de 4951 encuestas realizadas entre 2006 y 2007. El 95% de los encuestados revisa periódicamente la cabeza de los niños, lo que resulta favorable para el control de la afección. El 56,4% emplea procedimientos o productos preventivos adecuados, mientras el resto utiliza otros de escasa efectividad preventiva (inocuos o peligrosos). Un 38,11% utiliza tratamientos de acción comprobada. Se constató el uso de productos no indicados para la pediculosis o inefectivos, algunos inocuos y otros con potenciales efectos indeseados. En general, se observa poco discernimiento entre prevención y tratamiento. Se evidenciaron prácticas y creencias erróneas, tales como la vinculación de la pediculosis con falta de higiene, el uso preventivo de pediculicidas, así como el empleo de productos inefectivos para tratar pediculosis activa: entre otros jabón blanco, vinagre, repelentes y desinfectantes e insecticidas de uso veterinario y doméstico.; In order to analyze products and procedures that Comodoro Rivadavia population uses against head lice in school children, results from 4951 surveys obtained in 2006 and 2007 are presented. It is advantageous that 95% of the parents check frequently over the children scalps. About 56,4% uses appropriate preventive products or procedures, while the others use ineffective (innocuous or dangerous) substances. A 38,11% of the population uses appropriate treatment products. The use of products which are not indicated in pediculosis treatment or are ineffective against the infection (innocuous or unsafe substances) was evidenced. Generally, there is scarce differentiation between prevention and treatment. Additionally, erroneous believes and practices were detected: association pediculosis - poor hygiene practices, preventive use of pediculicides, as well as use of ineffective treatment products: soap, vinegar, repellents, and the use of veterinary or domestic disinfectants and insecticides.
2010-01-01T00:00:00ZPara evaluar los procedimientos y las sustancias que la población de Comodoro Rivadavia emplea contra la pediculosis en escolares, se analizaron los resultados de 4951 encuestas realizadas entre 2006 y 2007. El 95% de los encuestados revisa periódicamente la cabeza de los niños, lo que resulta favorable para el control de la afección. El 56,4% emplea procedimientos o productos preventivos adecuados, mientras el resto utiliza otros de escasa efectividad preventiva (inocuos o peligrosos). Un 38,11% utiliza tratamientos de acción comprobada. Se constató el uso de productos no indicados para la pediculosis o inefectivos, algunos inocuos y otros con potenciales efectos indeseados. En general, se observa poco discernimiento entre prevención y tratamiento. Se evidenciaron prácticas y creencias erróneas, tales como la vinculación de la pediculosis con falta de higiene, el uso preventivo de pediculicidas, así como el empleo de productos inefectivos para tratar pediculosis activa: entre otros jabón blanco, vinagre, repelentes y desinfectantes e insecticidas de uso veterinario y doméstico.
In order to analyze products and procedures that Comodoro Rivadavia population uses against head lice in school children, results from 4951 surveys obtained in 2006 and 2007 are presented. It is advantageous that 95% of the parents check frequently over the children scalps. About 56,4% uses appropriate preventive products or procedures, while the others use ineffective (innocuous or dangerous) substances. A 38,11% of the population uses appropriate treatment products. The use of products which are not indicated in pediculosis treatment or are ineffective against the infection (innocuous or unsafe substances) was evidenced. Generally, there is scarce differentiation between prevention and treatment. Additionally, erroneous believes and practices were detected: association pediculosis - poor hygiene practices, preventive use of pediculicides, as well as use of ineffective treatment products: soap, vinegar, repellents, and the use of veterinary or domestic disinfectants and insecticides.Estado nutricional e uso de medicamentos por idososFaria, Marina Q.Franceschini, Sylvia C.C.Ribeiro, Andréiahttp://sedici.unlp.edu.ar:80/handle/10915/78812019-06-28T04:02:32Z2010-01-01T00:00:00ZComunicacion
Nutritional status and use of drugs by elders
Latin American Journal of Pharmacy; vol. 29, no. 1
O aumento da freqüência das doenças crônico-degenerativas associadas ao envelhecimento implica, muitas vezes, no uso contínuo de fármacos. Paralelamente, os idosos são mais propensos a alterações nutricionais, devido a distintos fatores. De acordo com a literatura, as alterações nutricionais com influência potencial no efeito de um maior número de fármacos são as mudanças na concentração de proteínas plasmáticas -hipoalbuminemia e alterações na α-glicoproteína ácida e na composição corporal. Tais alterações podem modificar os efeitos de agentes sedativos, cardiovasculares, diuréticos, entre outros. A consideração desses aspectos é fundamental para melhorar o uso de medicamentos entre idosos.; The frequency increase of chronic degenerative diseases associated with elderly usually implies the continuous use of medicines. Elders are more prone to nutritional changes as a function of different factors. According to the literature, the nutritional changes with potential influence in the effect of most drugs are the modification in the concentration of plasmatic proteins -hypoalbuminemia and acid α-1-glycoprotein-, and in the body composition. Such alterations may modify the effects of sedative, cardiovascular agents, anticonvulsives, and diuretics, among others. Consideration of these aspects is a basic condition to improve the use of medicines among elderly people.
2010-01-01T00:00:00ZO aumento da freqüência das doenças crônico-degenerativas associadas ao envelhecimento implica, muitas vezes, no uso contínuo de fármacos. Paralelamente, os idosos são mais propensos a alterações nutricionais, devido a distintos fatores. De acordo com a literatura, as alterações nutricionais com influência potencial no efeito de um maior número de fármacos são as mudanças na concentração de proteínas plasmáticas -hipoalbuminemia e alterações na α-glicoproteína ácida e na composição corporal. Tais alterações podem modificar os efeitos de agentes sedativos, cardiovasculares, diuréticos, entre outros. A consideração desses aspectos é fundamental para melhorar o uso de medicamentos entre idosos.
The frequency increase of chronic degenerative diseases associated with elderly usually implies the continuous use of medicines. Elders are more prone to nutritional changes as a function of different factors. According to the literature, the nutritional changes with potential influence in the effect of most drugs are the modification in the concentration of plasmatic proteins -hypoalbuminemia and acid α-1-glycoprotein-, and in the body composition. Such alterations may modify the effects of sedative, cardiovascular agents, anticonvulsives, and diuretics, among others. Consideration of these aspects is a basic condition to improve the use of medicines among elderly people.Mutagenicactivity of glycoallkaloids from Solanum palinacanthum Dunal (Solanaceae) found in the brazilian cerradoAlmeida, Adelia E.Cardoso, Cássia R.P.Almeida, Daniela V.Moreira, Raquel R.D.Silva, MárciaVaranda, Eliana A.http://sedici.unlp.edu.ar:80/handle/10915/78802019-06-27T20:04:43Z2010-01-01T00:00:00ZComunicacion
Latin American Journal of Pharmacy; vol. 29, no. 1
Solanaceous plants are widely distributed around the world and they are traditionally used as drugs for the treatment of cancer and herpes, and include familiar foods such as potato, tomato and eggplant and some berries popular in Brazil. As part of a program of research on pharmacologically active new molecules, the aim in this study was to assess the mutagenic effects of Solanum palinacanthum, known popularly as joá. The crude 95% ethanol extract and purified solamargine obtained from the fruits of S. palinacanthum Dunal were investigated by the Ames test, using the Salmonella typhimurium strains TA98, TA97a, TA100 and TA102 as test organisms, with and without metabolic activation. The concentrations tested ranged from 0.07 to 15.0 mg/plate for the crude ethanolic extract and from 1.25 to 5.0 mg/plate for the solamargine. The results showed a mutagenic effect of both the extract and the solamargine in the TA98 strain (without metabolic activation). The present study showed the potential mutagenicity and suggests confirming this effect in other models, before recommending their indiscriminate consumption by the population.
2010-01-01T00:00:00ZSolanaceous plants are widely distributed around the world and they are traditionally used as drugs for the treatment of cancer and herpes, and include familiar foods such as potato, tomato and eggplant and some berries popular in Brazil. As part of a program of research on pharmacologically active new molecules, the aim in this study was to assess the mutagenic effects of Solanum palinacanthum, known popularly as joá. The crude 95% ethanol extract and purified solamargine obtained from the fruits of S. palinacanthum Dunal were investigated by the Ames test, using the Salmonella typhimurium strains TA98, TA97a, TA100 and TA102 as test organisms, with and without metabolic activation. The concentrations tested ranged from 0.07 to 15.0 mg/plate for the crude ethanolic extract and from 1.25 to 5.0 mg/plate for the solamargine. The results showed a mutagenic effect of both the extract and the solamargine in the TA98 strain (without metabolic activation). The present study showed the potential mutagenicity and suggests confirming this effect in other models, before recommending their indiscriminate consumption by the population.Composition and biological activity of tea polysaccharides obtained by water extraction and enzymatic extractionWei, XinlinYang, ZhiweiGuo, YanhongXiao, JianboWang, Yuanfenghttp://sedici.unlp.edu.ar:80/handle/10915/78792019-06-27T20:04:42Z2010-01-01T00:00:00ZComunicacion
Latin American Journal of Pharmacy; vol. 29, no. 1
The composition and biological activities of tea polysaccharides (TPS) obtained by traditional water extraction, boiling water extraction and enzymatic extraction were investigated. Boiling water extraction (100 °C for 2 h) was found to be the optimal method with higher yield of TPS (1.91%) consisting of higher contents of neutral saccharides (57.82%) and acid saccharides (26.95%) with lowest protein content (3.06%). TPS obtained by boiling water extraction exhibited a strong inhibitory effect on α-glucosidase with the inhibitory rate of 86.67%. The inhibitory effect of TPS on α-glucosidase increased with increasing neutral polysaccharides content in TPS. TPS obtained by boiling water extraction (50 μg/mL) had very strong proliferation effect on lymphocyte.
2010-01-01T00:00:00ZThe composition and biological activities of tea polysaccharides (TPS) obtained by traditional water extraction, boiling water extraction and enzymatic extraction were investigated. Boiling water extraction (100 °C for 2 h) was found to be the optimal method with higher yield of TPS (1.91%) consisting of higher contents of neutral saccharides (57.82%) and acid saccharides (26.95%) with lowest protein content (3.06%). TPS obtained by boiling water extraction exhibited a strong inhibitory effect on α-glucosidase with the inhibitory rate of 86.67%. The inhibitory effect of TPS on α-glucosidase increased with increasing neutral polysaccharides content in TPS. TPS obtained by boiling water extraction (50 μg/mL) had very strong proliferation effect on lymphocyte.Technological development of hard capsules of sertraline hydrochlorideIzquierdo, Adalberto C.Fernández C., MirnaCastellanos Gil, Eddyhttp://sedici.unlp.edu.ar:80/handle/10915/78782019-06-27T20:04:41Z2010-01-01T00:00:00ZArticulo
Latin American Journal of Pharmacy; vol. 29, no. 1
Fast release capsules, containing sertraline hydrochloride, pregelatinized maize starch and
microcrystalline cellulose were formulated. For this purpose, different technological assays were elaborated
being the formulation 2 selected as the better technological variant. Dry powders were filled into hard
gelatin capsules. From this formulation were derived capsules with satisfactory technological properties.
The quantification of sertraline through HPLC UV detection method was validated for accuracy, precision,
linearity and selectivity. The method was linear over the concentration range 0.5 to 0.75 mg/mL and
was shown to be highly reproducible. It could be used, without any interference of capsule excipients, for
determination of sertraline from solid dosage form. Hard capsules showed an adequate stability during 24
months demonstrating the feasibility from the process of production of this formulation. Parameters, f1
and f2, were used to confirm similarity of dissolution, in deaerated distilled water, of test formulation and
capsules of Prosertin as reference product.
2010-01-01T00:00:00ZFast release capsules, containing sertraline hydrochloride, pregelatinized maize starch and
microcrystalline cellulose were formulated. For this purpose, different technological assays were elaborated
being the formulation 2 selected as the better technological variant. Dry powders were filled into hard
gelatin capsules. From this formulation were derived capsules with satisfactory technological properties.
The quantification of sertraline through HPLC UV detection method was validated for accuracy, precision,
linearity and selectivity. The method was linear over the concentration range 0.5 to 0.75 mg/mL and
was shown to be highly reproducible. It could be used, without any interference of capsule excipients, for
determination of sertraline from solid dosage form. Hard capsules showed an adequate stability during 24
months demonstrating the feasibility from the process of production of this formulation. Parameters, f1
and f2, were used to confirm similarity of dissolution, in deaerated distilled water, of test formulation and
capsules of Prosertin as reference product.Estandarización y caracterización organoléptica y físico-química de 15 propóleos argentinosLozina, LauraPeichoto, Maria E.Acosta, OfeliaGranero, Gladys E.http://sedici.unlp.edu.ar:80/handle/10915/78772019-06-27T20:04:41Z2010-01-01T00:00:00ZArticulo
Standardization and organoleptic and physicochemical characterization of 15 argentinean propolis
Latin American Journal of Pharmacy; vol. 29, no. 1
El propóleos es una mezcla de composición química compleja que posee propiedades biológicas de gran interés para fines terapéuticos. Existen distintos tipos de propóleos que difieren en su composición química de acuerdo a su origen botánico. En la actualidad se carece de un estándar de calidad de propóleos. Por esta razón, en este trabajo fueron determinados los caracteres organolépticos, propiedades físico- químicas y el contenido total de compuestos fenólicos y flavonoides de 15 muestras de propóleos recolectadas de diferentes regiones de Chaco, Corrientes, Santiago del Estero y Mendoza, para así brindar una estandarización que permita producir medicamentos de propóleos de seguridad y eficacia demostrable. Se encontró gran variación en las propiedades de los diferentes propóleos estudiados, incluso de aquellos provenientes de una misma región. Los propóleos difirieron principalmente en su contenido de compuestos fenólicos y flavonoides, siendo significativamente superiores en los propóleos originarios de Mendoza, donde la vegetación más abundante pertenece a Populus sp.; Propolis is a mixture of complex chemical composition that possesses biological properties of great interest for therapeutic purposes. Different types of propolis exist, which differ in its chemical composition according to its botanical origin. At present, there is not a quality standard for propolis. For this reason, in this work the organoleptic characteristics, the physicochemical properties, the total content of phenolic compounds and the flavonoid composition of 15 samples of propolis collected from different regions of Chaco, Corrientes, Santiago del Estero, and Mendoza were determined, for providing an standardization that allows the production of effective and safe propolis products. It was found great variation in the properties of the different studied propolis, even of those from the same region. Principally, propolis differed in its phenolic and flavonoids content, being significantly higher in propolis from Mendoza, where the most abundant vegetation belongs to Populus sp.
2010-01-01T00:00:00ZEl propóleos es una mezcla de composición química compleja que posee propiedades biológicas de gran interés para fines terapéuticos. Existen distintos tipos de propóleos que difieren en su composición química de acuerdo a su origen botánico. En la actualidad se carece de un estándar de calidad de propóleos. Por esta razón, en este trabajo fueron determinados los caracteres organolépticos, propiedades físico- químicas y el contenido total de compuestos fenólicos y flavonoides de 15 muestras de propóleos recolectadas de diferentes regiones de Chaco, Corrientes, Santiago del Estero y Mendoza, para así brindar una estandarización que permita producir medicamentos de propóleos de seguridad y eficacia demostrable. Se encontró gran variación en las propiedades de los diferentes propóleos estudiados, incluso de aquellos provenientes de una misma región. Los propóleos difirieron principalmente en su contenido de compuestos fenólicos y flavonoides, siendo significativamente superiores en los propóleos originarios de Mendoza, donde la vegetación más abundante pertenece a Populus sp.
Propolis is a mixture of complex chemical composition that possesses biological properties of great interest for therapeutic purposes. Different types of propolis exist, which differ in its chemical composition according to its botanical origin. At present, there is not a quality standard for propolis. For this reason, in this work the organoleptic characteristics, the physicochemical properties, the total content of phenolic compounds and the flavonoid composition of 15 samples of propolis collected from different regions of Chaco, Corrientes, Santiago del Estero, and Mendoza were determined, for providing an standardization that allows the production of effective and safe propolis products. It was found great variation in the properties of the different studied propolis, even of those from the same region. Principally, propolis differed in its phenolic and flavonoids content, being significantly higher in propolis from Mendoza, where the most abundant vegetation belongs to Populus sp.Estudo anatômico e prospecção fitoquímica de folhas de Eucalyptus benthamii Maiden et CambageDöll, Patricia M.Farago, Paulo V.Nakashima, TomoeSantos, Paulo E. T. dosPaula, Josiane de F.P. dehttp://sedici.unlp.edu.ar:80/handle/10915/78762019-06-27T20:04:38Z2010-01-01T00:00:00ZArticulo
Anatomical study and phytochemical screening of leaves from Eucalyptus benthamii Maiden et Cambage
Latin American Journal of Pharmacy; vol. 29, no. 1
O óleo essencial das folhas de Eucalyptus benthamii Maiden et Cambage, espécie de elevada resistência ao frio, contém α-pineno como constituinte majoritário. Este trabalho avaliou a anatomia foliar de E. benthamii e realizou um screening fitoquímico para investigar a presença de outros metabólitos secundários nas folhas desse táxon. A partir do material botânico fixado, foram conduzidas as análises anatômicas, utilizando os métodos usuais de microscopia fotônica e eletrônica. O screening fitoquímico foi desenvolvido por meio de reações específicas para triterpenos/esteróides, alcalóides, antraquinonas, cumarinas, flavonóides, saponinas e taninos. A anatomia foliar evidenciou estômatos anomocíticos em ambas as faces do limbo foliar, mesofilo heterogêneo simétrico isobilateral, nervura mediana plano-convexa e sistema vascular do tipo bicolateral em arco aberto. Também foram visualizados idioblastos contendo cristais de oxalato de cálcio, cavidades secretoras com conteúdo lipofílico e súber cicatricial. O estudo fitoquímico revelou a presença de triterpenos e/ou esteróides, glicosídeos flavônicos, glicosídeos saponínicos e taninos.; Eucalyptus benthamii Maiden et Cambage is a species with remarkable cold tolerance. Its essential oil showed α-pinene as the major constituent. The aim of this paper was to evaluate the anatomy of leaves from E. benthamii. Also a phytochemical screening of others secondary metabolites was investigated. The anatomical analyses of previously fixed leaves from E. benthamii were performed by the usual light and scanning microtechniques. Phytochemical study was carried out by qualitative reactions for triterpenes/steroids, alkaloids, anthraquinones, coumarins, flavonoids, saponins and tannins. Anomocytic stomata were observed on both surfaces of leaves from E. benthamii. Foliar blade showed a heterogeneous and symmetrical mesophyll as isobilateral arrange. The midrib showed plain-convex cross-section and was traversed by a bicollateral vascular bundle in open arc. Idioblasts with calcium oxalate prisms, secretory cavities with oil content and lenticel-like structures were also reported. The phytochemical screening revealed triterpenes/steroids, flavonoid glycosides, saponin glycosides and tannins.
2010-01-01T00:00:00ZO óleo essencial das folhas de Eucalyptus benthamii Maiden et Cambage, espécie de elevada resistência ao frio, contém α-pineno como constituinte majoritário. Este trabalho avaliou a anatomia foliar de E. benthamii e realizou um screening fitoquímico para investigar a presença de outros metabólitos secundários nas folhas desse táxon. A partir do material botânico fixado, foram conduzidas as análises anatômicas, utilizando os métodos usuais de microscopia fotônica e eletrônica. O screening fitoquímico foi desenvolvido por meio de reações específicas para triterpenos/esteróides, alcalóides, antraquinonas, cumarinas, flavonóides, saponinas e taninos. A anatomia foliar evidenciou estômatos anomocíticos em ambas as faces do limbo foliar, mesofilo heterogêneo simétrico isobilateral, nervura mediana plano-convexa e sistema vascular do tipo bicolateral em arco aberto. Também foram visualizados idioblastos contendo cristais de oxalato de cálcio, cavidades secretoras com conteúdo lipofílico e súber cicatricial. O estudo fitoquímico revelou a presença de triterpenos e/ou esteróides, glicosídeos flavônicos, glicosídeos saponínicos e taninos.
Eucalyptus benthamii Maiden et Cambage is a species with remarkable cold tolerance. Its essential oil showed α-pinene as the major constituent. The aim of this paper was to evaluate the anatomy of leaves from E. benthamii. Also a phytochemical screening of others secondary metabolites was investigated. The anatomical analyses of previously fixed leaves from E. benthamii were performed by the usual light and scanning microtechniques. Phytochemical study was carried out by qualitative reactions for triterpenes/steroids, alkaloids, anthraquinones, coumarins, flavonoids, saponins and tannins. Anomocytic stomata were observed on both surfaces of leaves from E. benthamii. Foliar blade showed a heterogeneous and symmetrical mesophyll as isobilateral arrange. The midrib showed plain-convex cross-section and was traversed by a bicollateral vascular bundle in open arc. Idioblasts with calcium oxalate prisms, secretory cavities with oil content and lenticel-like structures were also reported. The phytochemical screening revealed triterpenes/steroids, flavonoid glycosides, saponin glycosides and tannins.Utilización de medicamentos en un programa de atención primaria de salud para poblaciones rurales marginalesGualda, Marcelo A.Carignano, Luciana S.Páez, Paulina L.Bezzi, GiselaParaje, María Gabrielahttp://sedici.unlp.edu.ar:80/handle/10915/78752019-06-27T20:04:37Z2010-01-01T00:00:00ZArticulo
Utilization of medicines in a program of primary health care in rural- marginal populations
Latin American Journal of Pharmacy; vol. 29, no. 1
Se determinaron indicadores básicos del uso de medicamentos en Atención Primaria de Salud (APS) en un programa solidario y se implementaron estrategias de Atención Farmacéutica para mejorar la calidad de la dispensación y la educación sanitaria, asegurando el suministro de medicamentos esenciales a una población rural-marginal de Argentina. Se analizaron las prescripciones médicas siguiendo parámetros de la Organización Mundial de la Salud (OMS) sobre el uso de medicamentos, mediante la determinación de tres indicadores básicos: las prácticas de prescripción, la asistencia al paciente y los factores específicos del servicio de Farmacia. El lugar de realización fue la zona rural de Cachi Yaco (Córdoba). La utilización de los indicadores básicos OMS fue una herramienta simple que permitió evaluar con rapidez y seguridad aspectos importantes de la APS. El trabajo coordinado y comprometido de los distintos profesionales del equipo de salud, alumnos y voluntarios, permitió satisfacer en un alto porcentaje el acceso a medicamentos esta comunidad.; The aim of this work was to determine the basic indicators of medicines in Primary Health Care (PHC) in a social program, and to implement the strategies of Pharmaceutical Care in order to improve the quality of the dispensation and sanitary education, thus assuring the supply of essential medicines in rural-marginal populations. The method chosen was to apply the parameters of the World Health Organization (WHO) to determine three basic indicators: the practice of prescription, the assistance to the patient and the specific factors of the pharmaceutical service. The place of the study was Cachi Yaco in the province of Córdoba, Argentina. The utilization of the basic indicators of the WHO were a simple tool that allowed us to evaluate quickly important safety aspects of the pharmaceutical practice in the field of the PHC. Using coordinated work of the professionals, students and volunteers, the access to medicines in this community was realized in an efficient way.
2010-01-01T00:00:00ZSe determinaron indicadores básicos del uso de medicamentos en Atención Primaria de Salud (APS) en un programa solidario y se implementaron estrategias de Atención Farmacéutica para mejorar la calidad de la dispensación y la educación sanitaria, asegurando el suministro de medicamentos esenciales a una población rural-marginal de Argentina. Se analizaron las prescripciones médicas siguiendo parámetros de la Organización Mundial de la Salud (OMS) sobre el uso de medicamentos, mediante la determinación de tres indicadores básicos: las prácticas de prescripción, la asistencia al paciente y los factores específicos del servicio de Farmacia. El lugar de realización fue la zona rural de Cachi Yaco (Córdoba). La utilización de los indicadores básicos OMS fue una herramienta simple que permitió evaluar con rapidez y seguridad aspectos importantes de la APS. El trabajo coordinado y comprometido de los distintos profesionales del equipo de salud, alumnos y voluntarios, permitió satisfacer en un alto porcentaje el acceso a medicamentos esta comunidad.
The aim of this work was to determine the basic indicators of medicines in Primary Health Care (PHC) in a social program, and to implement the strategies of Pharmaceutical Care in order to improve the quality of the dispensation and sanitary education, thus assuring the supply of essential medicines in rural-marginal populations. The method chosen was to apply the parameters of the World Health Organization (WHO) to determine three basic indicators: the practice of prescription, the assistance to the patient and the specific factors of the pharmaceutical service. The place of the study was Cachi Yaco in the province of Córdoba, Argentina. The utilization of the basic indicators of the WHO were a simple tool that allowed us to evaluate quickly important safety aspects of the pharmaceutical practice in the field of the PHC. Using coordinated work of the professionals, students and volunteers, the access to medicines in this community was realized in an efficient way.Sedative and cardiovascular effects of <i>Aloysia citriodora</i> Palau, on mice and ratsRagone, María InésSella, MarianaPastore, AgustínConsolini, Alicia E.http://sedici.unlp.edu.ar:80/handle/10915/78742019-06-27T20:04:35Z2010-01-01T00:00:00ZArticulo
Latin American Journal of Pharmacy; vol. 29, no. 1
<i>Aloysia citriodora</i> Palau, Verbenaceae ("cedrón") is widely used as infusion or decoction in South America to treat indigestion, tachycardia and anxiety. We previously demonstrated its antispasmodic effect on rat duodenum. Now, its aqueous extract (AEC) from 1 to 10 mg/kg was sedative in mice on the open-field, effect which was potentiated by diazepam and sensitive to flumazenil. In normotensive rats, 1 to 30 mg AEC/kg induced a transitory hypotension, insensitive to atropine and L-NAME. Regarding an effect on α-adrenergic receptors, AEC non-competitively blocked the phenylephrine contraction on vas deferens. In isolated rat hearts, AEC induced negative inotropism, as well as vitexin, the main component. Then, the benzodiazepine-like sedation, negative inotropism and antispasmodic effect preclinically justify its popular use for abdominal cramps and as coadjuvant for anxiety and angor.
2010-01-01T00:00:00Z<i>Aloysia citriodora</i> Palau, Verbenaceae ("cedrón") is widely used as infusion or decoction in South America to treat indigestion, tachycardia and anxiety. We previously demonstrated its antispasmodic effect on rat duodenum. Now, its aqueous extract (AEC) from 1 to 10 mg/kg was sedative in mice on the open-field, effect which was potentiated by diazepam and sensitive to flumazenil. In normotensive rats, 1 to 30 mg AEC/kg induced a transitory hypotension, insensitive to atropine and L-NAME. Regarding an effect on α-adrenergic receptors, AEC non-competitively blocked the phenylephrine contraction on vas deferens. In isolated rat hearts, AEC induced negative inotropism, as well as vitexin, the main component. Then, the benzodiazepine-like sedation, negative inotropism and antispasmodic effect preclinically justify its popular use for abdominal cramps and as coadjuvant for anxiety and angor.Validação de método de cromatografia líquida de alta eficiência para análise de ampicilina em injetáveis utilizados em hospitais públicos de Recife, BrasilFarias, Tatianne A. L.Filho, José A. R.Francelino, LúciaOliveira, Eduardo José Alécio deSilva, Ronaldy J.M.C.L.http://sedici.unlp.edu.ar:80/handle/10915/78732019-06-27T20:04:34Z2010-01-01T00:00:00ZArticulo
Validation of a high-performance liquid chromatography method for determination of injectable sodium ampicillin used at public hospitals in Recife, Brasil
Latin American Journal of Pharmacy; vol. 29, no. 1
Um método de cromatografia líquida de alta eficiência mais econômico devido ao uso de um padrão secundário de ampicilina tri-hidratada foi validado e utilizado para a quantificação de ampicilina sódica injetável em amostras coletadas em seis hospitais públicos da cidade de Recife-PE. A fase móvel foi ajustada com fosfato de potássio monobásico 1M pH 3,0, de forma que a separação dos picos ocorresse em tempo inferior a 12 minutos. As análises foram realizadas em coluna ODS Shim-pack de 250 mm x 4,6 mm, partículas de 5 μm, em fluxo de 1,5 mL/min e detecção a 254 nm. O método apresentou especificidade, linearidade, exatidão e robustez. Os lotes de ampicilina sódica injetável analisados apresentaram faixa de ampicilina sódica de 96% a 114% da concentração de análise (1,0 mg/mL), atendendo ao especificado na Farmacopéia Brasileira que estabelece um intervalo de aceitação de 90 a 115%.
2010-01-01T00:00:00ZUm método de cromatografia líquida de alta eficiência mais econômico devido ao uso de um padrão secundário de ampicilina tri-hidratada foi validado e utilizado para a quantificação de ampicilina sódica injetável em amostras coletadas em seis hospitais públicos da cidade de Recife-PE. A fase móvel foi ajustada com fosfato de potássio monobásico 1M pH 3,0, de forma que a separação dos picos ocorresse em tempo inferior a 12 minutos. As análises foram realizadas em coluna ODS Shim-pack de 250 mm x 4,6 mm, partículas de 5 μm, em fluxo de 1,5 mL/min e detecção a 254 nm. O método apresentou especificidade, linearidade, exatidão e robustez. Os lotes de ampicilina sódica injetável analisados apresentaram faixa de ampicilina sódica de 96% a 114% da concentração de análise (1,0 mg/mL), atendendo ao especificado na Farmacopéia Brasileira que estabelece um intervalo de aceitação de 90 a 115%.Avaliação da atividade farmacológica de Pfaffia paniculata (Martius) KuntzeFontanive, Tiago O.Kobayashi, CristineBona, Laura R.Massoni, TalitaWeizenmann, MarinaTasca, TianaGamaro, Giovana D.Maluf, Rage W.Picoli, Simone U.Ardenghi, PatríciaSuyenaga, Edna S.http://sedici.unlp.edu.ar:80/handle/10915/78722019-06-27T20:04:34Z2010-01-01T00:00:00ZArticulo
Evaluation of the pharmacological activity of Pfaffia paniculata (Martius) Kuntze
Latin American Journal of Pharmacy; vol. 29, no. 1
O presente trabalho teve como objetivo investigar o efeito anti-inflamatório, antimicrobiano, antiprotozoário e possível ação sobre o sistema nervoso central (SNC) em ratos tratados com extrato hidroalcoólico de Pfaffia paniculata. Verificou-se atividade anti-inflamatória tanto in vivo, na dose de 100 mg/kg, como in vitro nas concentrações de 50 e 100 μg/mL. Porém, verificou-se efeito pró-inflamatório na dose de 200 mg/kg, pelo ensaio de pleurisia e de 200 μg/mL, pela quimiotaxia in vitro. Sugere-se potencial ação antimicrobiana frente a Staphylococcus aureus, nas concentrações de 250 e 500 mg/mL, com forma- ção de halo de inibição de 11 e 21 mm, respectivamente. Observou-se que o extrato de P. paniculata nas concentrações de 1, 10 e 50 μg/mL potencializou o crescimento de trofozoítos de Trichomonas vaginalis. Quanto aos ensaios sobre o SNC, verificou-se diminuição da ansiedade e aumento da atividade locomotora em animais tratados com doses de 125 e 250 mg/kg.
2010-01-01T00:00:00ZO presente trabalho teve como objetivo investigar o efeito anti-inflamatório, antimicrobiano, antiprotozoário e possível ação sobre o sistema nervoso central (SNC) em ratos tratados com extrato hidroalcoólico de Pfaffia paniculata. Verificou-se atividade anti-inflamatória tanto in vivo, na dose de 100 mg/kg, como in vitro nas concentrações de 50 e 100 μg/mL. Porém, verificou-se efeito pró-inflamatório na dose de 200 mg/kg, pelo ensaio de pleurisia e de 200 μg/mL, pela quimiotaxia in vitro. Sugere-se potencial ação antimicrobiana frente a Staphylococcus aureus, nas concentrações de 250 e 500 mg/mL, com forma- ção de halo de inibição de 11 e 21 mm, respectivamente. Observou-se que o extrato de P. paniculata nas concentrações de 1, 10 e 50 μg/mL potencializou o crescimento de trofozoítos de Trichomonas vaginalis. Quanto aos ensaios sobre o SNC, verificou-se diminuição da ansiedade e aumento da atividade locomotora em animais tratados com doses de 125 e 250 mg/kg.Toxicidade pré-clínica de fitoterápico com <i>Anemopaegma mirandum</i>, <i>Cola nitida</i>, <i>Passiflora alata</i>, <i>Paullinia cupana</i>, <i>Ptychopetalum olacoides</i> e tiaminaMello, Joao Roberto Braga deMello, Fernanda B. deLangeloh, Augustohttp://sedici.unlp.edu.ar:80/handle/10915/78712019-06-27T04:03:27Z2010-01-01T00:00:00ZArticulo
Pre-clinic toxicity of a phytoterapic containing anemopaegma mirandum, Cola nitida, Passiflora alata, Paullinia cupana, Ptychopetalum olacoides and Thiamin Chlorhydrate
Latin American Journal of Pharmacy; vol. 29, no. 1
A formulação fitoterápico contendo Anemopaegma mirandum (catuaba), Cola nitida (nóz de cola), Passiflora alata (maracujá), Paullinia cupana (guaraná), Ptychopetalum olacoides (marapuama) e cloridrato de tiamina (Nerviton®) foi investigada quanto aos potenciais efeitos tóxicos em doses repetidas quando administrada por via oral (gavagem) a ratos e ratas Wistar (machos por 30 dias e fêmeas por 44 dias, correspondendo à gestação e lactação). Nos estudos as dosagens diárias usadas foram 10 vezes as preconizadas para fins terapêuticos em seres humanos. Foram avaliados os sinais tóxicos de caráter geral, efeito sobre a deambulação, comportamento, sonolência, alterações de ritmo e freqüência respiratória, além das seguintes variáveis: avaliação da massa corporal, consumo de alimento e água, hemograma completo, análise bioquímica de sangue, exame anátomo-patológico com determinação da massa dos órgãos internos e, exame histológico quando da presença de alterações macroscópicas. Nas ratas, foram ainda avaliados: número e massa corporal de filhotes nascidos, presença de natimortos, mortes perinatal, malformações macroscópicas externas, desenvolvimento ponderal dos filhotes até o desmame, características de desenvolvimento geral, número de filhotes desmamados, massa dos órgãos internos das fêmeas e dos filhotes. Os resultados mostraram que a formulação fitoterápica contendo Anemopaegma mirandum (catuaba), Cola nitida (nóz de cola), Passiflora alata (maracujá), Paullinia cupana (guaraná), Ptychopetalum olacoides (marapuama) e cloridrato de tiamina (Nerviton®) não causou efeitos tóxicos quando administrado por via oral em doses repetidas durante 30 dias nos ratos e 44 dias nas ratas Wistar, incluindo gestação e lactação, em dose 10 vezes maior que a preconizada para fins terapêuticos em seres humanos. A formulação fitoterápica pode ser considerada relativamente inócua.; The phytotherapic formulation constituted by Anemopaegma mirandum (catuaba), Cola nitida (nóz de cola), Passiflora alata (maracujá) , Paullinia cupana (guaraná), Ptychopetalum olacoides (marapuama) and thiamin chlorhydrate (Nerviton®) was investigated from the potential of toxicological effects when orally administered to male (30 days) and female Wistar rats during the pregnancy and lactation (44 days). The daily oral dose was ten times the prescribed dosage to humans. The general signs of toxicity, locomotion, behavior, respiratory rate and rhythm were evaluated. Body weight, food and water intake, hematological and biochemical blood analysis, anatomopathological evaluation and visceral weight were measured. In female rats the litter size and weight, stillborn, perinatal deaths, external macroscopic malformations, ponderal development until to wean; general development and visceral weight were also investigated. The results interpreted as a whole revealed the absence of toxicological effects to the phytotherapic constituted by Anemopaegma mirandum (catuaba), Cola nitida (nóz de cola), Passiflora alata (maracujá), Paullinia cupana (guaraná), Ptychopetalum olacoides (marapuama) and thiamin chlorhydrate (Nerviton®) when administered to rats in a dose equivalent to 10 times the human dose. The phytotherapic can be considered relatively innocuous.
2010-01-01T00:00:00ZA formulação fitoterápico contendo Anemopaegma mirandum (catuaba), Cola nitida (nóz de cola), Passiflora alata (maracujá), Paullinia cupana (guaraná), Ptychopetalum olacoides (marapuama) e cloridrato de tiamina (Nerviton®) foi investigada quanto aos potenciais efeitos tóxicos em doses repetidas quando administrada por via oral (gavagem) a ratos e ratas Wistar (machos por 30 dias e fêmeas por 44 dias, correspondendo à gestação e lactação). Nos estudos as dosagens diárias usadas foram 10 vezes as preconizadas para fins terapêuticos em seres humanos. Foram avaliados os sinais tóxicos de caráter geral, efeito sobre a deambulação, comportamento, sonolência, alterações de ritmo e freqüência respiratória, além das seguintes variáveis: avaliação da massa corporal, consumo de alimento e água, hemograma completo, análise bioquímica de sangue, exame anátomo-patológico com determinação da massa dos órgãos internos e, exame histológico quando da presença de alterações macroscópicas. Nas ratas, foram ainda avaliados: número e massa corporal de filhotes nascidos, presença de natimortos, mortes perinatal, malformações macroscópicas externas, desenvolvimento ponderal dos filhotes até o desmame, características de desenvolvimento geral, número de filhotes desmamados, massa dos órgãos internos das fêmeas e dos filhotes. Os resultados mostraram que a formulação fitoterápica contendo Anemopaegma mirandum (catuaba), Cola nitida (nóz de cola), Passiflora alata (maracujá), Paullinia cupana (guaraná), Ptychopetalum olacoides (marapuama) e cloridrato de tiamina (Nerviton®) não causou efeitos tóxicos quando administrado por via oral em doses repetidas durante 30 dias nos ratos e 44 dias nas ratas Wistar, incluindo gestação e lactação, em dose 10 vezes maior que a preconizada para fins terapêuticos em seres humanos. A formulação fitoterápica pode ser considerada relativamente inócua.
The phytotherapic formulation constituted by Anemopaegma mirandum (catuaba), Cola nitida (nóz de cola), Passiflora alata (maracujá) , Paullinia cupana (guaraná), Ptychopetalum olacoides (marapuama) and thiamin chlorhydrate (Nerviton®) was investigated from the potential of toxicological effects when orally administered to male (30 days) and female Wistar rats during the pregnancy and lactation (44 days). The daily oral dose was ten times the prescribed dosage to humans. The general signs of toxicity, locomotion, behavior, respiratory rate and rhythm were evaluated. Body weight, food and water intake, hematological and biochemical blood analysis, anatomopathological evaluation and visceral weight were measured. In female rats the litter size and weight, stillborn, perinatal deaths, external macroscopic malformations, ponderal development until to wean; general development and visceral weight were also investigated. The results interpreted as a whole revealed the absence of toxicological effects to the phytotherapic constituted by Anemopaegma mirandum (catuaba), Cola nitida (nóz de cola), Passiflora alata (maracujá), Paullinia cupana (guaraná), Ptychopetalum olacoides (marapuama) and thiamin chlorhydrate (Nerviton®) when administered to rats in a dose equivalent to 10 times the human dose. The phytotherapic can be considered relatively innocuous.Potential effect of Citrus decumana extract on stress induced peptic ulcer in ratSood, ShailjaMuthuraman, ArunachalamArora, BhawnaBansal, StutiBali, ManojSharma, Pritam D.http://sedici.unlp.edu.ar:80/handle/10915/78702019-06-27T04:03:26Z2010-01-01T00:00:00ZArticulo
Latin American Journal of Pharmacy; vol. 29, no. 1
The present study was designed to investigate the antiulcer activity of ethyl acetate extract of Citrus decumana (grapefruit) peels. The antiulcerogenic activity was evaluated in water immersion and hypothermic restraint stress models at different doses (150, 250 and 350 mg/kg). The antiulcer potential of the extract was assessed by determining and comparing the ulcerative index and biochemical estimation was carried out using various oxidative stress markers i.e., TBARS, GSH, SOD and CAT in the blood and tissue samples. The highest dose (350 mg/kg) of the extract showed significant decrease in the ulcerative index and TBARS level, whereas there was increase in the GSH, SOD and CAT levels. Whereas the lowest and medium dose (150 mg/kg and 250mg/kg) did not produce any significant results. Therefore, our study indicate that the Citrus decumana peel extract may be used as a natural therapeutic agent in the treatment of peptic ulcers.
2010-01-01T00:00:00ZThe present study was designed to investigate the antiulcer activity of ethyl acetate extract of Citrus decumana (grapefruit) peels. The antiulcerogenic activity was evaluated in water immersion and hypothermic restraint stress models at different doses (150, 250 and 350 mg/kg). The antiulcer potential of the extract was assessed by determining and comparing the ulcerative index and biochemical estimation was carried out using various oxidative stress markers i.e., TBARS, GSH, SOD and CAT in the blood and tissue samples. The highest dose (350 mg/kg) of the extract showed significant decrease in the ulcerative index and TBARS level, whereas there was increase in the GSH, SOD and CAT levels. Whereas the lowest and medium dose (150 mg/kg and 250mg/kg) did not produce any significant results. Therefore, our study indicate that the Citrus decumana peel extract may be used as a natural therapeutic agent in the treatment of peptic ulcers.Caracteres anatômicos de folha e caule de Piper mikanianum (Kunth) Steud., PiperaceaeDuarte, Márcia do RocioSiebenrock, Maria C.N.http://sedici.unlp.edu.ar:80/handle/10915/78692019-06-27T04:03:25Z2010-01-01T00:00:00ZArticulo
Anatomical characters of the leaf and stem of Piper mikanianum (Kunth) Steud., Piperaceae
Latin American Journal of Pharmacy; vol. 29, no. 1
O gênero Piper L. (Piperaceae) ocorre em regiões tropicais e subtropicais, e inclui várias espécies de importância na medicina popular, podendo-se destacar Piper mikanianum (Kunth) Steud. Denominada de pariparoba e jaborandi, essa planta é empregada como antisséptico, hepatoprotetor e emenagogo. Este trabalho investigou a anatomia foliar e caulinar dessa potencial droga vegetal, a fim de estabelecer
caracteres estruturais para a identificação microscópica e para a diferenciação de outras espécies do gênero. Folhas adultas e caules jovens foram fixados, seccionados à mão livre ou emblocados em glicol-metacrilato e seccionados em micrótomo, e posteriormente corados. A folha possui epiderme uniestratificada, estômatos tetracíticos exclusivamente na face abaxial, tricomas glandulares e tectores, camadas subepidérmicas parenquimáticas, mesofilo dorsiventral, células secretoras e ráfides de oxalato de cálcio. A nervura central e o pecíolo apresentam feixes vasculares colaterais. No nível caulinar analisado, a epiderme persiste e o felogênio tem instalação periférica. Observam-se feixes vasculares colaterais dispostos em dois círculos,
o externo no cilindro vascular e o interno na medula. Na zona perimedular, ocorre uma bainha esclerenquimática
sinuosa e um canal está presente na região central.; The genus Piper L. (Piperaceae) is found in tropical and subtropical regions, and it includes several species of interest
in folk medicine, such as Piper mikanianum (Kunth) Steud. Commonly known as "pariparoba" or "jaborandi" in Portuguese, this plant is considered antiseptic, hepatoprotective and emmenagogue. This work has investigated the leaf and stem anatomy of this potential vegetal drug, in order to establish structural characters for
microscopic identification and for differentiating from other species of the genus. Mature leaves and young stems
were fixed, either sectioned by free-hand or embedded in glycol methacrylate and sectioned by microtome, and
then stained. The leaf has uniseriate epidermis, tetracytic stomata exclusively on the abaxial side, glandular and
non-glandular trichomes, parenchymatic sub-epidermal layers, dorsiventral mesophyll, secretory cells and calcium
oxalate raphides. The midrib and petiole show collateral vascular bundles. In the caulinar level analysed, the
epidermis remains and the phellogen has peripheral installation. There are collateral vascular bundles arranged in
two rings, the external one in the vascular cylinder and the internal one in the pith. In the perimedular zone, it occurs
a sinuous sclerenchymatic sheath and a canal is present in the central region.
2010-01-01T00:00:00ZO gênero Piper L. (Piperaceae) ocorre em regiões tropicais e subtropicais, e inclui várias espécies de importância na medicina popular, podendo-se destacar Piper mikanianum (Kunth) Steud. Denominada de pariparoba e jaborandi, essa planta é empregada como antisséptico, hepatoprotetor e emenagogo. Este trabalho investigou a anatomia foliar e caulinar dessa potencial droga vegetal, a fim de estabelecer
caracteres estruturais para a identificação microscópica e para a diferenciação de outras espécies do gênero. Folhas adultas e caules jovens foram fixados, seccionados à mão livre ou emblocados em glicol-metacrilato e seccionados em micrótomo, e posteriormente corados. A folha possui epiderme uniestratificada, estômatos tetracíticos exclusivamente na face abaxial, tricomas glandulares e tectores, camadas subepidérmicas parenquimáticas, mesofilo dorsiventral, células secretoras e ráfides de oxalato de cálcio. A nervura central e o pecíolo apresentam feixes vasculares colaterais. No nível caulinar analisado, a epiderme persiste e o felogênio tem instalação periférica. Observam-se feixes vasculares colaterais dispostos em dois círculos,
o externo no cilindro vascular e o interno na medula. Na zona perimedular, ocorre uma bainha esclerenquimática
sinuosa e um canal está presente na região central.
The genus Piper L. (Piperaceae) is found in tropical and subtropical regions, and it includes several species of interest
in folk medicine, such as Piper mikanianum (Kunth) Steud. Commonly known as "pariparoba" or "jaborandi" in Portuguese, this plant is considered antiseptic, hepatoprotective and emmenagogue. This work has investigated the leaf and stem anatomy of this potential vegetal drug, in order to establish structural characters for
microscopic identification and for differentiating from other species of the genus. Mature leaves and young stems
were fixed, either sectioned by free-hand or embedded in glycol methacrylate and sectioned by microtome, and
then stained. The leaf has uniseriate epidermis, tetracytic stomata exclusively on the abaxial side, glandular and
non-glandular trichomes, parenchymatic sub-epidermal layers, dorsiventral mesophyll, secretory cells and calcium
oxalate raphides. The midrib and petiole show collateral vascular bundles. In the caulinar level analysed, the
epidermis remains and the phellogen has peripheral installation. There are collateral vascular bundles arranged in
two rings, the external one in the vascular cylinder and the internal one in the pith. In the perimedular zone, it occurs
a sinuous sclerenchymatic sheath and a canal is present in the central region.