http://sedici.unlp.edu.ar:80/handle/10915/233 2026-08-17T17:30:38Z 2026-08-17T17:30:38Z Piantavini, Mário S. Stremel, Dile P. Trindade, Ângela C.L.B. Pontarolo, Roberto http://sedici.unlp.edu.ar:80/handle/10915/8092 2019-06-30T20:03:04Z 2010-01-01T00:00:00Z

Comunicacion Latin American Journal of Pharmacy; vol. 29, no. 8 A spectrophotometric method in the visible region was developed and validated for the quantification of kojic acid (KA) in raw materials and products. According to the validation results, this method yields linearity in the range of 5 and 50 μg/mL of KA (r = 0.99998), selectivity, suitable sensitivity, precision (RSD 1.33 % and 1.21 % for intermediate precision and repeatability respectively), accuracy (recovery near 100 %) and robustness (varying pH, temperature and reading time) were measured and led to the validation of this method.These methods were tested and validated for various parameters according to ICH guidelines and USP.

2010-01-01T00:00:00Z A spectrophotometric method in the visible region was developed and validated for the quantification of kojic acid (KA) in raw materials and products. According to the validation results, this method yields linearity in the range of 5 and 50 μg/mL of KA (r = 0.99998), selectivity, suitable sensitivity, precision (RSD 1.33 % and 1.21 % for intermediate precision and repeatability respectively), accuracy (recovery near 100 %) and robustness (varying pH, temperature and reading time) were measured and led to the validation of this method.These methods were tested and validated for various parameters according to ICH guidelines and USP. Joseph, Jince M. Sowndhararajan, Kandhasamy Loganayaki, Nataraj Manian, Sellamuthu http://sedici.unlp.edu.ar:80/handle/10915/8091 2019-06-30T20:03:04Z 2010-01-01T00:00:00Z

Comunicacion Latin American Journal of Pharmacy; vol. 29, no. 8 Total phenolics, tannins, flavonoids and the antioxidative properties of the traditionally used medicinal plant Naregamia alata Wight. & Arn. were assessed. The hot water extract contained higher levels of total phenolics, tannins and flavonoids. The extracts were subjected to assess their potential antioxidant activities using various in vitro systems such as DPPH^•, ABTS^•+, FRAP, β-carotene linoleic acid bleaching system, phosphomolybdenum reduction and Fe²⁺ chelation. It is concluded that N. alata may serve as a potential source of natural antioxidants capable of offering protection against free-radical mediated damages.

2010-01-01T00:00:00Z Total phenolics, tannins, flavonoids and the antioxidative properties of the traditionally used medicinal plant Naregamia alata Wight. & Arn. were assessed. The hot water extract contained higher levels of total phenolics, tannins and flavonoids. The extracts were subjected to assess their potential antioxidant activities using various in vitro systems such as DPPH^•, ABTS^•+, FRAP, β-carotene linoleic acid bleaching system, phosphomolybdenum reduction and Fe²⁺ chelation. It is concluded that N. alata may serve as a potential source of natural antioxidants capable of offering protection against free-radical mediated damages. Carrara, Vanessa da S. Souza, Amanda de Dias Filho, Benedito Prado Nakamura, Celso Vataru Paulo, Luís F. de Young, María C. M. Svidzinski, Terezinha I. E. García Cortez, Diógenes Aparício http://sedici.unlp.edu.ar:80/handle/10915/8090 2019-06-30T20:03:02Z 2010-01-01T00:00:00Z

Comunicacion Latin American Journal of Pharmacy; vol. 29, no. 8 The essential oil obtained from the leaves of Piper amalago L. by hydrodistillation was analyzed by CG-MS. The yield essential oil from fresh plant material was 0.1%. The main compounds found were β-copaen-4-α-ol (26 %), 7-epi-α-eudesmol (21.84 %), epi-α-cadinol (12.70 %), and n-hexyl-benzoate (12.29 %). The essential oil demonstrated antifungal activity against nine Candida strains, as shown using the agar-diffusion method.

2010-01-01T00:00:00Z The essential oil obtained from the leaves of Piper amalago L. by hydrodistillation was analyzed by CG-MS. The yield essential oil from fresh plant material was 0.1%. The main compounds found were β-copaen-4-α-ol (26 %), 7-epi-α-eudesmol (21.84 %), epi-α-cadinol (12.70 %), and n-hexyl-benzoate (12.29 %). The essential oil demonstrated antifungal activity against nine Candida strains, as shown using the agar-diffusion method. Lakshmana Prabu, S. Selvamani, P. Latha, S. http://sedici.unlp.edu.ar:80/handle/10915/8089 2019-06-30T20:03:01Z 2010-01-01T00:00:00Z

Comunicacion Latin American Journal of Pharmacy; vol. 29, no. 8 A new simple, rapid and reproducible high performance thin layer chromatographic method has been developed and validated for the analysis of granisetron hydrochloride (GSH) in bulk drug and from pharmaceutical formulation. The chromatographic separation was achieved on HPTLC aluminium plates precoated with silica gel 60F₂₅₄ as the stationary phase with chloroform: methanol (80:20 %v/v) as mobile phase. The method gives a compact band for GSH (Rf value of 0.45 ± 0.02). Densitometric analysis of GSH was carried out in the absorbance mode at 301 nm. The linear regression analysis data for the calibration plots showed good linear relationship with the correlation coefficient of 0.9980 with respect to peak area in the concentration range of 400-1600 ngband^-1. The method was validated for accuracy, precision, recovery studies, specificity, sensitivity, limit of detection and limit of quantification. Statistical analysis proved the method was precise, reproducible, selective, specific, and accurate for analysis of GSH. The wide linearity range, sensitivity, accuracy, and simple mobile phase composition imply the method is suitable for routine quantification of GSH with high precision and accuracy in bulk drug and marketed oral solid dosage form.

2010-01-01T00:00:00Z A new simple, rapid and reproducible high performance thin layer chromatographic method has been developed and validated for the analysis of granisetron hydrochloride (GSH) in bulk drug and from pharmaceutical formulation. The chromatographic separation was achieved on HPTLC aluminium plates precoated with silica gel 60F₂₅₄ as the stationary phase with chloroform: methanol (80:20 %v/v) as mobile phase. The method gives a compact band for GSH (Rf value of 0.45 ± 0.02). Densitometric analysis of GSH was carried out in the absorbance mode at 301 nm. The linear regression analysis data for the calibration plots showed good linear relationship with the correlation coefficient of 0.9980 with respect to peak area in the concentration range of 400-1600 ngband^-1. The method was validated for accuracy, precision, recovery studies, specificity, sensitivity, limit of detection and limit of quantification. Statistical analysis proved the method was precise, reproducible, selective, specific, and accurate for analysis of GSH. The wide linearity range, sensitivity, accuracy, and simple mobile phase composition imply the method is suitable for routine quantification of GSH with high precision and accuracy in bulk drug and marketed oral solid dosage form. Ravelo, Yazmin Molina, Vivian Carbajal, Daisy Arruzazabala, María de Lourdes Más, Rosa Oyarzábal, Ambar Pérez, Yohani Jiménez, Sonia http://sedici.unlp.edu.ar:80/handle/10915/8088 2019-06-30T20:02:56Z 2010-01-01T00:00:00Z

Comunicacion Latin American Journal of Pharmacy; vol. 29, no. 8 D-002 (beeswax alcohols) contains triacontanol as most abundant component. Local application of triacontanol has been shown anti-inflammatory effects on chemically-induced dermatitis and oral treatment with D-002 produced anti-inflammatory effects in carrageenan-induced pleurisy and cotton granuloma in rats, but its effects on xylene-induced mouse ear edema had not been studied. This study investigated the effects of single oral and topical doses of D-002 on this model. Oral dosing groups were composed by one negative control and six xylene-treated, one positive control, four D-002-treated (25, 50, 200, 400 mg/kg), and one indomethacin-treated (10 mg/kg). Topical dosing groups were conformed by one negative control and four xylene- treated, one positive control and three D-002-treated (2.5, 5, 10 %). Single oral doses of D-002 significantly and dose-dependently reduced edema formation and myeloperoxidase activity, but single topical applications unchanged both variables. Concluding, acute oral treatment with D- 002 was effective to decrease xylene-induced mouse ear edema, but single topical doses were ineffective.

2010-01-01T00:00:00Z D-002 (beeswax alcohols) contains triacontanol as most abundant component. Local application of triacontanol has been shown anti-inflammatory effects on chemically-induced dermatitis and oral treatment with D-002 produced anti-inflammatory effects in carrageenan-induced pleurisy and cotton granuloma in rats, but its effects on xylene-induced mouse ear edema had not been studied. This study investigated the effects of single oral and topical doses of D-002 on this model. Oral dosing groups were composed by one negative control and six xylene-treated, one positive control, four D-002-treated (25, 50, 200, 400 mg/kg), and one indomethacin-treated (10 mg/kg). Topical dosing groups were conformed by one negative control and four xylene- treated, one positive control and three D-002-treated (2.5, 5, 10 %). Single oral doses of D-002 significantly and dose-dependently reduced edema formation and myeloperoxidase activity, but single topical applications unchanged both variables. Concluding, acute oral treatment with D- 002 was effective to decrease xylene-induced mouse ear edema, but single topical doses were ineffective. Ramírez Maisuls, Hugo Enrique Monzón, Celina M. Delfino, Mario R. Sarno, María del C. http://sedici.unlp.edu.ar:80/handle/10915/8087 2019-06-30T20:02:54Z 2010-01-01T00:00:00Z

Comunicacion Latin American Journal of Pharmacy; vol. 29, no. 8 A spectrophotometric method is proposed for the analysis of atenolol, based on its reaction with the indicator phenol red in an acetone environment. Experimental conditions for the formation of an ion pair complex peaking at 388 nm were optimized. The method is linear in the range of 1 to 5 mg/mL with a correlation coefficient (n = 30) of 0.9968. Stoichiometry of the reaction is 1: 1. Free energy change (ΔG) for complex formation and stability constant (K_F) have been calculated. Working with placebo it was found that excipients do not interfere in the analysis. The proposed method was applied to tablets provided by the Medicinal Plant of Corrientes (Plamecor), Argentina. Recoveries from 99.67 to 100.17 were achieved. Results were compared (F test and t test) favorably with those given by official methods. (HPLC - UV).

2010-01-01T00:00:00Z A spectrophotometric method is proposed for the analysis of atenolol, based on its reaction with the indicator phenol red in an acetone environment. Experimental conditions for the formation of an ion pair complex peaking at 388 nm were optimized. The method is linear in the range of 1 to 5 mg/mL with a correlation coefficient (n = 30) of 0.9968. Stoichiometry of the reaction is 1: 1. Free energy change (ΔG) for complex formation and stability constant (K_F) have been calculated. Working with placebo it was found that excipients do not interfere in the analysis. The proposed method was applied to tablets provided by the Medicinal Plant of Corrientes (Plamecor), Argentina. Recoveries from 99.67 to 100.17 were achieved. Results were compared (F test and t test) favorably with those given by official methods. (HPLC - UV). Joshi, Vishal D. Bhat, Ishwar K. Jayaraman, R. Dahake, Akash P. Desai, Arvind R. http://sedici.unlp.edu.ar:80/handle/10915/8086 2019-06-30T20:02:54Z 2010-01-01T00:00:00Z

Comunicacion Latin American Journal of Pharmacy; vol. 29, no. 8 A series of some novel thiazolidinones derivatives were synthesized and evaluated for their pharmacological activities. Thiazolidinones were synthesized from p-nitro aniline in four steps. First Schiff’s bases (V1- 8) were prepared by reacting the N4 -(4-nitrophenyl) thiazole-2,4-diamine (3) of p-nitro aniline derivatives with different aromatic aldehydes. Cyclocondensation of the Schiff’s bases with thioglycolic acid in presence of anhydrous zinc chloride resulted in the formation of the corresponding thiazolidinone (VD1-8) analogues. The structures of the newly synthesized compounds have been established on the basis of their spectral data. The synthesized selected compounds were evaluated for their anti-inflammatory and analgesic activity.

2010-01-01T00:00:00Z A series of some novel thiazolidinones derivatives were synthesized and evaluated for their pharmacological activities. Thiazolidinones were synthesized from p-nitro aniline in four steps. First Schiff’s bases (V1- 8) were prepared by reacting the N4 -(4-nitrophenyl) thiazole-2,4-diamine (3) of p-nitro aniline derivatives with different aromatic aldehydes. Cyclocondensation of the Schiff’s bases with thioglycolic acid in presence of anhydrous zinc chloride resulted in the formation of the corresponding thiazolidinone (VD1-8) analogues. The structures of the newly synthesized compounds have been established on the basis of their spectral data. The synthesized selected compounds were evaluated for their anti-inflammatory and analgesic activity. Geroushi, Afaf Auzi, Abdurazag A. Elhwuegi, Abdalla S. Elzawam, Fawzi El-Sherif, Akram Nahar, Lutfun Sarker, Satyajit D. http://sedici.unlp.edu.ar:80/handle/10915/8085 2019-06-30T20:02:53Z 2010-01-01T00:00:00Z

Comunicacion Latin American Journal of Pharmacy; vol. 29, no. 8 Ferula hermonis (Apiaceae) is a well known Middle-Eastern medicinal plant. It has long been used traditionally as an aphrodisiac agent. The antinociceptive and anti-inflammatory activities of the root oil of F. hermonis were evaluated by the hot-plate test, the acetic acid-induced writhing test and the carragennan-induced rat paw edema test. In the hot-plate test, the root oil in oral doses of 400 and 800 mg/kg significantly increased the reaction time of animals to thermal pain, and in the acetic acid-induced writhing test, in similar oral doses it showed a considerable inhibition of acetic acid-induced writhing in mice in a dose-dependant manner. In the carrageenan induced paw oedema model, the oral administration of 50 and 100 mg/kg of F. hermonis root oil to adult Wister rats showed a statistically significant decrease in rat paw o induced by carrageenan 1 and 2 h after carrageenan injection.

2010-01-01T00:00:00Z Ferula hermonis (Apiaceae) is a well known Middle-Eastern medicinal plant. It has long been used traditionally as an aphrodisiac agent. The antinociceptive and anti-inflammatory activities of the root oil of F. hermonis were evaluated by the hot-plate test, the acetic acid-induced writhing test and the carragennan-induced rat paw edema test. In the hot-plate test, the root oil in oral doses of 400 and 800 mg/kg significantly increased the reaction time of animals to thermal pain, and in the acetic acid-induced writhing test, in similar oral doses it showed a considerable inhibition of acetic acid-induced writhing in mice in a dose-dependant manner. In the carrageenan induced paw oedema model, the oral administration of 50 and 100 mg/kg of F. hermonis root oil to adult Wister rats showed a statistically significant decrease in rat paw o induced by carrageenan 1 and 2 h after carrageenan injection. Mello, Joao Roberto Braga de Mello, Fernanda B. de Langeloh, Augusto http://sedici.unlp.edu.ar:80/handle/10915/8084 2019-06-30T20:02:49Z 2010-01-01T00:00:00Z

Comunicacion Latin American Journal of Pharmacy; vol. 29, no. 8 The phytotherapic formulation constituted by Anemopaegma mirandum (catuaba), Cola nitida (nóz de cola), Passiflora alata (maracujá), Paullinia cupana (guaraná), Ptychopetalum olacoides (marapuama) and thiamin chlorhydrate (Nerviton®) was investigated from the potential of toxicological effects when orally administered for 30 days to male and female New Zealand rabbits. The daily oral dose was ten times the prescribed dosage to humans. The general signs of toxicity, locomotion, behavior, respiratory rate and rhythm were evaluated. Body weight, food and water intake, rectal temperature, hematological and biochemical blood analysis, urinalysis, anatomopathological evaluation and visceral weight were measured. The results interpreted as a whole revealed the absence of toxicological effects to the phytotherapic studied when administered to New Zealand rabbits in a dose equivalent to 10 times the human dose and then can be considered relatively innocuous.

2010-01-01T00:00:00Z The phytotherapic formulation constituted by Anemopaegma mirandum (catuaba), Cola nitida (nóz de cola), Passiflora alata (maracujá), Paullinia cupana (guaraná), Ptychopetalum olacoides (marapuama) and thiamin chlorhydrate (Nerviton®) was investigated from the potential of toxicological effects when orally administered for 30 days to male and female New Zealand rabbits. The daily oral dose was ten times the prescribed dosage to humans. The general signs of toxicity, locomotion, behavior, respiratory rate and rhythm were evaluated. Body weight, food and water intake, rectal temperature, hematological and biochemical blood analysis, urinalysis, anatomopathological evaluation and visceral weight were measured. The results interpreted as a whole revealed the absence of toxicological effects to the phytotherapic studied when administered to New Zealand rabbits in a dose equivalent to 10 times the human dose and then can be considered relatively innocuous. Marín, Gustavo Horacio Cañás, Martín Carlson, Soledad Silvestrini, María Pía Corva, Santiago Gerardo Mestorino, Olga Nora Errecalde, Jorge Oscar http://sedici.unlp.edu.ar:80/handle/10915/8083 2020-07-29T00:44:59Z 2010-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 29, no. 8 The World Health Organization raises self-care as a strategy for health promotion. Although self-medication is included in self-care strategy, often it is associate to an irrational medicine's usage. This factor associated with illicit drugs and alcohol consumption becomes in a public health problem. In order to establish frequency of self-medication and the prevalence of illicit drugs and alcohol consumption among university students of La Plata, Argentina, was started the present project. Information was collected by anonymous survey, that evaluated age, sex, origin, cohabitants, type, frequency of consumption, and career. A total of 5170 students were polled. 46.64 % consumes regularly some medicine and 50.11 % of this consumption was self-medication. Mainly this consumption consisted in analgesics (88 %) and antibiotics (45 %). Benzodiazepines were also consumed in 6.9 % (39 % by self-medication). The consumption of illicit drugs was 38.1 % (29 % marihuana, 4 % cocaine, 1,2 % "paco" and 2.9 % "ecstasy"). The consumption of alcoholic drinks was 82 % for beer, 56 % fernet, 55 % wine and 48 % whiskey. Medicines were obtained outside pharmacies in 30.1 % of the cases. The major consumption of cocaine was given among students of Social Careers [Odds Ratio (OR) 2.3], and in those that lives without their families (1.5). The results indicate that self-medication is common among university students. Those students that live alone had a significant higher risk of being self-medicated than those that lives with their families. More than 30 % of the medicines were obtained at the informal market (other place that pharmacies).

2010-01-01T00:00:00Z The World Health Organization raises self-care as a strategy for health promotion. Although self-medication is included in self-care strategy, often it is associate to an irrational medicine's usage. This factor associated with illicit drugs and alcohol consumption becomes in a public health problem. In order to establish frequency of self-medication and the prevalence of illicit drugs and alcohol consumption among university students of La Plata, Argentina, was started the present project. Information was collected by anonymous survey, that evaluated age, sex, origin, cohabitants, type, frequency of consumption, and career. A total of 5170 students were polled. 46.64 % consumes regularly some medicine and 50.11 % of this consumption was self-medication. Mainly this consumption consisted in analgesics (88 %) and antibiotics (45 %). Benzodiazepines were also consumed in 6.9 % (39 % by self-medication). The consumption of illicit drugs was 38.1 % (29 % marihuana, 4 % cocaine, 1,2 % "paco" and 2.9 % "ecstasy"). The consumption of alcoholic drinks was 82 % for beer, 56 % fernet, 55 % wine and 48 % whiskey. Medicines were obtained outside pharmacies in 30.1 % of the cases. The major consumption of cocaine was given among students of Social Careers [Odds Ratio (OR) 2.3], and in those that lives without their families (1.5). The results indicate that self-medication is common among university students. Those students that live alone had a significant higher risk of being self-medicated than those that lives with their families. More than 30 % of the medicines were obtained at the informal market (other place that pharmacies). Cavallini, Daniela C. U. Abdalla, Dulcinéia S. P. Pauly-Silveira, Nadiége D. Rossi, Elizeu A. http://sedici.unlp.edu.ar:80/handle/10915/8082 2019-06-30T20:02:44Z 2010-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 29, no. 8 Oxidized Low-Density Lipoproteins (oxLDL) and autoantibodies against oxLDL are important in the development of atherosclerotic lesions. Statins are efficacious in the control of dyslipidemia and prevention of atherosclerosis; however, many questions concerning the mechanism of action of such drugs remain unknown. This work investigated the effect of simvastatin on generation of autoantibodies against oxLDL and development of atherosclerosis in rabbits. The animals were divided into three groups: control, hypercholesterolemic, and hypercholesterolemic simvastatin (3.0 mg simvastatin/ kg body weight). Concentrations of autoantibodies against oxLDL were determined on days 0, 30 and 60 of the experiment and the atherosclerotic lesions were evaluated at the end of the study. Simvastatin reduced intimal proliferation in the thoracic region, prevented arterial calcification and inhibited the generation of autoantibodies against oxLDL. In conclusion, daily administration of simvastatin slows down atherosclerotic lesion development in rabbits with induced hypercholesterolemia and inhibition on generation of autoantibodies against oxLDL contributes to the cardioprotective effect observed.

2010-01-01T00:00:00Z Oxidized Low-Density Lipoproteins (oxLDL) and autoantibodies against oxLDL are important in the development of atherosclerotic lesions. Statins are efficacious in the control of dyslipidemia and prevention of atherosclerosis; however, many questions concerning the mechanism of action of such drugs remain unknown. This work investigated the effect of simvastatin on generation of autoantibodies against oxLDL and development of atherosclerosis in rabbits. The animals were divided into three groups: control, hypercholesterolemic, and hypercholesterolemic simvastatin (3.0 mg simvastatin/ kg body weight). Concentrations of autoantibodies against oxLDL were determined on days 0, 30 and 60 of the experiment and the atherosclerotic lesions were evaluated at the end of the study. Simvastatin reduced intimal proliferation in the thoracic region, prevented arterial calcification and inhibited the generation of autoantibodies against oxLDL. In conclusion, daily administration of simvastatin slows down atherosclerotic lesion development in rabbits with induced hypercholesterolemia and inhibition on generation of autoantibodies against oxLDL contributes to the cardioprotective effect observed. Dias, Remeth J. Mali, Kailas K. Ghorpade, Vishwajeet S. Garje, Sandeep B. Havaldar, Vijay D. http://sedici.unlp.edu.ar:80/handle/10915/8081 2019-06-30T20:02:43Z 2010-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 29, no. 8 Gastroretentive Delivery Systems are employed to improve the bioavailability of drugs which are absorbed through upper part of GIT, by increasing their retention time. Incorporation of permeability enhancers in the formulations of such drugs can further increase their bioavailability; however their use in the formulations is questionable due to the toxicity exhibited by them. Acyclovir is a class III drug having low oral bioavailability due to improper absorption. Mucoadhesive tablets of acyclovir containing natural permeation enhancers were prepared by direct compression and evaluated for mucoadhesion strength, in-vitro dissolution parameters and in-vitro absorption studies. The formulations containing Aloe vera extract showed increase in the mucoadhesion strength and retarded the drug release. The in-vitro absorption studies revealed that the formulations containing Aloe vera extract (Enhancement Ratio 1.94) and chausath prahar pippal (Enhancement Ratio 1.87) showed significant increase in the permeation of the drug. The studies led to the conclusion that by formulating mucoadhesive tablets of acyclovir containing natural permeation enhancers increased the permeability, thus proving to be the cheaper and easily available alternative to the other permeation enhancers.

2010-01-01T00:00:00Z Gastroretentive Delivery Systems are employed to improve the bioavailability of drugs which are absorbed through upper part of GIT, by increasing their retention time. Incorporation of permeability enhancers in the formulations of such drugs can further increase their bioavailability; however their use in the formulations is questionable due to the toxicity exhibited by them. Acyclovir is a class III drug having low oral bioavailability due to improper absorption. Mucoadhesive tablets of acyclovir containing natural permeation enhancers were prepared by direct compression and evaluated for mucoadhesion strength, in-vitro dissolution parameters and in-vitro absorption studies. The formulations containing Aloe vera extract showed increase in the mucoadhesion strength and retarded the drug release. The in-vitro absorption studies revealed that the formulations containing Aloe vera extract (Enhancement Ratio 1.94) and chausath prahar pippal (Enhancement Ratio 1.87) showed significant increase in the permeation of the drug. The studies led to the conclusion that by formulating mucoadhesive tablets of acyclovir containing natural permeation enhancers increased the permeability, thus proving to be the cheaper and easily available alternative to the other permeation enhancers. Asane, Govind S. Yamsani, Madhusudan R. Bhatt, Jaykrishna H. Kolhe, Mahesh H. Mukkanti, Khagga http://sedici.unlp.edu.ar:80/handle/10915/8080 2019-06-30T20:02:43Z 2010-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 29, no. 8 The objective of present study was to improve gastric residence time of furazolidone by preparing mucoadhesive Multiple Unit Systems (MUS) with chitosan, Hydroxypropyl methyl cellulose K4M and sodium carboxymethyl cellulose by employing ionotropic gelation method. The resultant MUS were evaluated in vitro and in vivo. The particle size length ranged between 0.76 ± 0.25 to 0.89 ± 0.23mm, while the breadth was 0.76 ± 0.15 to 0.89 ± 0.06 mm, respectively. Encapsulation efficiency was in range of 82 to 90 %. MUS exhibited good mucoadhesive property in in vitro wash-off test. Stability studies showed no significant change in dissolution profiles (P < 0.05). The Gastrointestinal transit time was determined by fluoroscopic study which revealed that, the MUS retained in gastrointestinal tract for more than 5 hours and distributed throughout GIT. Based upon these results, prepared mucoadhesive MUS can be a good alternative to single unit systems to deliver Furazolidone with improved gastric residence time to treat intestinal amoebiasis.

2010-01-01T00:00:00Z The objective of present study was to improve gastric residence time of furazolidone by preparing mucoadhesive Multiple Unit Systems (MUS) with chitosan, Hydroxypropyl methyl cellulose K4M and sodium carboxymethyl cellulose by employing ionotropic gelation method. The resultant MUS were evaluated in vitro and in vivo. The particle size length ranged between 0.76 ± 0.25 to 0.89 ± 0.23mm, while the breadth was 0.76 ± 0.15 to 0.89 ± 0.06 mm, respectively. Encapsulation efficiency was in range of 82 to 90 %. MUS exhibited good mucoadhesive property in in vitro wash-off test. Stability studies showed no significant change in dissolution profiles (P < 0.05). The Gastrointestinal transit time was determined by fluoroscopic study which revealed that, the MUS retained in gastrointestinal tract for more than 5 hours and distributed throughout GIT. Based upon these results, prepared mucoadhesive MUS can be a good alternative to single unit systems to deliver Furazolidone with improved gastric residence time to treat intestinal amoebiasis. Lu, Wenzong Ni, Yuan Zhao, Chen Zhang, Liang Ren, Yumiao http://sedici.unlp.edu.ar:80/handle/10915/8079 2019-06-30T20:02:43Z 2010-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 29, no. 8 The object of the present study was to investigate the anticancer properties of total alkaloids from Solanum lyratum Thunb (SLT-A), including the inhibitory effect of SLT-A on HeLa cells and the apoptosis-inducing capacity in vitro. In our study, cytotoxicity was measured by the growth inhibition assay and detection of apoptosis was performed by Hoechst33324 and Tdt-mediated dUTP nick end labeling (TUNEL) staining assays. The in vitro cytotoxic studies were complemented by the cell cycle analysis and determination caspase-3 activity. Reverse transcription-polymerase chain reaction (RT-PCR) assay was applied on the expression of apoptosis-associated genes. The result showed that treatment of HeLa cells with SLT-A resulted in the growth inhibition effect, and the IC₅₀ value was approximately 82 µg/ml. SLTA (80 µg/ml) induced more cell apoptosis of HeLa cells and accumulated the cells in the G2/M phase compared with the control cells. On the other hand, the expression of p53 and Bax gene was increased in the cells treated with SLT-A (80 µg/ml), with an increase in the activity of caspase-3, while Bcl-2 expression was not changed compared to the control cells. Our results demonstrated that SLT-A presented antiproliferative activity in HeLa cells and might be a potential anticancer drug.

2010-01-01T00:00:00Z The object of the present study was to investigate the anticancer properties of total alkaloids from Solanum lyratum Thunb (SLT-A), including the inhibitory effect of SLT-A on HeLa cells and the apoptosis-inducing capacity in vitro. In our study, cytotoxicity was measured by the growth inhibition assay and detection of apoptosis was performed by Hoechst33324 and Tdt-mediated dUTP nick end labeling (TUNEL) staining assays. The in vitro cytotoxic studies were complemented by the cell cycle analysis and determination caspase-3 activity. Reverse transcription-polymerase chain reaction (RT-PCR) assay was applied on the expression of apoptosis-associated genes. The result showed that treatment of HeLa cells with SLT-A resulted in the growth inhibition effect, and the IC₅₀ value was approximately 82 µg/ml. SLTA (80 µg/ml) induced more cell apoptosis of HeLa cells and accumulated the cells in the G2/M phase compared with the control cells. On the other hand, the expression of p53 and Bax gene was increased in the cells treated with SLT-A (80 µg/ml), with an increase in the activity of caspase-3, while Bcl-2 expression was not changed compared to the control cells. Our results demonstrated that SLT-A presented antiproliferative activity in HeLa cells and might be a potential anticancer drug. Huang, Li-jing Gao, Wenyuan Li, Xia Man, Shuli Zhang, Yanjun Huang, Luqi Liu, Changxiao http://sedici.unlp.edu.ar:80/handle/10915/8078 2019-06-30T20:02:39Z 2010-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 29, no. 8 Bulbs of Fritillaria ussuriensis Maxim., usually known as Bulbus Fritillariae ussuriensis, (BFU) has been used as antitussive, antiasthmatic and expectorant in traditional herbal medicine. In this study, the aqueous extract of BFU (BFUE) was evaluated for its anti-inflammatory activity. Meanwhile, the content of PGE₂ and MDA in inflammatory exudates was measured to explore the anti-inflammatory mechanisms of BFUE. In order to identify the active components of BFU, the total alkaloids (TA), the total flavonoids (TF) and the total saponins (TS) were evaluated for their bioactivities. Results showed that BFUE inhibited carrageenin-induced paw edema, xylene-induced auricular edema and acetic acid-induced vascular permeation in a dose-dependent manner, and it revealed obvious inhibitory effects on the increase of PGE₂ and MDA. TF showed the highest anti-inflammatory effects on auricular edema induced by xylene in mice, and TS at a dose of 400 and 200 mg/kg also showed good effects (P < 0.01), but TA had no effect on this anti-inflammatory model. These results indicated that the Bulbus Fritillariae ussuriensis had good anti-inflammatory effects, which might be related to the reduction of PGE₂ and MDA levels, and TF and TS might be the active components for this activity.

2010-01-01T00:00:00Z Bulbs of Fritillaria ussuriensis Maxim., usually known as Bulbus Fritillariae ussuriensis, (BFU) has been used as antitussive, antiasthmatic and expectorant in traditional herbal medicine. In this study, the aqueous extract of BFU (BFUE) was evaluated for its anti-inflammatory activity. Meanwhile, the content of PGE₂ and MDA in inflammatory exudates was measured to explore the anti-inflammatory mechanisms of BFUE. In order to identify the active components of BFU, the total alkaloids (TA), the total flavonoids (TF) and the total saponins (TS) were evaluated for their bioactivities. Results showed that BFUE inhibited carrageenin-induced paw edema, xylene-induced auricular edema and acetic acid-induced vascular permeation in a dose-dependent manner, and it revealed obvious inhibitory effects on the increase of PGE₂ and MDA. TF showed the highest anti-inflammatory effects on auricular edema induced by xylene in mice, and TS at a dose of 400 and 200 mg/kg also showed good effects (P < 0.01), but TA had no effect on this anti-inflammatory model. These results indicated that the Bulbus Fritillariae ussuriensis had good anti-inflammatory effects, which might be related to the reduction of PGE₂ and MDA levels, and TF and TS might be the active components for this activity. Khan, Islam U. Ashfaq, Muhammad http://sedici.unlp.edu.ar:80/handle/10915/8077 2019-06-30T20:02:38Z 2010-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 29, no. 8 A simple, sensitive and reproducible RP-HPLC method was developed and validated for the simultaneous determination of atorvastatin and gemfibrozil in human plasma. After a single step liquidliquid extraction of both the drugs with acetonitrile, the separation was accomplished on a Merck C 18 column (250 x 4.6, 5 µm). Diode array detection at a wavelength of 240 nm was carried out with a mobile phase comprising of a mixture of 0.1M ammonium acetate buffer (pH 5.0) and acetonitrile in the ratio of (45:55, v/v). The method was linear in the concentration range of 0.1-20 µg/ml for atorvastatin and 6-1200 µg/ml for gemfibrozil with correlation coefficient between 0.9997 and 0.9976. The limit of detection was 0.03 µg/ml for atorvastatin and 1.8 µg/ml for gemfibrozil. The limit of quantitation was 0.1 µg/ml for atorvastatin and 6 µg/ml for gemfibrozil. The average recovery of both the analytes was greater than 75 %. The method was validated in terms of linearity, recovery, precision, specificity, LOD/LOQ values and stability of solutions and it was applied successfully for the determination of atorvastatin and gemfibrozil in spiked human plasma.

2010-01-01T00:00:00Z A simple, sensitive and reproducible RP-HPLC method was developed and validated for the simultaneous determination of atorvastatin and gemfibrozil in human plasma. After a single step liquidliquid extraction of both the drugs with acetonitrile, the separation was accomplished on a Merck C 18 column (250 x 4.6, 5 µm). Diode array detection at a wavelength of 240 nm was carried out with a mobile phase comprising of a mixture of 0.1M ammonium acetate buffer (pH 5.0) and acetonitrile in the ratio of (45:55, v/v). The method was linear in the concentration range of 0.1-20 µg/ml for atorvastatin and 6-1200 µg/ml for gemfibrozil with correlation coefficient between 0.9997 and 0.9976. The limit of detection was 0.03 µg/ml for atorvastatin and 1.8 µg/ml for gemfibrozil. The limit of quantitation was 0.1 µg/ml for atorvastatin and 6 µg/ml for gemfibrozil. The average recovery of both the analytes was greater than 75 %. The method was validated in terms of linearity, recovery, precision, specificity, LOD/LOQ values and stability of solutions and it was applied successfully for the determination of atorvastatin and gemfibrozil in spiked human plasma. Bermúdez, José M. Grau, Ricardo http://sedici.unlp.edu.ar:80/handle/10915/8076 2019-06-30T20:02:37Z 2010-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 29, no. 8 The purpose of this work is to explore the potential of combining poloxamer 407 and carrageenan for its utilization in an injectable depot drug release system. Reverse thermal gelation of these formulations allow the local injection in liquid form, gelling in situ after its administration. Carrageenan reinforces the structure of poloxamer gels (after 50 h of testing only 20 % of the system is eroding) and allows to modulate the release rate of progesterone as a function of formulations composition. The elastic modulus of sole poloxamer gels (G' = 56 Pa) increases significantly in presence of carrageenan (G' = 1 347 Pa) at 10 °C. The gelation temperature of tested formulations is between 17 and 22 °C and the gelation process is very quick. Poloxamer-carrageenan systems offer a promissory alternative approach to development of injectable depot systems for veterinary use.ies).

2010-01-01T00:00:00Z The purpose of this work is to explore the potential of combining poloxamer 407 and carrageenan for its utilization in an injectable depot drug release system. Reverse thermal gelation of these formulations allow the local injection in liquid form, gelling in situ after its administration. Carrageenan reinforces the structure of poloxamer gels (after 50 h of testing only 20 % of the system is eroding) and allows to modulate the release rate of progesterone as a function of formulations composition. The elastic modulus of sole poloxamer gels (G' = 56 Pa) increases significantly in presence of carrageenan (G' = 1 347 Pa) at 10 °C. The gelation temperature of tested formulations is between 17 and 22 °C and the gelation process is very quick. Poloxamer-carrageenan systems offer a promissory alternative approach to development of injectable depot systems for veterinary use.ies). Botelho, Marco A. Paixão, Mônica S. Rachid, Ítalo Bannet, Leonard Edward Patrus, Ana Helena Mattos, Thiago Borges Queiroz, Dinalva Ruela, Ronaldo Costa, Jose M. C Quintans Júnior, Lucindo José http://sedici.unlp.edu.ar:80/handle/10915/8075 2019-06-30T04:03:30Z 2010-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 29, no. 8 This study aimed to evaluate the effect of a diclofenac diethylammonium gel 10 mg/g (DD) on acute phase of ligature induced periodontitis model in rats. Experimental Periodontitis Disease (EPD) was induced in 30 Wistar rats subjected to ligature placement on left molars. Animals were treated with (DD), immediately after (EPD) induction. Saline-based gel (SG) was utilized as negative control and DD gel 10 mg/g was the tested substance. Animals were randomly assigned into the groups. The periodontium and the surrounding gingiva were examined at histopathology, as well as the neutrophil influx into the gingiva was assayed using myeloperoxidase activity levels by ELISA method. DD treatment reduced tissue lesion at histopathology coupled to decreased myeloperoxidase activity production in gingival tissue when compared to the saline gel control group (p < 0.05). The DD gel was able to provide a significant myeloperoxidase decreasing in gingiva tissue confirming to be effective in reducing gingival inflammation in this model.

2010-01-01T00:00:00Z This study aimed to evaluate the effect of a diclofenac diethylammonium gel 10 mg/g (DD) on acute phase of ligature induced periodontitis model in rats. Experimental Periodontitis Disease (EPD) was induced in 30 Wistar rats subjected to ligature placement on left molars. Animals were treated with (DD), immediately after (EPD) induction. Saline-based gel (SG) was utilized as negative control and DD gel 10 mg/g was the tested substance. Animals were randomly assigned into the groups. The periodontium and the surrounding gingiva were examined at histopathology, as well as the neutrophil influx into the gingiva was assayed using myeloperoxidase activity levels by ELISA method. DD treatment reduced tissue lesion at histopathology coupled to decreased myeloperoxidase activity production in gingival tissue when compared to the saline gel control group (p < 0.05). The DD gel was able to provide a significant myeloperoxidase decreasing in gingiva tissue confirming to be effective in reducing gingival inflammation in this model. Keservani, Raj K. Sharma, Anil K. Ramteke, Suman http://sedici.unlp.edu.ar:80/handle/10915/8074 2019-06-30T04:03:30Z 2010-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 29, no. 8 Niosomes have been reported as a possible approach to improve the low skin penetration and bioavailability characteristics shown by conventional topical vehicle for Baclofen (centrally acting skeletal muscle relaxant). Niosomes were prepared by lipid film hydration method using non ionic surfactant (Span 20) and cholesterol in varying molar ratios such as 1:1, 1:2 and 2:1. The prepared systems were characterized for vesicle surface morphology, entrapment efficiency, Osmotic fragility and stability studies. The in vitro drug release behavior was determined by an in-house fabricated dissolution-dialysis apparatus. The skeletal muscle relaxant activity was determined by rota rod method using Swiss albino mice. The average particle size of niosomes was in the range of 3260-3810 nm. The maximum percent drug entrapment was observed with span 20 (89.67 ± 0.46 %). Furthermore, the release profile of baclofen from these niosomes was 68.15 ± 1.7 % (maximum). The formulations kept for stability studies have exhibited percent drug entrapment value of 87.93 ± 0.34 % under refrigerated milieu (4 ± 2 °C). The mice treated with formulations have shown improved muscle relaxation activity which was evident by increased number of falls in rota rod test as compared to plane drug treated mice (p value = 0.001).

2010-01-01T00:00:00Z Niosomes have been reported as a possible approach to improve the low skin penetration and bioavailability characteristics shown by conventional topical vehicle for Baclofen (centrally acting skeletal muscle relaxant). Niosomes were prepared by lipid film hydration method using non ionic surfactant (Span 20) and cholesterol in varying molar ratios such as 1:1, 1:2 and 2:1. The prepared systems were characterized for vesicle surface morphology, entrapment efficiency, Osmotic fragility and stability studies. The in vitro drug release behavior was determined by an in-house fabricated dissolution-dialysis apparatus. The skeletal muscle relaxant activity was determined by rota rod method using Swiss albino mice. The average particle size of niosomes was in the range of 3260-3810 nm. The maximum percent drug entrapment was observed with span 20 (89.67 ± 0.46 %). Furthermore, the release profile of baclofen from these niosomes was 68.15 ± 1.7 % (maximum). The formulations kept for stability studies have exhibited percent drug entrapment value of 87.93 ± 0.34 % under refrigerated milieu (4 ± 2 °C). The mice treated with formulations have shown improved muscle relaxation activity which was evident by increased number of falls in rota rod test as compared to plane drug treated mice (p value = 0.001). Bunhak, Élcio Stoef, Paulo R. Melo, Eduardo B. de http://sedici.unlp.edu.ar:80/handle/10915/8073 2019-06-30T04:03:29Z 2010-01-01T00:00:00Z

Articulo Latin American Journal of Pharmacy; vol. 29, no. 8 The present paper aims to investigate the physicochemical quality of pharmaceutical drugs acquired and distributed by the Basic Pharmacy of the city of Cascavel-Paraná State. During the period between June 2004 and March 2006, samples of eight batches of acetylsalicylic acid 100 mg produced by two laboratories were analyzed. Following the techniques and specifications established by official compendiums, physicochemical tests of disintegration, dissolution, hardness, friability, uniformity of mass, content and limit of free acetylsalicylic acid were carried out. All the analyzed products presented quality deviations. All the six batches produced by one of the laboratories failed in the punctual dissolution test. The dissolution profile test confirmed such results, showing that these six batches cannot be considered equivalent to the branded drug.

2010-01-01T00:00:00Z The present paper aims to investigate the physicochemical quality of pharmaceutical drugs acquired and distributed by the Basic Pharmacy of the city of Cascavel-Paraná State. During the period between June 2004 and March 2006, samples of eight batches of acetylsalicylic acid 100 mg produced by two laboratories were analyzed. Following the techniques and specifications established by official compendiums, physicochemical tests of disintegration, dissolution, hardness, friability, uniformity of mass, content and limit of free acetylsalicylic acid were carried out. All the analyzed products presented quality deviations. All the six batches produced by one of the laboratories failed in the punctual dissolution test. The dissolution profile test confirmed such results, showing that these six batches cannot be considered equivalent to the branded drug.