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dc.date.accessioned 2021-09-14T18:28:30Z
dc.date.available 2021-09-14T18:28:30Z
dc.date.issued 2021-02
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/124820
dc.description.abstract The stretch of cardiac muscle increases developed force in two phases. The first phase occurs immediately after stretch and is the expression of the Frank-Starling mechanism, while the second one or slow force response (SFR) occurs gradually and is due to an increase in the calcium transient amplitude. An important step in the chain of events leading to the SFR generation is the increased production of reactive oxygen species (ROS) leading to redox sensitive ERK1/2, p90RSK, and NHE1 phosphorylation/activation. Conversely, suppression of ROS production blunts the SFR. The purpose of this study was to explore whether overexpression of the ubiquitously expressed antioxidant molecule thioredoxin-1 (TRX1) affects the SFR development and NHE1 phosphorylation. We did not detect any change in basal phopho-ERK1/2, phopho-p90RSK, and NHE1 expression in mice with TRX1 overexpression compared to wild type (WT). Isolated papillary muscles from WT or TRX1-overexpressing mice were stretched from 92 to 98% of its maximal length. A prominent SFR was observed in WT mice that was completely canceled in TRX1 animals. Interestingly, myocardial stretch induced a significant increase in NHE1 phosphorylation in WT mice that was not detected in TRX1-overexpressing mice. These novel results suggest that magnification of cardiac antioxidant defense power by overexpression of TRX1 precludes NHE1 phosphorylation/activation after stretch, consequently blunting the SFR development. en
dc.language en es
dc.subject TRX1 es
dc.subject SFR es
dc.subject NHE1 es
dc.subject antioxidant es
dc.subject cardiac hypertrophy es
dc.title Thioredoxin 1 (TRX1) Overexpression Cancels the Slow Force Response (SFR) Development en
dc.type Articulo es
sedici.identifier.other pmid:33718450 es
sedici.identifier.other doi:10.3389/fcvm.2021.622583 es
sedici.identifier.other pmcid:PMC7952880 es
sedici.identifier.issn 2297-055X es
sedici.creator.person Zavala, Maite Raquel es
sedici.creator.person Díaz, Romina Gisel es
sedici.creator.person Villa Abrille, María Celeste es
sedici.creator.person Pérez, Néstor Gustavo es
sedici.subject.materias Medicina es
sedici.description.fulltext true es
mods.originInfo.place Facultad de Ciencias Médicas es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution 4.0 International (CC BY 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Frontiers in Cardiovascular Medicine es
sedici.relation.journalVolumeAndIssue vol. 8 es

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Creative Commons Attribution 4.0 International (CC BY 4.0) Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International (CC BY 4.0)