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dc.date.accessioned 2019-06-24T18:56:02Z
dc.date.available 2019-06-24T18:56:02Z
dc.date.issued 2017
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/77030
dc.description.abstract The plant hormone salicylic acid (SA) is required for defense responses. NON EXPRESSER OF PATHOGENESIS RELATED 1 (NPR1) and NON RECOGNITION OF BTH-4 (NRB4) are required for the response to SA in Arabidopsis (Arabidopsis thaliana). Here, we isolated several interactors of NRB4 using yeast two-hybrid assays. Two of these interactors, βCA1 and βCA2, are β-carbonic anhydrase family proteins. Since double mutant βca1 βca2 plants did not show any obvious phenotype, we investigated other βCAs and found that NRB4 also interacts with βCA3 and βCA4. Moreover, several βCAs interacted with NPR1 in yeast, including one that interacted in a SA-dependent manner. This interaction was abolished in loss-of-function alleles of NPR1. Interactions between βCAs and both NRB4 and NPR1 were also detected in planta, with evidence for a triple interaction, NRB4- βCA1-NPR1. The quintuple mutant βca1 βca2 βca3 βca4 βca6 showed partial insensitivity to SA. These findings suggest that one of the functions of carbonic anhydrases is to modulate the perception of SA in plants. es
dc.format.extent 29 p. es
dc.language en es
dc.subject Arabidopsis thaliana, yeast, point mutation, protein interaction, phenotypes en
dc.subject Plantas es
dc.title β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis en
dc.type Articulo es
sedici.identifier.doi 10.1371/journal.pone.0181820
sedici.identifier.handle 11746/7528
sedici.creator.person Medina Puche, Laura es
sedici.creator.person Castelló, María José es
sedici.creator.person Canet, Juan Vicente es
sedici.creator.person Lamilla, Julián es
sedici.creator.person Colombo, María Laura es
sedici.creator.person Tornero, Pablo es
sedici.subject.materias Ciencias Exactas es
sedici.description.fulltext true es
mods.originInfo.place Facultad de Ciencias Exactas es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution 4.0 International (CC BY 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle PLoS ONE es
sedici.relation.journalVolumeAndIssue vol. 12, no. 7 es

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Creative Commons Attribution 4.0 International (CC BY 4.0) Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International (CC BY 4.0)