http://sedici.unlp.edu.ar:80/handle/10915/134 2026-10-11T19:00:17Z 2026-10-11T19:00:17Z Maffione, Leonardo Miguenz, Marcela Bellucci, Sebastián A. Betti, Pablo Torselli, María Laura Frutos, Elizabeth Staneloni, Maria Celia Vecchio, Gisella Greco, Analía Longarini, Valeria Bocchio, Sandra Filinger, Ester Julia http://sedici.unlp.edu.ar:80/handle/10915/6679 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Contribucion a revista Training in drug administration Acta Farmacéutica Bonaerense; vol. 23, no. 3 Con la finalidad de resolver problemas inherentes a la administración de medicamentos, se llevó a cabo un Curso de "Administración de Medicamentos" en el Hospital Universitario "Hospital de Clínicas José de San Martín" en Noviembre de 2001. Los talleres aportaron nuevos conceptos para el trabajo diario de los asistentes. El curso fue útil para la identificación, prevención y resolución de problemas relacionados con los medicamentos. Los temas se eligieron en base a las áreas de más consultas y donde se detectaban mayor número de errores tanto en la administración como en la prescripción. Se desarrollaron los siguientes temas: generalidades de las vías de administración y de los vasodilatadores, antibióticos, antiinflamatorios, analgésicos, antiulcerosos y farmacovigilancia.; With the aim to resolve problems inherent to drug administration, a Course of "Drug Administration" was carried out in the University Hospital "Hospital de Clínicas José de San Martín" in November 2001. The different workshops contributed to new concepts for the daily work of the assistants. The course was useful for the identification, prevention and resolution of problems related to drugs. The topics were chosen based on the areas with the most consultations and where the major number of errors were detected both in the administration and in the prescription. The following subjects were developed: basic knowledge of the routes of administration and of vasodilators, antibiotics, anti-inflammatories, analgesics, antiulcerous and pharmacovigilance.

2004-01-01T00:00:00Z Con la finalidad de resolver problemas inherentes a la administración de medicamentos, se llevó a cabo un Curso de "Administración de Medicamentos" en el Hospital Universitario "Hospital de Clínicas José de San Martín" en Noviembre de 2001. Los talleres aportaron nuevos conceptos para el trabajo diario de los asistentes. El curso fue útil para la identificación, prevención y resolución de problemas relacionados con los medicamentos. Los temas se eligieron en base a las áreas de más consultas y donde se detectaban mayor número de errores tanto en la administración como en la prescripción. Se desarrollaron los siguientes temas: generalidades de las vías de administración y de los vasodilatadores, antibióticos, antiinflamatorios, analgésicos, antiulcerosos y farmacovigilancia. With the aim to resolve problems inherent to drug administration, a Course of "Drug Administration" was carried out in the University Hospital "Hospital de Clínicas José de San Martín" in November 2001. The different workshops contributed to new concepts for the daily work of the assistants. The course was useful for the identification, prevention and resolution of problems related to drugs. The topics were chosen based on the areas with the most consultations and where the major number of errors were detected both in the administration and in the prescription. The following subjects were developed: basic knowledge of the routes of administration and of vasodilators, antibiotics, anti-inflammatories, analgesics, antiulcerous and pharmacovigilance. Lúcio Hirata, Lilian Sato, Mayumi Eliza Otsuka de Moraes Santos, Cid Aimbiré http://sedici.unlp.edu.ar:80/handle/10915/6678 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Contribucion a revista Cutaneous ageing and free radicals Acta Farmacéutica Bonaerense; vol. 23, no. 3 Durante o envelhecimento cronológico cutâneo, ocorre a modificação do material genético e a proliferação celular decresce resultando na perda de elasticidade, da capacidade de regular o metabolismo e a replicação do tecido se torna menos eficiente. Oxidações químicas e enzimáticas envolvendo a formação de radicais livres aceleram esse fenômeno, gerando estresse oxidativo, cujo maior dano é a peroxidação dos ácidos graxos da dupla camada lipídica levando à morte celular. Para evitar esse processo, a pele possui seu próprio mecanismo de defesa. Entretanto, a capacidade protetora desse mecanismo diminui com o envelhecimento e compostos exógenos podem reforçar a proteção natural. Este artigo tem por objetivo revisar esses mecanismos de senescência e de ampliar a visão geral do profissional farmacêutico ajudando-o a desenvolver pesquisas que visem a prevenção do envelhecimento precoce cutâneo.; During the chronological cutaneous aging, modifications on the genetic material and cellular proliferation decreases result on lossing of elasticity and in the ability to regulate metabolism. Also, tissue replications become less efficient. Oxidations from chemical and enzymatic reactions involving the formation of free radicals accelerate this phenomenon, generating an oxidative stress. Peroxidation of fatty acids of the double lipid layer is one of the main damage, resulting on cellular death. In order to prevent oxidative stress, the skin has its own defense mechanism. However, the protective ability of this mechanism decreases with aging, and exogenous compounds may enhance this natural protection. The aim of this article is to review these senescence mechanisms and to amplify the general view of pharmacists, helping them to develop researches for prevention of cutaneous aging.

2004-01-01T00:00:00Z Durante o envelhecimento cronológico cutâneo, ocorre a modificação do material genético e a proliferação celular decresce resultando na perda de elasticidade, da capacidade de regular o metabolismo e a replicação do tecido se torna menos eficiente. Oxidações químicas e enzimáticas envolvendo a formação de radicais livres aceleram esse fenômeno, gerando estresse oxidativo, cujo maior dano é a peroxidação dos ácidos graxos da dupla camada lipídica levando à morte celular. Para evitar esse processo, a pele possui seu próprio mecanismo de defesa. Entretanto, a capacidade protetora desse mecanismo diminui com o envelhecimento e compostos exógenos podem reforçar a proteção natural. Este artigo tem por objetivo revisar esses mecanismos de senescência e de ampliar a visão geral do profissional farmacêutico ajudando-o a desenvolver pesquisas que visem a prevenção do envelhecimento precoce cutâneo. During the chronological cutaneous aging, modifications on the genetic material and cellular proliferation decreases result on lossing of elasticity and in the ability to regulate metabolism. Also, tissue replications become less efficient. Oxidations from chemical and enzymatic reactions involving the formation of free radicals accelerate this phenomenon, generating an oxidative stress. Peroxidation of fatty acids of the double lipid layer is one of the main damage, resulting on cellular death. In order to prevent oxidative stress, the skin has its own defense mechanism. However, the protective ability of this mechanism decreases with aging, and exogenous compounds may enhance this natural protection. The aim of this article is to review these senescence mechanisms and to amplify the general view of pharmacists, helping them to develop researches for prevention of cutaneous aging. Bezerra Carvalho, Ana Cecilia Guedes Fernandes, Marianne Vasconcelos Santos, Eliane Jucielly Fernandes Monteiro de Mello, Arquimedes Medeiros, Isac Almeida de Formiga Melo Diniz, Margareth de Fátima http://sedici.unlp.edu.ar:80/handle/10915/6677 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Articulo Accuracy of information on herbal medicines (phytoterapeutics agents) advertisements published in Paraíba, Brazil Acta Farmacéutica Bonaerense; vol. 23, no. 3 Este estudo tem como objetivo avaliar a adequação legal da propaganda e publicidade de medicamentos fitoterápicos anunciada na Paraíba através de peças publicitárias obtidas na forma de impressos, os quais foram coletados em consultórios médicos, hospitais públicos e privados, farmácias e congressos. Verificou-se que as 53 propagandas coletadas promoveram 351 fitoterápicos, anunciando 140 plantas diferentes. A análise do material evidenciou irregularidades que ferem a legislação vigente: a RDC N° 102/2000 e a RDC N° 48/2004 da ANVISA (Agência Nacional de Vigilância Sanitária), regulamentadoras da propaganda de medicamentos e do registro de fitoterápicos respectivamente. A promoção de propagandas mais éticas para medicamentos é de interesse da ANVISA, além de ser extremamente importante para a população que é sempre a maior vítima das irregularidades.; The aim of this study is to evaluate the advertisements accuracy of herbal medicines (phytoterapeutic agents) published in Paraíba through data obtained in the form of printed ads (leaflets) collected at medical clinics, public and private hospitals, drugstores and medical congress. It was verified that the 53 collected ads promoted 351 herbal medicines, announcing 140 different plants. The analysis of the ads evidenced irregularities according to Brazilian regulatory guidelines: RDC N° 102/2000 and RDC N° 48/2004, wich regulate advertisements and herbal medicines respectively. The promotion of more ethical ads for medicines is one the most important interest of the ANVISA (National Agency of Sanitary Surveillance), besides being an extremely important fact for the population that is always the largest victim of the irregularities.

2004-01-01T00:00:00Z Este estudo tem como objetivo avaliar a adequação legal da propaganda e publicidade de medicamentos fitoterápicos anunciada na Paraíba através de peças publicitárias obtidas na forma de impressos, os quais foram coletados em consultórios médicos, hospitais públicos e privados, farmácias e congressos. Verificou-se que as 53 propagandas coletadas promoveram 351 fitoterápicos, anunciando 140 plantas diferentes. A análise do material evidenciou irregularidades que ferem a legislação vigente: a RDC N° 102/2000 e a RDC N° 48/2004 da ANVISA (Agência Nacional de Vigilância Sanitária), regulamentadoras da propaganda de medicamentos e do registro de fitoterápicos respectivamente. A promoção de propagandas mais éticas para medicamentos é de interesse da ANVISA, além de ser extremamente importante para a população que é sempre a maior vítima das irregularidades. The aim of this study is to evaluate the advertisements accuracy of herbal medicines (phytoterapeutic agents) published in Paraíba through data obtained in the form of printed ads (leaflets) collected at medical clinics, public and private hospitals, drugstores and medical congress. It was verified that the 53 collected ads promoted 351 herbal medicines, announcing 140 different plants. The analysis of the ads evidenced irregularities according to Brazilian regulatory guidelines: RDC N° 102/2000 and RDC N° 48/2004, wich regulate advertisements and herbal medicines respectively. The promotion of more ethical ads for medicines is one the most important interest of the ANVISA (National Agency of Sanitary Surveillance), besides being an extremely important fact for the population that is always the largest victim of the irregularities. Milián Vázquez, Pedro Miguel Cepero Pérez, Ivette Espinosa Brito, Alfredo Padrón Velásquez, Luis Hernández Fernández, Juana Linares Borges, Arlette Reyes Hernández, Dunia Vázquez Montero, Lidia Quirós Enríquez, Maira Mass Sosa, Luis Alberto http://sedici.unlp.edu.ar:80/handle/10915/6676 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Articulo Characterisation of the digoxin consumption in the cardiac insufficiency in Rodas Municipality (Cienfuegos, Cuba) Acta Farmacéutica Bonaerense; vol. 23, no. 3 Se presenta un estudio descriptivo de corte transversal en pacientes mayores de 15 años con insuficiencia cardíaca que fueron tratados con digoxina en 15 consultorios médicos del municipio de Rodas, Cienfuegos, Cuba, en el primer semestre del 2003. Se estudiaron 130 pacientes registrados en la Farmacia Municipal. Se realizó una revisión de las historias clínicas individuales, hojas de egresos hospitalarios y entrevistas a los pacientes. La mayor parte de los pacientes que consumían digoxina eran hombres de 76 años o mayores que pertenecían a las clases II y III. Prevaleció la insuficiencia cardíaca congestiva. Todos utilizaban la digoxina a la dosis establecida y la mayoría llevaban más de 6 años consumiéndola. Se detectaron 7 intoxicaciones por digitálicos. El 63,6 % de los pacientes de la clase I tenían una prescripción incorrecta de la digoxina. Los grupos farmacológicos más usados junto al digitálico fueron los diuréticos, los nitritos y los inhibidores de la enzima convertidora de angiotensina (IECAs).; This is a descriptive transversal study of patients older than 15 years age with cardiac insufficiency that were treated in 15 consultation offices in the urban area of Rodas Municipality, Cienfuegos, Cuba, in the first semester of 2003. One hundred and thirty patients were studied, who were registered in the database of the municipality community pharmacy. It was carried out the revision of clinical records and patient interviews. The greatest amount of patients who took digoxin were men aged 76 and older classified within class II and III. The diagnosis of congestive heart failure was frequent. All the patients were prescribed with the established digoxin doses and the time of consumption was more than 6 years. Seven patients were intoxicated with digitalics. An incorrect prescription of digoxin corresponded to 63.6% of the patients class I. The pharmacological groups more used with digoxin were diuretics, nitrates and the angiotensin-converting enzymes.

2004-01-01T00:00:00Z Se presenta un estudio descriptivo de corte transversal en pacientes mayores de 15 años con insuficiencia cardíaca que fueron tratados con digoxina en 15 consultorios médicos del municipio de Rodas, Cienfuegos, Cuba, en el primer semestre del 2003. Se estudiaron 130 pacientes registrados en la Farmacia Municipal. Se realizó una revisión de las historias clínicas individuales, hojas de egresos hospitalarios y entrevistas a los pacientes. La mayor parte de los pacientes que consumían digoxina eran hombres de 76 años o mayores que pertenecían a las clases II y III. Prevaleció la insuficiencia cardíaca congestiva. Todos utilizaban la digoxina a la dosis establecida y la mayoría llevaban más de 6 años consumiéndola. Se detectaron 7 intoxicaciones por digitálicos. El 63,6 % de los pacientes de la clase I tenían una prescripción incorrecta de la digoxina. Los grupos farmacológicos más usados junto al digitálico fueron los diuréticos, los nitritos y los inhibidores de la enzima convertidora de angiotensina (IECAs). This is a descriptive transversal study of patients older than 15 years age with cardiac insufficiency that were treated in 15 consultation offices in the urban area of Rodas Municipality, Cienfuegos, Cuba, in the first semester of 2003. One hundred and thirty patients were studied, who were registered in the database of the municipality community pharmacy. It was carried out the revision of clinical records and patient interviews. The greatest amount of patients who took digoxin were men aged 76 and older classified within class II and III. The diagnosis of congestive heart failure was frequent. All the patients were prescribed with the established digoxin doses and the time of consumption was more than 6 years. Seven patients were intoxicated with digitalics. An incorrect prescription of digoxin corresponded to 63.6% of the patients class I. The pharmacological groups more used with digoxin were diuretics, nitrates and the angiotensin-converting enzymes. Bellucci, Sebastián A. Filinger, Ester Julia http://sedici.unlp.edu.ar:80/handle/10915/6675 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Articulo The role of the pharmacist in the use of radiologic contrast materials Acta Farmacéutica Bonaerense; vol. 23, no. 3 Las drogas utilizadas para medios de contraste se han empleado rutinariamente para urogramas excretores, Tomografía Axial Computada (TAC) y angiografía en los últimos treinta años. Si bien son drogas relativamente seguras, las reacciones adversas a medios de contraste (RAMc) ocurren y llevan muchas veces a importantes consecuencias en el paciente y en el sistema de salud. Para prevenir reacciones adversas es necesario fijar estrategias de selección de su uso basadas en el perfil del paciente. El papel del farmacéutico es desarrollar medidas de prevención y selección para el uso de contrastes radiológicos, así como la provisión y control de estos fármacos usados para diagnóstico.; Drugs employed as contrast media have been routinely used for excretory urography, CT and angiography in the last 30 years. Though they are relatively safe drugs, adverse reactions can be expected and many times led to important consequences in patients and in the health system. In order to prevent these adverse reactions, it is necessary to establish selection criteria for their use based on the patient's profile. The pharmacy's role is to the develop prevention and selection criteria in the use of radiological contrast media, as much as the provision and control of these drugs used for diagnosis.

2004-01-01T00:00:00Z Las drogas utilizadas para medios de contraste se han empleado rutinariamente para urogramas excretores, Tomografía Axial Computada (TAC) y angiografía en los últimos treinta años. Si bien son drogas relativamente seguras, las reacciones adversas a medios de contraste (RAMc) ocurren y llevan muchas veces a importantes consecuencias en el paciente y en el sistema de salud. Para prevenir reacciones adversas es necesario fijar estrategias de selección de su uso basadas en el perfil del paciente. El papel del farmacéutico es desarrollar medidas de prevención y selección para el uso de contrastes radiológicos, así como la provisión y control de estos fármacos usados para diagnóstico. Drugs employed as contrast media have been routinely used for excretory urography, CT and angiography in the last 30 years. Though they are relatively safe drugs, adverse reactions can be expected and many times led to important consequences in patients and in the health system. In order to prevent these adverse reactions, it is necessary to establish selection criteria for their use based on the patient's profile. The pharmacy's role is to the develop prevention and selection criteria in the use of radiological contrast media, as much as the provision and control of these drugs used for diagnosis. González Castillo, Tahimí Castellanos Gil, Eddy http://sedici.unlp.edu.ar:80/handle/10915/6674 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Articulo Development of a lisinopril (5 mg) formulation Acta Farmacéutica Bonaerense; vol. 23, no. 3 Se describe el desarrollo de diferentes variantes tecnológicas de tabletas de Lisinopril (5 mg de dosis). Se evaluó el efecto de diferentes excipientes sobre la calidad tecnológica de los comprimidos a partir de un diseño de experimento 22. Se describe un estudio comparativo de dos procedimientos de elaboración: compresión directa y granulación húmeda. El estudio demostró que es factible la elaboración de este producto por ambos métodos.; Development of different formulations of lisinopril (5 mg) is described. Factorial experimental design (22) was employed in order to evaluate the effect of different excipients on the quality of tablets. A comparative study for two manufactured process is described. Obtained results show that direct compression and wet granulation process can be used for development of Lisinopril 5 mg tablets.

2004-01-01T00:00:00Z Se describe el desarrollo de diferentes variantes tecnológicas de tabletas de Lisinopril (5 mg de dosis). Se evaluó el efecto de diferentes excipientes sobre la calidad tecnológica de los comprimidos a partir de un diseño de experimento 22. Se describe un estudio comparativo de dos procedimientos de elaboración: compresión directa y granulación húmeda. El estudio demostró que es factible la elaboración de este producto por ambos métodos. Development of different formulations of lisinopril (5 mg) is described. Factorial experimental design (22) was employed in order to evaluate the effect of different excipients on the quality of tablets. A comparative study for two manufactured process is described. Obtained results show that direct compression and wet granulation process can be used for development of Lisinopril 5 mg tablets. Volonté, María Guillermina Viñas, María Alicia Mordujovich de Buschiazzo, Perla Piersante, María Victoria Escales, María C. Gorriti, Carolina E. http://sedici.unlp.edu.ar:80/handle/10915/6673 2023-12-21T13:18:33Z 2004-01-01T00:00:00Z

Articulo Comparative study of carbamazepine 200 mg tablets, for pharmaceutical equivalence determination Acta Farmacéutica Bonaerense; vol. 23, no. 3 Carbamacepina (CBZ) es utilizada como antiepiléptico, analgésico y antimaníaco y por su estrecho margen terapéutico está clasificada como de Alto Riesgo Sanitario en nuestro país. El objetivo del presente trabajo fue realizar un estudio comparativo de las trece especialidades medicinales, CBZ 200 mg comprimidos, presentes en el mercado argentino y aprobadas para su comercialización, con el propósito de establecer equivalencia farmacéutica entre ellas. Se realizaron los siguientes ensayos: evaluación de rótulos y prospectos, descripción de los comprimidos, identidad del principio activo, contenido de CBZ, contenido de agua, uniformidad de unidades de dosificación, ensayo de disolución y perfiles de disolución. Se concluyó que entre todas las especialidades existe equivalencia farmacéutica con respecto a los ensayos de calidad realizados, aunque no todas presentan la misma velocidad de disolución. Con respecto a la información sobre las condiciones de almacenamiento, detallada en prospectos y envases secundarios, los productos identificados como A, B, I, J y M no son equivalentes al resto. Esta información es considerada de suma importancia ya que es crítica para la estabilidad de la CBZ y también para la intercambiabilidad de los productos.; Carbamazepine (CBZ) is used as antiepileptic, analgesic and antimaniac and the fact is that is a drug with narrow therapeutic window, and it is classified as a high risk drug in our country. The aim of this study was to compare the thirteen products, CBZ tablets 200 mg, which were available in the argentine market, looking to the pharmaceutical equivalence between them. The following tests were made: evaluation of packaging specifications, description of tablets, CBZ identification and quantification, water content, uniformity of dosage units, dissolution test and dissolution profiles. We conclude that there is pharmaceutical equivalence between all the studied products respect quality control assays, but some products shows differences in the rate of de dissolution. With regards to the information about the storage conditions, the products identified as A, B, I, J and M are not equivalents respect the others. This information is very important because it is critical to CBZ stability and for the potential interchangeability of the products.

2004-01-01T00:00:00Z Carbamacepina (CBZ) es utilizada como antiepiléptico, analgésico y antimaníaco y por su estrecho margen terapéutico está clasificada como de Alto Riesgo Sanitario en nuestro país. El objetivo del presente trabajo fue realizar un estudio comparativo de las trece especialidades medicinales, CBZ 200 mg comprimidos, presentes en el mercado argentino y aprobadas para su comercialización, con el propósito de establecer equivalencia farmacéutica entre ellas. Se realizaron los siguientes ensayos: evaluación de rótulos y prospectos, descripción de los comprimidos, identidad del principio activo, contenido de CBZ, contenido de agua, uniformidad de unidades de dosificación, ensayo de disolución y perfiles de disolución. Se concluyó que entre todas las especialidades existe equivalencia farmacéutica con respecto a los ensayos de calidad realizados, aunque no todas presentan la misma velocidad de disolución. Con respecto a la información sobre las condiciones de almacenamiento, detallada en prospectos y envases secundarios, los productos identificados como A, B, I, J y M no son equivalentes al resto. Esta información es considerada de suma importancia ya que es crítica para la estabilidad de la CBZ y también para la intercambiabilidad de los productos. Carbamazepine (CBZ) is used as antiepileptic, analgesic and antimaniac and the fact is that is a drug with narrow therapeutic window, and it is classified as a high risk drug in our country. The aim of this study was to compare the thirteen products, CBZ tablets 200 mg, which were available in the argentine market, looking to the pharmaceutical equivalence between them. The following tests were made: evaluation of packaging specifications, description of tablets, CBZ identification and quantification, water content, uniformity of dosage units, dissolution test and dissolution profiles. We conclude that there is pharmaceutical equivalence between all the studied products respect quality control assays, but some products shows differences in the rate of de dissolution. With regards to the information about the storage conditions, the products identified as A, B, I, J and M are not equivalents respect the others. This information is very important because it is critical to CBZ stability and for the potential interchangeability of the products. Staub, Inara Bergold, Ana Maria http://sedici.unlp.edu.ar:80/handle/10915/6672 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Articulo Determination of ketoconazole in shampoo by high performance liquid chromatography Acta Farmacéutica Bonaerense; vol. 23, no. 3 Fue desarrollado un procedimiento para cuantificar el ketoconazol en champú mediante cromatografia líquida de alta eficiencia. La fase móvil consiste en una mezcla de las siguientes soluciones: (A) monoisopropilamina-metanol (2:500 v/v) y (B) acetato de amônio-água (1:200 w/v) (7:3 v/v). El pH de la solución final fue ajustado a 5.5 con ácido acético. Fué utilizada una columna LiChrospher® 100 RP-8 (150 x 46 mm, 5 µm), flujo 1.0 mL/min, y detector UV (225 nm). La validación del método ha producido resultados adecuados. La desviación estándar relativa fue de 0,42% y la exactitud de 97,2%. La curva de calibración presentó linearidad entre las concentraciones de 60 y 480 µg/mL, con coeficiente de correlación de 0,9999.; A high performance liquid chromatography isocratic procedure was developed for the assay of ketoconazole in shampoo. Two solutions, (A) monoisopropylamine-methanol (2:500, v/v) and (B) ammonium acetate-water (1:200, w/v) were prepared. The mobile phase is a mixture of A and B (7:3, v/v), with pH adjusted to 5.5 with acetic acid. The chromatographic system consists of a LiChrospher® 100 RP-8 column (150 x 46 mm, 5 µm), flow rate of 1.0 mL/min and UV detector at 225 nm. The method validation yielded good results. Relative standard deviation (RSD) of 0.42% and accuracy of 97.2% were obtained. Calibration curve was linear between 60.0 and 480.0 µg/mL, with correlation coefficient of 0.9999.

2004-01-01T00:00:00Z Fue desarrollado un procedimiento para cuantificar el ketoconazol en champú mediante cromatografia líquida de alta eficiencia. La fase móvil consiste en una mezcla de las siguientes soluciones: (A) monoisopropilamina-metanol (2:500 v/v) y (B) acetato de amônio-água (1:200 w/v) (7:3 v/v). El pH de la solución final fue ajustado a 5.5 con ácido acético. Fué utilizada una columna LiChrospher® 100 RP-8 (150 x 46 mm, 5 µm), flujo 1.0 mL/min, y detector UV (225 nm). La validación del método ha producido resultados adecuados. La desviación estándar relativa fue de 0,42% y la exactitud de 97,2%. La curva de calibración presentó linearidad entre las concentraciones de 60 y 480 µg/mL, con coeficiente de correlación de 0,9999. A high performance liquid chromatography isocratic procedure was developed for the assay of ketoconazole in shampoo. Two solutions, (A) monoisopropylamine-methanol (2:500, v/v) and (B) ammonium acetate-water (1:200, w/v) were prepared. The mobile phase is a mixture of A and B (7:3, v/v), with pH adjusted to 5.5 with acetic acid. The chromatographic system consists of a LiChrospher® 100 RP-8 column (150 x 46 mm, 5 µm), flow rate of 1.0 mL/min and UV detector at 225 nm. The method validation yielded good results. Relative standard deviation (RSD) of 0.42% and accuracy of 97.2% were obtained. Calibration curve was linear between 60.0 and 480.0 µg/mL, with correlation coefficient of 0.9999. Antonelli-Ushirobira, Tânia Mara Yamaguti, Elza Uhemura, Leila Mariko Mello, João Carlos Palazzo de http://sedici.unlp.edu.ar:80/handle/10915/6671 2016-03-30T17:48:11Z 2004-01-01T00:00:00Z

Articulo Quality control of samples of Paullinia cupana H.B.K. var. sorbilis (Mart.) Ducke Acta Farmacéutica Bonaerense; vol. 23, no. 3 Sementes de guaraná [Paullinia cupana H.B.K. var. sorbilis (Mart.) Ducke], obtidas de lugares diferentes, foram analisadas quanto aos aspectos físico-químicos (perda por dessecação, teor de extrativos, teor de resíduo seco) e quanto ao teor de taninos totais e metilxantinas. A análise cromatográfica por CCD mostrou um valor de R_f = 0,43 para cafeína e 0,72 e 0,71 para catequina e epicatequina, respectivamente. A análise por CLAE apresentou, para uma fração semipurificada, tempos de retenção de catequina, epicatequina e cafeína de 6,17, 8,85 e 11,91 min, respectivamente. Assim, pode-se confirmar a qualidade e semelhança em teores químicos entre as diferentes matérias-primas vegetais.; Seeds of guaraná [Paullinia cupana H.B.K. var. sorbilis (Mart.) Ducke], obtained from different localities, were tested in regard to physicochemical aspects (loss on drying, determination of extracts, dry residue) and their total tannin and methylxanthine contents. TLC chromatographic analysis showed a Rf = 0.43 for caffeine and R_f = 0.72 and 0.71 for catechin and epicatechin respectively. Analysis of the semi-purified fraction by HPLC showed retention times for catechin, epicatechin and caffeine of 6.17, 8.85 and 11.91 min respectively. The quality and similarity of the chemical components of the raw material from different sources was confirmed.

2004-01-01T00:00:00Z Sementes de guaraná [Paullinia cupana H.B.K. var. sorbilis (Mart.) Ducke], obtidas de lugares diferentes, foram analisadas quanto aos aspectos físico-químicos (perda por dessecação, teor de extrativos, teor de resíduo seco) e quanto ao teor de taninos totais e metilxantinas. A análise cromatográfica por CCD mostrou um valor de R_f = 0,43 para cafeína e 0,72 e 0,71 para catequina e epicatequina, respectivamente. A análise por CLAE apresentou, para uma fração semipurificada, tempos de retenção de catequina, epicatequina e cafeína de 6,17, 8,85 e 11,91 min, respectivamente. Assim, pode-se confirmar a qualidade e semelhança em teores químicos entre as diferentes matérias-primas vegetais. Seeds of guaraná [Paullinia cupana H.B.K. var. sorbilis (Mart.) Ducke], obtained from different localities, were tested in regard to physicochemical aspects (loss on drying, determination of extracts, dry residue) and their total tannin and methylxanthine contents. TLC chromatographic analysis showed a Rf = 0.43 for caffeine and R_f = 0.72 and 0.71 for catechin and epicatechin respectively. Analysis of the semi-purified fraction by HPLC showed retention times for catechin, epicatechin and caffeine of 6.17, 8.85 and 11.91 min respectively. The quality and similarity of the chemical components of the raw material from different sources was confirmed. Rugna, Ana Zulema Gurni, Alberto Angel Wagner, Marcelo Luis http://sedici.unlp.edu.ar:80/handle/10915/6670 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Comunicacion Comparative studies on flavonoids from the aerial organs of Smilax Campestris Griseb. -Smilacaceae- Acta Farmacéutica Bonaerense; vol. 23, no. 3 En un estudio fitoquímico comparativo de los distintos órganos de una especie vegetal es posible establecer variaciones en la producción de metabolitos secundarios. El objetivo de este trabajo es verificar si existen diferencias de tipo cuali o cuantitativas en la producción de flavonoides en los diferentes órganos aéreos de Smilax campestris Griseb. -Smilacaceae-. Los resultados obtenidos demuestran que la hoja es el órgano que produce mayor concentración de compuestos, tanto de flavonoles como de proantocianidinas. Las variaciones evidenciadas fueron de tipo cuantitativo y no cualitativo. De estas apreciaciones surge que la constancia en la producción de estos compuestos hace que puedan ser útiles como parámetros para el control de calidad de S. campestris como planta o de extractos derivados de ella.; In a phytochemical comparative study of different organs of a plant differences in the secondary metabolites production may appear. The aim of this paper is to check if there are quali-quantitative differences in the different aerial organs in the flavonoid production of Smilax campestris Griseb. -Smilacaceae-. According to the results, the leaves produce the higher flavonols and proanthocyanidines concentration. There were no evidences of qualitative variations. The results were reproducible in all analyzed specimens. On the basis of these facts can be assumed that flavonoids can be used for quality control of S. campestris, either in the drug or in its extracts.

2004-01-01T00:00:00Z En un estudio fitoquímico comparativo de los distintos órganos de una especie vegetal es posible establecer variaciones en la producción de metabolitos secundarios. El objetivo de este trabajo es verificar si existen diferencias de tipo cuali o cuantitativas en la producción de flavonoides en los diferentes órganos aéreos de Smilax campestris Griseb. -Smilacaceae-. Los resultados obtenidos demuestran que la hoja es el órgano que produce mayor concentración de compuestos, tanto de flavonoles como de proantocianidinas. Las variaciones evidenciadas fueron de tipo cuantitativo y no cualitativo. De estas apreciaciones surge que la constancia en la producción de estos compuestos hace que puedan ser útiles como parámetros para el control de calidad de S. campestris como planta o de extractos derivados de ella. In a phytochemical comparative study of different organs of a plant differences in the secondary metabolites production may appear. The aim of this paper is to check if there are quali-quantitative differences in the different aerial organs in the flavonoid production of Smilax campestris Griseb. -Smilacaceae-. According to the results, the leaves produce the higher flavonols and proanthocyanidines concentration. There were no evidences of qualitative variations. The results were reproducible in all analyzed specimens. On the basis of these facts can be assumed that flavonoids can be used for quality control of S. campestris, either in the drug or in its extracts. Paula, Josiane P. Farago, Paulo V. Checchia, Lizianne E. M. Hirose, Karla M. Ribas, Joao L. http://sedici.unlp.edu.ar:80/handle/10915/6669 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Comunicacion Repellent activity of Ocimum selloi Benth. essential oil (variety eugenol) against anopheles braziliensis chagas Acta Farmacéutica Bonaerense; vol. 23, no. 3 Ocimum selloi Benth. (Lamiaceae) é uma planta herbácea nativa do Brasil. Seu óleo essencial tem sido utilizado como flavorizante em comidas e em produtos orais e como componente de fragrâncias. O objetivo do presente trabalho foi avaliar a atividade repelente de mosquitos do óleo essencial de O. selloi (variedade eugenol) em humanos. O óleo essencial foi obtido por hidrodestilação das folhas da espécie. Em estudo de campo, no qual seis voluntários (cada qual como seu próprio controle) foram expostos ao Anopheles brasiliensis Chagas por trinta minutos, O. selloi (10% v/v, solução em etanol) reduziu drasticamente o número de picadas comparado ao controle (redução de 98,1%, P=0,011), mostrando ser um repelente de mosquitos eficiente.; Ocimum selloi Benth. (Lamiaceae) is an herbal plant native to Brazil. The essential oil has been used as a flavoring additive in foods and oral products and as a component of fragrances. The aim of this study was to evaluate the mosquito repellent activity of O. selloi essential oil (variety eugenol) in humans. The essential oil was obtained by hydro-distillation of O. selloi leaves. In a field study in which six volunteers (each individual as his own control) were exposed to Anopheles brasiliensis Chagas for thirty minutes, O. selloi (10% v/v, solution in ethanol) drastically reduced the frequency of bites as compared to ethanol alone (reduction of 98.1%, P=0.011) thereby proving to be an effective mosquito repellent.

2004-01-01T00:00:00Z Ocimum selloi Benth. (Lamiaceae) é uma planta herbácea nativa do Brasil. Seu óleo essencial tem sido utilizado como flavorizante em comidas e em produtos orais e como componente de fragrâncias. O objetivo do presente trabalho foi avaliar a atividade repelente de mosquitos do óleo essencial de O. selloi (variedade eugenol) em humanos. O óleo essencial foi obtido por hidrodestilação das folhas da espécie. Em estudo de campo, no qual seis voluntários (cada qual como seu próprio controle) foram expostos ao Anopheles brasiliensis Chagas por trinta minutos, O. selloi (10% v/v, solução em etanol) reduziu drasticamente o número de picadas comparado ao controle (redução de 98,1%, P=0,011), mostrando ser um repelente de mosquitos eficiente. Ocimum selloi Benth. (Lamiaceae) is an herbal plant native to Brazil. The essential oil has been used as a flavoring additive in foods and oral products and as a component of fragrances. The aim of this study was to evaluate the mosquito repellent activity of O. selloi essential oil (variety eugenol) in humans. The essential oil was obtained by hydro-distillation of O. selloi leaves. In a field study in which six volunteers (each individual as his own control) were exposed to Anopheles brasiliensis Chagas for thirty minutes, O. selloi (10% v/v, solution in ethanol) drastically reduced the frequency of bites as compared to ethanol alone (reduction of 98.1%, P=0.011) thereby proving to be an effective mosquito repellent. Temprano, Guillermo D'Aquino, Miguel http://sedici.unlp.edu.ar:80/handle/10915/6668 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Comunicacion The Adenosin Triphosphate [ATP] reaction as a biological dirty indicator Acta Farmacéutica Bonaerense; vol. 23, no. 3 Por medio de la bioluminiscencia se puede detectar la cantidad de ATP presente en una superficie. Esto se relaciona directamente con la suciedad biológica, entendiéndose por tal a la constituída por sustancias orgánicas de origen vegetal o animal, incluyéndose a los microorganismos. Es opinión generalizada que el ATP es rápidamente destruído luego de la muerte celular por acción de la ATPasa. En la presente comunicación se publican resultados que ponen en discusión esta afirmación. Se concluye que en las condiciones de trabajo descriptas, la bioluminiscencia es muy sensible para determinar materia orgánica de origen animado, pero es poco sensible para los microorganismos en particular.; ATP present on a surface can be quantified by means of bioluminiscence. This is directly related with biological dirty, understanding by such the one composed by organic substances from vegetal or animal origin, including microorganisms. It is a general opinion that ATP is quickly destroyed after celular death, by action of ATPasa. In the present communication results are published that put this affirmation in discussion. It is concluded that under the mentioned work conditions, bioluminiscence is very sensitive for animated origin organic matter, but is of very low sensitivity for microorganisms in particular.

2004-01-01T00:00:00Z Por medio de la bioluminiscencia se puede detectar la cantidad de ATP presente en una superficie. Esto se relaciona directamente con la suciedad biológica, entendiéndose por tal a la constituída por sustancias orgánicas de origen vegetal o animal, incluyéndose a los microorganismos. Es opinión generalizada que el ATP es rápidamente destruído luego de la muerte celular por acción de la ATPasa. En la presente comunicación se publican resultados que ponen en discusión esta afirmación. Se concluye que en las condiciones de trabajo descriptas, la bioluminiscencia es muy sensible para determinar materia orgánica de origen animado, pero es poco sensible para los microorganismos en particular. ATP present on a surface can be quantified by means of bioluminiscence. This is directly related with biological dirty, understanding by such the one composed by organic substances from vegetal or animal origin, including microorganisms. It is a general opinion that ATP is quickly destroyed after celular death, by action of ATPasa. In the present communication results are published that put this affirmation in discussion. It is concluded that under the mentioned work conditions, bioluminiscence is very sensitive for animated origin organic matter, but is of very low sensitivity for microorganisms in particular. Bedascarrasbure, Enrique Maldonado, Luis Álvarez, Alejandro Rodríguez, Edgardo http://sedici.unlp.edu.ar:80/handle/10915/6667 2015-02-04T16:18:38Z 2004-01-01T00:00:00Z

Comunicacion Content of phenols and flavonoids in the argentinean propolis Acta Farmacéutica Bonaerense; vol. 23, no. 3 Los polifenoles y los flavonoides son considerados los principales compuestos bioactivos del propóleos, ya que se ha demostrado que la ingesta de los mismos reduce el riesgo de enfermedades cardiovasculares. Las abejas elaboran el propóleos a partir de resinas y otros materiales vegetales, por lo tanto la composición del mismo variará según la flora del lugar. Este trabajo fue desarrollado para determinar el contenido de estos compuestos en propóleos provenientes de diferentes regiones del país. Los resultados preliminares obtenidos permiten afirmar que el Propóleos Argentino presenta un elevado contenido de polifenoles y flavonoides, comparados con los datos reportados en la bibliografía.; Poliphenols and flavonoids are considered the main bioactive components of propolis, because these compounds reduce the risk of coronary heart disease. It is known that Apis mellifera collects resins from several plants, therefore the chemical composition of propolis will vary according to the flora of each region. This work was carried out in order to evaluate the content of these compounds in the Argentinean Propolis from different regions in the country. The preliminary results allow to affirm that the Argentinean Propolis presents a higher content of biologically active compounds than propolis from other regions in the world.

2004-01-01T00:00:00Z Los polifenoles y los flavonoides son considerados los principales compuestos bioactivos del propóleos, ya que se ha demostrado que la ingesta de los mismos reduce el riesgo de enfermedades cardiovasculares. Las abejas elaboran el propóleos a partir de resinas y otros materiales vegetales, por lo tanto la composición del mismo variará según la flora del lugar. Este trabajo fue desarrollado para determinar el contenido de estos compuestos en propóleos provenientes de diferentes regiones del país. Los resultados preliminares obtenidos permiten afirmar que el Propóleos Argentino presenta un elevado contenido de polifenoles y flavonoides, comparados con los datos reportados en la bibliografía. Poliphenols and flavonoids are considered the main bioactive components of propolis, because these compounds reduce the risk of coronary heart disease. It is known that Apis mellifera collects resins from several plants, therefore the chemical composition of propolis will vary according to the flora of each region. This work was carried out in order to evaluate the content of these compounds in the Argentinean Propolis from different regions in the country. The preliminary results allow to affirm that the Argentinean Propolis presents a higher content of biologically active compounds than propolis from other regions in the world. Oliveira, Simone Q. de Dal-Pizzol, Felipe Moreira, José C. F. Schenkel, Eloir Paulo Gosmann, Grace http://sedici.unlp.edu.ar:80/handle/10915/6666 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Comunicacion Antioxidant activity of Baccharis spicata, Baccharis trimera and Baccharis usterii Acta Farmacéutica Bonaerense; vol. 23, no. 3 Para determinar la actividad antioxidante de los extractos y de las fracciones de Baccharis spicata, B. trimera y B. usterii se emplearon los ensayos TRAP e TBARS. Los extractos acuosos de B. spicata e B. trimera, a una concentración de 25 µg/mL, presentaron actividad antioxidante mayor que Trolox® (1 mM) y para el extracto acuoso de B. usterii se observó una actividad semejante al Trolox®. Las fracciones fueron ensayadas a una concentración de 2,5 µg/mL. En TBARS todos los extractos fueron efectivos en la prevención de la peroxidación lipídica a través de la inhibición de la producción de especies reactivas del ácido tiobarbitúrico y de la mortalidad de las células por el peróxido de hidrógeno.; Antioxidant activities of extracts and fractions from Baccharis spicata, B. trimera and B. usterii were determined using TRAP and TBARS assays. Aqueous extracts from B. spicata and B. trimera, at a concentration of 25 µg/mL, showed a higher antioxidant activity when compared to Trolox® (1 mM) and aqueous extract from B. usterii exhibit similar activity than Trolox®. Fractions were tested using 2.5 µg/mL. In TBARS all extracts and fractions were effective in the prevention of lipid peroxidation by inhibiting the formation of thiobarbituric acid reactives species and cell mortality induced by hydrogen peroxide.

2004-01-01T00:00:00Z Para determinar la actividad antioxidante de los extractos y de las fracciones de Baccharis spicata, B. trimera y B. usterii se emplearon los ensayos TRAP e TBARS. Los extractos acuosos de B. spicata e B. trimera, a una concentración de 25 µg/mL, presentaron actividad antioxidante mayor que Trolox® (1 mM) y para el extracto acuoso de B. usterii se observó una actividad semejante al Trolox®. Las fracciones fueron ensayadas a una concentración de 2,5 µg/mL. En TBARS todos los extractos fueron efectivos en la prevención de la peroxidación lipídica a través de la inhibición de la producción de especies reactivas del ácido tiobarbitúrico y de la mortalidad de las células por el peróxido de hidrógeno. Antioxidant activities of extracts and fractions from Baccharis spicata, B. trimera and B. usterii were determined using TRAP and TBARS assays. Aqueous extracts from B. spicata and B. trimera, at a concentration of 25 µg/mL, showed a higher antioxidant activity when compared to Trolox® (1 mM) and aqueous extract from B. usterii exhibit similar activity than Trolox®. Fractions were tested using 2.5 µg/mL. In TBARS all extracts and fractions were effective in the prevention of lipid peroxidation by inhibiting the formation of thiobarbituric acid reactives species and cell mortality induced by hydrogen peroxide. Oltramari Toledo, Ana Cristina Duarte, Márcia do Rocio Nakashima, Tomoe http://sedici.unlp.edu.ar:80/handle/10915/6665 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Articulo Leaf morpho-anatomical characterization of Symphytum officinale L. (Boraginaceae) Acta Farmacéutica Bonaerense; vol. 23, no. 3 Symphytum officinale L. é uma planta herbácea, cujo nome popular mais conhecido no Brasil é confrei. Suas folhas são empregadas na fitoterapia como antiinflamatório, emoliente e anestésico suave de uso tópico. Com a finalidade de contribuir com a identificação da planta medicinal recomendada pela RDC N° 17/2000 - ANVISA, foram analisadas a morfologia externa e a anatomia da folha. O material foi fixado, seccionado à mão livre e corado ou submetido a testes histoquímicos. A folha é ovalado-oblonga ou lanceolada, com ápice agudo ou acuminado, base cuneada e margem levemente ondulada. A epiderme é uniestratificada, com estômatos anomocíticos, tricomas tectores unicelulares retos e glandulares capitados em ambas as faces. Tricomas tectores unicelulares unciformes ocorrem na superfície abaxial. Mesofilo dorsiventral, células com mucilagem, nervura central apresentando um a oito feixes vasculares colaterais, e pecíolo com vários feixes colaterais em arco aberto são observados.; Symphytum officinale L. is a herbaceous species, commonly known as comfrey in Brazil. Its leaves are employed in phytotherapy as topical anti-inflammatory, emollient and mild anesthetics. Aiming to contribute to the medicinal plant identification, which was recommended by RDC N° 17/2000 - ANVISA, the leaf external morphology and anatomy were carried out. The material was fixed, free-hand sectioned, either stained or undergone histochemical tests. The leaf is ovate-oblong or lanceolate, with acute or acuminate apex, cuneate base and slightly wavy margin. The epidermis is uniseriate, having anomocytic stomata, straight unicellular non-glandular trichomes and capitate glandular ones on both sides. Unciform unicellular non-glandular trichomes occur on the lower surface. Dorsiventral mesophyll, cells containing mucilage, midrib showing from one to eight collateral bundles, and petiole having various collateral bundles distributed as an open arc are observed.

2004-01-01T00:00:00Z Symphytum officinale L. é uma planta herbácea, cujo nome popular mais conhecido no Brasil é confrei. Suas folhas são empregadas na fitoterapia como antiinflamatório, emoliente e anestésico suave de uso tópico. Com a finalidade de contribuir com a identificação da planta medicinal recomendada pela RDC N° 17/2000 - ANVISA, foram analisadas a morfologia externa e a anatomia da folha. O material foi fixado, seccionado à mão livre e corado ou submetido a testes histoquímicos. A folha é ovalado-oblonga ou lanceolada, com ápice agudo ou acuminado, base cuneada e margem levemente ondulada. A epiderme é uniestratificada, com estômatos anomocíticos, tricomas tectores unicelulares retos e glandulares capitados em ambas as faces. Tricomas tectores unicelulares unciformes ocorrem na superfície abaxial. Mesofilo dorsiventral, células com mucilagem, nervura central apresentando um a oito feixes vasculares colaterais, e pecíolo com vários feixes colaterais em arco aberto são observados. Symphytum officinale L. is a herbaceous species, commonly known as comfrey in Brazil. Its leaves are employed in phytotherapy as topical anti-inflammatory, emollient and mild anesthetics. Aiming to contribute to the medicinal plant identification, which was recommended by RDC N° 17/2000 - ANVISA, the leaf external morphology and anatomy were carried out. The material was fixed, free-hand sectioned, either stained or undergone histochemical tests. The leaf is ovate-oblong or lanceolate, with acute or acuminate apex, cuneate base and slightly wavy margin. The epidermis is uniseriate, having anomocytic stomata, straight unicellular non-glandular trichomes and capitate glandular ones on both sides. Unciform unicellular non-glandular trichomes occur on the lower surface. Dorsiventral mesophyll, cells containing mucilage, midrib showing from one to eight collateral bundles, and petiole having various collateral bundles distributed as an open arc are observed. Fernandes, Carlos A. D. Nakashima, Tomoe Serra, Gil E. http://sedici.unlp.edu.ar:80/handle/10915/6664 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Articulo New contributions for the study of the crude galactomannan extracted from the seeds of Senna spectabilis DC Acta Farmacéutica Bonaerense; vol. 23, no. 3 A galactomanana bruta extraída de sementes de Senna spectabilis DC, árvore nativa da região Nordeste do Brasil pertencente à família Leguminosae, foi estudada quanto ao seu rendimento, composição e razão manose:galactose. Ela foi obtida por meio de uma única extração aquosa, à 75 °C por 24 h, a partir de um lote de sementes secas, moídas e pré-tratadas por maceração dinâmica usando quatro solventes em série. A galactomanana bruta apresentou um rendimento em peso (base seca) de 31,6%. São apresentados ainda, os teores de carboidratos totais, proteínas, ácidos urônicos, cinzas e umidade, além da composição em monossacarídeos e da razão manose:galactose. É fornecido também o teor de proteína residual presente na galactomanana bruta após um processo drástico de desproteinização. Considerando-se características como rendimento, composição e razão manose:galactose, concluí-se que a semente pode ser considerada como uma fonte promissora de obtenção de galactomanana para fins industriais.; The crude galactomannan extracted from the seeds of Senna spectabilis DC, a native tree from the northeast of Brazil belonging to the Leguminosae family, was study with regard to the yield, composition and manose to galactose ratio. The crude polysaccharide was extracted with water, at 75 °C for 24 h, from a sample of dry and milled seeds which were submitted a pretreatment by dynamic maceration using four solvents in series. The crude galactomannan show a weight yield (dry-weight basis) of 31,6%. The monosaccharide composition and the ratio of manose to galactose are give. It's also give the content of total carbohydrate, protein, uronic acid, ash and moisture, and the content of protein remained on the crude galactomannan after a drastic deproteinization. With regards to the yield, composition and manose to galactose ratio of the crude galactomannan, the seeds can be considered as a potential source of galactomannan for the industry.

2004-01-01T00:00:00Z A galactomanana bruta extraída de sementes de Senna spectabilis DC, árvore nativa da região Nordeste do Brasil pertencente à família Leguminosae, foi estudada quanto ao seu rendimento, composição e razão manose:galactose. Ela foi obtida por meio de uma única extração aquosa, à 75 °C por 24 h, a partir de um lote de sementes secas, moídas e pré-tratadas por maceração dinâmica usando quatro solventes em série. A galactomanana bruta apresentou um rendimento em peso (base seca) de 31,6%. São apresentados ainda, os teores de carboidratos totais, proteínas, ácidos urônicos, cinzas e umidade, além da composição em monossacarídeos e da razão manose:galactose. É fornecido também o teor de proteína residual presente na galactomanana bruta após um processo drástico de desproteinização. Considerando-se características como rendimento, composição e razão manose:galactose, concluí-se que a semente pode ser considerada como uma fonte promissora de obtenção de galactomanana para fins industriais. The crude galactomannan extracted from the seeds of Senna spectabilis DC, a native tree from the northeast of Brazil belonging to the Leguminosae family, was study with regard to the yield, composition and manose to galactose ratio. The crude polysaccharide was extracted with water, at 75 °C for 24 h, from a sample of dry and milled seeds which were submitted a pretreatment by dynamic maceration using four solvents in series. The crude galactomannan show a weight yield (dry-weight basis) of 31,6%. The monosaccharide composition and the ratio of manose to galactose are give. It's also give the content of total carbohydrate, protein, uronic acid, ash and moisture, and the content of protein remained on the crude galactomannan after a drastic deproteinization. With regards to the yield, composition and manose to galactose ratio of the crude galactomannan, the seeds can be considered as a potential source of galactomannan for the industry. Caldas Días Costa, Maria do Carmo Santos Aguiar, Jaciana dos Carneiro do Nascimento, Silene http://sedici.unlp.edu.ar:80/handle/10915/6663 2018-04-27T18:02:09Z 2004-01-01T00:00:00Z

Articulo Citotoxic activity of crude extracts of Lippia alba (Mill.) N.E. Brown (Verbenaceae) Acta Farmacéutica Bonaerense; vol. 23, no. 3 Extratos brutos da raiz, caule e folhas de Lippia alba (Mill.) N.E. Brown (Verbenaceae), foram testados frente às células NCI-H292 (obtidas de carcinoma mucoepidermoide de pulmão humano) e células HEp-2 (derivadas de carcinoma epidermóide de laringe). O extrato etanólico da folha mostrou-se mais citotóxico frente às células HEp-2 com CI₅₀ igual 8,17 µg/mL. Para às células NCI-H292 observou-se que o extrato clorofórmico da raiz foi o mais citotóxico, com CI₅₀ igual 4,64 µg/mL. Os constituintes com maiores ações antineoplásica estão presentes, principalmente, na raiz e folhas da espécie.; Crude extracts of Lippia alba (Mill.) N. E. Brown (Verbenaceae) roots, stem and leaves, were tested against cells lines of the pulmonary mucoepidermoid human carcinoma (NCI-H292) and cells lines derived from an epidermoid carcinoma of the human larynx (HEp-2). Ethanolic extract of the leaves showed more citotoxicity against HEp-2 cells with CI₅₀ = 8.17 µg/mL. In the case of NCI-H292 cells the chloroformic extract of the root was the most citotoxic, with CI₅₀ = 4.64 µg/mL. The constituents with higher antineoplasic action are present mostly in the root and leaves of the studied species.

2004-01-01T00:00:00Z Extratos brutos da raiz, caule e folhas de Lippia alba (Mill.) N.E. Brown (Verbenaceae), foram testados frente às células NCI-H292 (obtidas de carcinoma mucoepidermoide de pulmão humano) e células HEp-2 (derivadas de carcinoma epidermóide de laringe). O extrato etanólico da folha mostrou-se mais citotóxico frente às células HEp-2 com CI₅₀ igual 8,17 µg/mL. Para às células NCI-H292 observou-se que o extrato clorofórmico da raiz foi o mais citotóxico, com CI₅₀ igual 4,64 µg/mL. Os constituintes com maiores ações antineoplásica estão presentes, principalmente, na raiz e folhas da espécie. Crude extracts of Lippia alba (Mill.) N. E. Brown (Verbenaceae) roots, stem and leaves, were tested against cells lines of the pulmonary mucoepidermoid human carcinoma (NCI-H292) and cells lines derived from an epidermoid carcinoma of the human larynx (HEp-2). Ethanolic extract of the leaves showed more citotoxicity against HEp-2 cells with CI₅₀ = 8.17 µg/mL. In the case of NCI-H292 cells the chloroformic extract of the root was the most citotoxic, with CI₅₀ = 4.64 µg/mL. The constituents with higher antineoplasic action are present mostly in the root and leaves of the studied species. Oliveira, Silvânia Albuquerque, Mónica C. P. A. Pitta, Maira G. R. Malagüeño, Elisabeth Santana, José V. Lima, María do Carmo A. Pitta, Iván R. Galdino, Suely L. http://sedici.unlp.edu.ar:80/handle/10915/6662 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Articulo Behavior of Schistosoma mansoni adult worms maintained in vitro against Imidazolidinone derivatives Acta Farmacéutica Bonaerense; vol. 23, no. 3 Doenças tropicais afetam centenas de milhões de pessoas impedindo o desenvolvimento social e econômico nos países mais pobres. Ferramentas (por exemplo drogas, vacinas, diagnósticos) e estratégias para controlar as doenças tropicais freqüentemente não surtem efeitos devido a dificuldades na implementação ou esbarram no desenvolvimento de resistência de droga. Na procura de novos compostos esquistossomicidas, o trabalho presente descreve a síntese, estrutura e atividade esquistossomicida dos quimioterápicos (Z)-3-benzil-5-benzilideno-imidazolidina-2,4-dionas, (Z)-3-benzil-5-benzilideno-2-tioxo-imidazolidin- 4-onas e (Z)-5-benzilideno-3-(2-oxo-2-fenil-etil)-2-tioxo-imidazolidin-4-onas apresentando substituintes diferentes em posição para dos anéis aromáticos. Este estudo mostrou que entre os derivados testados o composto (Z)-3-(4-cloro-benzil)-5-(4-nitro-benzilideno)-imidazolidina-2,4-diona, apresentando um substituinte nitro no radical benzilideno, foi o que apresentou melhor atividade esquistossomicida in vitro.; Tropical diseases affect hundreds of millions people, most of whom are poor. They impede social and economic development in the poorest countries, and cause untold suffering to those affected. Tools (e.g. drugs, vaccines, diagnostics) and strategies to control tropical diseases often fail due to difficulties in implementation or development of drug resistance, for example. Research is needed to generate new knowledge, and to develop new and improved tools, methods and strategies for disease control. In the search for compounds with schistosomicidal activity, the present work describes the synthesis, structural and chemotherapeutic investigations of (Z)-3-benzyl-5-benzylidene-imidazolidine-2,4-dione or (Z)-3-benzyl-5-benzylidene-2-thioxo-imidazolidin-4-one or (Z)-5-benzylidene-3-(2-oxo-2-phenyl-ethyl)-2-thioxo-imidazolidin-4-one derivatives with different substituents in position para of the phenyl rings. This study clearly showed that among the tested compounds the (Z)-3-(4-cloro-benzyl)-5-(4-nitro-benzylidene)-imidazolidine-2,4-dione, a nitro derivative on benzylidene, was more active than chloro, bromo and unsubstituted compounds in producing mortality of Schistosoma mansoni adult worms maintained in vitro.

2004-01-01T00:00:00Z Doenças tropicais afetam centenas de milhões de pessoas impedindo o desenvolvimento social e econômico nos países mais pobres. Ferramentas (por exemplo drogas, vacinas, diagnósticos) e estratégias para controlar as doenças tropicais freqüentemente não surtem efeitos devido a dificuldades na implementação ou esbarram no desenvolvimento de resistência de droga. Na procura de novos compostos esquistossomicidas, o trabalho presente descreve a síntese, estrutura e atividade esquistossomicida dos quimioterápicos (Z)-3-benzil-5-benzilideno-imidazolidina-2,4-dionas, (Z)-3-benzil-5-benzilideno-2-tioxo-imidazolidin- 4-onas e (Z)-5-benzilideno-3-(2-oxo-2-fenil-etil)-2-tioxo-imidazolidin-4-onas apresentando substituintes diferentes em posição para dos anéis aromáticos. Este estudo mostrou que entre os derivados testados o composto (Z)-3-(4-cloro-benzil)-5-(4-nitro-benzilideno)-imidazolidina-2,4-diona, apresentando um substituinte nitro no radical benzilideno, foi o que apresentou melhor atividade esquistossomicida in vitro. Tropical diseases affect hundreds of millions people, most of whom are poor. They impede social and economic development in the poorest countries, and cause untold suffering to those affected. Tools (e.g. drugs, vaccines, diagnostics) and strategies to control tropical diseases often fail due to difficulties in implementation or development of drug resistance, for example. Research is needed to generate new knowledge, and to develop new and improved tools, methods and strategies for disease control. In the search for compounds with schistosomicidal activity, the present work describes the synthesis, structural and chemotherapeutic investigations of (Z)-3-benzyl-5-benzylidene-imidazolidine-2,4-dione or (Z)-3-benzyl-5-benzylidene-2-thioxo-imidazolidin-4-one or (Z)-5-benzylidene-3-(2-oxo-2-phenyl-ethyl)-2-thioxo-imidazolidin-4-one derivatives with different substituents in position para of the phenyl rings. This study clearly showed that among the tested compounds the (Z)-3-(4-cloro-benzyl)-5-(4-nitro-benzylidene)-imidazolidine-2,4-dione, a nitro derivative on benzylidene, was more active than chloro, bromo and unsubstituted compounds in producing mortality of Schistosoma mansoni adult worms maintained in vitro. Wagner, Claudia C. Barán, Enrique José http://sedici.unlp.edu.ar:80/handle/10915/6661 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Articulo Spectroscopic and magnetic behaviour of the copper (II) complex of L-tryptophan Acta Farmacéutica Bonaerense; vol. 23, no. 3 Un complejo de cobre (II) con el amino ácido L-triptofano, de estequiometría Cu(Trp)₂, fue obtenido de solución acuosa. Sus características estructurales fueron deducidas en base a un pormenorizado análisis de sus espectros de infrarrojo, Raman y de absorción electrónica, complementado con medidas de susceptibilidad magnética en el intervalo de temperaturas entre 2 y 300 K. El centro metálico presenta un entorno CuN₂O₄ octaédrico distorsionado con una disposición trans de los amino ácidos en el plano ecuatorial, involucrando los grupos amino y carboxilato terminales. La esfera de coordinación se complementa con dos enlaces Cu-O axiales más largos, involucrando átomos de oxígeno "libres" de grupos carboxilato de unidades complejas vecinas.; A copper(II) complex of the amino acid L-tryptophan, of stroichiometry Cu(Trp)₂, was obtained from aqueous solution. Its structural characteristics were deduced from the careful analysis of infrared, Raman and electronic absorption spectra, complemented with magnetic susceptibility measurements in the temperature range between 2 and 300 K. The metal center presents a distorted octahedral CuN₂O₄ environment with a trans arrangement of the amino acids in the equatorial plane, involving the terminal amino and carboxylate groups. The coordination sphere is complemented with two longer apical Cu-O bonds involving "free" O-carboxylato atoms of neighboring complex moieties.

2004-01-01T00:00:00Z Un complejo de cobre (II) con el amino ácido L-triptofano, de estequiometría Cu(Trp)₂, fue obtenido de solución acuosa. Sus características estructurales fueron deducidas en base a un pormenorizado análisis de sus espectros de infrarrojo, Raman y de absorción electrónica, complementado con medidas de susceptibilidad magnética en el intervalo de temperaturas entre 2 y 300 K. El centro metálico presenta un entorno CuN₂O₄ octaédrico distorsionado con una disposición trans de los amino ácidos en el plano ecuatorial, involucrando los grupos amino y carboxilato terminales. La esfera de coordinación se complementa con dos enlaces Cu-O axiales más largos, involucrando átomos de oxígeno "libres" de grupos carboxilato de unidades complejas vecinas. A copper(II) complex of the amino acid L-tryptophan, of stroichiometry Cu(Trp)₂, was obtained from aqueous solution. Its structural characteristics were deduced from the careful analysis of infrared, Raman and electronic absorption spectra, complemented with magnetic susceptibility measurements in the temperature range between 2 and 300 K. The metal center presents a distorted octahedral CuN₂O₄ environment with a trans arrangement of the amino acids in the equatorial plane, involving the terminal amino and carboxylate groups. The coordination sphere is complemented with two longer apical Cu-O bonds involving "free" O-carboxylato atoms of neighboring complex moieties. Rodrigues de Freitas, André Bochnia Albuquerque Cavalcanti, Osvaldo http://sedici.unlp.edu.ar:80/handle/10915/6660 2013-10-17T12:02:29Z 2004-01-01T00:00:00Z

Articulo Guar Gum as an ingredient in hydrophilic matrix: swelling index evaluation Acta Farmacéutica Bonaerense; vol. 23, no. 3 Formulações de matrizes hidrofílicas para administração oral usando polissacarídeos como transportadores para libertação modificada de fármacos têm sido amplamente investigadas. No presente estudo, avaliamos os diferentes valores de hidratação das matrizes hidrofílicas contendo hydroxipropilmetil celulose (HPMC) associada à goma guar em duas diferentes concentrações. Foram utilizados como fluídos de hidratação a água destilada, meio de simulação do fluído gástrico (pH 1,2) e simulação do fluído intestinal (pH 7,4). A captação gradual dos fluídos produziu intumescimento do sistema matricial, desenvolvendo superfície mucilaginosa a qual reteve o ingresso de líquidos ao interior do sistema. Este fenômeno tem permitido retardar e gerenciar o mecanismo de libertação de fármacos. As matrizes investigadas não apresentaram diferença significativa na captação de líquidos nos diferentes meios de hidratação utilizados. O aumento do índice de intumescimento foi proporcional e dependente do aumento da concentração da goma guar utilizada nas formulações. Os resultados obtidos sugerem que a goma guar, quando associada ao HPMC, apresenta influência no fenômeno de hidratação em sistemas matriciais hidrofílicos.; Hydrophilic matrices for oral administration using polysaccharides as carriers for modified release of drugs have been widely investigated. In the present study, we evaluated the different values of hydration of hydrophilic matrices including hydroxypropylmethyl cellulose (HPMC) associated with guar gum in two different concentrations. Distilled water and artificial gastric fluid (pH 1.2), and artificial intestinal fluid (pH 7.4), were used as hydration medium. The gradual uptake of these fluids produced a swelling of the matricial systems that developed a gel layers on the surface of the hydrophilic matrices, through which the drug has to pass by dissolution and diffusion. This phenomena has allowed the delay and control of the mechanism of drug release. The hydrophilic matrices investigated didn?t present significant differences in the intake of liquids in the different hydration means used. The increase in the swelling index was proportional and dependent on the increase of the guar gum concentration used in the formulations. The data suggest that the gum guar, when associated to the HPMC, shows an influence in the hydration phenomena in hydrophilic matrices systems.

2004-01-01T00:00:00Z Formulações de matrizes hidrofílicas para administração oral usando polissacarídeos como transportadores para libertação modificada de fármacos têm sido amplamente investigadas. No presente estudo, avaliamos os diferentes valores de hidratação das matrizes hidrofílicas contendo hydroxipropilmetil celulose (HPMC) associada à goma guar em duas diferentes concentrações. Foram utilizados como fluídos de hidratação a água destilada, meio de simulação do fluído gástrico (pH 1,2) e simulação do fluído intestinal (pH 7,4). A captação gradual dos fluídos produziu intumescimento do sistema matricial, desenvolvendo superfície mucilaginosa a qual reteve o ingresso de líquidos ao interior do sistema. Este fenômeno tem permitido retardar e gerenciar o mecanismo de libertação de fármacos. As matrizes investigadas não apresentaram diferença significativa na captação de líquidos nos diferentes meios de hidratação utilizados. O aumento do índice de intumescimento foi proporcional e dependente do aumento da concentração da goma guar utilizada nas formulações. Os resultados obtidos sugerem que a goma guar, quando associada ao HPMC, apresenta influência no fenômeno de hidratação em sistemas matriciais hidrofílicos. Hydrophilic matrices for oral administration using polysaccharides as carriers for modified release of drugs have been widely investigated. In the present study, we evaluated the different values of hydration of hydrophilic matrices including hydroxypropylmethyl cellulose (HPMC) associated with guar gum in two different concentrations. Distilled water and artificial gastric fluid (pH 1.2), and artificial intestinal fluid (pH 7.4), were used as hydration medium. The gradual uptake of these fluids produced a swelling of the matricial systems that developed a gel layers on the surface of the hydrophilic matrices, through which the drug has to pass by dissolution and diffusion. This phenomena has allowed the delay and control of the mechanism of drug release. The hydrophilic matrices investigated didn?t present significant differences in the intake of liquids in the different hydration means used. The increase in the swelling index was proportional and dependent on the increase of the guar gum concentration used in the formulations. The data suggest that the gum guar, when associated to the HPMC, shows an influence in the hydration phenomena in hydrophilic matrices systems.