http://sedici.unlp.edu.ar:80/handle/10915/212 2026-11-15T11:06:51Z 2026-11-15T11:06:51Z Lyra, Magalí A. M. de Soares Sobrinho, José L. Brasileiro, Mariana T. La Roca, Mónica F. de Barraza, Javier A. Viana Osnir de Sá Rolim Neto, Pedró José http://sedici.unlp.edu.ar:80/handle/10915/7550 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Revision Hydrophilic matrix And mucoadhesive systems for drug controlled release Latin American Journal of Pharmacy; vol. 26, no. 5 Os sistemas matriciais são de fácil obtenção e custo reduzido frente a outros sistemas de liberação controlada de fármacos, constituindo-se uma alternativa simples no desenvolvimento de formulações eficazes. Dentre os sistemas matriciais os sistemas constituídos por matrizes hidrofílicas biodegradáveis são os mais difundidos. Diversos polímeros hidrofílicos são aplicados a obtenção de formas farmacêuticas bioadesivas, em sua grande maioria mucoadesivas. A interação entre o polímero e a membrana mucosa é regida por diversos enlaces químicos e a elucidação do princípio da mucoadesão é objetivada por meio do estudo da teoria de adesão. Este trabalho objetivou explorar os diversos sistemas matriciais para liberação controlada de fármacos, suas aplicações mais recentes, tecnologia de obtenção, vantagens e desvantagens.; The matrix systems are more easily obtained and have reduced cost in comparison to others systems of drug-controlled release, consisting as a simple alternative in the development of efficient formulations. Amongst the matrix systems the systems consisting of biodegradable hydrophilic matrix are the most widely used. Diverse hydrophilic polymers are applied to the attainment of bioadhesive pharmaceutical forms, the great majority being mucoadhesives. The interaction between polymer and the mucous membrane is conducted by diverse chemical forces and to explain the principle of the mucoadhesion the study of the adhesion theory is objectified. The aim of this work was to explore the diverse matrix systems of drug controlled release, its more recent applications, technology of attainment, advantages and disadvantages.

2007-01-01T00:00:00Z Os sistemas matriciais são de fácil obtenção e custo reduzido frente a outros sistemas de liberação controlada de fármacos, constituindo-se uma alternativa simples no desenvolvimento de formulações eficazes. Dentre os sistemas matriciais os sistemas constituídos por matrizes hidrofílicas biodegradáveis são os mais difundidos. Diversos polímeros hidrofílicos são aplicados a obtenção de formas farmacêuticas bioadesivas, em sua grande maioria mucoadesivas. A interação entre o polímero e a membrana mucosa é regida por diversos enlaces químicos e a elucidação do princípio da mucoadesão é objetivada por meio do estudo da teoria de adesão. Este trabalho objetivou explorar os diversos sistemas matriciais para liberação controlada de fármacos, suas aplicações mais recentes, tecnologia de obtenção, vantagens e desvantagens. The matrix systems are more easily obtained and have reduced cost in comparison to others systems of drug-controlled release, consisting as a simple alternative in the development of efficient formulations. Amongst the matrix systems the systems consisting of biodegradable hydrophilic matrix are the most widely used. Diverse hydrophilic polymers are applied to the attainment of bioadhesive pharmaceutical forms, the great majority being mucoadhesives. The interaction between polymer and the mucous membrane is conducted by diverse chemical forces and to explain the principle of the mucoadhesion the study of the adhesion theory is objectified. The aim of this work was to explore the diverse matrix systems of drug controlled release, its more recent applications, technology of attainment, advantages and disadvantages. Blatt, Carine Raquel Rocha Farías, Mareni http://sedici.unlp.edu.ar:80/handle/10915/7549 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Articulo Exceptional Drugs Program Diagnostic of the Santa Catarina State - Brazil Latin American Journal of Pharmacy; vol. 26, no. 5 Este estudo tem como objetivo realizar um diagnóstico do Programa de Medicamentos Excep- cionais do Estado de Santa Catarina - Brasil, com ênfase no local onde ocorre a dispensação dos medica- mentos, denominados Centros de Custo. Foi realizado um estudo de caso em 68 Centros de Custo. Além disso, foram levantados dados relacionados aos gastos com a aquisição e o consumo. No período de 1999 a 2004 ocorreu um crescimento exponencial do Programa. Em 2004, apenas dez medicamentos somaram 55% dos gastos e corresponderam a menos de 22% dos processos. A análise da dispensação dos medica- mentos demonstrou deficiências importantes quanto à infra-estrutura de pessoal, condições de armazena- mento e de atendimento. O crescente valor financeiro aplicado e o custo unitário elevado de alguns medi- camentos apontam a necessidade de organização e estruturação deste serviço, bem como do acompanha- mento farmacoterapêutico dos pacientes possibilitando a melhoria da qualidade da assistência farmacêuti- ca e a racionalização do gasto em saúde.; The aim of this study is evaluat the Exceptional Drugs Program of Santa Catarina State - Brazil, with emphasis on the place of drug distribution, named Cost Centers. A case study was done in 68 Cost Centers. Data related to the costs of acquisi- tion and use were alson assissed. In the period between 1999 and 2004 the Program grew exponentially. In 2004, only ten drugs were responsible for 55% of all costs and corresponded to less than 22% of cases. The analysis of the centers responsables for drug distribuition demonstrated important deficiencies related to infrastructure, at- tendance and storage conditions. The increase in investment and the high unitary cost of some drugs mean that this service needs to be well organized and structured. There also needs to be drug care follow-up of patients al- low for the improvement of the quality of the pharmaceutical assistance and the rationalization of health expendi- tures.

2007-01-01T00:00:00Z Este estudo tem como objetivo realizar um diagnóstico do Programa de Medicamentos Excep- cionais do Estado de Santa Catarina - Brasil, com ênfase no local onde ocorre a dispensação dos medica- mentos, denominados Centros de Custo. Foi realizado um estudo de caso em 68 Centros de Custo. Além disso, foram levantados dados relacionados aos gastos com a aquisição e o consumo. No período de 1999 a 2004 ocorreu um crescimento exponencial do Programa. Em 2004, apenas dez medicamentos somaram 55% dos gastos e corresponderam a menos de 22% dos processos. A análise da dispensação dos medica- mentos demonstrou deficiências importantes quanto à infra-estrutura de pessoal, condições de armazena- mento e de atendimento. O crescente valor financeiro aplicado e o custo unitário elevado de alguns medi- camentos apontam a necessidade de organização e estruturação deste serviço, bem como do acompanha- mento farmacoterapêutico dos pacientes possibilitando a melhoria da qualidade da assistência farmacêuti- ca e a racionalização do gasto em saúde. The aim of this study is evaluat the Exceptional Drugs Program of Santa Catarina State - Brazil, with emphasis on the place of drug distribution, named Cost Centers. A case study was done in 68 Cost Centers. Data related to the costs of acquisi- tion and use were alson assissed. In the period between 1999 and 2004 the Program grew exponentially. In 2004, only ten drugs were responsible for 55% of all costs and corresponded to less than 22% of cases. The analysis of the centers responsables for drug distribuition demonstrated important deficiencies related to infrastructure, at- tendance and storage conditions. The increase in investment and the high unitary cost of some drugs mean that this service needs to be well organized and structured. There also needs to be drug care follow-up of patients al- low for the improvement of the quality of the pharmaceutical assistance and the rationalization of health expendi- tures. Pinto, Claudinéia A. S. O. Green, Denise Rolim Baby, André Ruas, Gabriele W. Kaneko, Telma Mary Marana, Sandro R. Velasco, María Valéria R. http://sedici.unlp.edu.ar:80/handle/10915/7548 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Articulo Determination of papain activity in topical dosage forms: single laboratory validation assay Latin American Journal of Pharmacy; vol. 26, no. 5 La papaína es una enzima proteolítica usada en formulaciones tópicas para el tratamiento de quemaduras, heridas, cicatrices, exfoliación de la piel mediante depilado, así como agente depilatorio progresivo y para incrementar la absorción cutánea. El presente trabajo tiene como finalidad validar una metodología que cuantifique la actividad de la papaína en formas farmacéuticas semisólidas. Para la validación del método fueron considerados los siguientes parámetros: linealidad, especificidad, límite de detección, cuantificación, precisión y exactitud. Benziloxicarbonil-fenilialanina-arginina 7-amido 4-metilcumarina fue usada como sustrato para el análisis de la actividad enzimática de la papaína, la cual fue determinada por detección espectrofluorormétrica de la 7- amido-4-metilcumarina adherida a microplacas. El método fue validado de acuerdo con los siguientes resultados experimentales: coeficiente de correlación (r = 0,9974); Límite de detección (LOD = 0,040 USP.mL–1 ), límite de cuantificación (LOQ = 0,12 USP.mL–1 ), precisión (RSD % = 2,7 a 5,0), y exactitud (E% = 95,3 a 96,7), demostrando además especificidad para el ensayo realizado en las formulaciones tópicas, siendo este un método simple, rápido y con una adecuada sensibilidad.; Papain is a proteolytic enzyme used in topical formulations for peeling and treatment of burns, wounds and scars. It is also used as progressive depilatory agent and as cutaneous absorption enhancer. This work was aimed at validating a spectrofluorimetic method to quantify the activity of papain in semisolid pharmaceutical dosage forms. Benzyloxycarbonyl-phenylalanyl-arginine 7-amido-4-methylcoumarin was used as substrate in assays performed in microplates. The production of 7-amido-4-methylcoumarin (7-MCA) over different periods of time was used to quantify the concentration of papain in pharmaceutical forms. This method presented a linear relationship between papain concentration and rate of 7-MCA production (R = 0.9974). Besides that, the method presented an estimated detection limit (LOD) of 0.040 USP.mL–1 , an estimated quantification limit (LOQ) of 0.12 USP.mL–1 , a precision (RSD%) ranging from 2.7 to 5.0 and an accuracy (E%) of 95.3 to 96.7. Components of the tested topical formulation did not interfere in the papain detection. Finally, the method is simple and fast.

2007-01-01T00:00:00Z La papaína es una enzima proteolítica usada en formulaciones tópicas para el tratamiento de quemaduras, heridas, cicatrices, exfoliación de la piel mediante depilado, así como agente depilatorio progresivo y para incrementar la absorción cutánea. El presente trabajo tiene como finalidad validar una metodología que cuantifique la actividad de la papaína en formas farmacéuticas semisólidas. Para la validación del método fueron considerados los siguientes parámetros: linealidad, especificidad, límite de detección, cuantificación, precisión y exactitud. Benziloxicarbonil-fenilialanina-arginina 7-amido 4-metilcumarina fue usada como sustrato para el análisis de la actividad enzimática de la papaína, la cual fue determinada por detección espectrofluorormétrica de la 7- amido-4-metilcumarina adherida a microplacas. El método fue validado de acuerdo con los siguientes resultados experimentales: coeficiente de correlación (r = 0,9974); Límite de detección (LOD = 0,040 USP.mL–1 ), límite de cuantificación (LOQ = 0,12 USP.mL–1 ), precisión (RSD % = 2,7 a 5,0), y exactitud (E% = 95,3 a 96,7), demostrando además especificidad para el ensayo realizado en las formulaciones tópicas, siendo este un método simple, rápido y con una adecuada sensibilidad. Papain is a proteolytic enzyme used in topical formulations for peeling and treatment of burns, wounds and scars. It is also used as progressive depilatory agent and as cutaneous absorption enhancer. This work was aimed at validating a spectrofluorimetic method to quantify the activity of papain in semisolid pharmaceutical dosage forms. Benzyloxycarbonyl-phenylalanyl-arginine 7-amido-4-methylcoumarin was used as substrate in assays performed in microplates. The production of 7-amido-4-methylcoumarin (7-MCA) over different periods of time was used to quantify the concentration of papain in pharmaceutical forms. This method presented a linear relationship between papain concentration and rate of 7-MCA production (R = 0.9974). Besides that, the method presented an estimated detection limit (LOD) of 0.040 USP.mL–1 , an estimated quantification limit (LOQ) of 0.12 USP.mL–1 , a precision (RSD%) ranging from 2.7 to 5.0 and an accuracy (E%) of 95.3 to 96.7. Components of the tested topical formulation did not interfere in the papain detection. Finally, the method is simple and fast. Weich, Anelise Carvalho de Oliveira, Daniele Melo, Janine de Goebel, Karin Rolim Clarice M. B. http://sedici.unlp.edu.ar:80/handle/10915/7547 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Articulo Validation of UV spectrophotometric and HPLC methods for quantitative determination of Atenolol in pharmaceutical preparations Latin American Journal of Pharmacy; vol. 26, no. 5 Se ha desarrollado y validado un método de cromatografía líquida y espectrofotometría ultravioleta para la determinación de comprimidos de atenolol. La fase móvil empleada fue buffer acetato de amonio 10 mM (pH 7.0) y acetonitrilo (80:20 v/v). Las muestras se inyectaron en una columna Purospher RP-18 (250 mm x 4,6 mm, 5 mm), con una velocidad de flujo de 0,8 ml.min–1 Las muestras . se detectaron a 275 nm. El ensayo resultó lineal en el rango de concentración de 125 a 375 μg.ml–1 con un coeficiente de la correlación (r = 0,9999) muy significativo para el método. La exactitud del método fue de 99,8 %. El método por espectrofotometría ultravioleta se realizó a 226 nm y las muestras se preapararon en una solución de acetato de sodio. La linearidad fue adecuada (r = 0,9986). Los métodos propuestos son simples, rápidos, precisos, exactos y sensibles y por lo tanto pueden usarse para la rutina de control de calidad en productos farmacéuticos.; A rapid and sensitive RP-HPLC method with UV detection and UV spectrophotometric method for routine control of atenolol in tablets was developed. Chromatography was performed with mobile phase containing a mixture of 10 mM ammonium acetate buffer (pH 7.0) and acetonitrile (80:20 v/v). The samples were injected onto Purospher RP-18 (250 mm x 4.6 mm, 5 μm) column. The flow rate was 0.8 ml.min–1 The samples were detected at 275 nm. The assay was linear in range from 125 to 375 μg.ml–1 . with a correlation coefficient (r=0.9999) highly significant for the method. The accuracy was 99.80%. The UV spectrophotometric method was performed at 226 nm, and samples were prepared with a solution of sodium acetate. The linearity demonstrated a correlation coefficient of 0.9986. The proposed methods were simple, rapid, precise, accurate and sensitive, and can be used for the routine of the quality control in pharmaceuticals.

2007-01-01T00:00:00Z Se ha desarrollado y validado un método de cromatografía líquida y espectrofotometría ultravioleta para la determinación de comprimidos de atenolol. La fase móvil empleada fue buffer acetato de amonio 10 mM (pH 7.0) y acetonitrilo (80:20 v/v). Las muestras se inyectaron en una columna Purospher RP-18 (250 mm x 4,6 mm, 5 mm), con una velocidad de flujo de 0,8 ml.min–1 Las muestras . se detectaron a 275 nm. El ensayo resultó lineal en el rango de concentración de 125 a 375 μg.ml–1 con un coeficiente de la correlación (r = 0,9999) muy significativo para el método. La exactitud del método fue de 99,8 %. El método por espectrofotometría ultravioleta se realizó a 226 nm y las muestras se preapararon en una solución de acetato de sodio. La linearidad fue adecuada (r = 0,9986). Los métodos propuestos son simples, rápidos, precisos, exactos y sensibles y por lo tanto pueden usarse para la rutina de control de calidad en productos farmacéuticos. A rapid and sensitive RP-HPLC method with UV detection and UV spectrophotometric method for routine control of atenolol in tablets was developed. Chromatography was performed with mobile phase containing a mixture of 10 mM ammonium acetate buffer (pH 7.0) and acetonitrile (80:20 v/v). The samples were injected onto Purospher RP-18 (250 mm x 4.6 mm, 5 μm) column. The flow rate was 0.8 ml.min–1 The samples were detected at 275 nm. The assay was linear in range from 125 to 375 μg.ml–1 . with a correlation coefficient (r=0.9999) highly significant for the method. The accuracy was 99.80%. The UV spectrophotometric method was performed at 226 nm, and samples were prepared with a solution of sodium acetate. The linearity demonstrated a correlation coefficient of 0.9986. The proposed methods were simple, rapid, precise, accurate and sensitive, and can be used for the routine of the quality control in pharmaceuticals. Luna Saucedo, Bárbara E. Rodríguez Rodríguez, Ana C. Quevedo Álvarez, Odalys Cabrera Rabí, Leila http://sedici.unlp.edu.ar:80/handle/10915/7546 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Articulo Heavy metals determination in a nutritional suplement from natural origin with antioxidant properties Latin American Journal of Pharmacy; vol. 26, no. 5 El uso de productos naturales como suplementos nutricionales y fármacos requiere conocer con exactitud el contenido de algunos metales tóxicos para el hombre, tales como Cd, Pb, As y Hg. El D-OO3 es un principio activo extraído de la cera de la caña de azúcar, constituido por una mezcla de ácidos alifáticos de alto peso molecular, con propiedades antioxidantes. En este trabajo se determinó la concentración de As, Cd, Pb y Hg en D-OO3 por Espectrometría de Absorción Atómica con llama, empleando en el procedimiento de digestión una mezcla de los ácidos: nítrico, sulfúrico y perclórico. En la determinación de As y Hg se emplearon las técnicas de Generación de Hidruros y Vapor Frío, respectivamente. La exactitud del procedimiento de digestión empleado para cada caso fue evaluada satisfactoriamente por el método de añadido de estándar y determinación de la recuperación, lográndose por cientos de recuperación entre 95 y 105%. Los límites de detección estimados para el As, Cd, Pb y Hg fueron de 0,002, 0,02, 0,30 y 0,01 ppm, respectivamente. Los contenidos de As, Cd, Pb y Hg en el D-OO3 se encontraron por debajo de estos límites, así como del establecido por la USP 27 Ed. para productos similares.; The use of natural products as nutritional suplements or drugs requires to know with accuracy the content of some toxic metals as Cd, Pb, As and Hg. The D-003 is a root material extracted from the sugar cane and composed by high molecular weight alifatic acids with antioxidant properties. In this work the concentration of As, Cd, Pb and Hg in D-OO3 by flame atomic absoption spectrometry was determined, employing a digestion procedure with nitric, sulfuric and percloric acids. The hydride generation technique was used to determine As and cold vapour method for the determination of Hg. The accuracy of the determination was evaluated by the standard additions method and the recovery was satisfactory between 95 and 105%. The detection limits were 0.002, 0.02, 0.30 y 0.01 ppm for As, Cd, Pb y Hg, respectively. The contents of As, Cd, Pb and Hg were under the limits of detection as well as the established limit of the 27 United States Pharmacopeia for similar products.

2007-01-01T00:00:00Z El uso de productos naturales como suplementos nutricionales y fármacos requiere conocer con exactitud el contenido de algunos metales tóxicos para el hombre, tales como Cd, Pb, As y Hg. El D-OO3 es un principio activo extraído de la cera de la caña de azúcar, constituido por una mezcla de ácidos alifáticos de alto peso molecular, con propiedades antioxidantes. En este trabajo se determinó la concentración de As, Cd, Pb y Hg en D-OO3 por Espectrometría de Absorción Atómica con llama, empleando en el procedimiento de digestión una mezcla de los ácidos: nítrico, sulfúrico y perclórico. En la determinación de As y Hg se emplearon las técnicas de Generación de Hidruros y Vapor Frío, respectivamente. La exactitud del procedimiento de digestión empleado para cada caso fue evaluada satisfactoriamente por el método de añadido de estándar y determinación de la recuperación, lográndose por cientos de recuperación entre 95 y 105%. Los límites de detección estimados para el As, Cd, Pb y Hg fueron de 0,002, 0,02, 0,30 y 0,01 ppm, respectivamente. Los contenidos de As, Cd, Pb y Hg en el D-OO3 se encontraron por debajo de estos límites, así como del establecido por la USP 27 Ed. para productos similares. The use of natural products as nutritional suplements or drugs requires to know with accuracy the content of some toxic metals as Cd, Pb, As and Hg. The D-003 is a root material extracted from the sugar cane and composed by high molecular weight alifatic acids with antioxidant properties. In this work the concentration of As, Cd, Pb and Hg in D-OO3 by flame atomic absoption spectrometry was determined, employing a digestion procedure with nitric, sulfuric and percloric acids. The hydride generation technique was used to determine As and cold vapour method for the determination of Hg. The accuracy of the determination was evaluated by the standard additions method and the recovery was satisfactory between 95 and 105%. The detection limits were 0.002, 0.02, 0.30 y 0.01 ppm for As, Cd, Pb y Hg, respectively. The contents of As, Cd, Pb and Hg were under the limits of detection as well as the established limit of the 27 United States Pharmacopeia for similar products. Venquiarutti Escarrone, Ana Laura Laporta, Luciane V. Santos, Marcos R. dos Friedrich, Milene Bittencourt, Celso F. http://sedici.unlp.edu.ar:80/handle/10915/7545 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Articulo Development and validation of analytical methodology by agar diffusion assay for determination of Ciclopirox olamine topical solution Latin American Journal of Pharmacy; vol. 26, no. 5 A freqüência de infecções fúngicas tem aumentado nas duas últimas décadas em decorrência do prolongamento da vida, em razão do avanço na tecnologia médica. Esta sobrevida ocasionou a manifestação de infecções fúngicas em novas proporções, o que aumentou o interesse pelo estudo dos fármacos antifúngicos. O ciclopirox olamina é um agente antifúngico com amplo espectro de ação, com atividades antiinflamatória e antibacteriana. Neste trabalho foi desenvolvido e validado método por difusão em ágar para quantificação de solução tópica de ciclopirox olamina, empregando Candida albicans ATCC 10231 como microrganismo teste. O método apresentou linearidade, precisão e exatidão adequadas.; Frequency of fungal infections has risen during the last decades due to the increasing human lifespan, because of the advance in the capacity of medical technology. This survival rate has brought about a manifestation of fungal infections at new proportions, which has raised the interest in studying anti-fungal drugs. Ciclopirox olamina is an anti-fungal agent with a wide spectrum of action, with anti-inflammatory and anti-bacterial activities. In this study, method by agar diffusion for the quantification of ciclopirox olamina as topical solution were developed and validated, employing Candida albicans ATCC 10231. The method presented adequate linearity, precision and accuracy.

2007-01-01T00:00:00Z A freqüência de infecções fúngicas tem aumentado nas duas últimas décadas em decorrência do prolongamento da vida, em razão do avanço na tecnologia médica. Esta sobrevida ocasionou a manifestação de infecções fúngicas em novas proporções, o que aumentou o interesse pelo estudo dos fármacos antifúngicos. O ciclopirox olamina é um agente antifúngico com amplo espectro de ação, com atividades antiinflamatória e antibacteriana. Neste trabalho foi desenvolvido e validado método por difusão em ágar para quantificação de solução tópica de ciclopirox olamina, empregando Candida albicans ATCC 10231 como microrganismo teste. O método apresentou linearidade, precisão e exatidão adequadas. Frequency of fungal infections has risen during the last decades due to the increasing human lifespan, because of the advance in the capacity of medical technology. This survival rate has brought about a manifestation of fungal infections at new proportions, which has raised the interest in studying anti-fungal drugs. Ciclopirox olamina is an anti-fungal agent with a wide spectrum of action, with anti-inflammatory and anti-bacterial activities. In this study, method by agar diffusion for the quantification of ciclopirox olamina as topical solution were developed and validated, employing Candida albicans ATCC 10231. The method presented adequate linearity, precision and accuracy. Silva Filho, Carlos R. da Kondo, Roberto T. O. Chignalia, Andréia Z. López, Karin V. Pereira, Valeria A. Carmona, María J. C. Auler Junior, José O. C. Santos, Silvia R. C. J. http://sedici.unlp.edu.ar:80/handle/10915/7544 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Articulo Quantification of Proprofol in small volumes of plasma using high-performance liquid chromatography with fluorescence detection Latin American Journal of Pharmacy; vol. 26, no. 5 Adaptamos un método por cromatografía líquida con detector de fluorescencia para la determinación de propofol en pequeños volúmenes de plasma usando una columna C18. Los picos fueron eluidos a los 21 y 12 min para propofol y timol (padrón interno), respectivamente, usando una fase móvil binaria y un flujo de 0,8 ml/min. El método validado presenta una sensibilidad de 0,05 2 mg/L, una linearidad 0,1 -10 mg/L, (r 0,9977), un erro sistemático de 8,2% y 7,0% (intra e interdiario), preci- sión 8,4% e 8,8% (intra y interdiario). Reportamos buena estabilidad , robustez y una recuperación de 93,9%. El método analítico fue validado por la cuantificación de propofol en plasma arterial (C :3,3 mg/L C : 0,2 max min mg/L) de un paciente recibiendo 1570 mg de propofol por infusión, en el periodo perioperatorio de la cirugía de revascularización al miocardio. La anestesia profunda expresada como ídice biespectral (BIS) fue 36 (32-40) en la cuarta hora y la hipnosis adecuada fue alcanzada antes del fin de la cirugía.; A liquid chromatographic method with fluorescence detection for determination of propofol in small volumes of plasma has been adapted to the conditions of the laboratory using a C18 column. Peaks were eluted at 21 min propofol and at 12 min thymol (internal standard) using a binary mobile phase at flow rate 0.8 mL/min. The method validated presents 0.05 mg/L sensitivity, 0.1 - 10 mg/L lineari- 2 ty, (r 0.9977), systematic error of 8.2 and 7.0% (intra- and interday accuracy), intra- and interday preci- sion (CV %: 8.4% and 8.8%). Recovery 93.9%, good stability and robustness were reported. The analyti- cal method was validated by the quantification of propofol in arterial plasma (C :3.3 mg/L C : max min 0.2mg/L) of one patient received 1570 mg infusion of propofol in the perioperative period of surgery of myocardial revascularization. Depth of anaesthesia expressed as bispectral index (BIS) was 36 (32-40) in th that period up to the 4 h, and an adequate hypnosis was obtained until the end of surgery.

2007-01-01T00:00:00Z Adaptamos un método por cromatografía líquida con detector de fluorescencia para la determinación de propofol en pequeños volúmenes de plasma usando una columna C18. Los picos fueron eluidos a los 21 y 12 min para propofol y timol (padrón interno), respectivamente, usando una fase móvil binaria y un flujo de 0,8 ml/min. El método validado presenta una sensibilidad de 0,05 2 mg/L, una linearidad 0,1 -10 mg/L, (r 0,9977), un erro sistemático de 8,2% y 7,0% (intra e interdiario), preci- sión 8,4% e 8,8% (intra y interdiario). Reportamos buena estabilidad , robustez y una recuperación de 93,9%. El método analítico fue validado por la cuantificación de propofol en plasma arterial (C :3,3 mg/L C : 0,2 max min mg/L) de un paciente recibiendo 1570 mg de propofol por infusión, en el periodo perioperatorio de la cirugía de revascularización al miocardio. La anestesia profunda expresada como ídice biespectral (BIS) fue 36 (32-40) en la cuarta hora y la hipnosis adecuada fue alcanzada antes del fin de la cirugía. A liquid chromatographic method with fluorescence detection for determination of propofol in small volumes of plasma has been adapted to the conditions of the laboratory using a C18 column. Peaks were eluted at 21 min propofol and at 12 min thymol (internal standard) using a binary mobile phase at flow rate 0.8 mL/min. The method validated presents 0.05 mg/L sensitivity, 0.1 - 10 mg/L lineari- 2 ty, (r 0.9977), systematic error of 8.2 and 7.0% (intra- and interday accuracy), intra- and interday preci- sion (CV %: 8.4% and 8.8%). Recovery 93.9%, good stability and robustness were reported. The analyti- cal method was validated by the quantification of propofol in arterial plasma (C :3.3 mg/L C : max min 0.2mg/L) of one patient received 1570 mg infusion of propofol in the perioperative period of surgery of myocardial revascularization. Depth of anaesthesia expressed as bispectral index (BIS) was 36 (32-40) in th that period up to the 4 h, and an adequate hypnosis was obtained until the end of surgery. Severino, Patrícia Zanchetta, Beatriz Franco, Luiz M. Paganelli, María Ondina Tamascia, Pollyana Chaud, Marco Vinicius http://sedici.unlp.edu.ar:80/handle/10915/7543 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Comunicacion Intestinal absorption and physical chemical stability in fluconazole extemporaneous preparations Latin American Journal of Pharmacy; vol. 26, no. 5 O Fluconazol (FCZ) é um agente antifúngico sintético do grupo azóis, com excelente biodisponibilidade nos fluidos corpóreos e utilizado para diversos tratamentos como histoplasmose, blastomicose, candidíase sendo considerado o fármaco de primeira escolha na maioria dos tipos de meningites fúngicas. O objetivo deste trabalho foi avaliar as propriedades físico-químicas da forma farmacêutica líquida e a absorção intestinal FCZ contido, nos medicamentos preparados extemporaneamente. A absorção intestinal do FCZ foi avaliada pelo método do segmento invertido e a estabilidade físico-química através da concentração de FCZ nas preparações extemporâneas. Não há, nenhuma garantia de qualidade no processo de preparação extemporânea, seja do ponto de vista físico, químico ou biofarmcêutico. As preparações extemporâneas foram acompanhadas durante 15 dias e mostraram-se fisicamente instáveis o que pode ter influenciado no perfil de absorção intestinal do FCZ.; Fluconazole (FCZ) is synthetic antimycotic agent from azois group, with excellent bioavailability in body fluids and used for many treatments such as histoplasmoses, blastomycosis, and candidiasis. FCZ is considered the first chosen drug in the majority types of fungal meningitis. The objective of this work was to evaluate the physical-chemical characteristics of the FCZ in liquid pharmaceutical form and FCZ intestinal absorption in aqueous dispersion, in extemporaneous preparation. The FCZ intestinal absorption was evaluated through inverted intestinal sac and of the physical chemical stability through FCZ concentration in extemporaneous preparations. There is no quality guarantee in the extemporaneous preparation process, in the physical, chemical or biopharmaceutical view. The extemporaneous preparation were evaluated for 15 days and proved to be physically unstable. This may have influenced the FCZ intestinal absorption profile.

2007-01-01T00:00:00Z O Fluconazol (FCZ) é um agente antifúngico sintético do grupo azóis, com excelente biodisponibilidade nos fluidos corpóreos e utilizado para diversos tratamentos como histoplasmose, blastomicose, candidíase sendo considerado o fármaco de primeira escolha na maioria dos tipos de meningites fúngicas. O objetivo deste trabalho foi avaliar as propriedades físico-químicas da forma farmacêutica líquida e a absorção intestinal FCZ contido, nos medicamentos preparados extemporaneamente. A absorção intestinal do FCZ foi avaliada pelo método do segmento invertido e a estabilidade físico-química através da concentração de FCZ nas preparações extemporâneas. Não há, nenhuma garantia de qualidade no processo de preparação extemporânea, seja do ponto de vista físico, químico ou biofarmcêutico. As preparações extemporâneas foram acompanhadas durante 15 dias e mostraram-se fisicamente instáveis o que pode ter influenciado no perfil de absorção intestinal do FCZ. Fluconazole (FCZ) is synthetic antimycotic agent from azois group, with excellent bioavailability in body fluids and used for many treatments such as histoplasmoses, blastomycosis, and candidiasis. FCZ is considered the first chosen drug in the majority types of fungal meningitis. The objective of this work was to evaluate the physical-chemical characteristics of the FCZ in liquid pharmaceutical form and FCZ intestinal absorption in aqueous dispersion, in extemporaneous preparation. The FCZ intestinal absorption was evaluated through inverted intestinal sac and of the physical chemical stability through FCZ concentration in extemporaneous preparations. There is no quality guarantee in the extemporaneous preparation process, in the physical, chemical or biopharmaceutical view. The extemporaneous preparation were evaluated for 15 days and proved to be physically unstable. This may have influenced the FCZ intestinal absorption profile. Nogueira, Marisa A. Díaz, Gaspar Stangarlin, José Renato http://sedici.unlp.edu.ar:80/handle/10915/7542 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Comunicacion Search of antibiotic metabolites from phytopathogenic fungi Latin American Journal of Pharmacy; vol. 26, no. 5 Los extractos de acetato de etilo y diclorometano de cultivos de los hongos fitopatógenos Fusarium solani, Colletotrichum musae, Cladosporium fulvum, Septoria lycopersici, Rhizoctonia solani y Alternaria solani mostraron actividad antibacteriana utilizando técnicas de bioautografia contra algunas bacterias multi-droga resistentes aisladas de pacientes del hospital universitario (HUOP-Unioeste).; Ethyl acetate and dichloromethane extracts from culture broths of Fusarium solani, Colletotrichum musae, Cladosporium fulvum, Septoria lycopersici, Rhizoctonia solani and Alternaria solani phytopathogenic fungi showed antibacterial activity by bioautography methods against bacteria isolated from patient of University Hospital (HUOP-Unioeste), some of them multi-drug resistant.

2007-01-01T00:00:00Z Los extractos de acetato de etilo y diclorometano de cultivos de los hongos fitopatógenos Fusarium solani, Colletotrichum musae, Cladosporium fulvum, Septoria lycopersici, Rhizoctonia solani y Alternaria solani mostraron actividad antibacteriana utilizando técnicas de bioautografia contra algunas bacterias multi-droga resistentes aisladas de pacientes del hospital universitario (HUOP-Unioeste). Ethyl acetate and dichloromethane extracts from culture broths of Fusarium solani, Colletotrichum musae, Cladosporium fulvum, Septoria lycopersici, Rhizoctonia solani and Alternaria solani phytopathogenic fungi showed antibacterial activity by bioautography methods against bacteria isolated from patient of University Hospital (HUOP-Unioeste), some of them multi-drug resistant. Rossi, Juliana C. N. Prophiro, Josiane S. Mendes, Anderson M. Kanis, Luiz A. Silva, Onilda S. http://sedici.unlp.edu.ar:80/handle/10915/7541 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Comunicacion Larvicidal effect of ethanolic extracts from Melia azedarach dried leaves and ripe fruits in Aedes albopictus larvae Latin American Journal of Pharmacy; vol. 26, no. 5 Neste trabalho determinou-se o efeito larvicida de extratos etanólicos de frutos maduros e folhas secas de Melia azedarach em Aedes alpopictus silvestres. Os bioensaios de letalidade foram realizados de acordo com as recomendações da Organização Mundial da Saúde. Não foi observada diferença significativa entre as concentrações letais determinadas entre o 3º e 4º estágios para os diferentes extratos, entretanto, as LC₉₀ e LC₉₅ demonstram a maior letalidade do extrato obtido de frutos maduros. Estes resultados sugerem que tanto os extratos de frutos maduros como de folhas secas de M. azedarach apresentam potencial para o desenvolvimento de larvicidas para o Ae. albopictus. inserted in small depressions, secretory ducts associated to the phloem and idioblasts containing calcium oxalate are reported as the mainly morpho-anatomical data for Baccharis coridifolia; Ethanolic extracts were obtained by fruits and leaves maceration and the solvent was removed in a rotary evaporator. The lethality bioassays (LC₅₀ , LC₉₀ and LC₉₅ ) were carried out according to the recommendations of the World Health Organization. Leaves and ripe fruits extracts were tested at doses ranging from 0.015 g% to 1,20 g% and 0,015 g% to 0,9 g% ,respectively, for the third and fourth instar larvae. Both extracts showed bioactivity in all doses and instar larvae tested, although ripe fruits extracts showed higher activity. Results indicate that these botanical extracts could be used as an alternative resource to control Ae. albopictus larvae.

2007-01-01T00:00:00Z Neste trabalho determinou-se o efeito larvicida de extratos etanólicos de frutos maduros e folhas secas de Melia azedarach em Aedes alpopictus silvestres. Os bioensaios de letalidade foram realizados de acordo com as recomendações da Organização Mundial da Saúde. Não foi observada diferença significativa entre as concentrações letais determinadas entre o 3º e 4º estágios para os diferentes extratos, entretanto, as LC₉₀ e LC₉₅ demonstram a maior letalidade do extrato obtido de frutos maduros. Estes resultados sugerem que tanto os extratos de frutos maduros como de folhas secas de M. azedarach apresentam potencial para o desenvolvimento de larvicidas para o Ae. albopictus. inserted in small depressions, secretory ducts associated to the phloem and idioblasts containing calcium oxalate are reported as the mainly morpho-anatomical data for Baccharis coridifolia Ethanolic extracts were obtained by fruits and leaves maceration and the solvent was removed in a rotary evaporator. The lethality bioassays (LC₅₀ , LC₉₀ and LC₉₅ ) were carried out according to the recommendations of the World Health Organization. Leaves and ripe fruits extracts were tested at doses ranging from 0.015 g% to 1,20 g% and 0,015 g% to 0,9 g% ,respectively, for the third and fourth instar larvae. Both extracts showed bioactivity in all doses and instar larvae tested, although ripe fruits extracts showed higher activity. Results indicate that these botanical extracts could be used as an alternative resource to control Ae. albopictus larvae. Santo, Ana P. E. Martins, Ida S. S. Tomy, Sandra C. Ferro, Vicente O. http://sedici.unlp.edu.ar:80/handle/10915/7540 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Comunicacion Anticoagulant in vitro effect of hidrotethanolic extract of edible leaves and flowers of Tropaeolum majus L. (Tropaeolaceae) on human plasma Latin American Journal of Pharmacy; vol. 26, no. 5 Este trabalho teve por objetivo avaliar o efeito anticoagulante in vitro dos extratos de flores e de folhas comestíveis da capuchinha, Tropaeolum majus L. (Tropaeolaceae), através de testes de tempo de trombina (TT), buscando identificar o grupo de compostos responsáveis pela atividade anticoagulante. A análise de variância e o teste Tukey-Kramer revelaram diferença significativa (p < 0,001) entre os grupos teste e controle. Foram dosados os teores de compostos fenólicos e flavonóides dos extratos e das frações bioativas. Os resultados sugerem que estes compostos podem estar relacionados ao efeito anticoagulante dos extratos de capuchinha sobre plasma humano.; The aim of this work was to evaluate the in vitro anticoagulant effect of the benzylisothiocianate, of the extracts of flowers and leaves of nasturtium, Tropaeolum majus L. (Tropaeolaceae), besides the fractions of the leaves extract, through tests of thrombin time (TT) on human plasma. ANOVA and Tukey's test revealed significant (p < 0.001) difference among test and control groups. Determinations of flavonoids and total phenolic contents were carried out with extracts and fractions with anti-thrombin effect. The results suggest that flavonoids can be related to the anticoagulant effect of the nasturtium extracts on human plasma.

2007-01-01T00:00:00Z Este trabalho teve por objetivo avaliar o efeito anticoagulante in vitro dos extratos de flores e de folhas comestíveis da capuchinha, Tropaeolum majus L. (Tropaeolaceae), através de testes de tempo de trombina (TT), buscando identificar o grupo de compostos responsáveis pela atividade anticoagulante. A análise de variância e o teste Tukey-Kramer revelaram diferença significativa (p < 0,001) entre os grupos teste e controle. Foram dosados os teores de compostos fenólicos e flavonóides dos extratos e das frações bioativas. Os resultados sugerem que estes compostos podem estar relacionados ao efeito anticoagulante dos extratos de capuchinha sobre plasma humano. The aim of this work was to evaluate the in vitro anticoagulant effect of the benzylisothiocianate, of the extracts of flowers and leaves of nasturtium, Tropaeolum majus L. (Tropaeolaceae), besides the fractions of the leaves extract, through tests of thrombin time (TT) on human plasma. ANOVA and Tukey's test revealed significant (p < 0.001) difference among test and control groups. Determinations of flavonoids and total phenolic contents were carried out with extracts and fractions with anti-thrombin effect. The results suggest that flavonoids can be related to the anticoagulant effect of the nasturtium extracts on human plasma. Manfron Budel Jane Duarte, Márcia do Rocio http://sedici.unlp.edu.ar:80/handle/10915/7539 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Articulo Morpho-anatomical characters of aerial vegetative organs of Baccharis coridifolia DC. (Asteraceae-Astereae) Latin American Journal of Pharmacy; vol. 26, no. 5 Baccharis coridifolia, representante da família Asteraceae é conhecida como mio-mio, vassourinha, alecrim-do-mato, romerillo, ajenjo del campo, ñio-ñio e toxic groundsel. Apresenta atividades antioxidante, citotóxica e antiinflamatória. Possui tricotecenos macrocíclicos, compostos químicos com importância no tratamento do câncer. Contudo, na América do Sul, é considerada uma planta tóxica para animais devido à presença desse grupo químico. Objetivou-se estudar a morfologia externa e a anatomia das partes vegetativas aéreas de B. coridifolia, com a finalidade de obter dados referentes à identificação e à diferenciação dessa espécie das demais Baccharis, além de fornecer dados taxonômicos à seção Paniculatae. O material botânico foi submetido à s microtécnicas fotônicas e eletrônicas de varredura usuais. Estô- mato anomocítico, mesofilo isobilateral, tricoma tector pluricelular e tricoma glandular, inseridos em pequena depressão, dutos secretores localizados próximos ao floema e presença de cristais de oxalato de cálcio na região perimedular do caule foram as principais características observadas em B. coridifolia; Baccharis coridifolia, a member of the family Asteraceae is known by the common names mio-mio, vassourinha, alecrim-do-mato, romerillo, ajenjo del campo, ñio-ñio and toxic groundsel. It has been demonstrated for this species anti-oxidant, cytotoxic and anti-inflammatory activities. It presentes macrocyclic trichotecenes which are considered relevant for cancer treatment. However, in South America, B. coridifolia is considered as toxic plant for animals, due to these chemical substances. This work has aimed to study the morphology and anatomy of the aerial vegetative organs of B. coridifolia, in order to obtain information concerned to the identification and differentiation from this species to other Baccharis, as well as to supply taxonomic data to the section Paniculatae. The botanical material was prepared according to standard light and scanning microtechniques. Anomocytic stomata, isobilateral mesophyll, multicellular non-glandular and glandular trichomes inserted in small depressions, secretory ducts associated to the phloem and idioblasts containing calcium oxalate are reported as the mainly morpho-anatomical data for Baccharis coridifolia

2007-01-01T00:00:00Z Baccharis coridifolia, representante da família Asteraceae é conhecida como mio-mio, vassourinha, alecrim-do-mato, romerillo, ajenjo del campo, ñio-ñio e toxic groundsel. Apresenta atividades antioxidante, citotóxica e antiinflamatória. Possui tricotecenos macrocíclicos, compostos químicos com importância no tratamento do câncer. Contudo, na América do Sul, é considerada uma planta tóxica para animais devido à presença desse grupo químico. Objetivou-se estudar a morfologia externa e a anatomia das partes vegetativas aéreas de B. coridifolia, com a finalidade de obter dados referentes à identificação e à diferenciação dessa espécie das demais Baccharis, além de fornecer dados taxonômicos à seção Paniculatae. O material botânico foi submetido à s microtécnicas fotônicas e eletrônicas de varredura usuais. Estô- mato anomocítico, mesofilo isobilateral, tricoma tector pluricelular e tricoma glandular, inseridos em pequena depressão, dutos secretores localizados próximos ao floema e presença de cristais de oxalato de cálcio na região perimedular do caule foram as principais características observadas em B. coridifolia Baccharis coridifolia, a member of the family Asteraceae is known by the common names mio-mio, vassourinha, alecrim-do-mato, romerillo, ajenjo del campo, ñio-ñio and toxic groundsel. It has been demonstrated for this species anti-oxidant, cytotoxic and anti-inflammatory activities. It presentes macrocyclic trichotecenes which are considered relevant for cancer treatment. However, in South America, B. coridifolia is considered as toxic plant for animals, due to these chemical substances. This work has aimed to study the morphology and anatomy of the aerial vegetative organs of B. coridifolia, in order to obtain information concerned to the identification and differentiation from this species to other Baccharis, as well as to supply taxonomic data to the section Paniculatae. The botanical material was prepared according to standard light and scanning microtechniques. Anomocytic stomata, isobilateral mesophyll, multicellular non-glandular and glandular trichomes inserted in small depressions, secretory ducts associated to the phloem and idioblasts containing calcium oxalate are reported as the mainly morpho-anatomical data for Baccharis coridifolia Chorilli, Marlus Rimério, Thereana Cristina Oliveira, Anselmo Gomes de Scarpa, María Virginia http://sedici.unlp.edu.ar:80/handle/10915/7538 2014-08-29T15:47:12Z 2007-01-01T00:00:00Z

Articulo Obtaining and characterization of small unilamellar liposomes containing caffeine Latin American Journal of Pharmacy; vol. 26, no. 5 Devido à grande dificuldade de penetração de substâncias ativas através da pele, diferentes sistemas organizados, como os lipossomas, têm sido estudos com o objetivo de aumentar a penetração percutânea. O objetivo deste trabalho foi obter e caracterizar lipossomas unilamelares pequenos contendo cafeína (CAF). Lipossomas compostos por fosfatidilcolina de soja - PC (40 mM) ou PC hidrogenada - PCH (40 mM), acrescidos ou não de colesterol - CHO (6 mM) e CAF (30 mg/mL), foram caracterizados por distribuição de tamanho, determinação de diâmetro médio e eficiência de encapsulação. Observou-se distribuição de tamanho uniforme com baixa polidispersidade. Os diâmetros médios obtidos foram: PC/CHO (64 nm), PC (80 nm), PCH/CHO (85 nm), PC/CAF (145 nm), PC/CHO/CAF (147 nm), PCH/CHO/CAF (152 nm), PCH (166 nm) e PCH/CAF (481 nm). Obteve-se eficiência de encapsulação de 10,84% para PCH/CHO/CAF, seguido de PC/CHO/CAF (6,61%), PCH/CAF (3,07%) e PC/CAF (1,57%).; Due to great difficulty of penetration of drugs through skin, different organized systems, such as liposomes, have been studied in order to increase percutaneous penetration. The aims of this work were to obtain and characterize small unilamellar liposomes containing caffeine (CAF). Liposomes composed by soy phosphatidylcholine - PS (40 mM) or hydrogenated PS - PSH (40 mM), with and without cholesterol - CHO (6 mM) and CAF (30 mg/mL), were characterized by size distribution, determination of mean diameter and encapsulation efficiency. Uniform size distribution with low polidispersity was observed. The mean of diameters obtained were: PS/CHO (64 nm), PS (80 nm), PSH/CHO (85 nm), PS/CAF (145 nm), PS/CHO/CAF (147 nm), PSH/CHO/CAF (152 nm), PSH (166 nm) and PSH/CAF (481 nm). The obtained encapsulation efficiency was 10.84% for PSH/CHO/CAF, followed by PS/CHO (6.61%), PSH/CAF (3.07%) and PS/CAF (1.57%).

2007-01-01T00:00:00Z Devido à grande dificuldade de penetração de substâncias ativas através da pele, diferentes sistemas organizados, como os lipossomas, têm sido estudos com o objetivo de aumentar a penetração percutânea. O objetivo deste trabalho foi obter e caracterizar lipossomas unilamelares pequenos contendo cafeína (CAF). Lipossomas compostos por fosfatidilcolina de soja - PC (40 mM) ou PC hidrogenada - PCH (40 mM), acrescidos ou não de colesterol - CHO (6 mM) e CAF (30 mg/mL), foram caracterizados por distribuição de tamanho, determinação de diâmetro médio e eficiência de encapsulação. Observou-se distribuição de tamanho uniforme com baixa polidispersidade. Os diâmetros médios obtidos foram: PC/CHO (64 nm), PC (80 nm), PCH/CHO (85 nm), PC/CAF (145 nm), PC/CHO/CAF (147 nm), PCH/CHO/CAF (152 nm), PCH (166 nm) e PCH/CAF (481 nm). Obteve-se eficiência de encapsulação de 10,84% para PCH/CHO/CAF, seguido de PC/CHO/CAF (6,61%), PCH/CAF (3,07%) e PC/CAF (1,57%). Due to great difficulty of penetration of drugs through skin, different organized systems, such as liposomes, have been studied in order to increase percutaneous penetration. The aims of this work were to obtain and characterize small unilamellar liposomes containing caffeine (CAF). Liposomes composed by soy phosphatidylcholine - PS (40 mM) or hydrogenated PS - PSH (40 mM), with and without cholesterol - CHO (6 mM) and CAF (30 mg/mL), were characterized by size distribution, determination of mean diameter and encapsulation efficiency. Uniform size distribution with low polidispersity was observed. The mean of diameters obtained were: PS/CHO (64 nm), PS (80 nm), PSH/CHO (85 nm), PS/CAF (145 nm), PS/CHO/CAF (147 nm), PSH/CHO/CAF (152 nm), PSH (166 nm) and PSH/CAF (481 nm). The obtained encapsulation efficiency was 10.84% for PSH/CHO/CAF, followed by PS/CHO (6.61%), PSH/CAF (3.07%) and PS/CAF (1.57%). Santos, Marcelo Henrique dos Corrêa, Rodrigo S. Rocha, Miguel D. Nagem, Tanus Jorge Oliveira, Tania Toledo de Lima, Rosángela D. Oliveira, José R. http://sedici.unlp.edu.ar:80/handle/10915/7537 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Articulo Effect of the chemical constituents isolated from the fruit of Rheedia gardneriana on juveniles hatch of Meloidogyne incognita race 3 Latin American Journal of Pharmacy; vol. 26, no. 5 Os constituintes químicos isolados do fruto (pericarpo) de Rheedia gardneriana ("bacupari"), foram identificados como 7-epiclusianona (I), o ácido oleanólico (II) e uma mistura de sesquiterpenos (III) e testados, in vitro, quanto à sua atividade biológica sobre a eclosão de juvenis do nematóide Meloidogyne incognita raça 3 na concentração de 100 μg.mL^-1 Os resultados mostraram que o ácido oleanólico foi o . mais eficiente, com uma taxa de inibição de eclosão de juvenis de 40,46%, caracterizada como um efeito nematostático, uma vez que a eclosão de juvenis ocorreu quando os ovos tratados pela substância foram transferidos para a água.; The chemical constituents isolated from the fruit (pericarp) of Rheedia gardneriana (bacupari) and identified as 7-epiclusianone (I), oleanolic acid (II), and a mixture of sesquiterpenes (III) were tested to biological activity “in vitro” on juveniles hatch of Meloidogyne incognita race 3 shown nematode effect in 100 μg.mL–1 concentration. The results evidenced that oleanolic acid was most efficient because it showed highest inhibition levels of the juveniles hatch (40.46%), identified as nematostatic effect by the fact of the juveniles hatch had been occurred after translating the eggs for water.

2007-01-01T00:00:00Z Os constituintes químicos isolados do fruto (pericarpo) de Rheedia gardneriana ("bacupari"), foram identificados como 7-epiclusianona (I), o ácido oleanólico (II) e uma mistura de sesquiterpenos (III) e testados, in vitro, quanto à sua atividade biológica sobre a eclosão de juvenis do nematóide Meloidogyne incognita raça 3 na concentração de 100 μg.mL^-1 Os resultados mostraram que o ácido oleanólico foi o . mais eficiente, com uma taxa de inibição de eclosão de juvenis de 40,46%, caracterizada como um efeito nematostático, uma vez que a eclosão de juvenis ocorreu quando os ovos tratados pela substância foram transferidos para a água. The chemical constituents isolated from the fruit (pericarp) of Rheedia gardneriana (bacupari) and identified as 7-epiclusianone (I), oleanolic acid (II), and a mixture of sesquiterpenes (III) were tested to biological activity “in vitro” on juveniles hatch of Meloidogyne incognita race 3 shown nematode effect in 100 μg.mL–1 concentration. The results evidenced that oleanolic acid was most efficient because it showed highest inhibition levels of the juveniles hatch (40.46%), identified as nematostatic effect by the fact of the juveniles hatch had been occurred after translating the eggs for water. Braz, Renato Hechenleitner, Ana A. W. Albuquerque Cavalcanti, Osvaldo http://sedici.unlp.edu.ar:80/handle/10915/7536 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Articulo Extraction, structural modification and characterization of lotus roots polysaccharides (Nelumbo nucifera Gaertn) Latin American Journal of Pharmacy; vol. 26, no. 5 Debido a su biodegradabilidad, recientemente los polisacáridos han sido bastante estudiados para su utilización en sistemas de liberación controlada de farmacos, ya que ellos pueden ser degradados específicamente por la flora bacteriana intestinal. Sin embargo, como regla general los polisacáridos son muy solubles en agua causando una rápida desintegración del revestimiento y liberación precoz del medicamento. Entretanto, estos polí- meros pueden ser fácilmente modificados por el proceso químico de entrecruzamiento (reticulación). En este estudio, polisacáridos de raíz de lotus (Nelumbo nucifera) fueron reticulados con trimetafosfato de sodio (TMPS) por una reacción de fosfatación involucrando los grupos hidrofílicos (hidroxilos) de la molécula, disminuyendo de esta forma su solubilidad en agua y por lo tanto atrasando la liberación del medicamento para las porciones dístales del intestino grueso y delgado. La reacción de reticulación fue confirmada con base en espectros de FTIR, donde la banda de hidroxilos mostró alteración después de la modificación química y también surgió una banda característica de enlace P-O-C. Los análisis térmicos ralizados también son descritos: Análisis termogravimétrico (TGA) y Calorimetría Diferencial de Barrido (DSC).; Due to its biodegradability, lastly polysaccharides have been largely studied for utilization in controlled drug release systems, since they can be specifically degraded by the intestinal bacterial flora. However, as a general rule polysaccharides are very hydrosoluble, causing a fast coating disintegration and precocious drug liberation. Nevertheless, these polysaccharides can be easily modified by chemical processes, like the cross-linking reaction. In this study, lotus (Nelumbo nucifera) root polysaccharides were cross-linked with trisodium trimetaphosphate (TSMP) through a phosphatation reaction involving the hydrophilic groups (hydroxyls) of the molecule, thus decreasing its hydrosolubility and, therefore, putting back the drug release until distal portions of the small and large bowel. Cross-linking reaction confirmation was based in Fourier Transformed Infrared Spectroscopy (FT-IR) analyses, where hydroxyls band showed alteration after chemical modification and a characteristic band of the P-O-C linkage emerged. Thermal analysis (Thermogravimetric Analysis, TGA and Differential Scanning Calorimetry, DSC) were also performed

2007-01-01T00:00:00Z Debido a su biodegradabilidad, recientemente los polisacáridos han sido bastante estudiados para su utilización en sistemas de liberación controlada de farmacos, ya que ellos pueden ser degradados específicamente por la flora bacteriana intestinal. Sin embargo, como regla general los polisacáridos son muy solubles en agua causando una rápida desintegración del revestimiento y liberación precoz del medicamento. Entretanto, estos polí- meros pueden ser fácilmente modificados por el proceso químico de entrecruzamiento (reticulación). En este estudio, polisacáridos de raíz de lotus (Nelumbo nucifera) fueron reticulados con trimetafosfato de sodio (TMPS) por una reacción de fosfatación involucrando los grupos hidrofílicos (hidroxilos) de la molécula, disminuyendo de esta forma su solubilidad en agua y por lo tanto atrasando la liberación del medicamento para las porciones dístales del intestino grueso y delgado. La reacción de reticulación fue confirmada con base en espectros de FTIR, donde la banda de hidroxilos mostró alteración después de la modificación química y también surgió una banda característica de enlace P-O-C. Los análisis térmicos ralizados también son descritos: Análisis termogravimétrico (TGA) y Calorimetría Diferencial de Barrido (DSC). Due to its biodegradability, lastly polysaccharides have been largely studied for utilization in controlled drug release systems, since they can be specifically degraded by the intestinal bacterial flora. However, as a general rule polysaccharides are very hydrosoluble, causing a fast coating disintegration and precocious drug liberation. Nevertheless, these polysaccharides can be easily modified by chemical processes, like the cross-linking reaction. In this study, lotus (Nelumbo nucifera) root polysaccharides were cross-linked with trisodium trimetaphosphate (TSMP) through a phosphatation reaction involving the hydrophilic groups (hydroxyls) of the molecule, thus decreasing its hydrosolubility and, therefore, putting back the drug release until distal portions of the small and large bowel. Cross-linking reaction confirmation was based in Fourier Transformed Infrared Spectroscopy (FT-IR) analyses, where hydroxyls band showed alteration after chemical modification and a characteristic band of the P-O-C linkage emerged. Thermal analysis (Thermogravimetric Analysis, TGA and Differential Scanning Calorimetry, DSC) were also performed Kanis, Luiz A. Generoso, Mariana Soldi, Valdir http://sedici.unlp.edu.ar:80/handle/10915/7535 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Articulo Poly(ethylene-co-methylacrylate)/poly(caprolactone-tryol) films: characterization and mechanical properties Latin American Journal of Pharmacy; vol. 26, no. 5 Neste trabalho foram estudadas as propriedades térmicas, morfológicas e mecânicas de filmes de poli (etileno-co-metil acrilato) (EMA)/poli (caprolactona triol) (PCL-T). Os termogramas de DSC demonstraram que a transição vítrea e fusão do EMA não sofreram alteração com a adição de PCL-T. Através da espectroscopia de infravermelho não foram observadas interações entre as hidroxilas da PCL-T e a carbonila da unidade acrilato do EMA. As microscopias eletrônicas de varredura demonstraram a presença de domínios de PCL-T nas matrizes de EMA, indicativo de imiscibilidade entre os componentes, fato que não alterou significativamente o módulo de elasticidade do EMA, entretanto, com a adição de 40% de PCL-T foi observado uma redução na tensão máxima de ruptura.; Poly(ethylene-co-methyl acrylate) (EMA)/Poly(caprolactone-tryol) (PCL-T) films were studied in terms of thermal, morphological and mechanical properties. The DSC thermograms showed, when PCL-T was added, that there weren´t any changes in the melting point and glass transition of EMA. The infrared spectroscopy showed no interaction between hydroxyl group of PCL-T and carbonyl group of EMA. The scanning electron micrographs showed PCL-T domain in the EMA matrix, suggesting immiscibility of blend. No signifi- cant differences in elasticity modulus were observed with PCL-T addition to the films, although, with addition of 40% PCL-T content, the film tensile strength decreased.

2007-01-01T00:00:00Z Neste trabalho foram estudadas as propriedades térmicas, morfológicas e mecânicas de filmes de poli (etileno-co-metil acrilato) (EMA)/poli (caprolactona triol) (PCL-T). Os termogramas de DSC demonstraram que a transição vítrea e fusão do EMA não sofreram alteração com a adição de PCL-T. Através da espectroscopia de infravermelho não foram observadas interações entre as hidroxilas da PCL-T e a carbonila da unidade acrilato do EMA. As microscopias eletrônicas de varredura demonstraram a presença de domínios de PCL-T nas matrizes de EMA, indicativo de imiscibilidade entre os componentes, fato que não alterou significativamente o módulo de elasticidade do EMA, entretanto, com a adição de 40% de PCL-T foi observado uma redução na tensão máxima de ruptura. Poly(ethylene-co-methyl acrylate) (EMA)/Poly(caprolactone-tryol) (PCL-T) films were studied in terms of thermal, morphological and mechanical properties. The DSC thermograms showed, when PCL-T was added, that there weren´t any changes in the melting point and glass transition of EMA. The infrared spectroscopy showed no interaction between hydroxyl group of PCL-T and carbonyl group of EMA. The scanning electron micrographs showed PCL-T domain in the EMA matrix, suggesting immiscibility of blend. No signifi- cant differences in elasticity modulus were observed with PCL-T addition to the films, although, with addition of 40% PCL-T content, the film tensile strength decreased. Hick, Adriana S. Paczkowski, Matías G. Gadano, Andrea B. Carballo, Marta A. http://sedici.unlp.edu.ar:80/handle/10915/7534 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Articulo Genotoxicity biomarkers in arsenic exposed population Latin American Journal of Pharmacy; vol. 26, no. 5 El objetivo del presente trabajo es analizar el impacto subclínico sobre la salud que resulta de la exposición al arsénico mediante el agua de bebida, en dos grupos de individuos, uno de la provincia de Santiago del Estero (La Firmeza, Santos Lugares y Urutaú, con un n = 20) y el otro de la provincia de San- ta Fe (Providencia, con un n = 20), como estudio piloto. La evaluación se llevó a cabo mediante el análisis de biomarcadores de efecto que actúan como dosímetros biológicos. Se realizaron determinaciones de índi- ce mitótico (IM), cinética de proliferación celular (CPC), micronúcleo en mucosa oral (MnMO), frecuencia de intercambio de cromátides hermanas (ICH), y electroforesis de una sola célula (Test del cometa). Los resultados obtenidos muestran una disminución estadísticamente significativa en el IM (p < 0,003) y un in- cremento en la frecuencia de Mn (p < 0,0001) para el grupo de Santiago del Estero, mientras que en el grupo de la provincia de Santa Fe, los valores no difieren de los que presenta el grupo control. Los hallazgos sugieren que la exposición al arsénico en forma accidental no resulta inocua frente a una evaluación toxicogenética, siendo la susceptibilidad individual uno de los factores de mayor incidencia.; The aim of this work is to evaluate the subclinical impact of arsenic in drinking water over the population health in two individual groups, one of them of Santiago del Estero province (La Firmeza, Santos Lugares y Urutaú, n = 20), and the other one of Santa Fe province (Providencia, n = 20), as a pilot study. The evaluation was developed through effect biomarkers such as Mitotic Index (MI), Cellular Proliferation Kinetics (CPK), Micronucleous in epithelial cells (Mn), Sister Chro- matid Exchanges (SCE) and Single Cell Electrophoresis (Comet assay). Our results shows a significative de- crease in MI (p < 0.003) and significative increase (p < 0.00001) in Mn frequency in Santiago del Estero, while in Santa Fe, differences between control and exposed group, were not observed. These findings suggest that ar- senic exposure is dangerous against this toxicogenetic evaluation and individual susceptibility is one of the most important incidence factors.

2007-01-01T00:00:00Z El objetivo del presente trabajo es analizar el impacto subclínico sobre la salud que resulta de la exposición al arsénico mediante el agua de bebida, en dos grupos de individuos, uno de la provincia de Santiago del Estero (La Firmeza, Santos Lugares y Urutaú, con un n = 20) y el otro de la provincia de San- ta Fe (Providencia, con un n = 20), como estudio piloto. La evaluación se llevó a cabo mediante el análisis de biomarcadores de efecto que actúan como dosímetros biológicos. Se realizaron determinaciones de índi- ce mitótico (IM), cinética de proliferación celular (CPC), micronúcleo en mucosa oral (MnMO), frecuencia de intercambio de cromátides hermanas (ICH), y electroforesis de una sola célula (Test del cometa). Los resultados obtenidos muestran una disminución estadísticamente significativa en el IM (p < 0,003) y un in- cremento en la frecuencia de Mn (p < 0,0001) para el grupo de Santiago del Estero, mientras que en el grupo de la provincia de Santa Fe, los valores no difieren de los que presenta el grupo control. Los hallazgos sugieren que la exposición al arsénico en forma accidental no resulta inocua frente a una evaluación toxicogenética, siendo la susceptibilidad individual uno de los factores de mayor incidencia. The aim of this work is to evaluate the subclinical impact of arsenic in drinking water over the population health in two individual groups, one of them of Santiago del Estero province (La Firmeza, Santos Lugares y Urutaú, n = 20), and the other one of Santa Fe province (Providencia, n = 20), as a pilot study. The evaluation was developed through effect biomarkers such as Mitotic Index (MI), Cellular Proliferation Kinetics (CPK), Micronucleous in epithelial cells (Mn), Sister Chro- matid Exchanges (SCE) and Single Cell Electrophoresis (Comet assay). Our results shows a significative de- crease in MI (p < 0.003) and significative increase (p < 0.00001) in Mn frequency in Santiago del Estero, while in Santa Fe, differences between control and exposed group, were not observed. These findings suggest that ar- senic exposure is dangerous against this toxicogenetic evaluation and individual susceptibility is one of the most important incidence factors. Souza, Claudia R. F. Bott, Rubiana F. Oliveira, Wanderley P. http://sedici.unlp.edu.ar:80/handle/10915/7533 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Articulo Optimization of the extraction of flavonoids compounds from herbal material using experimental design and multi-response analysis Latin American Journal of Pharmacy; vol. 26, no. 5 El análisis estadístico, así como el diseño experimental, el análisis de regresión y el análisis multi-respuesta son potentes herramientas para la caracterización y la optimización de los procesos farmacéuticos, permitindo el estudio de los efectos de variables que afectan el proceso y sus posibles interacciones. En este trabajo, los métodos estadísticos fueron utilizados en el desarrollo y la optimizacion del proceso de extracción de los compuestos flavonoides (marcadores activos) de hojas de Bauhinia forficata Link por soluciones etanol:agua. Los resultados permitieron la determinación de las variables que afectan el proceso de extracción y de las mejores condiciones para la extracción de compuestos activos de las hojas de Bauhinia forficata Link.; Statistical analysis such as experimental design, regression and multi-response analyses are powerful tools for the characterization and optimization of the pharmaceutical process by studying the effects of variables affecting them and their possible interactions. In this paper, these analyses were detailed presented during the development and optimization of a process for the extraction of flavonoid compounds (active markers) from Bauhinia forficata Link leaves by hydro-ethanolic solutions. The results permitted the determination of the variables affecting the extraction process and allowed the determination of the best conditions for the extraction of active compounds from the leaves of Bauhinia forficata Link.

2007-01-01T00:00:00Z El análisis estadístico, así como el diseño experimental, el análisis de regresión y el análisis multi-respuesta son potentes herramientas para la caracterización y la optimización de los procesos farmacéuticos, permitindo el estudio de los efectos de variables que afectan el proceso y sus posibles interacciones. En este trabajo, los métodos estadísticos fueron utilizados en el desarrollo y la optimizacion del proceso de extracción de los compuestos flavonoides (marcadores activos) de hojas de Bauhinia forficata Link por soluciones etanol:agua. Los resultados permitieron la determinación de las variables que afectan el proceso de extracción y de las mejores condiciones para la extracción de compuestos activos de las hojas de Bauhinia forficata Link. Statistical analysis such as experimental design, regression and multi-response analyses are powerful tools for the characterization and optimization of the pharmaceutical process by studying the effects of variables affecting them and their possible interactions. In this paper, these analyses were detailed presented during the development and optimization of a process for the extraction of flavonoid compounds (active markers) from Bauhinia forficata Link leaves by hydro-ethanolic solutions. The results permitted the determination of the variables affecting the extraction process and allowed the determination of the best conditions for the extraction of active compounds from the leaves of Bauhinia forficata Link. Uribarri Hernández, Evangelina González Lago, Mayra de la C. Martínez Álvarez, Luis Marrero Delange, David Medina, Miriam Cora Méndez Antolín, Ernesto J. Laguna Granja, Abilio González Canavaciolo, Víctor L. http://sedici.unlp.edu.ar:80/handle/10915/7532 2016-03-30T18:57:12Z 2007-01-01T00:00:00Z

Articulo Physico-mechanical characterization and interactions with excipients of D-003, a mixture of fatty acids isolated from sugar cane wax Latin American Journal of Pharmacy; vol. 26, no. 5 El D-003 es un nuevo producto que consiste en una mezcla de ácidos grasos de elevado peso molecular (desde C24:0 hasta C36:0), donde cada uno de ellos está en una proporción reproducible. Este producto presenta efectos antiplaquetarios y como reductor del colesterol. Como parte de los estudios de formulación del D-003 fueron llevadas a cabo la caracterización físico-mecánica y su interacción con excipientes para tabletas. El presente estudio utilizó la calorimetría diferencial de barrido (CDB) y la termogravimetría (TG) con el objetivo de evaluar la estabilidad térmica y las transiciones características del D-003 en presencia de diversos excipientes. Fueron medidas otras propiedades, tales como el ángulo de contacto, solubilidad, humectabilidad, distribución del tamaño de partículas, propiedades de fluido, densidades por asentamiento, por vertido y de alta presión, así como la reducción de volumen bajo compresión. Estos estudios, según nuestro conocimiento, no han sido previamente reportados para estos ácidos. Los estudios por CDB demostraron que no hubo interacción química o física entre el D-003 y los excipientes investigados. También, los análisis termogravimétricos probaron la elevada estabilidad térmica del D-003, el cual es estable y funde sin descomposición hasta temperaturas tan elevadas como 220 °C. Los lotes industriales estudiados mostraron adecuada pureza y distribución uniforme del tamaño de partícula, buenas propiedades de fluido, compresibilidad y cohesión, lo cual sustenta que sea un polvo apropiado para la elaboración de tabletas revestidas como forma terminada. Sin embargo, la muy baja solubilidad del D-003 en la mayoría de los disolventes, especialmente en agua, es el aspecto más crítico para el desarrollo la forma terminada, lo cual es importante para decidir la formulación del revestimiento.; D-003 is a new product consisting of a mixture of fatty acids of very high molecular weight (from C24:0 to C36:0) in a reproducible proportion each, with cholesterol-lowering, and antiplatelet effects. As part of the formulation studies of D-003 a physico-mechanical characterization and its interaction with tablet excipients were carried out. The present study used differential scanning calorimetry (DSC) and thermogravimetry (TG) in order to evaluate the characteristic transitions and thermal stability of D- 003 in presence of several excipients. Other properties, such as the angle of contact, solubility, wettability, particle size distribution, flow properties, tapped, bulk and at high pressure densities, as well as volume reduction under compression were measured. These studies, to our knowledge, have not been previously reported to these fatty acids. DSC studies demonstrated that there was no chemical or physical interaction between D-003 and the investigated excipients. Also, thermogravimetric analysis proved the high thermal stability of D-003, which melts without decomposition and is stable at temperatures as high as 220 °C. Industrial batches of D-003 showed adequate purity and uniform particle size distribution, good flow properties, compressibility and compactability, which support a suitable powder for manufacturing film-coated tablets as finished forms. Nevertheless, the very low solubility of D-003 in most solvents, especially in water, is the most critical aspect for developing the finished form, being relevant to decide the coating formulation.

2007-01-01T00:00:00Z El D-003 es un nuevo producto que consiste en una mezcla de ácidos grasos de elevado peso molecular (desde C24:0 hasta C36:0), donde cada uno de ellos está en una proporción reproducible. Este producto presenta efectos antiplaquetarios y como reductor del colesterol. Como parte de los estudios de formulación del D-003 fueron llevadas a cabo la caracterización físico-mecánica y su interacción con excipientes para tabletas. El presente estudio utilizó la calorimetría diferencial de barrido (CDB) y la termogravimetría (TG) con el objetivo de evaluar la estabilidad térmica y las transiciones características del D-003 en presencia de diversos excipientes. Fueron medidas otras propiedades, tales como el ángulo de contacto, solubilidad, humectabilidad, distribución del tamaño de partículas, propiedades de fluido, densidades por asentamiento, por vertido y de alta presión, así como la reducción de volumen bajo compresión. Estos estudios, según nuestro conocimiento, no han sido previamente reportados para estos ácidos. Los estudios por CDB demostraron que no hubo interacción química o física entre el D-003 y los excipientes investigados. También, los análisis termogravimétricos probaron la elevada estabilidad térmica del D-003, el cual es estable y funde sin descomposición hasta temperaturas tan elevadas como 220 °C. Los lotes industriales estudiados mostraron adecuada pureza y distribución uniforme del tamaño de partícula, buenas propiedades de fluido, compresibilidad y cohesión, lo cual sustenta que sea un polvo apropiado para la elaboración de tabletas revestidas como forma terminada. Sin embargo, la muy baja solubilidad del D-003 en la mayoría de los disolventes, especialmente en agua, es el aspecto más crítico para el desarrollo la forma terminada, lo cual es importante para decidir la formulación del revestimiento. D-003 is a new product consisting of a mixture of fatty acids of very high molecular weight (from C24:0 to C36:0) in a reproducible proportion each, with cholesterol-lowering, and antiplatelet effects. As part of the formulation studies of D-003 a physico-mechanical characterization and its interaction with tablet excipients were carried out. The present study used differential scanning calorimetry (DSC) and thermogravimetry (TG) in order to evaluate the characteristic transitions and thermal stability of D- 003 in presence of several excipients. Other properties, such as the angle of contact, solubility, wettability, particle size distribution, flow properties, tapped, bulk and at high pressure densities, as well as volume reduction under compression were measured. These studies, to our knowledge, have not been previously reported to these fatty acids. DSC studies demonstrated that there was no chemical or physical interaction between D-003 and the investigated excipients. Also, thermogravimetric analysis proved the high thermal stability of D-003, which melts without decomposition and is stable at temperatures as high as 220 °C. Industrial batches of D-003 showed adequate purity and uniform particle size distribution, good flow properties, compressibility and compactability, which support a suitable powder for manufacturing film-coated tablets as finished forms. Nevertheless, the very low solubility of D-003 in most solvents, especially in water, is the most critical aspect for developing the finished form, being relevant to decide the coating formulation. Andréo Filho, Newton Pessole, Letícia Yang, Ming Issa, Michele Ferraz, Humberto http://sedici.unlp.edu.ar:80/handle/10915/7531 2013-10-17T12:02:29Z 2007-01-01T00:00:00Z

Articulo Application of gastro-resistant coating on didanosine tablets using differents machines: conventional or perforated pan coater and fluid bed Latin American Journal of Pharmacy; vol. 26, no. 5 Los comprimidos gastro-resistentes de didanosina fueron desarrollados utilizando diferentes equipos de recubrimiento. Cuatro formulaciones de núcleos fueron preparadas y evaluadas por medio del perfil de disolución y posteriormente la formulación seleccionada fue recubierta (3,5; 5,5 y 8,0 %, p/p) en bombo grageador convencional, tambor perforado y lecho fluido, siendo evaluada la gastro-resistencia. Todos los equipos y niveles de revestimiento resultaron en comprimidos gastro-resistentes; sin embargo, para el de mayor nivel de revestimiento no fue observada la permeación del líquido de disolución en los núcleos. No hubo diferencia en el revestimiento frente a los diferentes equipos utilizados, siendo las variaciones estructurales, funcionales y operacionales las razones para la preferencia del uso de cualquiera de ellos.; Formulation of didanosine gastro-resistant tablets was devel- oped using different coating machines. Four core formulations were prepared and tested about drug dissolution. The selected formulation was coating (3.5, 5.5 and 8.0% w/w) in conventional and perforated coating machines and fluid bed equipment and assay about gastro-resistant properties. All coating equipments and levels of coating provided gastro-resistant tablets, however only for the bigger level of coating was not observed permeation of dissolution mean into the tablets. No difference was noted between the efficacy of coating equipments, therefore structural, functional and operational variations are the cause to preference one to another.

2007-01-01T00:00:00Z Los comprimidos gastro-resistentes de didanosina fueron desarrollados utilizando diferentes equipos de recubrimiento. Cuatro formulaciones de núcleos fueron preparadas y evaluadas por medio del perfil de disolución y posteriormente la formulación seleccionada fue recubierta (3,5; 5,5 y 8,0 %, p/p) en bombo grageador convencional, tambor perforado y lecho fluido, siendo evaluada la gastro-resistencia. Todos los equipos y niveles de revestimiento resultaron en comprimidos gastro-resistentes; sin embargo, para el de mayor nivel de revestimiento no fue observada la permeación del líquido de disolución en los núcleos. No hubo diferencia en el revestimiento frente a los diferentes equipos utilizados, siendo las variaciones estructurales, funcionales y operacionales las razones para la preferencia del uso de cualquiera de ellos. Formulation of didanosine gastro-resistant tablets was devel- oped using different coating machines. Four core formulations were prepared and tested about drug dissolution. The selected formulation was coating (3.5, 5.5 and 8.0% w/w) in conventional and perforated coating machines and fluid bed equipment and assay about gastro-resistant properties. All coating equipments and levels of coating provided gastro-resistant tablets, however only for the bigger level of coating was not observed permeation of dissolution mean into the tablets. No difference was noted between the efficacy of coating equipments, therefore structural, functional and operational variations are the cause to preference one to another.