http://sedici.unlp.edu.ar:80/handle/10915/85152 2026-11-15T17:02:30Z 2026-11-15T17:02:30Z Wolfenson, Laura Silbestro, MB Sosa, F. E. Romero, S. R. Muzzio, Marina Anello, Melina http://sedici.unlp.edu.ar:80/handle/10915/198326 2026-09-07T20:27:05Z 2026-01-01T00:00:00Z

Objeto de conferencia XXXVI Jornadas Argentinas de Mastozoología (JAM) (El Calafate, Santa Cruz, 23 al 26 de septiembre de 2025); 36° Jornadas Argentinas de Mastozoología La llama, Lama glama, es una especie autóctona de importancia económica y sociocultural para las poblaciones puneñas del noroeste argentino. Mantener su diversidad genética y fenotípica es fundamental, no sólo para conservar la especie sino también para promover una producción sustentable. La información disponible sobre la diversidad genética de las poblaciones de llamas en Argentina es limitada. En este estudio, utilizamos genotipado por secuenciación (GBS), un enfoque genómico, para estimar la diversidad genética, evaluar su estado de conservación y niveles de endogamia. Para ello se genotiparon 155 muestras de ADN de 8 sitios de Jujuy y 4 de Catamarca. Las secuencias se analizaron siguiendo el flujo de trabajo TASSEL5.0v2 y usando el genoma de Camelus ferus (BCGSAC_Cfer_1.0) como referencia. Para estudiar la diversidad y la estructura genética se empleó R y los softwares PLINK y ADMIXTURE. Luego de los filtrados de calidad, se obtuvieron 11.153 SNPs en 138 muestras, con un promedio de 301 marcadores por cromosoma. Las poblaciones de ambas provincias se diferencian genéticamente entre sí. No hay diferenciación dentro Catamarca pero sí en Jujuy, donde se distinguen al menos 4 grupos. Predominaron segmentos de homocigosis (ROH) cortos, indicando consanguinidad antigua. Sin embargo, en Pozuelos, Jujuy, se evidenciaron eventos recientes de homocigosidad en llamas con defectos congénitos. Investigamos la región cromosómica en busca de posibles genes candidatos asociados a estos rasgos y proponemos los genes MYO15A y USH1G como candidatos vinculados a la sordera asociada a la pigmentación, aunque se requieren estudios adicionales para confirmar esta relación. Este estudio constituye un primer paso para comprender la diversidad genética de las llamas argentinas, y subraya la necesidad de reducir los niveles actuales de endogamia e implementar un monitoreo continuo que permita mejorar las decisiones de manejo y respaldar un sistema de producción sostenible para la especie.

2026-01-01T00:00:00Z La llama, Lama glama, es una especie autóctona de importancia económica y sociocultural para las poblaciones puneñas del noroeste argentino. Mantener su diversidad genética y fenotípica es fundamental, no sólo para conservar la especie sino también para promover una producción sustentable. La información disponible sobre la diversidad genética de las poblaciones de llamas en Argentina es limitada. En este estudio, utilizamos genotipado por secuenciación (GBS), un enfoque genómico, para estimar la diversidad genética, evaluar su estado de conservación y niveles de endogamia. Para ello se genotiparon 155 muestras de ADN de 8 sitios de Jujuy y 4 de Catamarca. Las secuencias se analizaron siguiendo el flujo de trabajo TASSEL5.0v2 y usando el genoma de Camelus ferus (BCGSAC_Cfer_1.0) como referencia. Para estudiar la diversidad y la estructura genética se empleó R y los softwares PLINK y ADMIXTURE. Luego de los filtrados de calidad, se obtuvieron 11.153 SNPs en 138 muestras, con un promedio de 301 marcadores por cromosoma. Las poblaciones de ambas provincias se diferencian genéticamente entre sí. No hay diferenciación dentro Catamarca pero sí en Jujuy, donde se distinguen al menos 4 grupos. Predominaron segmentos de homocigosis (ROH) cortos, indicando consanguinidad antigua. Sin embargo, en Pozuelos, Jujuy, se evidenciaron eventos recientes de homocigosidad en llamas con defectos congénitos. Investigamos la región cromosómica en busca de posibles genes candidatos asociados a estos rasgos y proponemos los genes MYO15A y USH1G como candidatos vinculados a la sordera asociada a la pigmentación, aunque se requieren estudios adicionales para confirmar esta relación. Este estudio constituye un primer paso para comprender la diversidad genética de las llamas argentinas, y subraya la necesidad de reducir los niveles actuales de endogamia e implementar un monitoreo continuo que permita mejorar las decisiones de manejo y respaldar un sistema de producción sostenible para la especie. Parisi, Julieta Marcia Castrigiovanni, Daniel Grau, Jorge Enrique Gregorutti, Ricardo Walter Elsner, Cecilia Inés http://sedici.unlp.edu.ar:80/handle/10915/196816 2026-07-08T20:25:17Z 2021-01-01T00:00:00Z

Articulo Localized corrosion of austenitic stainless steel in cell culture medium and evaluation of the cytotoxicity of the released ions Ciencia y Tecnología de los Materiales; no. 11 El acero inoxidable austenítico ASTM F745 ha sido sometido a corrosión localizada en medio de cultivo celular y solución acuosa de NaCl, cuyas concentraciones de cloruro fueron 4413,2 mg L⁻¹ y 5423,1 mg L⁻¹, respectivamente. Se ha observado que la susceptibilidad a la corrosión localizada fue menor en el medio de cultivo debido a su menor contenido de cloruros. El Fe fue el metal más liberado en ambos medios durante el proceso de corrosión. Las concentraciones fueron 0,570 mg L⁻¹ en la solución acuosa de NaCl y 0,480 mg L⁻¹ en el medio de cultivo. Las concentraciones de Cr y Mo fueron similares en ambos medios, habiendo sido 0,040 mg L⁻¹ y 0,010 mg L⁻¹, respectivamente, mientras que Ni solo se detectó en la solución acuosa de NaCl, 0,100 mg L⁻¹. Estas concentraciones de iones liberados no provocaron efectos citotóxicos en células similares a osteoblastos.; Austenitic stainless steel ASTM F745 has been submitted to localized corrosion in cell culture medium and NaCl aqueous solution, whose chloride concentrations were 4413,2 mg L⁻¹ and 5423,1 mg L⁻¹, respectively. It has been observed that the susceptibility to localized corrosion was lower in the culture medium due to its lower chloride content. Fe was the most released metal in both media during the corrosion process. The concentrations were 0,570 mg L-1 in the NaCl aqueous solution and 0,480 mg L⁻¹ in the culture medium. The concentrations of Cr and Mo released were similar in both media, having been 0,040 mg L⁻¹ and 0,010 mg L⁻¹, respectively, while Ni was only detected in the NaCl aqueous solution, 0,100 mg L⁻¹. These concentrations of ions released did not cause cytotoxic effects in osteoblast-like cells.

2021-01-01T00:00:00Z El acero inoxidable austenítico ASTM F745 ha sido sometido a corrosión localizada en medio de cultivo celular y solución acuosa de NaCl, cuyas concentraciones de cloruro fueron 4413,2 mg L⁻¹ y 5423,1 mg L⁻¹, respectivamente. Se ha observado que la susceptibilidad a la corrosión localizada fue menor en el medio de cultivo debido a su menor contenido de cloruros. El Fe fue el metal más liberado en ambos medios durante el proceso de corrosión. Las concentraciones fueron 0,570 mg L⁻¹ en la solución acuosa de NaCl y 0,480 mg L⁻¹ en el medio de cultivo. Las concentraciones de Cr y Mo fueron similares en ambos medios, habiendo sido 0,040 mg L⁻¹ y 0,010 mg L⁻¹, respectivamente, mientras que Ni solo se detectó en la solución acuosa de NaCl, 0,100 mg L⁻¹. Estas concentraciones de iones liberados no provocaron efectos citotóxicos en células similares a osteoblastos. Austenitic stainless steel ASTM F745 has been submitted to localized corrosion in cell culture medium and NaCl aqueous solution, whose chloride concentrations were 4413,2 mg L⁻¹ and 5423,1 mg L⁻¹, respectively. It has been observed that the susceptibility to localized corrosion was lower in the culture medium due to its lower chloride content. Fe was the most released metal in both media during the corrosion process. The concentrations were 0,570 mg L-1 in the NaCl aqueous solution and 0,480 mg L⁻¹ in the culture medium. The concentrations of Cr and Mo released were similar in both media, having been 0,040 mg L⁻¹ and 0,010 mg L⁻¹, respectively, while Ni was only detected in the NaCl aqueous solution, 0,100 mg L⁻¹. These concentrations of ions released did not cause cytotoxic effects in osteoblast-like cells. Orsi, Federico Monat, Juliana Enrique, Nicolás Jorge Milesi, Verónica Castillo, Karen Raingo, Jesica Martín, Pedro http://sedici.unlp.edu.ar:80/handle/10915/196337 2026-06-29T20:24:24Z 2026-01-01T00:00:00Z

Articulo Pharmacological Research; vol. 229 Large-conductance Ca²⁺- and voltage-activated K⁺ (BK) channels are critical regulators of neuronal excitability and have been implicated in multiple epileptic syndromes. Their functional diversity arises from the co-assembly of pore-forming α-subunits with auxiliary β subunits, among which β4 is highly expressed in distinct regions of the central nervous system, including hippocampal Dentate Gyrus Granule Cells (DGGCs). Here, we identify montelukast (MTK), a clinically approved cysteinyl-leukotriene receptor antagonist, as a direct activator of BK channels, with markedly enhanced efficacy in the presence of the β1 and β4 subunits. MTK acts at submicromolar concentrations and facilitates channel opening by altering the energetics of the pore domain, independent of voltage-sensor activation or Ca²⁺ binding to the cytosolic gating ring. In mouse hippocampal slices, MTK reduces intrinsic excitability of DGGCs by decreasing input resistance and enhancing the afterhyperpolarization, effects fully reversed by the BK channel blocker paxilline. Experiments using physiological DGGCs action potential voltage waveforms confirm that MTK enhances subthreshold and evoked BK currents in α/β4 channels as selective targets. Altogether, our findings suggest that MTK, beyond its known anti-inflammatory properties, may modulate neural excitability through direct BK channel activation, offering a novel therapeutic strategy for seizure suppression.

2026-01-01T00:00:00Z Large-conductance Ca²⁺- and voltage-activated K⁺ (BK) channels are critical regulators of neuronal excitability and have been implicated in multiple epileptic syndromes. Their functional diversity arises from the co-assembly of pore-forming α-subunits with auxiliary β subunits, among which β4 is highly expressed in distinct regions of the central nervous system, including hippocampal Dentate Gyrus Granule Cells (DGGCs). Here, we identify montelukast (MTK), a clinically approved cysteinyl-leukotriene receptor antagonist, as a direct activator of BK channels, with markedly enhanced efficacy in the presence of the β1 and β4 subunits. MTK acts at submicromolar concentrations and facilitates channel opening by altering the energetics of the pore domain, independent of voltage-sensor activation or Ca²⁺ binding to the cytosolic gating ring. In mouse hippocampal slices, MTK reduces intrinsic excitability of DGGCs by decreasing input resistance and enhancing the afterhyperpolarization, effects fully reversed by the BK channel blocker paxilline. Experiments using physiological DGGCs action potential voltage waveforms confirm that MTK enhances subthreshold and evoked BK currents in α/β4 channels as selective targets. Altogether, our findings suggest that MTK, beyond its known anti-inflammatory properties, may modulate neural excitability through direct BK channel activation, offering a novel therapeutic strategy for seizure suppression. Castrogiovanni, Daniel Cayetano http://sedici.unlp.edu.ar:80/handle/10915/196293 2026-06-29T20:24:25Z 2026-04-01T00:00:00Z

Articulo Life Sciences; vol. 390 Receptors for orexigenic hormones are broadly expressed in different tissues. Growth hormone secretagogue receptor (GHSR) is the receptor for the gastric peptide hormone ghrelin, although it further displays an intrinsic ligand-independent activity. The liver-expressed antimicrobial peptide 2 (LEAP2) has been recently identified as GHSR endogenous antagonist and inverse agonist. To understand LEAP2 effects on cardiac performance, we assessed (in-vivo) Wistar (WT) and spontaneously hypertensive rats (SHR), isolated hearts (ex-vivo) and car­ diomyocytes (in-vitro). Intravenous LEAP2 injection reduced arterial pressure, with a greater effect in SHR. LEAP2 also reduced cardiac inotropism in both WT and SHR, whereas a negative chronotropy was observed only in SHR. LEAP2 perfusion reduced intraventricular pressure in isolated SHR hearts and attenuated the responses to isoproterenol on coronary flow, whereas responses to acetylcholine were almost unaffected by LEAP2. Fluorescent LEAP2 or ghrelin labeled cardiomyocytes. LEAP2 or the synthetic inverse agonist PF04628935 produced an equipotent reduction in maximum contraction speed, reduced cardiomyocyte shortening area, and attenuated the responses to isoproterenol, but not to acetylcholine. Plasma levels of ghrelin and LEAP2 were not different between strains, but higher GHSR levels were found in SHR hearts. In conclusion, LEAP2 exerted marked cardiovascular effects, reducing arterial pressure and left ventricular systolic performance in both WT and SHR, with a greater impact in hypertensive animals. However, GHSR involvement in SHR cardiac abnor­ malities is unrelated to the circulating levels of its endogenous ligands and instead seems to depend on altered cardiac GHSR expression and function. The cardiac LEAP2 interaction with β-adrenergic receptors reveals an important cardioprotective potential. La lista completa de autores que integran el documento puede consultarse en el archivo.

2026-04-01T00:00:00Z Receptors for orexigenic hormones are broadly expressed in different tissues. Growth hormone secretagogue receptor (GHSR) is the receptor for the gastric peptide hormone ghrelin, although it further displays an intrinsic ligand-independent activity. The liver-expressed antimicrobial peptide 2 (LEAP2) has been recently identified as GHSR endogenous antagonist and inverse agonist. To understand LEAP2 effects on cardiac performance, we assessed (in-vivo) Wistar (WT) and spontaneously hypertensive rats (SHR), isolated hearts (ex-vivo) and car­ diomyocytes (in-vitro). Intravenous LEAP2 injection reduced arterial pressure, with a greater effect in SHR. LEAP2 also reduced cardiac inotropism in both WT and SHR, whereas a negative chronotropy was observed only in SHR. LEAP2 perfusion reduced intraventricular pressure in isolated SHR hearts and attenuated the responses to isoproterenol on coronary flow, whereas responses to acetylcholine were almost unaffected by LEAP2. Fluorescent LEAP2 or ghrelin labeled cardiomyocytes. LEAP2 or the synthetic inverse agonist PF04628935 produced an equipotent reduction in maximum contraction speed, reduced cardiomyocyte shortening area, and attenuated the responses to isoproterenol, but not to acetylcholine. Plasma levels of ghrelin and LEAP2 were not different between strains, but higher GHSR levels were found in SHR hearts. In conclusion, LEAP2 exerted marked cardiovascular effects, reducing arterial pressure and left ventricular systolic performance in both WT and SHR, with a greater impact in hypertensive animals. However, GHSR involvement in SHR cardiac abnor­ malities is unrelated to the circulating levels of its endogenous ligands and instead seems to depend on altered cardiac GHSR expression and function. The cardiac LEAP2 interaction with β-adrenergic receptors reveals an important cardioprotective potential. Flores, Constanza Yanel Peri Ibáñez, E.S. Achilli, E.E. Pedano, M.L. Castello, A.A. Grasselli, M. http://sedici.unlp.edu.ar:80/handle/10915/196193 2026-06-24T20:27:39Z 2026-01-01T00:00:00Z

Comunicacion Next Bioengineering; vol. 2 Lateral flow assays (LFAs) have evolved significantly, expanding well beyond clinical diagnostics into a versatile, rapid, and cost-effective platform for point-of-care applications. Gold nanoparticles (AuNPs) are the most used nanomaterial for the preparation of labels in LFA devices. However, their monochromaticity limits the advantage of human eyes in detecting many colors simultaneously and, therefore, the potentiality of LFA. To address this limitation, this work reports a facile and robust synthesis of blue dielectric-coated gold core-shell nanospheres engineered for LFA applications. This innovative nanostructure modulates the localized surface plasmon reso­ nance to achieve a distinct color contrast, opening new avenues for the simultaneous co-detection of multiple antigens on a single test strip without compromising the simplicity of visual inspection.

2026-01-01T00:00:00Z Lateral flow assays (LFAs) have evolved significantly, expanding well beyond clinical diagnostics into a versatile, rapid, and cost-effective platform for point-of-care applications. Gold nanoparticles (AuNPs) are the most used nanomaterial for the preparation of labels in LFA devices. However, their monochromaticity limits the advantage of human eyes in detecting many colors simultaneously and, therefore, the potentiality of LFA. To address this limitation, this work reports a facile and robust synthesis of blue dielectric-coated gold core-shell nanospheres engineered for LFA applications. This innovative nanostructure modulates the localized surface plasmon reso­ nance to achieve a distinct color contrast, opening new avenues for the simultaneous co-detection of multiple antigens on a single test strip without compromising the simplicity of visual inspection. Peluffo, R. Daniel Itri, Rosângela Barbosa, Leandro R. S. Alonso, Silvia del Valle González Flecha, F. Luis http://sedici.unlp.edu.ar:80/handle/10915/193792 2026-04-30T20:23:44Z 2025-04-01T00:00:00Z

Articulo Biophysical Reviews; vol. 17, no. 2 This Editorial for Volume 17 Issue 2 of Biophysical Reviews introduces the contents of the second Special Issue on the Latin American Federation of Biophysical Societies (LAFeBS). Biophysical Reviews is the official journal of the International Union for Pure and Applied Biophysics (IUPAB). The multidisciplinary scope of the articles in this issue reflects LAFeBS’s commitment to highlighting regional contributions to the advancement of biophysics across all its branches.

2025-04-01T00:00:00Z This Editorial for Volume 17 Issue 2 of Biophysical Reviews introduces the contents of the second Special Issue on the Latin American Federation of Biophysical Societies (LAFeBS). Biophysical Reviews is the official journal of the International Union for Pure and Applied Biophysics (IUPAB). The multidisciplinary scope of the articles in this issue reflects LAFeBS’s commitment to highlighting regional contributions to the advancement of biophysics across all its branches. Cassano, Daniela Alejandra Barrile, Franco Reynaldo, Mirta Beatriz Fernández, Gimena Cornejo, María Paula Tolosa, María José Anahí Heredia, María F. Ferreira, Nathalia Fideles, Higor J De Francesco, Pablo Nicolás Schiöth, Helgi B. Rorato, Rodrigo Perelló, Mario Carlos http://sedici.unlp.edu.ar:80/handle/10915/193621 2026-04-27T20:23:12Z 2025-08-04T00:00:00Z

Articulo Communications Biology; vol. 8 no. 1 Hunger enhances the consumption of rewarding foods, but the neurobiological basis of this adaptation remains unclear. We hypothesize that agouti-related protein (AgRP) neurons in the hypothalamic arcuate nucleus (ARH) promote the consumption of rewarding stimuli under calorie restriction, independent of caloric content. To test this, we study mice fed 40% of their average daily intake and exposed daily to the non-caloric sweetener saccharin before feeding. We show that calorie-restricted (CR) mice increase saccharin intake before each restricted feeding event and that this response requires ARH integrity. CR mice exhibit activation of AgRP neurons and their brain targets without significant changes in AgRP fiber density. Furthermore, satiated mice increase saccharin intake following chemogenetic activation of AgRP neurons, whereas CR mice with selective chemogenetic inhibition of AgRP neurons show reduced saccharin intake. Thus, we conclude that AgRP neuron activation enhances the consumption of a purely rewarding stimulus in CR mice. These findings contribute to our understanding of how the brain shapes food choices under conditions of energy deficit and could be important for managing food consumption during dieting or in eating disorders.

2025-08-04T00:00:00Z Hunger enhances the consumption of rewarding foods, but the neurobiological basis of this adaptation remains unclear. We hypothesize that agouti-related protein (AgRP) neurons in the hypothalamic arcuate nucleus (ARH) promote the consumption of rewarding stimuli under calorie restriction, independent of caloric content. To test this, we study mice fed 40% of their average daily intake and exposed daily to the non-caloric sweetener saccharin before feeding. We show that calorie-restricted (CR) mice increase saccharin intake before each restricted feeding event and that this response requires ARH integrity. CR mice exhibit activation of AgRP neurons and their brain targets without significant changes in AgRP fiber density. Furthermore, satiated mice increase saccharin intake following chemogenetic activation of AgRP neurons, whereas CR mice with selective chemogenetic inhibition of AgRP neurons show reduced saccharin intake. Thus, we conclude that AgRP neuron activation enhances the consumption of a purely rewarding stimulus in CR mice. These findings contribute to our understanding of how the brain shapes food choices under conditions of energy deficit and could be important for managing food consumption during dieting or in eating disorders. Alfaro Gómez, Emma Laura Albeck, María Ester Peña Aguilera, D.C. Jurado Medina, Laura Smeldy Miguez, Linda Jezabel Cuello, Mariela Vanesa Motti, Josefina María Brenda Bravi, Claudio Marcelo Bailliet, Graciela http://sedici.unlp.edu.ar:80/handle/10915/191676 2026-03-10T20:20:46Z 2019-01-01T00:00:00Z

Objeto de conferencia XX Congreso Nacional de Arqueología Argentina (CNAA) (Córdoba, 15 al 19 de julio de 2019); Libro de resúmenes XX Congreso Nacional de Arqueología Argentina. 50 años de arqueologías Los datos arqueológicos y referencias coloniales plantean que el sector central de la Puna de Jujuy estuvo ocupado por casabindos y cochinocas. En la actualidad, se los considera como grupos con una identidad particular, que los distingue de los que habitaron los grandes valles del Noroeste Argentino desde Salta hasta el norte de San Juan (pueblos que compartieron entre sí una base lingüística y una tradición cultural, ajenas a las sociedades más septentrionales). De la misma manera los grupos de la puna se diferencian también de los de Quebrada de Humahuaca, sur de Bolivia y Atacama. Los cochinocas fueron, aparentemente, los pobladores originales del Pucará de Rinconada y como tales tendrían una antigüedad que se remontaría a mediados del primer milenio después de Cristo o aún más remota en el tiempo. Los casabindos, en cambio, presentan claras raíces altiplánicas en varios aspectos de su cultura material y su presencia en la Puna de Jujuy correspondería a los inicios del segundo milenio de la era. Desde el siglo XVII ambos grupos fueron integrados en una única encomienda y estuvieron estrechamente vinculados durante toda la dominación hispana hasta las guerras de la independencia.

2019-01-01T00:00:00Z Los datos arqueológicos y referencias coloniales plantean que el sector central de la Puna de Jujuy estuvo ocupado por casabindos y cochinocas. En la actualidad, se los considera como grupos con una identidad particular, que los distingue de los que habitaron los grandes valles del Noroeste Argentino desde Salta hasta el norte de San Juan (pueblos que compartieron entre sí una base lingüística y una tradición cultural, ajenas a las sociedades más septentrionales). De la misma manera los grupos de la puna se diferencian también de los de Quebrada de Humahuaca, sur de Bolivia y Atacama. Los cochinocas fueron, aparentemente, los pobladores originales del Pucará de Rinconada y como tales tendrían una antigüedad que se remontaría a mediados del primer milenio después de Cristo o aún más remota en el tiempo. Los casabindos, en cambio, presentan claras raíces altiplánicas en varios aspectos de su cultura material y su presencia en la Puna de Jujuy correspondería a los inicios del segundo milenio de la era. Desde el siglo XVII ambos grupos fueron integrados en una única encomienda y estuvieron estrechamente vinculados durante toda la dominación hispana hasta las guerras de la independencia. Paz Sepúlveda, Paula Beatriz Jurado Medina, Laura Smeldy Ramallo, Virginia Alfaro Gómez, Emma Laura Dipierri, José Edgardo Bravi, Claudio Marcelo Salceda, Susana Alicia Bailliet, Graciela http://sedici.unlp.edu.ar:80/handle/10915/191652 2026-03-10T20:20:47Z 2019-01-01T00:00:00Z

Objeto de conferencia XX Congreso Nacional de Arqueología Argentina (CNAA) (Córdoba, 15 al 19 de julio de 2019); Libro de resúmenes XX Congreso Nacional de Arqueología Argentina. 50 años de arqueologías Siendo el Gran Chaco uno de los ámbitos más recientemente poblado de las Tierras Bajas sudamericanas y más tardíamente colonizado, habiendo mantenido diversidad étnolingüística con evidencias de contacto interétnico, resulta ser hoy un laboratorio territorial con especiales perspectivas para un abordaje multidisciplinar integrado. Desde esta perspectiva nuestro trabajo se propone reconocer estructuración en la fracción nativa de los linajes paternos que dé cuenta de la dinámica poblacional y de patrones de distribución, que aporten elementos clarificadores de la compleja configuración de las poblaciones chaqueñas. Este trabajo se integra al proyecto multidisciplinario "De las historias étnicas a la prehistoria en el Gran Chaco” e involucra el estudio de individuos de filiación étnica wichi, toba, chorote, mocoví, lengua y ayoreo de Argentina y Paraguay.

2019-01-01T00:00:00Z Siendo el Gran Chaco uno de los ámbitos más recientemente poblado de las Tierras Bajas sudamericanas y más tardíamente colonizado, habiendo mantenido diversidad étnolingüística con evidencias de contacto interétnico, resulta ser hoy un laboratorio territorial con especiales perspectivas para un abordaje multidisciplinar integrado. Desde esta perspectiva nuestro trabajo se propone reconocer estructuración en la fracción nativa de los linajes paternos que dé cuenta de la dinámica poblacional y de patrones de distribución, que aporten elementos clarificadores de la compleja configuración de las poblaciones chaqueñas. Este trabajo se integra al proyecto multidisciplinario "De las historias étnicas a la prehistoria en el Gran Chaco” e involucra el estudio de individuos de filiación étnica wichi, toba, chorote, mocoví, lengua y ayoreo de Argentina y Paraguay. Sosa, Fabiana E. Anello, Melina Ferreyra, Hebe del Valle Medina, Diego M. Micheloud, Juan Francisco Borsetti, Hugo M. Hernández, Nancy Florin-Christensen, Mónica Romero, Sandra R. http://sedici.unlp.edu.ar:80/handle/10915/181850 2025-07-15T20:13:32Z 2025-01-01T00:00:00Z

Articulo International Journal for Parasitology: Parasites and Wildlife; vol. 27 Sarcoptic mange, caused by the mite Sarcoptes scabiei, is a highly contagious and potentially fatal skin disease that affects a wide range of mammals, including South American Camelids (SAC). Although the presence of mange has been described in vicu˜nas and llamas in northwestern Argentina, there are no previous records documenting its presence in guanacos (Lama guanicoe) of this region. We here describe a case of S. scabiei in a free-ranging guanaco in the Department of Tilcara, Jujuy Province. The animal presented alopecic, erythematous and hyperkeratotic lesions with abundant crusts in the ventral region of the body and limbs. Histopathology revealed periadnexal dermatitis with inflammatory infiltrate, severe hyperemia, orthokeratotic and parakeratotic epidermal hyperplasia, and stratum corneum thickening. The etiological agent was identified as S. scabiei by microscopic examination of adult mites. This result was confirmed by sequencing of a cytochrome oxidase subunit I gene fragment, that showed 100 % identity with sequences of isolates from SAC and other mammals. Genotyping of three mites isolated from different parts of the guanaco’s body using a set of ten microsatellite markers indicated the infection with a single genetic variant that showed a similar profile to those found in S. scabiei isolates from vicu˜nas and llamas of the same region. However, genetic differences with guanaco isolates from midwestern Argentina were observed. This work presents the first record of sarcoptic mange in a guanaco in Jujuy Province and in the northwestern region of Argentina, as well as the molecular characterization of the etiological agent. The case highlights an uncertain scenario regarding the health situation of the guanaco population in this region, which is small, fragmented, and locally categorized as endangered. Epidemiological surveillance programs for guanacos and further research on the impact of sarcoptic mange on the conservation of this species are needed.

2025-01-01T00:00:00Z Sarcoptic mange, caused by the mite Sarcoptes scabiei, is a highly contagious and potentially fatal skin disease that affects a wide range of mammals, including South American Camelids (SAC). Although the presence of mange has been described in vicu˜nas and llamas in northwestern Argentina, there are no previous records documenting its presence in guanacos (Lama guanicoe) of this region. We here describe a case of S. scabiei in a free-ranging guanaco in the Department of Tilcara, Jujuy Province. The animal presented alopecic, erythematous and hyperkeratotic lesions with abundant crusts in the ventral region of the body and limbs. Histopathology revealed periadnexal dermatitis with inflammatory infiltrate, severe hyperemia, orthokeratotic and parakeratotic epidermal hyperplasia, and stratum corneum thickening. The etiological agent was identified as S. scabiei by microscopic examination of adult mites. This result was confirmed by sequencing of a cytochrome oxidase subunit I gene fragment, that showed 100 % identity with sequences of isolates from SAC and other mammals. Genotyping of three mites isolated from different parts of the guanaco’s body using a set of ten microsatellite markers indicated the infection with a single genetic variant that showed a similar profile to those found in S. scabiei isolates from vicu˜nas and llamas of the same region. However, genetic differences with guanaco isolates from midwestern Argentina were observed. This work presents the first record of sarcoptic mange in a guanaco in Jujuy Province and in the northwestern region of Argentina, as well as the molecular characterization of the etiological agent. The case highlights an uncertain scenario regarding the health situation of the guanaco population in this region, which is small, fragmented, and locally categorized as endangered. Epidemiological surveillance programs for guanacos and further research on the impact of sarcoptic mange on the conservation of this species are needed. Sosa, Ayelen Morena Cottet, Celeste Berin, Belén Martínez, Luis Peltzer, Mercedes Prieto, María Jimena Martinez, Carolina Soledad http://sedici.unlp.edu.ar:80/handle/10915/181748 2025-07-14T20:26:51Z 2025-03-01T00:00:00Z

Articulo Pharmaceutics; vol. 17, no. 3 Background: Natural biopolymeric matrices for developing dressings have been extensively studied, as they may exhibit beneficial properties for wound healing. Gelatin possesses promising structural and physicochemical properties for incorporating active compounds (ACs). O/W emulsions are an alternative delivery system for AC with different properties and solubilities, promoting wound healing. Objective: This study aimed to develop gelatin films by adding silver nanoparticles and healing agents encapsulated in an O/W emulsion to treat skin wounds. Methods: A film-forming dispersion was prepared using gelatin and glycerol as a plasticizer, and films were obtained using the casting technique. Emulsions with ACs (EAs) and without ACs (ECs) were incorporated into the films. The formulations were analyzed by FESEM and characterized for their mechanical, thermal, and swelling properties, as well as their water vapor permeability. Results: The results showed that films with a higher amount of emulsion exhibited greater structural rigidity and lower permeability, while films with lower amounts of emulsion demonstrated more elasticity and higher permeability. General and organ-specific toxicity were evaluated in zebrafish larvae. The films showed no lethal or sub-lethal effects on the morphology or activity of the brain, heart, and liver. Conclusions: The active films developed could provide stable support and a safe delivery system for active compounds to treat skin lesions, minimizing the risk of infection and the need to heal a wound.

2025-03-01T00:00:00Z Background: Natural biopolymeric matrices for developing dressings have been extensively studied, as they may exhibit beneficial properties for wound healing. Gelatin possesses promising structural and physicochemical properties for incorporating active compounds (ACs). O/W emulsions are an alternative delivery system for AC with different properties and solubilities, promoting wound healing. Objective: This study aimed to develop gelatin films by adding silver nanoparticles and healing agents encapsulated in an O/W emulsion to treat skin wounds. Methods: A film-forming dispersion was prepared using gelatin and glycerol as a plasticizer, and films were obtained using the casting technique. Emulsions with ACs (EAs) and without ACs (ECs) were incorporated into the films. The formulations were analyzed by FESEM and characterized for their mechanical, thermal, and swelling properties, as well as their water vapor permeability. Results: The results showed that films with a higher amount of emulsion exhibited greater structural rigidity and lower permeability, while films with lower amounts of emulsion demonstrated more elasticity and higher permeability. General and organ-specific toxicity were evaluated in zebrafish larvae. The films showed no lethal or sub-lethal effects on the morphology or activity of the brain, heart, and liver. Conclusions: The active films developed could provide stable support and a safe delivery system for active compounds to treat skin lesions, minimizing the risk of infection and the need to heal a wound. Gambaro, Sabrina Eliana Zubiría, María Guillermina Giordano, Alejandra Castro, Patricia F. Garraza, Carolina Harnichar, Alejandro Ezequiel Alzamendi, Ana Spinedi, Eduardo Julio Giovambattista, Andrés http://sedici.unlp.edu.ar:80/handle/10915/167624 2024-07-01T20:09:34Z 2024-01-01T00:00:00Z

Articulo International Journal of Molecular Sciences; vol. 25, no. 3 Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement in carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little is known about the relationship between SPX and white adipose tissue (WAT) thermogenesis. Therefore, the aim of the present study was to evaluate the role of SPX in this process. C57BL/6J male mice were treated or not with SPX for ten days. On day 3, mice were randomly divided into two groups: one kept at room temperature and the other kept at cold temperature (4 ◦C). Caloric intake and body weight were recorded daily. At the end of the protocol, plasma, abdominal (epididymal), subcutaneous (inguinal), and brown AT (EAT, IAT, and BAT, respectively) depots were collected for measurements. We found that SPX treatment reduced Uncoupling protein 1 levels inWAT under both basal and cold conditions. SPX also reduced cox8b and pgc1α mRNA levels and mitochondrial DNA, principally in IAT. SPX did not modulate the number of beige precursors. SPX decreased spx levels in IAT depots and galr2 in WAT depots. No differences were observed in the BAT depots. In conclusion, we showed, for the first time, that SPX treatment in vivo reduced the thermogenic process in subcutaneous and abdominal AT, being more evident under cold stimulation.

2024-01-01T00:00:00Z Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement in carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little is known about the relationship between SPX and white adipose tissue (WAT) thermogenesis. Therefore, the aim of the present study was to evaluate the role of SPX in this process. C57BL/6J male mice were treated or not with SPX for ten days. On day 3, mice were randomly divided into two groups: one kept at room temperature and the other kept at cold temperature (4 ◦C). Caloric intake and body weight were recorded daily. At the end of the protocol, plasma, abdominal (epididymal), subcutaneous (inguinal), and brown AT (EAT, IAT, and BAT, respectively) depots were collected for measurements. We found that SPX treatment reduced Uncoupling protein 1 levels inWAT under both basal and cold conditions. SPX also reduced cox8b and pgc1α mRNA levels and mitochondrial DNA, principally in IAT. SPX did not modulate the number of beige precursors. SPX decreased spx levels in IAT depots and galr2 in WAT depots. No differences were observed in the BAT depots. In conclusion, we showed, for the first time, that SPX treatment in vivo reduced the thermogenic process in subcutaneous and abdominal AT, being more evident under cold stimulation. Giordano, Alejandra Gambaro, Sabrina Eliana Alzamendi, Ana Harnichar, Alejandro Ezequiel Rey, María Amanda Ongaro, Luisina Spinedi, Eduardo Julio Zubiría, María Guillermina Giovambattista, Andrés http://sedici.unlp.edu.ar:80/handle/10915/167351 2024-06-18T20:10:32Z 2024-01-01T00:00:00Z

Articulo International Journal of Molecular Sciences; vol. 25, no. 5 White adipose tissue (WAT) regulates energy balance through energy storage, adipokines secretion and the thermogenesis process. Beige adipocytes are responsible forWAT thermogenesis. They are generated by adipogenesis or transdifferentiation during cold or β3-adrenergic agonist stimulus through a process called browning. Browning has gained significant interest for to its preventive effect on obesity. Glucocorticoids (GCs) have several functions in WAT biology; however, their role in beige adipocyte generation and WAT browning is not fully understood. The aim of our study was to determine the effect of dexamethasone (DXM) on WAT thermogenesis. For this purpose, rats were treated with DXM at room temperature (RT) or cold conditions to determine different thermogenic markers. Furthermore, the effects of DXM on the adipogenic potential of beige precursors and on mature beige adipocytes were evaluated in vitro. Our results showed that DXM decreased UCP-1 mRNA and protein levels, mainly after cold exposure. In vitro studies showed that DXM decreased the expression of a beige precursor marker (Ebf2), affecting their ability to differentiate into beige adipocytes, and inhibited the thermogenic response of mature beige adipocytes (Ucp-1, Dio2 and Pgc1α gene expressions and mitochondrial respiration). Overall, our data strongly suggest that DXM can inhibit the thermogenic program of both retroperitoneal and inguinalWAT depots, an effect that could be exerted, at least partially, by inhibiting de novo cell generation and the thermogenic response in beige adipocytes.

2024-01-01T00:00:00Z White adipose tissue (WAT) regulates energy balance through energy storage, adipokines secretion and the thermogenesis process. Beige adipocytes are responsible forWAT thermogenesis. They are generated by adipogenesis or transdifferentiation during cold or β3-adrenergic agonist stimulus through a process called browning. Browning has gained significant interest for to its preventive effect on obesity. Glucocorticoids (GCs) have several functions in WAT biology; however, their role in beige adipocyte generation and WAT browning is not fully understood. The aim of our study was to determine the effect of dexamethasone (DXM) on WAT thermogenesis. For this purpose, rats were treated with DXM at room temperature (RT) or cold conditions to determine different thermogenic markers. Furthermore, the effects of DXM on the adipogenic potential of beige precursors and on mature beige adipocytes were evaluated in vitro. Our results showed that DXM decreased UCP-1 mRNA and protein levels, mainly after cold exposure. In vitro studies showed that DXM decreased the expression of a beige precursor marker (Ebf2), affecting their ability to differentiate into beige adipocytes, and inhibited the thermogenic response of mature beige adipocytes (Ucp-1, Dio2 and Pgc1α gene expressions and mitochondrial respiration). Overall, our data strongly suggest that DXM can inhibit the thermogenic program of both retroperitoneal and inguinalWAT depots, an effect that could be exerted, at least partially, by inhibiting de novo cell generation and the thermogenic response in beige adipocytes. Aguayo Frías, E.T. Maza Vega, Daniela Calienni, M.N. Melillo, Claudia Marisa Vazquez, D. S. Alonso, Silvia del Valle Montanari, Jorge http://sedici.unlp.edu.ar:80/handle/10915/160423 2023-11-22T20:07:00Z 2023-01-01T00:00:00Z

Articulo OpenNano; vol. 14 Vismodegib, first approved in 2012 for the treatment of basal cell carcinoma, is an inhibitor of the Hedgehog signaling pathway that becomes active in certain tumors. However, its secondary effects after oral administration and systemic distribution are severe. In this study, we loaded vismodegib into conventional liposomes, which are typically unable to penetrate the stratum corneum barrier effectively after topical application. We studied its skin penetration when coadministered with empty ethosomes, aimed at transiently disrupting the skin impermeability. The drug was successfully recovered from the deeper viable epidermal layers in an in vitro model. The preparation method for the liposomal formulation is reproducible and relatively straightforward to scale up. Furthermore, it involves the use of biocompatible lipids, thus avoiding the utilization of potentially risky compounds.

2023-01-01T00:00:00Z Vismodegib, first approved in 2012 for the treatment of basal cell carcinoma, is an inhibitor of the Hedgehog signaling pathway that becomes active in certain tumors. However, its secondary effects after oral administration and systemic distribution are severe. In this study, we loaded vismodegib into conventional liposomes, which are typically unable to penetrate the stratum corneum barrier effectively after topical application. We studied its skin penetration when coadministered with empty ethosomes, aimed at transiently disrupting the skin impermeability. The drug was successfully recovered from the deeper viable epidermal layers in an in vitro model. The preparation method for the liposomal formulation is reproducible and relatively straightforward to scale up. Furthermore, it involves the use of biocompatible lipids, thus avoiding the utilization of potentially risky compounds. Martín, Florencia M. Alzamendi, Ana Harnichar, Alejandro Ezequiel Castrogiovanni, Daniel Cayetano Zubiría, María Guillermina Spinedi, Eduardo Julio Giovambattista, Andrés http://sedici.unlp.edu.ar:80/handle/10915/159808 2023-11-06T20:06:59Z 2023-01-01T00:00:00Z

Articulo Life Sciences; vol. 322 Aim: Glucocorticoids (GCs) play a crucial role in energy homeostasis including white adipose tissue function; however, chronic GC excess is detrimental to mammals' health. White hypertrophic adiposity is a main factor for neuroendocrine-metabolic dysfunctions in monosodium L-glutamate (MSG)-damaged hypercorticosteronemic rat. Nevertheless, little is known about the receptor path in endogenous GC impact on white adipose tissueresident precursor cells to bring them into beige lineage. Thus, our aim was to explore whether transient/ chronic endogenous hypercorticosteronemia affects browning capacity in white adipose tissue pads from MSG rats during development. Main methods: Control and MSG male rats aged 30 and 90 days were 7-day exposed to cold conditions in order to stimulate wet white epidydimal adipose tissue (wEAT) beiging capacity. This procedure was also replicated in adrenalectomized rats. Key findings: Data indicated that whereas epidydimal white adipose tissue pads from prepubertal hypercorticosteronemic rats retained full expression of GR/MR genes resulting in a drastic reduction in wEAT beiging capacity, conversely, chronic hypercorticosteronemic adult MSG rats developed down-regulation of corticoid genes (and reduced GR cytosolic mediators) in wEAT pads and consequently partially restored local beiging capacity. Finally, wEAT pads from adrenalectomized rats revealed up-regulation of GR gene accompanied by full local beiging capacity. Significance: This study strongly supports a GR-dependent inhibitory effect of GC excess on white adipose tissue browning, an issue strongly supporting a key role of GR in the non-shivering thermogenic process. As a consequence, normalizing the GC milieu could be a relevant factor to handle dysmetabolism in white hyperadipose phenotypes.

2023-01-01T00:00:00Z Aim: Glucocorticoids (GCs) play a crucial role in energy homeostasis including white adipose tissue function; however, chronic GC excess is detrimental to mammals' health. White hypertrophic adiposity is a main factor for neuroendocrine-metabolic dysfunctions in monosodium L-glutamate (MSG)-damaged hypercorticosteronemic rat. Nevertheless, little is known about the receptor path in endogenous GC impact on white adipose tissueresident precursor cells to bring them into beige lineage. Thus, our aim was to explore whether transient/ chronic endogenous hypercorticosteronemia affects browning capacity in white adipose tissue pads from MSG rats during development. Main methods: Control and MSG male rats aged 30 and 90 days were 7-day exposed to cold conditions in order to stimulate wet white epidydimal adipose tissue (wEAT) beiging capacity. This procedure was also replicated in adrenalectomized rats. Key findings: Data indicated that whereas epidydimal white adipose tissue pads from prepubertal hypercorticosteronemic rats retained full expression of GR/MR genes resulting in a drastic reduction in wEAT beiging capacity, conversely, chronic hypercorticosteronemic adult MSG rats developed down-regulation of corticoid genes (and reduced GR cytosolic mediators) in wEAT pads and consequently partially restored local beiging capacity. Finally, wEAT pads from adrenalectomized rats revealed up-regulation of GR gene accompanied by full local beiging capacity. Significance: This study strongly supports a GR-dependent inhibitory effect of GC excess on white adipose tissue browning, an issue strongly supporting a key role of GR in the non-shivering thermogenic process. As a consequence, normalizing the GC milieu could be a relevant factor to handle dysmetabolism in white hyperadipose phenotypes. Rodrigues, Pedro Velázquez, Irina Florencia Ribeiro, Julyana Simão, Filipa Amorim, António Carvalho, Elizeu F. Bravi, Claudio Marcelo Basso, Néstor Guillermo Real, Luciano Esteban Galli, Claudio González, Andrea del Carmen Gamulin, Ariana Saldutti, Romina Parolín, María Laura Gomes, Verónica Gusmão, Leonor http://sedici.unlp.edu.ar:80/handle/10915/156962 2023-08-28T20:06:36Z 2022-10-01T00:00:00Z

Articulo Genes; vol. 13, no. 10 Similar to other South American regions, Tierra del Fuego has an admixed population characterized by distinct ancestors: Native Americans who first occupied the continent, European settlers who arrived from the late 15th century onwards, and Sub-Saharan Africans who were brought to the Americas for slave labor. To disclose the paternal lineages in the current population from Tierra del Fuego, 196 unrelated males were genotyped for 23 Y-STRs and 52 Y-SNPs. Haplotype and haplogroup diversities were high, indicating the absence of strong founder or drift events. A high frequency of Eurasian haplogroups was detected (94.4%), followed by Native American (5.1%) and African (0.5%) ones. The haplogroup R was the most abundant (48.5%), with the subhaplogroup R-S116* taking up a quarter of the total dataset. Comparative analyses with other Latin American populations showed similarities with other admixed populations from Argentina. Regarding Eurasian populations, Tierra del Fuego presented similarities with Italian and Iberian populations. In an in-depth analysis of the haplogroup R-M269 and its subtypes, Tierra del Fuego displayed a close proximity to the Iberian Peninsula. The results from this study are in line with the historical records and reflect the severe demographic change led mainly by male newcomers with paternal European origin.

2022-10-01T00:00:00Z Similar to other South American regions, Tierra del Fuego has an admixed population characterized by distinct ancestors: Native Americans who first occupied the continent, European settlers who arrived from the late 15th century onwards, and Sub-Saharan Africans who were brought to the Americas for slave labor. To disclose the paternal lineages in the current population from Tierra del Fuego, 196 unrelated males were genotyped for 23 Y-STRs and 52 Y-SNPs. Haplotype and haplogroup diversities were high, indicating the absence of strong founder or drift events. A high frequency of Eurasian haplogroups was detected (94.4%), followed by Native American (5.1%) and African (0.5%) ones. The haplogroup R was the most abundant (48.5%), with the subhaplogroup R-S116* taking up a quarter of the total dataset. Comparative analyses with other Latin American populations showed similarities with other admixed populations from Argentina. Regarding Eurasian populations, Tierra del Fuego presented similarities with Italian and Iberian populations. In an in-depth analysis of the haplogroup R-M269 and its subtypes, Tierra del Fuego displayed a close proximity to the Iberian Peninsula. The results from this study are in line with the historical records and reflect the severe demographic change led mainly by male newcomers with paternal European origin. González, Rebeca Hohl, Diana María Glesmann, Laura Ángela Catanesi, Cecilia Inés http://sedici.unlp.edu.ar:80/handle/10915/154915 2023-07-04T04:52:33Z 2022-01-01T00:00:00Z

Articulo Study on variants of OPRM1 and COMT genes in a population from the province of Chaco (Argentina); Estudo de variantes dos genes OPRM1 e COMT na população da província de Chaco (Argentina) Runa; vol. 43, no. 2 Se ha demostrado que los fenotipos humanos complejos, entre ellos los referidos a ciertas patologías y respuesta a fármacos, están determinados en gran medida por secuencias codificantes del genoma que pueden presentar variaciones asociadas a la ancestría y la procedencia geográfica de los individuos o poblaciones. En Argentina, la población de la provincia de Chaco presenta una gran diversidad generada por la coexistencia de comunidades nativas y foráneas, cuya base genética en relación con fenotipos complejos no ha sido estudiada en profundidad. El presente trabajo representa una contribución al conocimiento de la diversidad genética existente en poblaciones humanas chaqueñas de diferente origen, y revela variaciones significativas en la secuencia de los genes OPRM1 y COMT, los cuales modulan la transmisión de la sensación dolorosa y la respuesta a analgésicos. En el futuro, esta información podrá ser tenida en cuenta en decisiones médicas que involucren el tratamiento y prevención del dolor en cada paciente, de acuerdo con la tendencia actual hacia la medicina genómica personalizada.; It has been demonstrated that complex human phenotypes, including those related to certain pathologies and drug response, are largely determined by genome coding sequences that may vary according to the ancestry and geographical origin of individuals or populations. In Argentina, the human population of the province of Chaco presents a great population diversity, given by the coexistence of native and foreign communities, whose genetic background, in relation to complex phenotypes, has not been studied in depth. The present work represents a contribution to the knowledge of the genetic diversity of Chaco human populations of different origin, revealing significant variation in the sequence of the OPRM1 and COMT genes, which modulate the transmission of the painful sensation and the response to analgesics. In the future, this information could be taken into account in medical decisions involving treatment and prevention of pain in each patient, leading to what is currently known as personalized genomic medicine.; Foi demonstrado que fenótipos humanos complexos, incluindo aqueles relacionados a certas patologias e respostas a drogas, são em grande parte determinados por sequências de codificação do genoma que podem apresentar variações associadas à ancestralidade e à origem geográfica das indivíduos ou populações. Na Argentina, a população humana da província do Chaco apresenta uma grande diversidade gerada pela coexistência de comunidades nativas e estrangeiras, cuja base genética em relação a fenótipos complexos não foi estudada em profundidade. O presente trabalho representa uma contribuição para o conhecimento da diversidade de populações humanas do Chaco de diferentes origens, revelando variações significativas na sequência dos genes OPRM1 e COMT, que modulam a transmissão da sensação de dor e a resposta aos analgésicos. No futuro, essa informação poderia ser levada em conta nas decisões médicas que envolvem o tratamento e a prevenção da dor de cada paciente, levando ao que atualmente é conhecido como medicina genômica personalizada.

2022-01-01T00:00:00Z Se ha demostrado que los fenotipos humanos complejos, entre ellos los referidos a ciertas patologías y respuesta a fármacos, están determinados en gran medida por secuencias codificantes del genoma que pueden presentar variaciones asociadas a la ancestría y la procedencia geográfica de los individuos o poblaciones. En Argentina, la población de la provincia de Chaco presenta una gran diversidad generada por la coexistencia de comunidades nativas y foráneas, cuya base genética en relación con fenotipos complejos no ha sido estudiada en profundidad. El presente trabajo representa una contribución al conocimiento de la diversidad genética existente en poblaciones humanas chaqueñas de diferente origen, y revela variaciones significativas en la secuencia de los genes OPRM1 y COMT, los cuales modulan la transmisión de la sensación dolorosa y la respuesta a analgésicos. En el futuro, esta información podrá ser tenida en cuenta en decisiones médicas que involucren el tratamiento y prevención del dolor en cada paciente, de acuerdo con la tendencia actual hacia la medicina genómica personalizada. It has been demonstrated that complex human phenotypes, including those related to certain pathologies and drug response, are largely determined by genome coding sequences that may vary according to the ancestry and geographical origin of individuals or populations. In Argentina, the human population of the province of Chaco presents a great population diversity, given by the coexistence of native and foreign communities, whose genetic background, in relation to complex phenotypes, has not been studied in depth. The present work represents a contribution to the knowledge of the genetic diversity of Chaco human populations of different origin, revealing significant variation in the sequence of the OPRM1 and COMT genes, which modulate the transmission of the painful sensation and the response to analgesics. In the future, this information could be taken into account in medical decisions involving treatment and prevention of pain in each patient, leading to what is currently known as personalized genomic medicine. Foi demonstrado que fenótipos humanos complexos, incluindo aqueles relacionados a certas patologias e respostas a drogas, são em grande parte determinados por sequências de codificação do genoma que podem apresentar variações associadas à ancestralidade e à origem geográfica das indivíduos ou populações. Na Argentina, a população humana da província do Chaco apresenta uma grande diversidade gerada pela coexistência de comunidades nativas e estrangeiras, cuja base genética em relação a fenótipos complexos não foi estudada em profundidade. O presente trabalho representa uma contribuição para o conhecimento da diversidade de populações humanas do Chaco de diferentes origens, revelando variações significativas na sequência dos genes OPRM1 e COMT, que modulam a transmissão da sensação de dor e a resposta aos analgésicos. No futuro, essa informação poderia ser levada em conta nas decisões médicas que envolvem o tratamento e a prevenção da dor de cada paciente, levando ao que atualmente é conhecido como medicina genômica personalizada. De Francesco, Pablo Nicolás Castrogiovanni, Daniel Cayetano Uriarte, Maia Frassa, María Victoria Agosti, Francina Raingo, Jesica Perelló, Mario Carlos http://sedici.unlp.edu.ar:80/handle/10915/154700 2023-06-26T20:07:51Z 2017-01-01T00:00:00Z

Articulo Cell and Tissue Research; vol. 369 Hypothalamic tanycytes are specialized bipolar ependymal cells that line the floor of the third ventricle. Given their strategic location, tanycytes are believed to play several key functions including being a selective barrier and controlling the amount of hypothalamic-derived factors reaching the anterior pituitary. The in vitro culture of these cells has proved to be difficult. Here, we report an improved method for the generation of primary cultures of rat hypothalamic tanycytes. Ependymal cultures were derived from tissue dissected out of the median eminence region of 10-day-old rats and cultured in a chemically defined medium containing DMEM:F12, serum albumin, insulin, transferrin and the antibiotic gentamycin. After 7 days in vitro, -30% of the cultured cells exhibited morphological features of tanycytes as observed by phase contrast or scanning electron microscopy. Tanycyte-like cells were strongly immuno-reactive for vimentin and dopamine-cAMP-regulated phospho-protein (DARPP-32) and weakly immune-reactive for glial fibrillary acidic protein. Tanycyte-like cells displayed a stable negative resting plasma membrane potential and failed to show spiking properties in response to current injections. When exposed to fluorescent beads in the culture medium, tanycyte-like cells exhibited a robust endocytosis. Thus, the present method effectively yields cultures containing tanycyte-like cells that resemble in vivo tanycytes in terms of morphologic features and molecular markers as well as electrical and endocytic activity. To our knowledge, this is the first protocol that allows the culturing of tanycyte-like cells that can be individually identified and that conserve the morphology of tanycytes in then- natural physiological environment.

2017-01-01T00:00:00Z Hypothalamic tanycytes are specialized bipolar ependymal cells that line the floor of the third ventricle. Given their strategic location, tanycytes are believed to play several key functions including being a selective barrier and controlling the amount of hypothalamic-derived factors reaching the anterior pituitary. The in vitro culture of these cells has proved to be difficult. Here, we report an improved method for the generation of primary cultures of rat hypothalamic tanycytes. Ependymal cultures were derived from tissue dissected out of the median eminence region of 10-day-old rats and cultured in a chemically defined medium containing DMEM:F12, serum albumin, insulin, transferrin and the antibiotic gentamycin. After 7 days in vitro, -30% of the cultured cells exhibited morphological features of tanycytes as observed by phase contrast or scanning electron microscopy. Tanycyte-like cells were strongly immuno-reactive for vimentin and dopamine-cAMP-regulated phospho-protein (DARPP-32) and weakly immune-reactive for glial fibrillary acidic protein. Tanycyte-like cells displayed a stable negative resting plasma membrane potential and failed to show spiking properties in response to current injections. When exposed to fluorescent beads in the culture medium, tanycyte-like cells exhibited a robust endocytosis. Thus, the present method effectively yields cultures containing tanycyte-like cells that resemble in vivo tanycytes in terms of morphologic features and molecular markers as well as electrical and endocytic activity. To our knowledge, this is the first protocol that allows the culturing of tanycyte-like cells that can be individually identified and that conserve the morphology of tanycytes in then- natural physiological environment. Figuerero, María José Trejo, Sebastián Alejandro López, Laura María Isabel http://sedici.unlp.edu.ar:80/handle/10915/154515 2025-05-09T18:54:29Z 2018-01-01T00:00:00Z

Objeto de conferencia Congreso Latinoamericano de Ingeniería y Ciencias Aplicadas (CLICAP) (San Rafael, 11, 12 y 13 de abril de 2018); Memorias del CLICAP 2018: Congreso Latinoamericano de Ingeniería y Ciencias Aplicadas La harina de soja es el subproducto más relevante en el procesamiento de esta leguminosa y contiene un alto contenido de proteínas. La hidrólisis controlada de dichas proteínas puede generar ingredientes de alto valor añadido, con mejores características nutricionales y propiedades funcionales. La preparación proteolítica denominada Af, obtenida por centrifugación del látex de Asclepias fruticosa (Asclepiadaceae) recolectado en buffer cítrico-citrato, posee una única peptidasa cisteínica de PM 23,7 kDa y pI>9,3 denominada asclepaína f, la cual ha sido clonada y expresada en Pichia pastoris. La preparación Af posee una actividad proteolítica de 1,1 Ucas/ml, alta estabilidad térmica a 37° y 45°C, un rango de pH óptimo de actividad entre 7,5 y 10,5 y ha demostrado capacidad para hidrolizar las proteínas de soja, degradando fuertemente la fracción 7S y en menor medida las globulinas 11S. El objetivo del trabajo fue evaluar las propiedades funcionales (solubilidad, capacidad de retención de agua y aceite, y espumado) de un hidrolizado de proteínas de soja obtenido con la preparación Af. Se utilizó una dispersión de concentrado de soja (conteniendo 62,1 % de proteínas determinadas por Kjeldhal) al 8% p/v y una cantidad de extracto enzimático equivalente a 8,9 Ucas por gramo de proteína, con el agregado de cisteína 20 mM. La hidrólisis se llevó a cabo a diferentes valores de pH (8, 9 y 10) durante 180 minutos a 45ºC. La actividad enzimática se detuvo por calentamiento en microondas, las suspensiones fueron centrifugadas y los pellets secados en estufa. Se realizó un blanco de reacción en las mismas condiciones, reemplazando la preparación enzimática por igual volumen de agua. Posteriormente, se determinó la concentración de proteínas solubles mediante el método de Bradford, la capacidad de retención de agua a diferentes temperaturas (5, 20 y 45°C), la retención de aceite de soja y girasol, y la formación de espuma. La capacidad de retención de agua se ensayó mezclando 1 gr de muestra con 10 ml de agua destilada a diferentes temperaturas, centrifugando las suspensiones y determinando el peso de las muestras húmedas; del mismo modo se determinó la capacidad de retención de aceite. Para ensayar la capacidad espumante se prepararon suspensiones al 1% p/v de los hidrolizados, se agitaron durante 1 minuto a temperatura ambiente y posteriormente se midió el volumen de espuma y de suspensión. El mayor grado de hidrólisis de las proteínas de soja con la preparación Af se obtuvo a pH 10 y fue del 7%. A través de dicho proceso de hidrólisis se logró incrementar la concentración de proteínas solubles un 60%, la capacidad de retención de agua a 20°C se incrementó un 71% y a 45°C un 134%, mientras que la capacidad de retención de aceite de soja a 20°C aumentó un 92% y la de girasol 73,5%. En tanto que la capacidad espumante casi no fue afectada, se detectó solo un ligero aumento (3%). Empleando la preparación Af se logró obtener un hidrolizado de proteínas de soja con propiedades funcionales particulares que podrá emplearse en el desarrollo de alimentos.

2018-01-01T00:00:00Z La harina de soja es el subproducto más relevante en el procesamiento de esta leguminosa y contiene un alto contenido de proteínas. La hidrólisis controlada de dichas proteínas puede generar ingredientes de alto valor añadido, con mejores características nutricionales y propiedades funcionales. La preparación proteolítica denominada Af, obtenida por centrifugación del látex de Asclepias fruticosa (Asclepiadaceae) recolectado en buffer cítrico-citrato, posee una única peptidasa cisteínica de PM 23,7 kDa y pI>9,3 denominada asclepaína f, la cual ha sido clonada y expresada en Pichia pastoris. La preparación Af posee una actividad proteolítica de 1,1 Ucas/ml, alta estabilidad térmica a 37° y 45°C, un rango de pH óptimo de actividad entre 7,5 y 10,5 y ha demostrado capacidad para hidrolizar las proteínas de soja, degradando fuertemente la fracción 7S y en menor medida las globulinas 11S. El objetivo del trabajo fue evaluar las propiedades funcionales (solubilidad, capacidad de retención de agua y aceite, y espumado) de un hidrolizado de proteínas de soja obtenido con la preparación Af. Se utilizó una dispersión de concentrado de soja (conteniendo 62,1 % de proteínas determinadas por Kjeldhal) al 8% p/v y una cantidad de extracto enzimático equivalente a 8,9 Ucas por gramo de proteína, con el agregado de cisteína 20 mM. La hidrólisis se llevó a cabo a diferentes valores de pH (8, 9 y 10) durante 180 minutos a 45ºC. La actividad enzimática se detuvo por calentamiento en microondas, las suspensiones fueron centrifugadas y los pellets secados en estufa. Se realizó un blanco de reacción en las mismas condiciones, reemplazando la preparación enzimática por igual volumen de agua. Posteriormente, se determinó la concentración de proteínas solubles mediante el método de Bradford, la capacidad de retención de agua a diferentes temperaturas (5, 20 y 45°C), la retención de aceite de soja y girasol, y la formación de espuma. La capacidad de retención de agua se ensayó mezclando 1 gr de muestra con 10 ml de agua destilada a diferentes temperaturas, centrifugando las suspensiones y determinando el peso de las muestras húmedas; del mismo modo se determinó la capacidad de retención de aceite. Para ensayar la capacidad espumante se prepararon suspensiones al 1% p/v de los hidrolizados, se agitaron durante 1 minuto a temperatura ambiente y posteriormente se midió el volumen de espuma y de suspensión. El mayor grado de hidrólisis de las proteínas de soja con la preparación Af se obtuvo a pH 10 y fue del 7%. A través de dicho proceso de hidrólisis se logró incrementar la concentración de proteínas solubles un 60%, la capacidad de retención de agua a 20°C se incrementó un 71% y a 45°C un 134%, mientras que la capacidad de retención de aceite de soja a 20°C aumentó un 92% y la de girasol 73,5%. En tanto que la capacidad espumante casi no fue afectada, se detectó solo un ligero aumento (3%). Empleando la preparación Af se logró obtener un hidrolizado de proteínas de soja con propiedades funcionales particulares que podrá emplearse en el desarrollo de alimentos. García, M. A. Gusmão, L. Catanesi, Cecilia Inés Penacino, G. A. Pinto, N. http://sedici.unlp.edu.ar:80/handle/10915/146962 2022-12-06T20:06:18Z 2017-01-01T00:00:00Z

Articulo Forensic Science International: Genetics Supplement Series; vol. 6 The analysis of X-chromosomal markers can be important in different situations where the application of autosomal and Y- chromosomal STR markers are not sufficient to solve the cases. Currently, the Argentine population lacks a representative database on X-chromosomal markers, regarding allele and/or haplotype frequencies, and mutation rates. The absence of this information represents an important limitation for their routine use in laboratories, preventing the achievement of a quantitative, statistically supported evaluation. In order to estimate mutation rates for the twelve X-chromosomal markers included in the Argus X-12 kit, 345 father-daughter duos were genotyped. The samples were selected from all provinces of Argentina and the biological relationship of paternity was previously confirmed by the analysis of autosomal STR markers for all duos, for which likelihood ratios higher than 106 were achieved. A total of 21 mutations over 4140 allelic transmissions were observed at DXS7132, DXS10134, DXS10079, DXS10146, DXS10101, DXS10103, DXS10074, DXS10148 and DXS10135 loci. The overall X-STRmutation rate observed was 5.1×10−3 (95% CI, 3.1×10−3–7.7 ×10−3) and all the genotypic configurations were explainable by the gain or loss of a single repeat. Finally, it should be noted that the overall mutation rate observed in this work resulted higher in comparison with some other reports, likely due to only father-daughter duos had been considered. Indeed, these findings are in agreement with previous works suggesting higher mutation rates for males, due to the higher number of germline divisions they experience.

2017-01-01T00:00:00Z The analysis of X-chromosomal markers can be important in different situations where the application of autosomal and Y- chromosomal STR markers are not sufficient to solve the cases. Currently, the Argentine population lacks a representative database on X-chromosomal markers, regarding allele and/or haplotype frequencies, and mutation rates. The absence of this information represents an important limitation for their routine use in laboratories, preventing the achievement of a quantitative, statistically supported evaluation. In order to estimate mutation rates for the twelve X-chromosomal markers included in the Argus X-12 kit, 345 father-daughter duos were genotyped. The samples were selected from all provinces of Argentina and the biological relationship of paternity was previously confirmed by the analysis of autosomal STR markers for all duos, for which likelihood ratios higher than 106 were achieved. A total of 21 mutations over 4140 allelic transmissions were observed at DXS7132, DXS10134, DXS10079, DXS10146, DXS10101, DXS10103, DXS10074, DXS10148 and DXS10135 loci. The overall X-STRmutation rate observed was 5.1×10−3 (95% CI, 3.1×10−3–7.7 ×10−3) and all the genotypic configurations were explainable by the gain or loss of a single repeat. Finally, it should be noted that the overall mutation rate observed in this work resulted higher in comparison with some other reports, likely due to only father-daughter duos had been considered. Indeed, these findings are in agreement with previous works suggesting higher mutation rates for males, due to the higher number of germline divisions they experience.