http://sedici.unlp.edu.ar:80/handle/10915/130 Tue, 20 Oct 2026 13:58:39 GMT 2026-10-20T13:58:39Z http://sedici.unlp.edu.ar:80/handle/10915/6600 Contribucion a revista A pedagogical structure for the education of the epidemiology in the Residence in Clinical Pharmacy Acta Farmacéutica Bonaerense; vol. 22, no. 3 Se presenta una estrategia pedagógica para la enseñanza de la epidemiología en el marco de las Residencias Públicas en Farmacia Clínica. Una década de experiencia curricular aplicada a la fármaco-epidemiología ha permitido concluir que el mejor abordaje para anclar los conceptos epidemiológicos es adentrase en las condiciones estructurales del sistema sanitario desde el cual se opera, rescatando la visión y misión de los fundadores de cada casa y rescatando el aporte de organizaciones que operan por fuera de la estructura hospitalaria en sí.; A pedagogic strategy for epidemiological education in Clinical Pharmacy Residence is introduced. One decade of curricular experience applied to drug epidemiology has allowed to conclude that the best boarding to anchor the epidemiological concepts is to search deeper in the structural conditions of the sanitary system from which it is operating, rescuing the vision and mission of the founders of each house and the contribution of organizations who act outside the hospital structure. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6600 2003-01-01T00:00:00Z Se presenta una estrategia pedagógica para la enseñanza de la epidemiología en el marco de las Residencias Públicas en Farmacia Clínica. Una década de experiencia curricular aplicada a la fármaco-epidemiología ha permitido concluir que el mejor abordaje para anclar los conceptos epidemiológicos es adentrase en las condiciones estructurales del sistema sanitario desde el cual se opera, rescatando la visión y misión de los fundadores de cada casa y rescatando el aporte de organizaciones que operan por fuera de la estructura hospitalaria en sí. A pedagogic strategy for epidemiological education in Clinical Pharmacy Residence is introduced. One decade of curricular experience applied to drug epidemiology has allowed to conclude that the best boarding to anchor the epidemiological concepts is to search deeper in the structural conditions of the sanitary system from which it is operating, rescuing the vision and mission of the founders of each house and the contribution of organizations who act outside the hospital structure. http://sedici.unlp.edu.ar:80/handle/10915/6599 Articulo Medicinal plants and phytotherapy: dependence indicators or development issues? Acta Farmacéutica Bonaerense; vol. 22, no. 3 Los medicamentos fitoterápicos están constituidos por ingredientes activos de origen vegetal formulados bajo la forma farmacéutica más adecuada para su administración. La posibilidad de utilización de la Fitoterapia en las prácticas terapéuticas, con sustento científico, exige acciones multisectoriales que involucren desde la producción primaria de plantas medicinales hasta el establecimiento de los procesos de control de calidad de las materias primas y medicamentos. La Fitoterapia podría transformarse entonces, de ser actualmente un indicador de dependencia, en un factor real de desarrollo para la agroindustria farmacéutica de Latinoamérica, si fuera encarada por las políticas de ciencia y tecnología regionales y nacionales con un criterio más moderno y ecuánime, y en consonancia con las necesidades de la población.; Phytomedicines are constituted exclusively by active principles from plant origin formulated under the best pharmaceutical form adapted for their administration. The application of Phytotherapy in therapeutic practices, with scientific support, demands multisectorial actions that involve from the primary production of medicinal plants to the establishment of quality control processes of raw materials and medicines. Once accomplished these objectives, Phytoterapy could become, from a dependency indicator in a real development factor for the pharmaceutical agroindustry in Latin America, if outside faced by the regional and national policies of science and technology with a more modern and eventempered criterion, and in agreement with the population needs. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6599 2003-01-01T00:00:00Z Los medicamentos fitoterápicos están constituidos por ingredientes activos de origen vegetal formulados bajo la forma farmacéutica más adecuada para su administración. La posibilidad de utilización de la Fitoterapia en las prácticas terapéuticas, con sustento científico, exige acciones multisectoriales que involucren desde la producción primaria de plantas medicinales hasta el establecimiento de los procesos de control de calidad de las materias primas y medicamentos. La Fitoterapia podría transformarse entonces, de ser actualmente un indicador de dependencia, en un factor real de desarrollo para la agroindustria farmacéutica de Latinoamérica, si fuera encarada por las políticas de ciencia y tecnología regionales y nacionales con un criterio más moderno y ecuánime, y en consonancia con las necesidades de la población. Phytomedicines are constituted exclusively by active principles from plant origin formulated under the best pharmaceutical form adapted for their administration. The application of Phytotherapy in therapeutic practices, with scientific support, demands multisectorial actions that involve from the primary production of medicinal plants to the establishment of quality control processes of raw materials and medicines. Once accomplished these objectives, Phytoterapy could become, from a dependency indicator in a real development factor for the pharmaceutical agroindustry in Latin America, if outside faced by the regional and national policies of science and technology with a more modern and eventempered criterion, and in agreement with the population needs. http://sedici.unlp.edu.ar:80/handle/10915/6598 Articulo Oxidative stress in infection by human T-lymphotropic virus type I Acta Farmacéutica Bonaerense; vol. 22, no. 3 El virus linfo-trópico de células T humano de tipo I (HTLV-I) es un retrovirus asociado entre otras enfermedades a la leucemia de células T del adulto y la paraparesia espástica tropical. La patología es aparentemente el resultado de la actividad de la proteína viral Tax que participa en el incremento de la producción de especies reactivas de oxígeno requeridas para la activación de NF-KB, y que transactiva la expresión de genes virales y celulares que resulta en la transformación celular, la inmortalización y la enfermedad. La caracterización de dichos mecanismos contribuye a una mejor comprensión de la fisiopatología de la infección y en la perspectiva de intervención terapéutica preventiva.; The human T-lymphotropic virus type I is a retrovirus associated to adult T cell leukemia, the neurological disorder tropical spastic paraparesis and other related diseases. Pathology apparently results from the activities of the Tax viral protein, which increase the generation of oxidative stress species required for NF-KB activation, and trans-activates the expression of viral and cellular genes. This results in cell transformation, immortalization and disease. The characterization of these mechanisms might contribute both to better understand infection physiology and to the perspective of preventive therapeutic intervention. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6598 2003-01-01T00:00:00Z El virus linfo-trópico de células T humano de tipo I (HTLV-I) es un retrovirus asociado entre otras enfermedades a la leucemia de células T del adulto y la paraparesia espástica tropical. La patología es aparentemente el resultado de la actividad de la proteína viral Tax que participa en el incremento de la producción de especies reactivas de oxígeno requeridas para la activación de NF-KB, y que transactiva la expresión de genes virales y celulares que resulta en la transformación celular, la inmortalización y la enfermedad. La caracterización de dichos mecanismos contribuye a una mejor comprensión de la fisiopatología de la infección y en la perspectiva de intervención terapéutica preventiva. The human T-lymphotropic virus type I is a retrovirus associated to adult T cell leukemia, the neurological disorder tropical spastic paraparesis and other related diseases. Pathology apparently results from the activities of the Tax viral protein, which increase the generation of oxidative stress species required for NF-KB activation, and trans-activates the expression of viral and cellular genes. This results in cell transformation, immortalization and disease. The characterization of these mechanisms might contribute both to better understand infection physiology and to the perspective of preventive therapeutic intervention. http://sedici.unlp.edu.ar:80/handle/10915/6597 Articulo Aminosalicylates: characteristics of the oral formulations used in the treatment of inflammatory bowel disease Acta Farmacéutica Bonaerense; vol. 22, no. 3 Los derivados del ácido 5-aminosalicílico (5-ASA) son una importante alternativa terapéutica para muchos pacientes con enfermedad inflamatoria intestinal. Se han desarrollado nuevas formulaciones de 5-ASA con el objeto de lograr la liberación del fármaco en diferentes sitios del tracto gastrointestinal, tales como mesalazina, balsalazida y olsalazina. Se describen las características de las drogas con 5-ASA y sus efectos adversos.; The 5-aminosalicyc acid (5-ASA) derivatives are an important therapeutic option for patients with inflammatory bowel disease. New formulations of 5-ASA have been developed with the object of achieving the releasing of the drug in different sites of gastrointestinal tract, such as mesalazine, balsalazide and olsalazine. It is described the characteristics of the drug with 5-ASA and its side effects. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6597 2003-01-01T00:00:00Z Los derivados del ácido 5-aminosalicílico (5-ASA) son una importante alternativa terapéutica para muchos pacientes con enfermedad inflamatoria intestinal. Se han desarrollado nuevas formulaciones de 5-ASA con el objeto de lograr la liberación del fármaco en diferentes sitios del tracto gastrointestinal, tales como mesalazina, balsalazida y olsalazina. Se describen las características de las drogas con 5-ASA y sus efectos adversos. The 5-aminosalicyc acid (5-ASA) derivatives are an important therapeutic option for patients with inflammatory bowel disease. New formulations of 5-ASA have been developed with the object of achieving the releasing of the drug in different sites of gastrointestinal tract, such as mesalazine, balsalazide and olsalazine. It is described the characteristics of the drug with 5-ASA and its side effects. http://sedici.unlp.edu.ar:80/handle/10915/6596 Articulo Tretinoin/isotretinoin interconversion: a problem in preparing pharmaceutical formulations Acta Farmacéutica Bonaerense; vol. 22, no. 3 O ácido retinóico ou tretinoína, é usado topicamente no tratamento da acne e da pele fotodanificada. O seu isômero 13-cis, a isotretinoína, possui ação antiqueratinizante e é administrada por via oral. Ambos são reconhecidamente sensíveis à luz. O objetivo deste trabalho é avaliar a qualidade da matéria-prima de ácido retinóico utilizada em farmácias magistrais. Para tanto, foi realizada a análise farmacêutica do ácido retinóico conforme o preconizado nos códigos oficiais. Verificou-se também, quais testes de identificação conseguem seguramente diferenciá-lo de seu isômero, a isotretinoína. O teor médio obtido no doseamento do ácido retinóico pelo método oficial, volumetria em meio não-aquoso (107,0%), não atende as especificações farmacopéicas. Foram analisadas, por cromatografia líquida de alta eficiência (CLAE), soluções metanólicas dos fármacos expostas à luz ambiente. Após 25 minutos de exposição à luz restaram apenas 25,5% e 27,6% do teor inicial de ácido retinóico e isotretinoína, respectivamente. O ácido retinóico apresentou como principal produto de degradação a isotretinoína e vice-versa.; Tretinoin is applied topically in the treatment of acne. Isotretinoin, its isomer, inhibits keratinization and it is used orally. Both are light sensitive. The aim of the present work is to evaluate the purity of tretinoin (raw material) used in local pharmacies for pharmaceutical preparations. Analysis was carried out according to official codes. Tretinoin sample was found to be out of pharmacopeial specifications. Methanolic solutions of both isomers were also analysed by HPLC, in order to evaluate their photostability. After 25 minutes less than 30% of original substance remained in the solutions. Tretinoin is the main degradation product of isotretinoin and vice-versa. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6596 2003-01-01T00:00:00Z O ácido retinóico ou tretinoína, é usado topicamente no tratamento da acne e da pele fotodanificada. O seu isômero 13-cis, a isotretinoína, possui ação antiqueratinizante e é administrada por via oral. Ambos são reconhecidamente sensíveis à luz. O objetivo deste trabalho é avaliar a qualidade da matéria-prima de ácido retinóico utilizada em farmácias magistrais. Para tanto, foi realizada a análise farmacêutica do ácido retinóico conforme o preconizado nos códigos oficiais. Verificou-se também, quais testes de identificação conseguem seguramente diferenciá-lo de seu isômero, a isotretinoína. O teor médio obtido no doseamento do ácido retinóico pelo método oficial, volumetria em meio não-aquoso (107,0%), não atende as especificações farmacopéicas. Foram analisadas, por cromatografia líquida de alta eficiência (CLAE), soluções metanólicas dos fármacos expostas à luz ambiente. Após 25 minutos de exposição à luz restaram apenas 25,5% e 27,6% do teor inicial de ácido retinóico e isotretinoína, respectivamente. O ácido retinóico apresentou como principal produto de degradação a isotretinoína e vice-versa. Tretinoin is applied topically in the treatment of acne. Isotretinoin, its isomer, inhibits keratinization and it is used orally. Both are light sensitive. The aim of the present work is to evaluate the purity of tretinoin (raw material) used in local pharmacies for pharmaceutical preparations. Analysis was carried out according to official codes. Tretinoin sample was found to be out of pharmacopeial specifications. Methanolic solutions of both isomers were also analysed by HPLC, in order to evaluate their photostability. After 25 minutes less than 30% of original substance remained in the solutions. Tretinoin is the main degradation product of isotretinoin and vice-versa. http://sedici.unlp.edu.ar:80/handle/10915/6595 Articulo Métodos espectrofotométricos para la determinación de compuestos fenólicos en propóleos Acta Farmacéutica Bonaerense; vol. 22, no. 3 Se evaluaron tres métodos espectrofotométricos para la determinación de compuestos fenólicos totales en propóleos de la provincia de Tucumán, Argentina, mediante los métodos de Folin-Ciocalteau, azul de Prusia y o-fenantrolina. El método de azul de Prusia fue el más sensible aunque inestable; el método de o-fenantrolina fue bastante sensible y más estable que el anterior mientras que el de Folin-Ciocalteau fue el más estable y reproducible. Al comparar estadísticamente los tres métodos usados se encontró que a un nivel de significación del 1% los tres métodos son diferentes, salvo azul de Prusia y o-fenantrolina, en los que no hay diferencias significativas, utilizando el patrón quercetina.; Three spectrophotometric methods were evaluated in order to determine total phenolic compounds content in propolis from Tucumán, Argentina; applying the Folin-Ciocalteau, Prussian blue and o-phenanthroline methods. The Prussian blue method was the most sensitive one although it was also unstable. The o-phenanthroline method may be more reliable and proved to be quite sensitive and stable. Folin-Ciocalteau method was the most stable and reproducible of all. The statistic analysis for such methods as well as for each of the controls used showed that all the three methods were different at 1% significance levels, except for the Prussian blue and o-phenanthroline methods, which yielded no significant differences for the quercetin control solution. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6595 2003-01-01T00:00:00Z Se evaluaron tres métodos espectrofotométricos para la determinación de compuestos fenólicos totales en propóleos de la provincia de Tucumán, Argentina, mediante los métodos de Folin-Ciocalteau, azul de Prusia y o-fenantrolina. El método de azul de Prusia fue el más sensible aunque inestable; el método de o-fenantrolina fue bastante sensible y más estable que el anterior mientras que el de Folin-Ciocalteau fue el más estable y reproducible. Al comparar estadísticamente los tres métodos usados se encontró que a un nivel de significación del 1% los tres métodos son diferentes, salvo azul de Prusia y o-fenantrolina, en los que no hay diferencias significativas, utilizando el patrón quercetina. Three spectrophotometric methods were evaluated in order to determine total phenolic compounds content in propolis from Tucumán, Argentina; applying the Folin-Ciocalteau, Prussian blue and o-phenanthroline methods. The Prussian blue method was the most sensitive one although it was also unstable. The o-phenanthroline method may be more reliable and proved to be quite sensitive and stable. Folin-Ciocalteau method was the most stable and reproducible of all. The statistic analysis for such methods as well as for each of the controls used showed that all the three methods were different at 1% significance levels, except for the Prussian blue and o-phenanthroline methods, which yielded no significant differences for the quercetin control solution. http://sedici.unlp.edu.ar:80/handle/10915/6594 Articulo Toxicity study at limit dose of carbide waste in Sprague Dawley rats Acta Farmacéutica Bonaerense; vol. 22, no. 3 El cieno de acetileno se ha venido utilizando en los últimos años en diversas aplicaciones biológicas y se han realizado diversas pruebas para valorar su posible toxicidad. El objetivo del presente trabajo es profundizar en los aspectos toxicológicos de este residual El trabajo se desarrolló en la UTEX del ISCM-VC. Se utilizaron 30 ratas Sprague Dawley (15 por sexo) de 6 a 7 semanas de edad. Para cada sexo se conformaron 3 grupos: 1) animales tratados con cieno de acetileno (2 g/Kg peso suspendido en 2 ml de aceite de girasol); 2) animales sólo con vehículo (2 ml de aceite de girasol) y 3) control negativo (se administraron 2 ml de agua destilada). Los animales se pesaron antes de comenzar el experimento y a los 7 y 14 días, momento en que se sacrificaron para su estudio anatomopatológico. A los órganos que presentaron lesiones macroscópicas se le tomaron muestras para histopatología. Durante el período experimental no se produjeron muertes. Los animales del grupo control vehículo tuvieron una ganancia media diaria significativamente inferior a los otros grupos. No se encontraron diferencias entre grupos para el peso de los órganos ni lesiones macro - microscópicas atribuibles al cieno de acetileno.; Carbide Waste has been used for several years as treatment of animal diseases and alimentary supplementation. Some assays have shown no toxic effects of this residual. The aim of this work is to go deeply in further investigation in possible toxicity of this product. The present study was carried out at the Experimental Toxicology Unit of the Higher Institute of Medical Science of Villa Clara, Cuba, to assess acetylene mud toxicity when it is used as oral supplement in laboratory rats. Thirty Sprague Dawley rats were used (15 females and 15 males, 6-7 weeks age). Three groups were formed: 1) animals treated with carbide waste (2 g/Kg body weight in 2 ml of vegetal oil); 2) vehicle control (2 ml of vegetal oil), and 3) negative control (sterile water). Animals were weighed before experiment and at 7 and 14 days later, time of slaughter, for morphological study. No animals dead during the experimental period. Treated and control animals had similar weight development and a few cases of organ lesions were detected, in similar proportion in all groups. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6594 2003-01-01T00:00:00Z El cieno de acetileno se ha venido utilizando en los últimos años en diversas aplicaciones biológicas y se han realizado diversas pruebas para valorar su posible toxicidad. El objetivo del presente trabajo es profundizar en los aspectos toxicológicos de este residual El trabajo se desarrolló en la UTEX del ISCM-VC. Se utilizaron 30 ratas Sprague Dawley (15 por sexo) de 6 a 7 semanas de edad. Para cada sexo se conformaron 3 grupos: 1) animales tratados con cieno de acetileno (2 g/Kg peso suspendido en 2 ml de aceite de girasol); 2) animales sólo con vehículo (2 ml de aceite de girasol) y 3) control negativo (se administraron 2 ml de agua destilada). Los animales se pesaron antes de comenzar el experimento y a los 7 y 14 días, momento en que se sacrificaron para su estudio anatomopatológico. A los órganos que presentaron lesiones macroscópicas se le tomaron muestras para histopatología. Durante el período experimental no se produjeron muertes. Los animales del grupo control vehículo tuvieron una ganancia media diaria significativamente inferior a los otros grupos. No se encontraron diferencias entre grupos para el peso de los órganos ni lesiones macro - microscópicas atribuibles al cieno de acetileno. Carbide Waste has been used for several years as treatment of animal diseases and alimentary supplementation. Some assays have shown no toxic effects of this residual. The aim of this work is to go deeply in further investigation in possible toxicity of this product. The present study was carried out at the Experimental Toxicology Unit of the Higher Institute of Medical Science of Villa Clara, Cuba, to assess acetylene mud toxicity when it is used as oral supplement in laboratory rats. Thirty Sprague Dawley rats were used (15 females and 15 males, 6-7 weeks age). Three groups were formed: 1) animals treated with carbide waste (2 g/Kg body weight in 2 ml of vegetal oil); 2) vehicle control (2 ml of vegetal oil), and 3) negative control (sterile water). Animals were weighed before experiment and at 7 and 14 days later, time of slaughter, for morphological study. No animals dead during the experimental period. Treated and control animals had similar weight development and a few cases of organ lesions were detected, in similar proportion in all groups. http://sedici.unlp.edu.ar:80/handle/10915/6593 Articulo Beta-lactam resistance mediated by plasmids in pseudomonas aeruginosa clinical isolates Acta Farmacéutica Bonaerense; vol. 22, no. 3 Se estudiaron 117 cepas de Pseudomonas aeruginosa con el objetivo de determinar si la resistencia a beta-lactámicos se encontraba mediada por plásmidos, realizándoles el estudio de sensibilidad in vitro frente a azlocilina, carbenicilina, cefuroxima, cefotaxima y ceftazidima. El análisis plasmídico y la producción de beta-lactamasas se llevó a cabo con las 117 cepas. El experimento de transconjungación se realizó con aquellas cepas productoras de beta-lactamasas resistentes al menos a un antibiótico. Mediante PCR se determinó la presencia de la enzima TEM. Las cepas resistentes a cefotaxima no transconjugaron, mientras que la transconjugación fue efectiva en el 100% de las cepas resistentes a los otros beta-lactámicos. Solamente las cepas transconjugantes resistentes a cefuroxima y ceftazidima fueron productoras de beta-lactamasa. Se obtuvo amplificación de los genes TEM en el 63% de las cepas resistentes a ceftazidima.; We studied 117 Pseudomonas aeruginosa strains from clinical isolates in order to determinate if the antibiotic resistance were mediated by plasmid. The antibiotic susceptibility testing was determinated by a minimal inhibitory concentration to azlocillin, carbenicillin, cefuroxime, cefotaxime and ceftazidime. Plasmid analysis and a beta-lactamase assay were performed on such clinical strains. Transconjugation experiments were done with all resistant strains regarding plasmid and betalactamase production. The presence of the TEM gene was determinated by PCR. The transconjugation event with cefotaxime resistant strains have failed. Conjugation to carbenicillin, azlocillin, cefuroxime and ceftazidime was 100% effective, but only transconjugated resistant strains to cefuroxime and ceftazidime were beta-lactamase producer. We obtained the amplification of the TEM gene in 63% of ceftazidime resistant strains. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6593 2003-01-01T00:00:00Z Se estudiaron 117 cepas de Pseudomonas aeruginosa con el objetivo de determinar si la resistencia a beta-lactámicos se encontraba mediada por plásmidos, realizándoles el estudio de sensibilidad in vitro frente a azlocilina, carbenicilina, cefuroxima, cefotaxima y ceftazidima. El análisis plasmídico y la producción de beta-lactamasas se llevó a cabo con las 117 cepas. El experimento de transconjungación se realizó con aquellas cepas productoras de beta-lactamasas resistentes al menos a un antibiótico. Mediante PCR se determinó la presencia de la enzima TEM. Las cepas resistentes a cefotaxima no transconjugaron, mientras que la transconjugación fue efectiva en el 100% de las cepas resistentes a los otros beta-lactámicos. Solamente las cepas transconjugantes resistentes a cefuroxima y ceftazidima fueron productoras de beta-lactamasa. Se obtuvo amplificación de los genes TEM en el 63% de las cepas resistentes a ceftazidima. We studied 117 Pseudomonas aeruginosa strains from clinical isolates in order to determinate if the antibiotic resistance were mediated by plasmid. The antibiotic susceptibility testing was determinated by a minimal inhibitory concentration to azlocillin, carbenicillin, cefuroxime, cefotaxime and ceftazidime. Plasmid analysis and a beta-lactamase assay were performed on such clinical strains. Transconjugation experiments were done with all resistant strains regarding plasmid and betalactamase production. The presence of the TEM gene was determinated by PCR. The transconjugation event with cefotaxime resistant strains have failed. Conjugation to carbenicillin, azlocillin, cefuroxime and ceftazidime was 100% effective, but only transconjugated resistant strains to cefuroxime and ceftazidime were beta-lactamase producer. We obtained the amplification of the TEM gene in 63% of ceftazidime resistant strains. http://sedici.unlp.edu.ar:80/handle/10915/6592 Articulo Stability study of Secnidazole 500 mg coated tablets Acta Farmacéutica Bonaerense; vol. 22, no. 3 Se realizó el estudio de estabilidad de secnidazol tabletas recubiertas de 500 mg, de producción nacional, y se determinó la fecha de vencimiento mediante el método de vida de estante. Para cuantificar el principio activo se empleó la cromatografía líquida de alta eficacia. En la técnica, debidamente validada, se empleó una columna de fase reversa RP-18, una mezcla metanol-agua (50:50, v/v) y detector ultravioleta con longitud de onda fijada a 320 nm. Se analizó el efecto de la humedad sobre la estabilidad del fármaco, mediante la colocación de muestras en hidrostatos con humedades controladas de 75, 84 y 92%. El cumplimiento de los parámetros de calidad establecidos para este medicamento, tales como características organolépticas, identificación y valoración del principio activo y el ensayo de disolución, después de transcurridos 6, 12 y 30 meses, infieren que la formulación desarrollada es estable en el período de tiempo estudiado. El estudio de la influencia de la humedad demostró que la ganancia en peso fue inferior al 2,2% y que la exposición a elevados porcentajes de humedad relativa, durante 30 días, no afecta la estabilidad del producto.; Stability testing for secnidazole 500 mg coated tablets of national production was performed and the expiration date was determined by the shelf life method. A validate high-performance liquid chromatographic method for secnidazole quantification was used. Chromatography was carried out on a RP-18 column with a methanol-water (50:50, v/v) mixture as mobile phase and wavelength fixed at 320 nm on the UV detector. The effect of the humidity over the medicine stability was analysed by means of the placement of samples in hydrostats with controlled humidity of 75, 84 and 92%. Organoleptic characteristics, secnidazole identification, determination, dissolution assay and the others quality parameters established were fulfilled during stability testing. The developed formulation was stable 30 months after its manufacture. Study of the humidity influence showed a weight gain lower to 2.2% and proved that tablets stability was not affected under high percents of relative humidity. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6592 2003-01-01T00:00:00Z Se realizó el estudio de estabilidad de secnidazol tabletas recubiertas de 500 mg, de producción nacional, y se determinó la fecha de vencimiento mediante el método de vida de estante. Para cuantificar el principio activo se empleó la cromatografía líquida de alta eficacia. En la técnica, debidamente validada, se empleó una columna de fase reversa RP-18, una mezcla metanol-agua (50:50, v/v) y detector ultravioleta con longitud de onda fijada a 320 nm. Se analizó el efecto de la humedad sobre la estabilidad del fármaco, mediante la colocación de muestras en hidrostatos con humedades controladas de 75, 84 y 92%. El cumplimiento de los parámetros de calidad establecidos para este medicamento, tales como características organolépticas, identificación y valoración del principio activo y el ensayo de disolución, después de transcurridos 6, 12 y 30 meses, infieren que la formulación desarrollada es estable en el período de tiempo estudiado. El estudio de la influencia de la humedad demostró que la ganancia en peso fue inferior al 2,2% y que la exposición a elevados porcentajes de humedad relativa, durante 30 días, no afecta la estabilidad del producto. Stability testing for secnidazole 500 mg coated tablets of national production was performed and the expiration date was determined by the shelf life method. A validate high-performance liquid chromatographic method for secnidazole quantification was used. Chromatography was carried out on a RP-18 column with a methanol-water (50:50, v/v) mixture as mobile phase and wavelength fixed at 320 nm on the UV detector. The effect of the humidity over the medicine stability was analysed by means of the placement of samples in hydrostats with controlled humidity of 75, 84 and 92%. Organoleptic characteristics, secnidazole identification, determination, dissolution assay and the others quality parameters established were fulfilled during stability testing. The developed formulation was stable 30 months after its manufacture. Study of the humidity influence showed a weight gain lower to 2.2% and proved that tablets stability was not affected under high percents of relative humidity. http://sedici.unlp.edu.ar:80/handle/10915/6591 Articulo Synthesis and evaluation of antimicrobial activity of Primin Analogues 5- and 6-alkylsubstituted Acta Farmacéutica Bonaerense; vol. 22, no. 3 Foram obtidos duas séries de compostos análogos da primina, 6- e 5-alquil-2-metoxibenzoqui-nonas. As quinonas 6-alquil-substituídas foram obtidas a partir do guaiacol em três etapas: proteção, metalação seguido de alquilação, hidrólise e subsequente oxidação catalisada por salcomina. Os isômeros 5-alquil substituídos foram obtidos a partir da alquilação seletiva da 2-metoxi-1,4-benzoquinona com trial-quilboranos e subsequente oxidação. Os produtos foram testados quanto a sua atividade antimicrobiana para a determinação da CIM contra 21 germes representativos. As quinonas 6-alquil-substituídas apresentaram uma maior inibição que as as 5-alquilsubstituídas. Em geral, os compostos foram mais ativos frente aos microorganismos gram-positivos que aos demais tipos.; Both 6- and 5-alkyl-2-methoxy-1,4-benzoquinone derivatives, analogues of primin, were obtained. To obtain the 6-alkyl substituted compound, tetrahydropyranylated guaiacol was treated with n-butyllithium followed by alkylation with alkyl bromides. Hydrolysis to the corresponding 6-substituted 2-methoxyphenols and subsequent oxidation catalyzed by salcomine afforded high yields of the quinones. 5-alkyl-2-methoxy-1,4-benzo-quinones were obtained by selective alkylation in position 5 and oxidative work up of 2-methoxy-1,4-benzo-quinone with trialkylboranes. All compounds were tested in order to evaluate the antimicrobial activity. In general, the 6-alkyl derivatives revealed to be more active than the 5-alkyl series. Some of them showed interesting antimicrobial activity selectively against gram-positive bacteria. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6591 2003-01-01T00:00:00Z Foram obtidos duas séries de compostos análogos da primina, 6- e 5-alquil-2-metoxibenzoqui-nonas. As quinonas 6-alquil-substituídas foram obtidas a partir do guaiacol em três etapas: proteção, metalação seguido de alquilação, hidrólise e subsequente oxidação catalisada por salcomina. Os isômeros 5-alquil substituídos foram obtidos a partir da alquilação seletiva da 2-metoxi-1,4-benzoquinona com trial-quilboranos e subsequente oxidação. Os produtos foram testados quanto a sua atividade antimicrobiana para a determinação da CIM contra 21 germes representativos. As quinonas 6-alquil-substituídas apresentaram uma maior inibição que as as 5-alquilsubstituídas. Em geral, os compostos foram mais ativos frente aos microorganismos gram-positivos que aos demais tipos. Both 6- and 5-alkyl-2-methoxy-1,4-benzoquinone derivatives, analogues of primin, were obtained. To obtain the 6-alkyl substituted compound, tetrahydropyranylated guaiacol was treated with n-butyllithium followed by alkylation with alkyl bromides. Hydrolysis to the corresponding 6-substituted 2-methoxyphenols and subsequent oxidation catalyzed by salcomine afforded high yields of the quinones. 5-alkyl-2-methoxy-1,4-benzo-quinones were obtained by selective alkylation in position 5 and oxidative work up of 2-methoxy-1,4-benzo-quinone with trialkylboranes. All compounds were tested in order to evaluate the antimicrobial activity. In general, the 6-alkyl derivatives revealed to be more active than the 5-alkyl series. Some of them showed interesting antimicrobial activity selectively against gram-positive bacteria. http://sedici.unlp.edu.ar:80/handle/10915/6590 Articulo Pharmacognostic analysis of Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) Yunck: botanical aspects and preliminary physical-chemical analysis Acta Farmacéutica Bonaerense; vol. 22, no. 3 Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) Yunck. (pariparoba), uma planta medicinal que ocorre no Brasil, é popularmente utilizada em casos de ulcerações e irritação da pele. Dados farmacognósticos insuficientes e a grande semelhança entre as espécies de Piper levaram a realização desde trabalho, para o estabelecimento de parâmetros de analise botânica e físico-química da referida espécie. A descrição anatômica foi realizada através de lâminas permanentes e semi-permanentes do caule, folhas e raízes, corados com azul de astra e safranina. Testes histoquímicos e farmacopéicos realizados mostraram a presença de células oleíferas e determinaram os parâmetros para avaliação da qualidade e pureza.; Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) Yunck. (pariparoba), a folk plant of occurrence in Brazil, is popularly used in several cases of ulceration and skin irritation. Owed to insufficient pharmacognostic data about the species and its similarity with other Piper species, the purpose of this work was to establish the botanical aspects and preliminary analysis of physical-chemical parameters. Anatomical description was undertaken by the analysis of semi-permanent and permanent slides made with different sections of leaves, stem and roots, stained with safranin and astra blue. The histochemical and pharmacopoeial tests demonstrated the presence of cells with lipophilic content and determined the parameters than can be used for quality control and purity degree. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6590 2003-01-01T00:00:00Z Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) Yunck. (pariparoba), uma planta medicinal que ocorre no Brasil, é popularmente utilizada em casos de ulcerações e irritação da pele. Dados farmacognósticos insuficientes e a grande semelhança entre as espécies de Piper levaram a realização desde trabalho, para o estabelecimento de parâmetros de analise botânica e físico-química da referida espécie. A descrição anatômica foi realizada através de lâminas permanentes e semi-permanentes do caule, folhas e raízes, corados com azul de astra e safranina. Testes histoquímicos e farmacopéicos realizados mostraram a presença de células oleíferas e determinaram os parâmetros para avaliação da qualidade e pureza. Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) Yunck. (pariparoba), a folk plant of occurrence in Brazil, is popularly used in several cases of ulceration and skin irritation. Owed to insufficient pharmacognostic data about the species and its similarity with other Piper species, the purpose of this work was to establish the botanical aspects and preliminary analysis of physical-chemical parameters. Anatomical description was undertaken by the analysis of semi-permanent and permanent slides made with different sections of leaves, stem and roots, stained with safranin and astra blue. The histochemical and pharmacopoeial tests demonstrated the presence of cells with lipophilic content and determined the parameters than can be used for quality control and purity degree. http://sedici.unlp.edu.ar:80/handle/10915/6589 Articulo Total flavonoid determination for the quality control of aqueous extractives from Phyllanthus niruri L. Acta Farmacéutica Bonaerense; vol. 22, no. 3 El objetivo de este estudio ha sido el desarrollo de una metodología de control de calidad tecnológico para la determinación de flavonoides en extractos acuosos de Phyllanthus niruri. El método de análisis se evaluó, con varias modificaciones, para determinar su adecuabilidad para la determinación cuantitativa de flavonoides totales en la materia prima vegetal y en sus extractos acuosos. El método de análisis para este grupo químico se basa en una evaluación espectrofotométrica de un complejo formado con cloruro de aluminio tras la hidrólisis ácida de los glucósidos. Los análisis estadísticos han demostrado que el método es adecuado para el análisis cuantitativo de la materia prima vegetal, pero presenta limitaciones para la evaluación de las soluciones extractivas acuosas. Dichas limitaciones pueden ser solucionadas utilizando un rango definido de cantidad de materia prima vegetal y una alícuota estándar de la solución extractiva obtenida.; The purpose of this study has been the development of an adequate methodology for the quality control of Phyllanthus niruri aqueous extracts. The assay method was tested, with various modifications, to determine its suitability for the quantitative determination of total flavonoids within the crude drug raw material and aqueous extracts. The assay technique for such chemical group is based on the spectrophotometric evaluation of a complex with AlCl after acid hydrolysis of the glycosides. Statistical analyses demonstrated the adequacy for the quantitative analysis of the raw material, but it had limitations for the assay of the aqueous extractive solutions. These limitations could be overcome by using a defined range of raw material amount and a standard aliquot of a stock extract solution. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6589 2003-01-01T00:00:00Z El objetivo de este estudio ha sido el desarrollo de una metodología de control de calidad tecnológico para la determinación de flavonoides en extractos acuosos de Phyllanthus niruri. El método de análisis se evaluó, con varias modificaciones, para determinar su adecuabilidad para la determinación cuantitativa de flavonoides totales en la materia prima vegetal y en sus extractos acuosos. El método de análisis para este grupo químico se basa en una evaluación espectrofotométrica de un complejo formado con cloruro de aluminio tras la hidrólisis ácida de los glucósidos. Los análisis estadísticos han demostrado que el método es adecuado para el análisis cuantitativo de la materia prima vegetal, pero presenta limitaciones para la evaluación de las soluciones extractivas acuosas. Dichas limitaciones pueden ser solucionadas utilizando un rango definido de cantidad de materia prima vegetal y una alícuota estándar de la solución extractiva obtenida. The purpose of this study has been the development of an adequate methodology for the quality control of Phyllanthus niruri aqueous extracts. The assay method was tested, with various modifications, to determine its suitability for the quantitative determination of total flavonoids within the crude drug raw material and aqueous extracts. The assay technique for such chemical group is based on the spectrophotometric evaluation of a complex with AlCl after acid hydrolysis of the glycosides. Statistical analyses demonstrated the adequacy for the quantitative analysis of the raw material, but it had limitations for the assay of the aqueous extractive solutions. These limitations could be overcome by using a defined range of raw material amount and a standard aliquot of a stock extract solution. http://sedici.unlp.edu.ar:80/handle/10915/6588 Articulo Anatomy and ethnobotany of two species of Boraginaceae from Pampean province (Argentina), used in popular medicine Acta Farmacéutica Bonaerense; vol. 22, no. 3 En la presente contribución se estudian dos especies de Boraginaceae utilizadas en medicina popular: Echium plantagineum L. y Heliotropium amplexicaule Valh. Para cada una de ellas se brindan: nombres científicos y vulgares, principales sinónimos, descripción, análisis histológico de las partes utilizadas, ilustración y referencias sobre etnobotánica.; In the present contribution two species of Boraginaceae used in folk medicine, Echium plantagineum L. and Heliotropium amplexicaule Valh., were studied. For each one scientific and common names, synonyms, description, histological analysis of the utilized parts, illustrations and brief ethnobotanic references are given. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6588 2003-01-01T00:00:00Z En la presente contribución se estudian dos especies de Boraginaceae utilizadas en medicina popular: Echium plantagineum L. y Heliotropium amplexicaule Valh. Para cada una de ellas se brindan: nombres científicos y vulgares, principales sinónimos, descripción, análisis histológico de las partes utilizadas, ilustración y referencias sobre etnobotánica. In the present contribution two species of Boraginaceae used in folk medicine, Echium plantagineum L. and Heliotropium amplexicaule Valh., were studied. For each one scientific and common names, synonyms, description, histological analysis of the utilized parts, illustrations and brief ethnobotanic references are given.