http://sedici.unlp.edu.ar:80/handle/10915/131 Wed, 21 Oct 2026 08:50:28 GMT 2026-10-21T08:50:28Z http://sedici.unlp.edu.ar:80/handle/10915/6612 Articulo Caracterización fisicoquímica y evaluación biológica de eritropoyetina humana recombinante en productos farmacéuticos Acta Farmacéutica Bonaerense; vol. 22, no. 4 El objetivo de este trabajo es efectuar la identificación de eritropoyetina (EPO) humana recombinante en productos farmacéuticos comerciales por electroforesis en gel de poliacrilamida y su detección con anticuerpos específicos, demostrándose típica banda larga con peso molecular entre 30 - 40 kDa. La composición de las isoformas detectadas por isoelectroenfoque (IEF) después de imunodetección y de ligación a las lectinas presentaron heterogeneidad con 5 - 8 isoformas distribuidas en la banda de puncto isoeléctrico (pI) de 4,4 - 5,2 y bandas con proporciones y posiciones significativamente diferentes entre las preparaciones. La actividad biológica fue evaluada por ensayos con ratones normocitémicos. Haciéndose una correlación entre los resultados de los bioensayos con los perfiles de IEF y su ligación a las lectinas, puede observarse que los productos con isoformas ácidas presentan mayor actividad biológica. Se evaluaran los agregados de las preparaciones sin seroalbúmina humana por cromatografía líquida a alta presión obteniendo valores entre 1,4 y 13,6 % del contenido total de EPO.; Recombinant human erythropoietin (rhEPO) from different manufacturers was analysed by non-reducing polyacrylamide gel electrophoresis and by immunodetection with a specific antiEPO antiserum, revealing a single broad diffuse band in the molecular weight range 30 - 40 kDa. The isoform compositions revealed by isoelectric focusing (IEF) after immunoblotting and lectin blotting showed extensive heterogeneity, with 5 - 8 isoforms evident over the isoelectric point (pI) range 4.4 - 5.2 and with the proportions and the position of the bands differing significantly between preparations. The biological activity of the pharmaceutical preparations of rhEPO was assessed by an in vivo bioassay using normocythaemic mice. The IEF and lectin blotting profiles generally correlated with the bioassay results, indicating that the products containing the greater proportion of acidic isoforms had the greater biopotencies. The degree of aggregation of preparations formulated without human serum albumin shown to vary between 1.4 to 13.6% of the total EPO content. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6612 2003-01-01T00:00:00Z El objetivo de este trabajo es efectuar la identificación de eritropoyetina (EPO) humana recombinante en productos farmacéuticos comerciales por electroforesis en gel de poliacrilamida y su detección con anticuerpos específicos, demostrándose típica banda larga con peso molecular entre 30 - 40 kDa. La composición de las isoformas detectadas por isoelectroenfoque (IEF) después de imunodetección y de ligación a las lectinas presentaron heterogeneidad con 5 - 8 isoformas distribuidas en la banda de puncto isoeléctrico (pI) de 4,4 - 5,2 y bandas con proporciones y posiciones significativamente diferentes entre las preparaciones. La actividad biológica fue evaluada por ensayos con ratones normocitémicos. Haciéndose una correlación entre los resultados de los bioensayos con los perfiles de IEF y su ligación a las lectinas, puede observarse que los productos con isoformas ácidas presentan mayor actividad biológica. Se evaluaran los agregados de las preparaciones sin seroalbúmina humana por cromatografía líquida a alta presión obteniendo valores entre 1,4 y 13,6 % del contenido total de EPO. Recombinant human erythropoietin (rhEPO) from different manufacturers was analysed by non-reducing polyacrylamide gel electrophoresis and by immunodetection with a specific antiEPO antiserum, revealing a single broad diffuse band in the molecular weight range 30 - 40 kDa. The isoform compositions revealed by isoelectric focusing (IEF) after immunoblotting and lectin blotting showed extensive heterogeneity, with 5 - 8 isoforms evident over the isoelectric point (pI) range 4.4 - 5.2 and with the proportions and the position of the bands differing significantly between preparations. The biological activity of the pharmaceutical preparations of rhEPO was assessed by an in vivo bioassay using normocythaemic mice. The IEF and lectin blotting profiles generally correlated with the bioassay results, indicating that the products containing the greater proportion of acidic isoforms had the greater biopotencies. The degree of aggregation of preparations formulated without human serum albumin shown to vary between 1.4 to 13.6% of the total EPO content. http://sedici.unlp.edu.ar:80/handle/10915/6611 Contribucion a revista Profile of the Buenos Aires province pharmacist Acta Farmacéutica Bonaerense; vol. 22, no. 4 El presente documento comunica los lineamientos que orientarán en el futuro mediato al proceso de capacitación-actualización profesional previsto por el Colegio de Farmacéuticos de la Provincia de Buenos Aires, enmarcado en la concepción de Educación Permanente. Responde a la necesidad de otorgar pertinencia, claridad y precisión institucional a la continuidad de acciones educativas asistidas por la Facultad de Ciencias Exactas de la Universidad Nacional de La Plata y de la Facultad de Farmacia y Bioquímica de la Universidad de Buenos Aires. Se elaboró en el Departamento de Educación y Actualización Profesional y contó con la participación de la Comisión Provincial de Educación Permanente aplicando los siguientes procedimientos: evaluación de las necesidades educativas del ejercicio profesional en el ámbito bonaerense, análisis de las tendencias internacionales en capacitación farmacéutica y determinación de los roles y actividades en términos de competencias exigidas por el desempeño profesional.; The present document communicates the outlines that will guide in the near future the professional qualification-update process foreseen by the College of Phamacists of the Province of Buenos Aires, framed in the conception of Continuous Education. It responds to the necessity of giving pertinence, clearness and institutional precision to the continuity of educative actions attended by the Faculty of Exact Sciences of the Umiversity of La Plata and the Faculty of Pharmacy and Biochemistry of the University of Buenos Aires. The report was elaborated in the Department of Education and Professional Update with the aid of the Provincial Commission of Continuous Education applying the following procedures: evaluation of the educative needs of the professional exercise in Buenos Aires Province, analysis of the international trends in pharmaceutical qualification and determination of the roles and activities in terms of competences demanded by the professional performance. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6611 2003-01-01T00:00:00Z El presente documento comunica los lineamientos que orientarán en el futuro mediato al proceso de capacitación-actualización profesional previsto por el Colegio de Farmacéuticos de la Provincia de Buenos Aires, enmarcado en la concepción de Educación Permanente. Responde a la necesidad de otorgar pertinencia, claridad y precisión institucional a la continuidad de acciones educativas asistidas por la Facultad de Ciencias Exactas de la Universidad Nacional de La Plata y de la Facultad de Farmacia y Bioquímica de la Universidad de Buenos Aires. Se elaboró en el Departamento de Educación y Actualización Profesional y contó con la participación de la Comisión Provincial de Educación Permanente aplicando los siguientes procedimientos: evaluación de las necesidades educativas del ejercicio profesional en el ámbito bonaerense, análisis de las tendencias internacionales en capacitación farmacéutica y determinación de los roles y actividades en términos de competencias exigidas por el desempeño profesional. The present document communicates the outlines that will guide in the near future the professional qualification-update process foreseen by the College of Phamacists of the Province of Buenos Aires, framed in the conception of Continuous Education. It responds to the necessity of giving pertinence, clearness and institutional precision to the continuity of educative actions attended by the Faculty of Exact Sciences of the Umiversity of La Plata and the Faculty of Pharmacy and Biochemistry of the University of Buenos Aires. The report was elaborated in the Department of Education and Professional Update with the aid of the Provincial Commission of Continuous Education applying the following procedures: evaluation of the educative needs of the professional exercise in Buenos Aires Province, analysis of the international trends in pharmaceutical qualification and determination of the roles and activities in terms of competences demanded by the professional performance. http://sedici.unlp.edu.ar:80/handle/10915/6610 Articulo Drugs of prolonged action: mechanisms of liberation Acta Farmacéutica Bonaerense; vol. 22, no. 4 El objetivo de este artÍculo es resumir y agrupar los diferentes estudios realizados hasta el presente por distintos autores, con el fin de interpretar y comprender los mecanismos de liberación controlada de los fármacos. Estos fármacos se caracterizan por poseer un núcleo (que puede ser de naturaleza inerte como los utilizados en ciertos tipos de micropartículas), que contiene la droga y el cual esta formado por una capa externa de un polímero que tiene propiedades de gelificar frente al agua o solventes biológicos a temperaturas de 37 °C. Esta matriz puede contener una capa más externa soluble o insoluble, pudiendo esta última modificar las propiedades del polímero gelificante. A los procesos de liberación de estos sistemas de matrices hinchables, se los conoce también como mecanismos de liberación anómalos, ya que la liberación del principio activo no sólo depende de la velocidad de disolución del mismo, sino también de los fenómenos de relajación que afectan a las cadenas del material polimérico.; The objetive of this paper is to summarize and group the different studies carried out until the present by different authors, with the purpose of to interpret and understand the mechanisms of controlled liberation of drugs. These drugs are characterized by possessing a nucleus (it can be of inert nature, as those used in certain multi-particulate types), that contains the active principle and which is formed by an external polymeric layer that has the property of gelling in the presence of water or biological solvents at 37 °C. This matrix so formed can contain a soluble or insoluble more external layer that can modify the properties of the gelling polymer. The liberation processes of these expanded matrices are also known as anomalous liberation mechanisms, since the liberation of the activeprinciple not only depends on its disolution speed, but also of the relaxation phenomena that affect the chains of the polymeric materials. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6610 2003-01-01T00:00:00Z El objetivo de este artÍculo es resumir y agrupar los diferentes estudios realizados hasta el presente por distintos autores, con el fin de interpretar y comprender los mecanismos de liberación controlada de los fármacos. Estos fármacos se caracterizan por poseer un núcleo (que puede ser de naturaleza inerte como los utilizados en ciertos tipos de micropartículas), que contiene la droga y el cual esta formado por una capa externa de un polímero que tiene propiedades de gelificar frente al agua o solventes biológicos a temperaturas de 37 °C. Esta matriz puede contener una capa más externa soluble o insoluble, pudiendo esta última modificar las propiedades del polímero gelificante. A los procesos de liberación de estos sistemas de matrices hinchables, se los conoce también como mecanismos de liberación anómalos, ya que la liberación del principio activo no sólo depende de la velocidad de disolución del mismo, sino también de los fenómenos de relajación que afectan a las cadenas del material polimérico. The objetive of this paper is to summarize and group the different studies carried out until the present by different authors, with the purpose of to interpret and understand the mechanisms of controlled liberation of drugs. These drugs are characterized by possessing a nucleus (it can be of inert nature, as those used in certain multi-particulate types), that contains the active principle and which is formed by an external polymeric layer that has the property of gelling in the presence of water or biological solvents at 37 °C. This matrix so formed can contain a soluble or insoluble more external layer that can modify the properties of the gelling polymer. The liberation processes of these expanded matrices are also known as anomalous liberation mechanisms, since the liberation of the activeprinciple not only depends on its disolution speed, but also of the relaxation phenomena that affect the chains of the polymeric materials. http://sedici.unlp.edu.ar:80/handle/10915/6609 Articulo Pharmaceutical care in psychiatric outpatients Acta Farmacéutica Bonaerense; vol. 22, no. 4 El presente artículo aborda de una manera introductoria la evolución desde la Farmacia Clínica hasta la Atención Farmacéutica y su aplicación en el ámbito de la Salud Mental. Se explica el papel que el farmacéutico de oficina puede realizar a fin de mejorar la salud y calidad de vida de los pacientes con trastornos psiquiátricos en el ámbito ambulatorio.; This article broaches in an introductory way the evolution from the Clinical Pharmacy to the Pharmaceutical Care and their application in the mental health. It is explained the rol that the pharmacist in a community pharmacy can do with the purpose of the improvement of health and quality of life of psychiatric outpatients. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6609 2003-01-01T00:00:00Z El presente artículo aborda de una manera introductoria la evolución desde la Farmacia Clínica hasta la Atención Farmacéutica y su aplicación en el ámbito de la Salud Mental. Se explica el papel que el farmacéutico de oficina puede realizar a fin de mejorar la salud y calidad de vida de los pacientes con trastornos psiquiátricos en el ámbito ambulatorio. This article broaches in an introductory way the evolution from the Clinical Pharmacy to the Pharmaceutical Care and their application in the mental health. It is explained the rol that the pharmacist in a community pharmacy can do with the purpose of the improvement of health and quality of life of psychiatric outpatients. http://sedici.unlp.edu.ar:80/handle/10915/6608 Articulo Acta Farmacéutica Bonaerense; vol. 22, no. 4 Se describe un método rápido, simple, económico y preciso para la determinación de la fluoroquinona gatifloxacina en comprimidos. El procedimiento está basado en el uso de dosaje volumétrico en un medio no acuoso de ácido acético glacial con ácido perclórico 0,1 M. La validación del método produjo buenos resultados que incluyen precisión, recuperación y seguridad. Se encontró además que los excipientes del comprimido no interfieren en el ensayo.; An inexpensive, simple, precise and rapid method for the determination of fluoroquinolone gatifloxacin in tablets is described. The procedure is based on the use of volumetric dosage in a non-aqueous medium in glacial acetic acid with 0.1 M perchloric acid. The method validation yielded good results and included the precision, recovery and accuracy. It was also found that the excipients in the commercial tablet preparation did not interfere with the assay. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6608 2003-01-01T00:00:00Z Se describe un método rápido, simple, económico y preciso para la determinación de la fluoroquinona gatifloxacina en comprimidos. El procedimiento está basado en el uso de dosaje volumétrico en un medio no acuoso de ácido acético glacial con ácido perclórico 0,1 M. La validación del método produjo buenos resultados que incluyen precisión, recuperación y seguridad. Se encontró además que los excipientes del comprimido no interfieren en el ensayo. An inexpensive, simple, precise and rapid method for the determination of fluoroquinolone gatifloxacin in tablets is described. The procedure is based on the use of volumetric dosage in a non-aqueous medium in glacial acetic acid with 0.1 M perchloric acid. The method validation yielded good results and included the precision, recovery and accuracy. It was also found that the excipients in the commercial tablet preparation did not interfere with the assay. http://sedici.unlp.edu.ar:80/handle/10915/6607 Articulo Stability studies of a cream made from a dry extract of the bark of Mangifera indica L. (Vimang) Acta Farmacéutica Bonaerense; vol. 22, no. 4 La estabilidad de la crema VIMANG, elaborada a partir de un extracto seco de la corteza de Mangifera indica L., fue estudiada en tres condiciones de almacenamiento (acelerado, temperatura de estante y en refrigeración) por dos años. Se evaluaron la extensibilidad, pH, conteo microbiano y el porciento de los fenoles totales, mediante el cual se determinó la estabilidad química. Los resultados del estudio demostraron que la crema almacenada en condiciones de refrigeración (2-8 °C) es estable durante 18 meses. A temperatura ambiente es estable durante 3 meses mientras que a 45(C no es estable.; The results of a stability study of a cream produced from a dry extract of the bark of Mangifera indica L. at accelerated, normal and refrigerated conditions for two years, is reported. Organoleptic characteristics, total phenols content, pH, extensibility and a microbial count were carried out. It was demonstrated that the VIMANG cream stored at 45 ºC is very unstable, under normal conditions is stable up to 3 months, while under refrigerated conditions it is stable up to 18 months. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6607 2003-01-01T00:00:00Z La estabilidad de la crema VIMANG, elaborada a partir de un extracto seco de la corteza de Mangifera indica L., fue estudiada en tres condiciones de almacenamiento (acelerado, temperatura de estante y en refrigeración) por dos años. Se evaluaron la extensibilidad, pH, conteo microbiano y el porciento de los fenoles totales, mediante el cual se determinó la estabilidad química. Los resultados del estudio demostraron que la crema almacenada en condiciones de refrigeración (2-8 °C) es estable durante 18 meses. A temperatura ambiente es estable durante 3 meses mientras que a 45(C no es estable. The results of a stability study of a cream produced from a dry extract of the bark of Mangifera indica L. at accelerated, normal and refrigerated conditions for two years, is reported. Organoleptic characteristics, total phenols content, pH, extensibility and a microbial count were carried out. It was demonstrated that the VIMANG cream stored at 45 ºC is very unstable, under normal conditions is stable up to 3 months, while under refrigerated conditions it is stable up to 18 months. http://sedici.unlp.edu.ar:80/handle/10915/6606 Articulo Spray drying parameters optimization for chitosan microparticles as insulin carrier Acta Farmacéutica Bonaerense; vol. 22, no. 4 O objetivo deste trabalho foi determinar a influência dos parâmetros de nebulização no rendimento, granulometria e morfologia das micropartículas de quitosana como potencial carreadores da insulina. Quitosana a 1% e 2% (m/v) foi nebulizada em diferentes condições de fluxo e temperatura de entrada. A granulometria foi estudada por microscopia óptica e a morfologia por MEV. A concentração de quitosana foi o fator que mais influenciou no rendimento. A forma esférica foi predominante, com distribuição granulométrica compatível para permanência no septo nasal. As soluções poliméricas mais concentradas e a inclusão de insulina levaram a um aumento no diâmetro das partículas e a uma superfície menos rugosa.; This study aimed to determine the influence of spray drying parameters on yield, particle size distribution and morphology of chitosan microparticles with potential as insulin carrier. Chitosan 1% and 2% (w/v) solutions were spray dried under different flow rates and inlet temperatures. Particle size distribution was determined by optical microscopy and surface morphology was examined by SEM. Chitosan concentration was the major factor that influenced the yields. Spherical particles predominated and their granulometry distribution showed to be compatible with permanence in the nasal septum. Solutions with higher polymer content as well the addition of insulin led to an increase in particle size and a smoother surface. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6606 2003-01-01T00:00:00Z O objetivo deste trabalho foi determinar a influência dos parâmetros de nebulização no rendimento, granulometria e morfologia das micropartículas de quitosana como potencial carreadores da insulina. Quitosana a 1% e 2% (m/v) foi nebulizada em diferentes condições de fluxo e temperatura de entrada. A granulometria foi estudada por microscopia óptica e a morfologia por MEV. A concentração de quitosana foi o fator que mais influenciou no rendimento. A forma esférica foi predominante, com distribuição granulométrica compatível para permanência no septo nasal. As soluções poliméricas mais concentradas e a inclusão de insulina levaram a um aumento no diâmetro das partículas e a uma superfície menos rugosa. This study aimed to determine the influence of spray drying parameters on yield, particle size distribution and morphology of chitosan microparticles with potential as insulin carrier. Chitosan 1% and 2% (w/v) solutions were spray dried under different flow rates and inlet temperatures. Particle size distribution was determined by optical microscopy and surface morphology was examined by SEM. Chitosan concentration was the major factor that influenced the yields. Spherical particles predominated and their granulometry distribution showed to be compatible with permanence in the nasal septum. Solutions with higher polymer content as well the addition of insulin led to an increase in particle size and a smoother surface. http://sedici.unlp.edu.ar:80/handle/10915/6605 Articulo Postnatal development of offsprings exposed to hydro alcoholic extract of Cissampelos sympodialis Eichl., during the pregnancy Acta Farmacéutica Bonaerense; vol. 22, no. 4 Neste trabalho foram investigados os possíveis efeitos tóxicos no desenvolvimento da prole, decorrentes do tratamento de ratas prenhes com o extrato hidroalcoólico das folhas da Cissampelos sympodialis Eichl. Foram registrados: duração da gestação; ganho de peso da mãe; número de filhotes por ninhada; peso dos filhotes; reflexo de endireitamento; dia de abertura dos olhos; dia de andar adulto e ocorrência de malformações. Os resultados sugerem a ausência de efeitos adversos desta planta durante a gestação. Testes complementares deverão ser realizados, visando dar maior suporte à realização dos estudos clínicos.; In the present study, possible toxic effects on development of the offsprings were investigated after the treatment of pregnant female rats with the hydroalcoholic extract of Cissampelos sympodialis Eichl. leaves. The following parameters were analyzed: pregnancy duration; weight gain; litter size; body weight of the pups; righting reflex; eye opening; hind paws supporting and body lifting; external malformations occurrence. The results suggest the absence of side effects of this plant during pregnancy. Other tests should be carried out to give further support to clinical studies. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6605 2003-01-01T00:00:00Z Neste trabalho foram investigados os possíveis efeitos tóxicos no desenvolvimento da prole, decorrentes do tratamento de ratas prenhes com o extrato hidroalcoólico das folhas da Cissampelos sympodialis Eichl. Foram registrados: duração da gestação; ganho de peso da mãe; número de filhotes por ninhada; peso dos filhotes; reflexo de endireitamento; dia de abertura dos olhos; dia de andar adulto e ocorrência de malformações. Os resultados sugerem a ausência de efeitos adversos desta planta durante a gestação. Testes complementares deverão ser realizados, visando dar maior suporte à realização dos estudos clínicos. In the present study, possible toxic effects on development of the offsprings were investigated after the treatment of pregnant female rats with the hydroalcoholic extract of Cissampelos sympodialis Eichl. leaves. The following parameters were analyzed: pregnancy duration; weight gain; litter size; body weight of the pups; righting reflex; eye opening; hind paws supporting and body lifting; external malformations occurrence. The results suggest the absence of side effects of this plant during pregnancy. Other tests should be carried out to give further support to clinical studies. http://sedici.unlp.edu.ar:80/handle/10915/6604 Articulo Morpho-anatomical characters of Baccharis gaudichaudiana DC., Asteraceae Acta Farmacéutica Bonaerense; vol. 22, no. 4 Baccharis gaudichaudiana DC., Asteraceae, conhecida popularmente como carqueja e chilca melosa, é empregada na medicina tradicional como estomáquico, diurético e agente hipoglicêmico. Objetivou- se estudar a morfologia externa e a anatomia dos caules alados, a fim de fornecer informações que auxiliem na identificação farmacognóstica dessa planta medicinal e contribuam com a taxonomia do grupo Trimera. O material botânico foi submetido às microtécnicas fotônicas e eletrônicas de varredura usuais. A espécie mede cerca de 70 cm de altura e apresenta caule trialado. A epiderme é uniestratificada e revestida por cutícula levemente estriada, e possui estômatos anomocíticos e tricomas glandulares pluricelulares. Nas alas, o clorênquima se diferencia em parênquima paliçádico em posição adjacente às duas faces da epiderme e em parênquima lacunoso na região mediana. No eixo caulinar, o clorênquima se alterna com o colênquima angular, o cilindro vascular consiste de floema em posição periférica e xilema formado centripetamente, e na zona perimedular são encontrados cristais prismáticos de oxalato de cálcio. Dutos secretores, com epitélio unisseriado, são observados nas alas e no eixo caulinar.; Baccharis gaudichaudiana DC., Asteraceae, popularly known as carqueja and chilca melosa, is employed in the traditional medicine as stomachic, diuretic and hypoglycemic agent. The aim of this work was to study the winged stem morpho-anatomy, in order to supply information to the pharmacognostical identification of this medicinal plant and to the taxonomy of the Trimera group. The botanical material was prepared according to the usual optical and electronic microtechniques. The species is 70 cm height and has got a three-winged stem. The epidermis is uniseriate and coated by a slightly striated cuticle, and it has got anomocytic stomata and pluricellular glandular trichomes. In the caulinar expansions, the clorenchyma consists of palisade parenchyma on both sides of the epidermis and spongy parenchyma in the middle. In the caulinar axis, the clorenchyma and the angular collenchyma distribute alternately, the vascular cylinder is formed with phloem, centrifugally and xylem, centripetally, and in the perimedular zone prismatic crystals are found. Secretory ducts, with uniseriate epithelium, are observed in the stem. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6604 2003-01-01T00:00:00Z Baccharis gaudichaudiana DC., Asteraceae, conhecida popularmente como carqueja e chilca melosa, é empregada na medicina tradicional como estomáquico, diurético e agente hipoglicêmico. Objetivou- se estudar a morfologia externa e a anatomia dos caules alados, a fim de fornecer informações que auxiliem na identificação farmacognóstica dessa planta medicinal e contribuam com a taxonomia do grupo Trimera. O material botânico foi submetido às microtécnicas fotônicas e eletrônicas de varredura usuais. A espécie mede cerca de 70 cm de altura e apresenta caule trialado. A epiderme é uniestratificada e revestida por cutícula levemente estriada, e possui estômatos anomocíticos e tricomas glandulares pluricelulares. Nas alas, o clorênquima se diferencia em parênquima paliçádico em posição adjacente às duas faces da epiderme e em parênquima lacunoso na região mediana. No eixo caulinar, o clorênquima se alterna com o colênquima angular, o cilindro vascular consiste de floema em posição periférica e xilema formado centripetamente, e na zona perimedular são encontrados cristais prismáticos de oxalato de cálcio. Dutos secretores, com epitélio unisseriado, são observados nas alas e no eixo caulinar. Baccharis gaudichaudiana DC., Asteraceae, popularly known as carqueja and chilca melosa, is employed in the traditional medicine as stomachic, diuretic and hypoglycemic agent. The aim of this work was to study the winged stem morpho-anatomy, in order to supply information to the pharmacognostical identification of this medicinal plant and to the taxonomy of the Trimera group. The botanical material was prepared according to the usual optical and electronic microtechniques. The species is 70 cm height and has got a three-winged stem. The epidermis is uniseriate and coated by a slightly striated cuticle, and it has got anomocytic stomata and pluricellular glandular trichomes. In the caulinar expansions, the clorenchyma consists of palisade parenchyma on both sides of the epidermis and spongy parenchyma in the middle. In the caulinar axis, the clorenchyma and the angular collenchyma distribute alternately, the vascular cylinder is formed with phloem, centrifugally and xylem, centripetally, and in the perimedular zone prismatic crystals are found. Secretory ducts, with uniseriate epithelium, are observed in the stem. http://sedici.unlp.edu.ar:80/handle/10915/6603 Articulo Vehiculization of anthralin into n-alkyl ascorbic acid derivatives coagels Acta Farmacéutica Bonaerense; vol. 22, no. 4 Los tratamientos antipsoriásicos utilizando antralina presentan varias desventajas debido principalmente a las propiedades físico-químicas de ésta, a sus efectos adversos y a su formulación. La antralina es insoluble en agua, químicamente inestable y produce ardor y tinción en la zona de aplicación y vestimentas. En el presente trabajo se evalúa la potencial vehiculización de antralina en agregados supramoleculares de derivados n-alquílicos del ácido ascórbico (ASCn) con el objeto de obtener nuevas formas farmacéuticas semisólidas (FFsS). Estos sistemas presentan un gran potencial de solubilización de drogas y la presencia del grupo ascorbilo provee un efecto estabilizante para drogas con problemas de estabilidad en medio acuoso. Se estudiaron propiedades como solubilización de la droga en las dispersiones coloidales, determinación de la capacidad de carga en los coageles, efecto del agregado de co-solventes sobre estas propiedades, estabilidad de antralina en los coageles y sus propiedades reológicas. La solubilidad aparente de antralina fue significativamente incrementada en los agregados supramoleculares de ASCn. El agregado de co-solventes como PEG aumentó varias veces la capacidad de carga de antralina en coageles de ASC16. Asimismo, la estabilidad de la droga fue dramáticamente incrementada en estos sistemas en comparación con soluciones etanólicas de antralina.; Anthralin, vehiculized in semisolids pharmaceutical dosage forms, is used in pharmacological treatment of psoriasis. The physicochemical properties and the drug side effect difficult the design and utilization of suitables formulations. Anthralin has very low aqueous solubility, it is unstable and its efficacy remains hampered by the effects of irritation and staining of the perilesional skin. In this work, the potential vehiculization of anthralin into supramolecular aggregates of n-alkyl ascorbic acid derivatives (ASCn) was evaluated. These compounds can form supramolecular aggregates above critical micelar temperature (TMC) and liquids crystal structures (coagels) as temperature decrease below TMC. This kind of systems showed high potential for drug solubilization and besides the ascorbyl moiety can contribute to stabilize the drug in the aggregate. Anthralin solubilization in ASCn colloidal dispersions and coagels, the effect of cosolvents on solubilization, drug stability in the coagels and its rheological properties were studied. The anthralin apparent solubility was significantly increased. The incorporation of PEG augmented several times the solubilization capacity of ASC16 coagels. Likewise, Anthralin stability was dramatically raised in these systems comparatively to ethanolic solutions of the drug. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6603 2003-01-01T00:00:00Z Los tratamientos antipsoriásicos utilizando antralina presentan varias desventajas debido principalmente a las propiedades físico-químicas de ésta, a sus efectos adversos y a su formulación. La antralina es insoluble en agua, químicamente inestable y produce ardor y tinción en la zona de aplicación y vestimentas. En el presente trabajo se evalúa la potencial vehiculización de antralina en agregados supramoleculares de derivados n-alquílicos del ácido ascórbico (ASCn) con el objeto de obtener nuevas formas farmacéuticas semisólidas (FFsS). Estos sistemas presentan un gran potencial de solubilización de drogas y la presencia del grupo ascorbilo provee un efecto estabilizante para drogas con problemas de estabilidad en medio acuoso. Se estudiaron propiedades como solubilización de la droga en las dispersiones coloidales, determinación de la capacidad de carga en los coageles, efecto del agregado de co-solventes sobre estas propiedades, estabilidad de antralina en los coageles y sus propiedades reológicas. La solubilidad aparente de antralina fue significativamente incrementada en los agregados supramoleculares de ASCn. El agregado de co-solventes como PEG aumentó varias veces la capacidad de carga de antralina en coageles de ASC16. Asimismo, la estabilidad de la droga fue dramáticamente incrementada en estos sistemas en comparación con soluciones etanólicas de antralina. Anthralin, vehiculized in semisolids pharmaceutical dosage forms, is used in pharmacological treatment of psoriasis. The physicochemical properties and the drug side effect difficult the design and utilization of suitables formulations. Anthralin has very low aqueous solubility, it is unstable and its efficacy remains hampered by the effects of irritation and staining of the perilesional skin. In this work, the potential vehiculization of anthralin into supramolecular aggregates of n-alkyl ascorbic acid derivatives (ASCn) was evaluated. These compounds can form supramolecular aggregates above critical micelar temperature (TMC) and liquids crystal structures (coagels) as temperature decrease below TMC. This kind of systems showed high potential for drug solubilization and besides the ascorbyl moiety can contribute to stabilize the drug in the aggregate. Anthralin solubilization in ASCn colloidal dispersions and coagels, the effect of cosolvents on solubilization, drug stability in the coagels and its rheological properties were studied. The anthralin apparent solubility was significantly increased. The incorporation of PEG augmented several times the solubilization capacity of ASC16 coagels. Likewise, Anthralin stability was dramatically raised in these systems comparatively to ethanolic solutions of the drug. http://sedici.unlp.edu.ar:80/handle/10915/6602 Articulo Anatomy of the vegetative organs of Vernonia Brasiliana (L.) Druce Acta Farmacéutica Bonaerense; vol. 22, no. 4 Descrições anatômicas de raiz, caule e folha de Vernonia brasiliana (L.) Druce foram realizadasem cortes transversais e paradérmicos, à mão livre, corados com safranina/azul de astra. Todas as análises foram feitas em imagens digitais sob microscópio ótico pré-calibrado. A espécie apresentou tricomas não-glandulares em forma da letra T, distribuídos por toda a planta, principalmente na epiderme inferior das folhas. Alguns tricomas capitados foram observados na epiderme inferior das folhas. O caule apresentou estrutura do tipo eustele com feixes vasculares colaterais de tamanhos variados. Na raiz, os feixes vasculares colaterais estavam circundados por uma faixa descontínua de fibras agrupadas. A região medular apresentou células com as menores dimensões na posição central.; Anatomical descriptions of root, stem and leaf of Vernonia brasiliana (L.) Druce were made. Transversal and paradermal free-hand sections of fresh material were stained with safranin/astra blue. The analysis was done in digital images under a pre-calibrated light microscope. Non-glandular two-arms T-shaped trichomes were found in the whole plant, mainly in the lower epidermis of the leaves. Some capitate trichomes in the lower epidermis of the leaves were observed. The stem showed collateral vascular bundles of different sizes. In root, the vascular bundles were collateral with a discontinuous band of clustered fiber cells. The pith region showed smaller parenchyma cells in the central position. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6602 2003-01-01T00:00:00Z Descrições anatômicas de raiz, caule e folha de Vernonia brasiliana (L.) Druce foram realizadasem cortes transversais e paradérmicos, à mão livre, corados com safranina/azul de astra. Todas as análises foram feitas em imagens digitais sob microscópio ótico pré-calibrado. A espécie apresentou tricomas não-glandulares em forma da letra T, distribuídos por toda a planta, principalmente na epiderme inferior das folhas. Alguns tricomas capitados foram observados na epiderme inferior das folhas. O caule apresentou estrutura do tipo eustele com feixes vasculares colaterais de tamanhos variados. Na raiz, os feixes vasculares colaterais estavam circundados por uma faixa descontínua de fibras agrupadas. A região medular apresentou células com as menores dimensões na posição central. Anatomical descriptions of root, stem and leaf of Vernonia brasiliana (L.) Druce were made. Transversal and paradermal free-hand sections of fresh material were stained with safranin/astra blue. The analysis was done in digital images under a pre-calibrated light microscope. Non-glandular two-arms T-shaped trichomes were found in the whole plant, mainly in the lower epidermis of the leaves. Some capitate trichomes in the lower epidermis of the leaves were observed. The stem showed collateral vascular bundles of different sizes. In root, the vascular bundles were collateral with a discontinuous band of clustered fiber cells. The pith region showed smaller parenchyma cells in the central position. http://sedici.unlp.edu.ar:80/handle/10915/6601 Articulo Determination of the incidence level of vascular or thrombotic recidiving on anticoagulated patients treated with acenocumarol Acta Farmacéutica Bonaerense; vol. 22, no. 4 La enfermedad aterotrombótica es la primera causa de morbimortalidad en el mundo occidental, razón por la cual la necesidad de una terapia anticoagulante es cada día más frecuente. Las patologías que la requieren son generadas por una activación no deseada de la coagulación, que condiciona la formación de trombos dentro del circuito vascular. El objetivo del presente trabajo fue analizar los incidentes sufridos por un grupo de pacientes, seleccionados aleatoriamente, que habían recibido tratamiento de anticoagulación oral con acenocumarol para la prevención secundaria de accidentes cerebro vasculares, aplicando el método desarrollado por Rosendaal et. al. 1 , que permitió estimar el nivel de incidencia de los niveles de anticoagulación y los beneficios y riesgos de los tratamientos. Durante un tiempo promedio de observación de 28,3 meses, en 228 pacientes incluídos en el estudio y con un total de 249,81 pacientes/año, se registraron 42 episodios hemorrágicos (EH) con una incidencia del 9,8% pacientes/año; en 33 de ellos se obtuvieron los valores de la Razón Internacional Normatizada (RIN) antes del episodio, de los cuales 6 fueron episodios mayores (1,4% paciente/año) y sólo 2 fueron causal de muerte (0,5% pa-cientes/año). Por otro lado se registraron 10 episodios trombóticos (ET) (2,3% pacientes/año) de los que en 3 casos se obtuvieron valores de RIN y dos de ellos terminaron con el fallecimiento del paciente (0,5% pa-cientes/año). El estudio permitió demostrar que el empleo de acenocumarol como anticoagulante oral brinda una terapia segura y eficaz en el corto, mediano y largo plazo.; Atherothrombosis is the first morbimortality cause in Occident; therefore, the necessity of anticoagulant therapy is more frequent every day. The pathologies that require this therapy are generated by a not wanted activation of the clotting process, that promotes thrombus formation inside the vascular circuit. The aim of the present work was to analyze the incidents suffered by a group of patients, randomly selected, that received oral anticoagulation treatment with acenocumarol for the secondary prevention of vascular accidents, using the method developed by Rosendaal et al. 1 , that allows the determination of the incidence levels of clotting and the benefits and risks of the treatments. During an average time of 28,3 months, within a total of 249,81 patients/year, in 228 patients included in the study 42 hemorragic events (EH) were registered, with an incidence of 9.8% patients/year; in 33 cases the International Normalized Ratio (INR) values were obtained before the episode, of which 6 were major episodes (1.4% patients/year) and only 2 causal of death (0.5% patients/year). On the other hand 10 thrombotic events (ET) were registered (4,4% patients/year), of which in 3 cases the INR values were obtained (2,3% patients/year) and two of them finished with the patients death (0,5% patients/year). The study demonstrated that the use of acenocumarol as oral anticoagulant offers a sure and effective therapy in short, medium and large time. Wed, 01 Jan 2003 00:00:00 GMT http://sedici.unlp.edu.ar:80/handle/10915/6601 2003-01-01T00:00:00Z La enfermedad aterotrombótica es la primera causa de morbimortalidad en el mundo occidental, razón por la cual la necesidad de una terapia anticoagulante es cada día más frecuente. Las patologías que la requieren son generadas por una activación no deseada de la coagulación, que condiciona la formación de trombos dentro del circuito vascular. El objetivo del presente trabajo fue analizar los incidentes sufridos por un grupo de pacientes, seleccionados aleatoriamente, que habían recibido tratamiento de anticoagulación oral con acenocumarol para la prevención secundaria de accidentes cerebro vasculares, aplicando el método desarrollado por Rosendaal et. al. 1 , que permitió estimar el nivel de incidencia de los niveles de anticoagulación y los beneficios y riesgos de los tratamientos. Durante un tiempo promedio de observación de 28,3 meses, en 228 pacientes incluídos en el estudio y con un total de 249,81 pacientes/año, se registraron 42 episodios hemorrágicos (EH) con una incidencia del 9,8% pacientes/año; en 33 de ellos se obtuvieron los valores de la Razón Internacional Normatizada (RIN) antes del episodio, de los cuales 6 fueron episodios mayores (1,4% paciente/año) y sólo 2 fueron causal de muerte (0,5% pa-cientes/año). Por otro lado se registraron 10 episodios trombóticos (ET) (2,3% pacientes/año) de los que en 3 casos se obtuvieron valores de RIN y dos de ellos terminaron con el fallecimiento del paciente (0,5% pa-cientes/año). El estudio permitió demostrar que el empleo de acenocumarol como anticoagulante oral brinda una terapia segura y eficaz en el corto, mediano y largo plazo. Atherothrombosis is the first morbimortality cause in Occident; therefore, the necessity of anticoagulant therapy is more frequent every day. The pathologies that require this therapy are generated by a not wanted activation of the clotting process, that promotes thrombus formation inside the vascular circuit. The aim of the present work was to analyze the incidents suffered by a group of patients, randomly selected, that received oral anticoagulation treatment with acenocumarol for the secondary prevention of vascular accidents, using the method developed by Rosendaal et al. 1 , that allows the determination of the incidence levels of clotting and the benefits and risks of the treatments. During an average time of 28,3 months, within a total of 249,81 patients/year, in 228 patients included in the study 42 hemorragic events (EH) were registered, with an incidence of 9.8% patients/year; in 33 cases the International Normalized Ratio (INR) values were obtained before the episode, of which 6 were major episodes (1.4% patients/year) and only 2 causal of death (0.5% patients/year). On the other hand 10 thrombotic events (ET) were registered (4,4% patients/year), of which in 3 cases the INR values were obtained (2,3% patients/year) and two of them finished with the patients death (0,5% patients/year). The study demonstrated that the use of acenocumarol as oral anticoagulant offers a sure and effective therapy in short, medium and large time.