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dc.date.accessioned 2012-04-04T14:35:02Z
dc.date.available 2012-04-04T03:00:00Z
dc.date.issued 2012 es
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/8447
dc.description.abstract A sensitive and selective liquid chromatography-mass spectrometry (LC–MS) method for determination of esomeprazole in rabbit plasma was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation, and chromatography involved Agilent SB-C18 column (2.1 x 150 mm, 5.0 μm) using 0.1 % formic acid in water and acetonitrile as a mobile phase with gradient elution. Detection involved positive ion mode electrospray ionization (ESI), and selective ion monitoring (SIM) mode was used for quantification of target fragment ions m/z 198 for esomeprazole and m/z 326 for midazolam (internal standard, IS). The assay was linear over the range of 10–2000 ng/mL for esomeprazole, with a lower limit of quantitation (LLOQ) of 10 ng/mL for esomeprazole. Intra- and inter-day precisions were less than 14 % and the accuracies were in the range of 89.2-112.6 % for esomeprazole. This developed method was successfully applied for the determination of esomeprazole in rabbit plasma for pharmacokinetic study. es
dc.format.extent 167-171 es
dc.language en es
dc.subject Inhibidores de la Bomba de Protones es
dc.subject Espectrometría de Masas es
dc.subject Cromatografía Liquida es
dc.subject Plasma es
dc.subject Conejos es
dc.subject Farmacocinética es
dc.title Determination of esomeprazole in rabbit plasma by liquid chromatography-mass spectrometry and its application to a pharmacokinetic study es
dc.type Articulo es
sedici.identifier.uri http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_2_6.pdf es
sedici.creator.person Zhang, Meiling es
sedici.creator.person Wang, Xuebao es
sedici.creator.person Wang, Zhiyi es
sedici.creator.person Deng, Mingjie es
sedici.creator.person Xu, Zhisheng es
sedici.creator.person Zheng, Yuancai es
sedici.creator.person Zhang, Yuan es
sedici.creator.person Huang, Xueli es
sedici.subject.materias Farmacia es
sedici.description.fulltext false es
mods.originInfo.place Colegio de Farmacéuticos de la Provincia de Buenos Aires es
sedici.subtype Comunicacion es
sedici.description.peerReview peer-review es
sedici2003.identifier ARG-FARM-ART-0000001935 es
sedici.relation.journalTitle Latin American Journal of Pharmacy es
sedici.relation.journalVolumeAndIssue vol. 31, no. 1 es


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