Δ9 desaturase activity in normal mouse liver and hepatoma SS1K

Abstract

The activity of Δ9 desaturase was determined in the microsomal fraction of normal mouse liver and hepatoma SS1K in the presence of the 105,000 × g supernatant. Neither hepatic nor hepatoma soluble fractions were able to modify the low desaturating capacity. Two enzymes from the microsomal electron transport chain associated with Δ9 desaturase, namely NADH-cytochrom b5 reductase and NADH-cytochrome C reductase were also measured. The results indicate that the low Δ9 desaturase activity in hepatoma SS1K could be related to the reduced amount of desaturase.