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dc.date.accessioned 2020-08-13T18:18:12Z
dc.date.available 2020-08-13T18:18:12Z
dc.date.issued 2013-09
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/102270
dc.description.abstract Integrins are heterodimeric cell-surface adhesion receptors that play a critical role in tissue development. Characterization of the full-length mRNA encoding the β1 subunit (Itgb1) revealed an alternative functional cleavage and polyadenylation site that yields a new Itgb1 mRNA isoform 578 bp shorter than that previously reported. Using a variety of experimental and bioinformatic approaches, we found that the two Itgb1 isoforms are expressed at different levels in a variety of mouse tissues, including the mammary gland, where they are differentially regulated at successive developmental stages. The longer mRNA species is prevelant during lactation, whereas the shorter is induced after weaning. In 3D cultures, where expression of integrin β1 protein is required for normal formation of acini, experimental blockade of the longer isoform induced enhanced expression of the shorter species which allowed normal morphological mammary differentiation. The short isoform lacks AU-rich motifs and miRNA target sequences that are potentially implicated in the regulation of mRNA stability and translation efficiency. We further determined that the AU-binding protein HuR appears to selectively stabilize the longer isoform in the mammary gland. In summary, the results of the present study identify a new regulatory instance involved in the fine-tuning of Itgb1 expression during mammary gland development and function. en
dc.format.extent 345-357 es
dc.language en es
dc.subject Alternative polyadenylation es
dc.subject Gene expression regulation es
dc.subject HuR es
dc.subject Itgbl es
dc.subject Mammary gland es
dc.subject mRNA es
dc.title The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development en
dc.type Articulo es
sedici.identifier.uri https://ri.conicet.gov.ar/11336/13692 es
sedici.identifier.uri http://www.biochemj.org/content/454/2/345 es
sedici.identifier.other https://doi.org/10.1042/BJ20130062 es
sedici.identifier.other hdl:11336/13692 es
sedici.identifier.issn 1470-8728 es
sedici.creator.person Naipauer, Julián es
sedici.creator.person Gatelli, Albana es
sedici.creator.person Degese, María Sol es
sedici.creator.person Slomiansky, Victoria es
sedici.creator.person Wertheimer Hermitte, Eva Victoria es
sedici.creator.person Lamarre, Jonathan es
sedici.creator.person Castilla, Lucio es
sedici.creator.person Abba, Martín Carlos es
sedici.creator.person Kordon, Edith Claudia es
sedici.creator.person Coso, Omar Adrian es
sedici.subject.materias Bioquímica es
sedici.description.fulltext true es
mods.originInfo.place Centro de Investigaciones Inmunológicas Básicas y Aplicadas es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Biochemical Journal es
sedici.relation.journalVolumeAndIssue vol. 454, no. 2 es

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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Except where otherwise noted, this item's license is described as Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)