Upload resources

Upload your works to SEDICI to increase its visibility and improve its impact


Show simple item record

dc.date.accessioned 2009-06-09T12:06:01Z
dc.date.available 2009-06-09T03:00:00Z
dc.date.issued 2004-11
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/10287
dc.description.abstract In previous studies have been reported a controversy between the reactivity of mono and polyclonal antibodies in different cells populations of the mouse. In this study we focus on the immunoreactivity of the monoclonal antibody VEGF (C-1) in order to analyze its expression in two cell populations of regenerating mouse liver (hepatocytes and endothelial cells) after partial hepatectomy, and two transplanted hepatocarcinomas (ES2 and SS1K). C3H/S male mice were divided into two groups and kept under standard conditions for circadian periodicity analysis, one group were transplanted with the tumors into the subcutaneous tissue of the animal flank and the other group were subjected to a partial hepatectomy (70 %). The animals were killed by decapitation and exsanguination. Samples of regeneration liver, ES2 and SS1K tumors were processed for histology and immunostained with VEGF (C-1) mouse monoclonal IgG2a antibody. The results showed strongly immunopositivity reaction for VEGF either in hepatocytes or endothelial cells of mouse regenerating liver. We also demonstrated positive immunoreaction in both malignant tumors ES2 and SS1K endothelial and parenchymal cells. We conclude that the use of VEGF (C-1) mouse monoclonal IgG2a antibody can be applied successfully in experimental design leading to study processes involved in mice’s angiogenesis en
dc.format.extent 13-18 es
dc.language en es
dc.subject mouse; regeneration liver; hepatocarcinoma; VEGF en
dc.subject experimentación animal es
dc.subject sustancia cancerígena es
dc.title Expression of vascular endothelial growth factor in normal and tumoral liver mouse cells en
dc.type Articulo es
sedici.identifier.uri http://revista.med.unlp.edu.ar/archivos/200411/expresion%20vascular.pdf es
sedici.creator.person Errecalde, Ana Lía es
sedici.creator.person Inda, Ana María es
sedici.creator.person García, Marcela Nilda es
sedici.creator.person Andrini, Laura Beatriz es
sedici.creator.person García, Adriana L. es
sedici.creator.person Furnus, Cecilia Cristina es
sedici.subject.materias Ciencias Médicas es
sedici.description.fulltext true es
mods.originInfo.place Facultad de Ciencias Médicas es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
sedici2003.identifier ARG-UNLP-ART-0000001056 es
sedici.relation.journalTitle Revista de la Facultad de Ciencias Médicas de la Universidad Nacional de La Plata es
sedici.relation.journalVolumeAndIssue vol. 1, no. 2 es

Download Files

This item appears in the following Collection(s)

Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) Except where otherwise noted, this item's license is described as Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)