Evaluation of increase at the production of keratinolytics enzymes

Abstract

The beamhouse area in leather technology has a notable influence on the gaseous and liquid effluent because they have to satisfies discharge standards. The organic matter content and the presence of suspended solids in the liquid effluent greatly depend on the unhairing process used: the conventional hairburning unhairing, lime and sodium sulphidebased, give the maximum values for beamhouse pollution. Nowadays, conventional unhairing process employing lime and sulphide contribute 50-60% of total pollution load in terms of biochemical oxygen demand (BOD), chemical oxygen demand (COD), total dissolved solids (TDS) and total suspended solids (TSS) besides a high alkaline effluent of 100% toxicity. Hydrogen sulphide emanating from the unhairing process is proven to be fatal evenin concentrations as low as 200 ppm. The extensive use of hazardous sulphide not onlyleads to unfavorable consequences for the environment but also undermines the efficacy of the effluent treatment plants.In the present work the enzimatic yields and proteic concentrations from crude extract ofTrichophyton ajelloi growth in solid state cultures and submerged cultivation in the presence of reducing agents were determined.