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dc.date.accessioned 2020-09-17T15:16:45Z
dc.date.available 2020-09-17T15:16:45Z
dc.date.issued 2017-10
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/104868
dc.description.abstract The aim of this study was to investigate the effects of acylated ghrelin supplementation during in vitro maturation (IVM) of bovine oocytes. IVM medium was supplemented with 20, 40 or 60 pM acylated ghrelin concentrations. Cumulus expansion area and oocyte nuclear maturation were studied as maturation parameters. Cumulus–oocyte complexes (COC) were assessed with the comet, apoptosis and viability assays. The in vitro effects of acylated ghrelin on embryo developmental capacity and embryo quality were also evaluated. Results demonstrated that acylated ghrelin did not affect oocyte nuclear maturation and cumulus expansion area. However, it induced cumulus cell (CC) death, apoptosis and DNA damage. The damage increased as a function of the concentration employed. Additionally, the percentages of blastocyst yield, hatching and embryo quality decreased with all acylated ghrelin concentrations tested. Our study highlights the importance of acylated ghrelin in bovine reproduction, suggesting that this metabolic hormone could function as a signal that prevents the progress to reproductive processes. en
dc.format.extent 601-611 es
dc.language en es
dc.subject Active ghrelin es
dc.subject Apoptosis es
dc.subject Cumulus cells es
dc.subject DNA damage es
dc.subject In vitro embryo development es
dc.title The presence of acylated ghrelin during in vitro maturation of bovine oocytes induces cumulus cell DNA damage and apoptosis, and impairs early embryo development en
dc.type Articulo es
sedici.identifier.other https://doi.org/10.1017/S0967199417000478 es
sedici.identifier.issn 1469-8730 es
sedici.creator.person Sirini, Matías Ángel es
sedici.creator.person Anchordoquy, Juan Mateo es
sedici.creator.person Anchordoquy, Juan Patricio es
sedici.creator.person Pascua, Ana Malen es
sedici.creator.person Nikoloff, Noelia es
sedici.creator.person Carranza Martín, Ana Cristina es
sedici.creator.person Relling, Alejandro Enrique es
sedici.creator.person Furnus, Cecilia Cristina es
sedici.subject.materias Ciencias Veterinarias es
sedici.subject.materias Zoología es
sedici.description.fulltext true es
mods.originInfo.place Instituto de Genética Veterinaria es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Zygote es
sedici.relation.journalVolumeAndIssue vol. 25, no. 5 es

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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Except where otherwise noted, this item's license is described as Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)