Upload resources

Upload your works to SEDICI to increase its visibility and improve its impact

 

Show simple item record

dc.date.accessioned 2020-10-07T13:48:33Z
dc.date.available 2020-10-07T13:48:33Z
dc.date.issued 2016
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/106345
dc.description.abstract The molecular basis of fluconazole resistance in Cryptococcus neoformans has been poorly studied. A common azole resistance mechanism in Candida species is the acquisition of point mutations in the ERG11 gene encoding the enzyme lanosterol 14-α-demethylase, target of the azole class of drugs. In C. neoformans only two mutations were described in this gene. In order to evaluate other mutations that could be implicated in fluconazole resistance in C. neoformans we studied the genomic sequence of the ERG11 gene in 11 clinical isolates with minimal inhibitory concentration (MIC) values to fluconazole of ≥16 μg/ml. The sequencing revealed the G1855A mutation in 3 isolates, resulting in the enzyme amino acid substitution G484S. These strains were isolated from two fluconazole-treated patients. This mutation would not intervene in the susceptibility to itraconazole and voriconazole. en
dc.description.abstract Las bases moleculares de la resistencia al fluconazol en Cryptococcus neoformans han sido poco estudiadas. Un mecanismo de resistencia a los azoles en Candida albicans es la adquisición de mutaciones puntuales en el gen ERG11, que codifica la enzima lanosterol 14 α-demetilasa, blanco de las drogas azólicas. En C. neoformans solo 2 mutaciones en este gen han sido descriptas. Con el objetivo de estudiar otras mutaciones que podrían estar implicadas en la resistencia al fluconazol en C. neoformans, realizamos la secuenciación del gen ERG11 de 11 aislamientos clínicos con valores de concentración inhibitoria mínima (CIM) de fluconazol ≥16 μg/ml. En 3 aislamientos, la secuenciación reveló la mutación G1855A, que da como resultado la sustitución aminoacídica G484S. Estos aislamientos fueron recuperados de 2 pacientes tratados con fluconazol. Esta mutación no intervendría en la sensibilidad al itraconazol y al voriconazol. es
dc.format.extent 137-142 es
dc.language en es
dc.subject Cryptococcus neoformans es
dc.subject Fluconazole resistance es
dc.subject ERG11 gene es
dc.subject amino acid substitution es
dc.subject mutation es
dc.title Amino acid substitution in Cryptococcus neoformans lanosterol 14-α-demethylase involved in fluconazole resistance in clinical isolates en
dc.title.alternative Sustitución aminoacídica en la enzima lanosterol 14 α-demetilasa de Cryptococcus neoformans involucrada en la resistencia al fluconazol de aislamientos clínicos es
dc.type Articulo es
sedici.identifier.uri https://www.sciencedirect.com/science/article/pii/S0325754116300013 es
sedici.identifier.other https://doi.org/10.1016/j.ram.2016.03.003 es
sedici.identifier.issn 0325-7541 es
sedici.creator.person Bosco Borgeat, María E. es
sedici.creator.person Mazza, Mariana es
sedici.creator.person Taverna, Constanza G. es
sedici.creator.person Córdoba, Susana Beatriz es
sedici.creator.person Murisengo, Omar A. es
sedici.creator.person Vivot, Walter es
sedici.creator.person Davel, Graciela es
sedici.subject.materias Ciencias Veterinarias es
sedici.subject.materias Ciencias Médicas es
sedici.description.fulltext true es
mods.originInfo.place Facultad de Ciencias Veterinarias es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Revista Argentina de Microbiología es
sedici.relation.journalVolumeAndIssue vol. 48, no. 2 es


Download Files

This item appears in the following Collection(s)

Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Except where otherwise noted, this item's license is described as Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)