Upload resources

Upload your works to SEDICI to increase its visibility and improve its impact

 

Show simple item record

dc.date.accessioned 2020-10-21T15:02:01Z
dc.date.available 2020-10-21T15:02:01Z
dc.date.issued 2019
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/107431
dc.description.abstract The genus Pseudomonas comprises many known plant-associated microbes with plant growth promotion and disease suppression properties. Genome-based studies allow the prediction of the underlying mechanisms using genome mining tools and the analysis of the genes unique for a strain by implementing comparative genomics. Here, we provide the genome sequence of the strain Pseudomonas brassicacearum 3Re2-7, formerly known as P. trivialis and P. reactans, elucidate its revised taxonomic classification, experimentally verify the gene predictions by transcriptome sequencing, describe its genetic biocontrol potential and contextualize it to other known Pseudomonas biocontrol agents. The P. brassicacearum 3Re2-7 genome comprises a circular chromosome with a size of 6,738,544 bp and a GC-content of 60.83%. 6267 genes were annotated, of which 6113 were shown to be transcribed in rich medium and/or in the presence of Rhizoctonia solani. Genome mining identified genes related to biocontrol traits such as secondary metabolite and siderophore biosynthesis, plant growth promotion, inorganic phosphate solubilization, biosynthesis of lipo- and exopolysaccharides, exoproteases, volatiles and detoxification. Core genome analysis revealed, that the 3Re2-7 genome exhibits a high collinearity with the representative genome for the species, P. brassicacearum subsp. brassicacearum NFM421. Comparative genomics allowed the identification of 105 specific genes and revealed gene clusters that might encode specialized biocontrol mechanisms of strain 3Re2-7. Moreover, we captured the transcriptome of P. brassicacearum 3Re2-7, confirming the transcription of the predicted biocontrol-related genes. The gene clusters coding for 2,4-diacetylphloroglucinol (phlABCDEFGH) and hydrogen cyanide (hcnABC) were shown to be highly transcribed. Further genes predicted to encode putative alginate production enzymes, a pyrroloquinoline quinone precursor peptide PqqA and a matrixin family metalloprotease were also found to be highly transcribed. With this study, we provide a basis to further characterize the mechanisms for biocontrol in Pseudomonas species, towards a sustainable and safe application of P. brassicacearum biocontrol agents. en
dc.language en es
dc.subject Pseudomonas brassicacearum es
dc.subject Genome mining es
dc.subject RNA sequencing es
dc.subject Comparative genomics es
dc.subject Biocontrol es
dc.subject Plant-growth promotion es
dc.subject Transcriptomics es
dc.title Genetic Potential of the Biocontrol Agent Pseudomonas brassicacearum (Formerly P. trivialis) 3Re2-7 Unraveled by Genome Sequencing and Mining, Comparative Genomics and Transcriptomics en
dc.type Articulo es
sedici.identifier.uri http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6722718&blobtype=pdf es
sedici.identifier.other pmid:31405015 es
sedici.identifier.other pmcid:PMC6722718 es
sedici.identifier.other https://doi.org/10.3390/genes10080601 es
sedici.identifier.issn 2073-4425 es
sedici.creator.person Nelkner, Johanna es
sedici.creator.person Torres Tejerizo, Gonzalo Arturo es
sedici.creator.person Hassa, Julia es
sedici.creator.person Lin, Timo Wentong es
sedici.creator.person Witte, Julian es
sedici.creator.person Verwaaijen, Bart es
sedici.creator.person Winkler, Anika es
sedici.creator.person Bunk, Boyke es
sedici.creator.person Spröer, Cathrin es
sedici.creator.person Overmann, Jörg es
sedici.creator.person Grosch, Rita es
sedici.creator.person Pühler, Alfred es
sedici.creator.person Schlüter, Andreas es
sedici.subject.materias Biología es
sedici.description.fulltext true es
mods.originInfo.place Instituto de Biotecnologia y Biologia Molecular es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution 4.0 International (CC BY 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Genes es
sedici.relation.journalVolumeAndIssue vol. 10, no. 8 es


Download Files

This item appears in the following Collection(s)

Creative Commons Attribution 4.0 International (CC BY 4.0) Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International (CC BY 4.0)