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dc.date.accessioned 2020-10-30T14:46:27Z
dc.date.available 2020-10-30T14:46:27Z
dc.date.issued 2018
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/108010
dc.description.abstract Background: Chagas disease is a parasitic infection caused by Trypanosoma cruzi. It is an important public health problem affecting around seven to eight million people in the Americas. A large number of hematophagous triatomine insect species, occupying diverse natural and human-modified ecological niches transmit this disease. Triatomines are long-living hemipterans that have evolved to explode different habitats to associate with their vertebrate hosts. Understanding the molecular basis of the extreme physiological conditions including starvation tolerance and longevity could provide insights for developing novel control strategies. We describe the normalized cDNA, full body transcriptome analysis of three main vectors in North, Central and South America, Triatoma pallidipennis, T. dimidiata and T. infestans. Results: Two-thirds of the de novo assembled transcriptomes map to the Rhodnius prolixus genome and proteome. A Triatoma expansion of the calycin family and two types of protease inhibitors, pacifastins and cystatins were identified. A high number of transcriptionally active class I transposable elements was documented in T. infestans, compared with T. dimidiata and T. pallidipennis. Sequence identity in Triatoma-R. prolixus 1:1 orthologs revealed high sequence divergence in four enzymes participating in gluconeogenesis, glycogen synthesis and the pentose phosphate pathway, indicating high evolutionary rates of these genes. Also, molecular evidence suggesting positive selection was found for several genes of the oxidative phosphorylation I, III and V complexes. Conclusions: Protease inhibitors and calycin-coding gene expansions provide insights into rapidly evolving processes of protease regulation and haematophagy. Higher evolutionary rates in enzymes that exert metabolic flux control towards anabolism and evidence for positive selection in oxidative phosphorylation complexes might represent genetic adaptations, possibly related to prolonged starvation, oxidative stress tolerance, longevity, and hematophagy and flight reduction. Overall, this work generated novel hypothesis related to biological adaptations to extreme physiological conditions and diverse ecological niches that sustain Chagas disease transmission. en
dc.language en es
dc.subject Chagas disease es
dc.subject Reduviid bugs es
dc.subject Transcriptome es
dc.subject metabolism es
dc.subject oxidative phosphorylation es
dc.title Adaptations in energy metabolism and gene family expansions revealed by comparative transcriptomics of three Chagas disease triatomine vectors en
dc.type Articulo es
sedici.identifier.uri http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5921304&blobtype=pdf es
sedici.identifier.other pmid:29699489 es
sedici.identifier.other pmcid:PMC5921304 es
sedici.identifier.other doi:10.1186/s12864-018-4696-8 es
sedici.identifier.issn 1471-2164 es
sedici.creator.person Martínez Barnetche, Jesús es
sedici.creator.person Lavore, Andrés E. es
sedici.creator.person Beliera, Melina es
sedici.creator.person Téllez Sosa, Juan es
sedici.creator.person Zumaya Estrada, Federico A. es
sedici.creator.person Palacio, Victorio es
sedici.creator.person Godoy Lozano, Ernestina es
sedici.creator.person Rivera Pomar, Rolando Víctor es
sedici.creator.person Rodríguez, Mario Henry es
sedici.subject.materias Ciencias Exactas es
sedici.description.fulltext true es
mods.originInfo.place Centro Regional de Estudios Genómicos es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution 4.0 International (CC BY 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle BMC Genomics es
sedici.relation.journalVolumeAndIssue vol. 19, no. 1 es


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