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dc.date.accessioned 2020-11-04T21:17:00Z
dc.date.available 2020-11-04T21:17:00Z
dc.date.issued 2016
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/108334
dc.description.abstract The aim of this study was to investigate the influence of copper (Cu) during in vitro maturation (IVM) on apoptosis and DNA integrity of cumulus cells (CC); and oocyte viability. Also, the role of CC in the transport of Cu during IVM was evaluated on oocyte developmental capacity. Damage of DNA was higher in CC matured without Cu (0 µg/dl Cu, P < 0.01) with respect to cells treated with Cu for cumulus–oocyte complexes (COCs) exposed to 0, 20, 40, or 60 µg/dl Cu). The percentage of apoptotic cells was higher in CC matured without Cu than in CC matured with Cu. Cumulus expansion and viability of CC did not show differences in COC treated with 0, 20, 40, or 60 µg/dl Cu during IVM. After in vitro fertilization (IVF), cleavage rates were higher in COC and DO + CC (denuded oocytes + CC) with or without Cu than in DO. Independently of CC presence (COC, DO + CC or DO) the blastocyst rates were higher when 60 µg/dl Cu was added to IVM medium compared to medium alone. These results indicate that Cu supplementation to IVM medium: (i) decreased DNA damage and apoptosis in CC; (ii) did not modify oocyte viability and cumulus expansion; and (iii) improved subsequent embryo development up to blastocyst stage regardless of CC presence during IVM. en
dc.format.extent 869-879 es
dc.language en es
dc.subject Apoptosis es
dc.subject DNA damage es
dc.subject Early development es
dc.subject Minerals es
dc.title Analyses of apoptosis and DNA damage in bovine cumulus cells after in vitro maturation with different copper concentration en
dc.type Articulo es
sedici.identifier.other http://dx.doi.org/10.1017/s0967199416000204 es
sedici.identifier.issn 1469-8730 es
sedici.title.subtitle Consequences on early embryo development en
sedici.creator.person Rosa, Diana Esther es
sedici.creator.person Anchordoquy, Juan Mateo es
sedici.creator.person Anchordoquy, Juan Patricio es
sedici.creator.person Sirini, Matías Ángel es
sedici.creator.person Testa, Juan Alberto es
sedici.creator.person Mattioli, Guillermo Alberto es
sedici.creator.person Furnus, Cecilia Cristina es
sedici.subject.materias Ciencias Veterinarias es
sedici.description.fulltext true es
mods.originInfo.place Instituto de Genética Veterinaria es
mods.originInfo.place Facultad de Ciencias Veterinarias es
mods.originInfo.place Facultad de Ciencias Médicas es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Zygote es
sedici.relation.journalVolumeAndIssue vol. 24, no. 6 es

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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Except where otherwise noted, this item's license is described as Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)