Blood and urine physiological values in captive bullfrog, <i>Rana catesbeiana</i> (<i>Anura: ranidae</i>)

Abstract

Con el propósito de obtener valores sanguíneos y urinarios de referencia, 302 muestras de ejemplares sanos de Rana catesbeiana del nordeste argentino (9-21 meses de edad, 50% de cada sexo), fueron analizadas por espectrofotometría, electroforesis, densitometría, refractometría y microscopía. Fueron obtenidos intervalos de confianza (p<0,05) para hematocrito (28,6-31,6%), eritrocitos (0,40-0,44 T/L), VCM (686-732 fL), hemoglobina (6,41-7,20 g/dL), HCM (151-164 pg), CHCM (22,6-24,0%), leucocitos (18,7-22,3 G/L), neutrófilos (58,4-63,4%), linfocitos (23,9-29,8%), monocitos (2,1-3,8%), eosinófilos (4,6-7,0%), basófilos (2,9-4,1%), fibrinógeno (0,59-0,99 g/dL), proteínas totales (4,19-4,49 g/dL), albúmina (1,49-1,67 g/dL), globulinas (2,64-2,97 g/dL), creatinina (4,09-5,56 mg/L), urea (76,1-92,4 mg/L), ácido úrico (11,5-15,4 mg/L), triglicéridos (0,34-0,52 g/L), colesterol total (0,56-0,67 g/L), C-HDL (0,03-0,05 g/L), C-LDL (0,34-0,44 g/L), alfa lipoproteína (6,01-8,67%), beta lipoproteína (91,3-93,9%), glucosa (0,45-0,54 g/L), Na (116-121 meq/L), K (3,42-3,81 meq/L), Cl (100-116 meq/L), Ca (7,98-8,61 mg/dL), P (8,31-9,36 mg/dL), Mg (2,26-2,55 mg/dL), Fe (105- 178 ug/dL), ALP (144-170 UI/L), ALT (10,0-14,8 UI/L), AST (42,8-53,4 UI/L), GGT (7,8-10,6 UI/L), LDH (99-135 UI/L), CHE (151-185 UI/L), CPK (365-500 UI/L), tiempo de sangría (289- 393s), tiempo de coagulación (452-696s), tiempo de protrombina (76-128s), densidad urinaria (1,0061-1,0089) y pH urinario (6,38-6,96). Algunos intervalos fueron semejantes a los obtenidos en anfibios, aves o mamíferos, pero otros resultaron muy diferentes. Ciertos parámetros registraron variaciones fisiológicas atribuibles a edad, sexo, estación del año y sistema de manejo y alimentación. Se resalta la utilidad de los parámetros estudiados para evaluar estados sanitario, metabólico y nutricional de la rana toro en cautiverio.

Samples of healthy Rana catesbeiana (302 specimens, 9-21 months-old, 50% each sex) from the north-east of Argentina, were analyzed by spectrophotometry, electrophoresis, densitometry, refractometry and microscopy in order to obtain blood and urine reference values. Confidence intervals (p<0.05) for PCV (28.6-31.6%), RBC (0.40-0.44 T/L), MCV (686- 732 fL), hemoglobin (6.41-7.20 g/dL), MCH (151-164 pg), MCHC (22.6-24.0%), WBC (18.7- 22.3 G/L), neutrophils (58.4-63.4%), lymphocytes (23.9-29.8%), monocytes (2.1-3.8%), eosinophils (4.6-7.0%), basophils (2.9-4.1%), fibrinogen (0.59-0.99 g/dL), total protein (4.19-4.49 g/dL), albumin (1.49-1.67 g/dL), globulins (2.64-2.97 g/dL), creatinine (4.09-5.56 mg/L), urea (76.1-92.4 mg/L), uric acid (11.5-15.4 mg/L), triglycerides (0.34-0.52 g/L), total cholesterol (0.56-0.67 g/L), HDL-C (0.03-0.05 g/L), LDL-C (0.34-0.44 g/L), alpha lipoprotein (6.01-8.67%), beta lipoprotein (91.3-93.9%), glucose (0.45-0.54 g/L), Na (116-121 meq/L), K (3.42-3.81 meq/ L), Cl (100-116 meq/L), Ca (7.98-8.61 mg/dL), P (8.31-9.36 mg/dL), Mg (2.26-2.55 mg/dL), Fe (105-178 ug/dL), ALP (144-170 IU/L), ALT (10.0-14.8 IU/L), AST (42.8-53.4 IU/L), GGT (7.8- 10.6 IU/L), LDH (99-135 IU/L), CHE (151-185 IU/L), CPK (365-500 IU/L), bleeding time (289- 393s), coagulation time (452-696s), prothrombin time (76-128s), urinary density (1.0061- 1.0089), and urinary pH (6,38-6.96), were obtained. Some intervals were similar to those obtained in amphibians, birds or mammals, but others were very different. Physiological variations attributable to age, sex, season, and handling and feeding system, were registered on certain parameters. The usefulness of the parameters studied to evaluate sanitary, metabolic and nutritional state on captive bullfrog should be emphasized.