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dc.date.accessioned | 2021-09-22T18:43:52Z | |
dc.date.available | 2021-09-22T18:43:52Z | |
dc.date.issued | 2021-07-17 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/125422 | |
dc.description.abstract | Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in bacterial inactivation. This study aimed to analyze, in vitro, the action of aPDT with Photodithazine® (PDZ) in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains. The strains were incubated with PDZ at 25, 50, 75, and 100 mg/L for 15 min and irradiated with fluences of 25, 50, and 100 J/cm2. The internalization of PDZ was evaluated by confocal microscopy, the bacterial growth by counting the number of colony-forming units, as well as the bacterial metabolic activity post-aPDT and the production of ROS. In both strains, the photosensitizer was internalized; the production of ROS increased when the aPDT was applied; there was a bacterial reduction compared to the control at all the evaluated fluences and concentrations; and, in most parameters, it was obtained complete inactivation with significant difference (p < 0.05). The implementation of aPDT with PDZ in clinical strains of S. aureus has resulted in its complete inactivation, including the MRSA strains. | en |
dc.language | en | es |
dc.subject | antimicrobial photodynamic therapy | es |
dc.subject | MRSA | es |
dc.subject | MSSA | es |
dc.subject | photodithazine® | es |
dc.title | Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains | en |
dc.type | Articulo | es |
sedici.identifier.uri | https://www.mdpi.com/2079-6382/10/7/869 | es |
sedici.identifier.other | doi:10.3390/antibiotics10070869 | es |
sedici.identifier.issn | 2079-6382 | es |
sedici.creator.person | Müller Nunes Souza, Beatriz | es |
sedici.creator.person | Guerra Pinto, Juliana | es |
sedici.creator.person | Correia Pereira, André Henrique | es |
sedici.creator.person | Miñán, Alejandro | es |
sedici.creator.person | Ferreira Strixino, Juliana | es |
sedici.subject.materias | Física | es |
sedici.subject.materias | Química | es |
sedici.subject.materias | Ciencias Exactas | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution 4.0 International (CC BY 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Antibiotics | es |
sedici.relation.journalVolumeAndIssue | vol. 10, no. 7 | es |