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dc.date.accessioned | 2021-11-16T13:35:17Z | |
dc.date.available | 2021-11-16T13:35:17Z | |
dc.date.issued | 2017 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/128209 | |
dc.description.abstract | Oxidative damage to proteins leads to a variety of modifications that are markers of pathogenesis. One of the most important modifications is the dityrosine (Tyr₂ ) cross-link, resulting from an oxidative covalent bond between two tyrosines (Tyr). An optimized methodology for preparation of pure Tyr₂ is important to investigate in detail its physicochemical properties and reactivity. Pterin (Ptr), the parent and unsubstituted compound of oxidized pterins, is able to photosensitize the cross-linking of free tyrosine (Tyr) and tyrosine residues of peptides and proteins through a photoinduced electron transfer mechanism. We have optimized a simple, one-step photocatalyzed formation of Tyr₂ , using Ptr as photocatalyst. Our procedure is carried out in aqueous solutions under UV-A radiation for few minutes. The purification of Tyr₂ is performed by reverse-phase chromatography. The obtained highly pure solution is used to fully characterize the Tyr₂ (exact mass and ¹H, ¹H- ¹H COSY; DEPT; HSQC and HMBC NMR experiments) and to deeper study its fluorescence properties. | en |
dc.format.extent | 67-74 | es |
dc.language | en | es |
dc.subject | Dityrosine | es |
dc.subject | Oxidation | es |
dc.subject | Cross-linking | es |
dc.subject | Pterin | es |
dc.subject | Photocatalyzed synthesis | es |
dc.subject | Photoinduced electron transfer | es |
dc.title | A novel synthetic approach to tyrosine dimers based on pterin photosensitization | en |
dc.type | Articulo | es |
sedici.identifier.other | doi:10.1016/j.dyepig.2017.07.058 | es |
sedici.identifier.issn | 0143-7208 | es |
sedici.creator.person | Reid, Lara Olivia | es |
sedici.creator.person | Castaño, Carolina | es |
sedici.creator.person | Dántola, María Laura | es |
sedici.creator.person | Lhiaubet-Vallet, Virginie | es |
sedici.creator.person | Miranda, Miguel A. | es |
sedici.creator.person | Marin, M. Luisa | es |
sedici.creator.person | Thomas, Andrés Héctor | es |
sedici.subject.materias | Química | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas | es |
sedici.subtype | Preprint | es |
sedici.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Dyes and Pigments | es |
sedici.relation.journalVolumeAndIssue | vol. 147 | es |