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dc.date.accessioned 2022-03-02T18:59:00Z
dc.date.available 2022-03-02T18:59:00Z
dc.date.issued 2020-10-20
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/131844
dc.description.abstract Ascorbic acid (AsA) and gluthathione (GSH) are two key components of the antioxidant machinery of eukaryotic and prokaryotic cells. The cyanobacterium Synechocystis sp. PCC 6803 presents both compounds in different concentrations (AsA, 20-100 μM and GSH, 2-5 mM). Therefore, it is important to have precise and sensitive methods to determine the redox status in the cell and to detect variations in this antioxidants. In this protocol, we describe an improved method to estimate the content of both antioxidants (in their reduced and oxidized forms) from the same sample obtained from liquid cultures of Synechocystis sp. PCC 6803. en
dc.language en es
dc.subject Cyanobacteria es
dc.subject Antioxidants es
dc.subject Glutathione es
dc.subject Ascorbic acid es
dc.subject Oxidative stress es
dc.title Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803 en
dc.type Articulo es
sedici.identifier.other doi:10.21769/bioprotoc.3800 es
sedici.identifier.other pmcid:PMC7842791 es
sedici.identifier.other pmid:33659454 es
sedici.identifier.issn 2331-8325 es
sedici.creator.person Aguillera, Anabella es
sedici.creator.person Steelheart Molina, María Charlotte es
sedici.creator.person Alegre, Matías Leonel es
sedici.creator.person Berdun, Federico es
sedici.creator.person Salerno, Graciela Lidia es
sedici.creator.person Bartoli, Carlos Guillermo es
sedici.creator.person Pagnussat, Gabriela Carolina es
sedici.creator.person Martin, María Victoria es
sedici.subject.materias Ciencias Naturales es
sedici.subject.materias Biología es
sedici.description.fulltext true es
mods.originInfo.place Instituto de Fisiología Vegetal es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Bio-protocol es
sedici.relation.journalVolumeAndIssue vol. 10, no. 20 es


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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Excepto donde se diga explícitamente, este item se publica bajo la siguiente licencia Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)