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dc.date.accessioned | 2022-05-20T13:49:14Z | |
dc.date.available | 2022-05-20T13:49:14Z | |
dc.date.issued | 2000 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/136614 | |
dc.description.abstract | Acetobacter diazotrophicus is a diazotrophic bacterium that colonizes sugarcane tissues. Glucose is oxidized to gluconate in the periplasm prior to uptake and metabolism. A membrane-bound glucose dehydrogenase quinoenzyme [which contains pyrroloquinoline quinone (PQQ) as the prosthetic group] is involved in that oxidation. Gluconate is oxidized further via the hexose monophosphate pathway and tricarboxylic acid cycle. A. diazotrophicus PAL3 was grown in a chemostat with atmospheric nitrogen as the sole N source provided that the dissolved oxygen was maintained at 1.0–2.0% air saturation. The biomass yields of A. diazotrophicus growing with glucose or gluconate with fixed N were very low compared with other heterotrophic bacteria. The biomass yields under N-fixing conditions were more than 30% less than with ammonium as the N source using gluconate as the carbon source but, surprisingly, were only about 14% less with glucose. The following scheme for the metabolism of A. diazotrophicus through the different pathways emerged: (1) the respiratory chain of this organism had a different efficiency of ATP production in the respiratory chain (P:O ratio) under different culture conditions; and (2) N fixation was one (but not the sole) condition under which a higher P:O ratio was observed. The other condition appears to be the expression of an active PQQ-linked glucose dehydrogenase. | en |
dc.format.extent | 564-569 | es |
dc.language | en | es |
dc.subject | Sugarcane | es |
dc.subject | Respiratory Chain | es |
dc.subject | Hexose | es |
dc.subject | Biomass Yield | es |
dc.subject | Gluconate | es |
dc.title | The carbon source influences the energetic efficiency of the respiratory chain of N2-fixing Acetobacter diazotrophicus | en |
dc.type | Articulo | es |
sedici.identifier.other | doi:10.1007/s002530000425 | es |
sedici.identifier.other | pmid:11092633 | es |
sedici.identifier.issn | 0175-7598 | es |
sedici.identifier.issn | 1432-0614 | es |
sedici.creator.person | Luna, María Flavia | es |
sedici.creator.person | Mignone, Carlos Fernando | es |
sedici.creator.person | Boiardi, José Luis | es |
sedici.subject.materias | Biología | es |
sedici.subject.materias | Química | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Centro de Investigación y Desarrollo en Fermentaciones Industriales | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Applied Microbiology and Biotechnology | es |
sedici.relation.journalVolumeAndIssue | vol. 54, no. 4 | es |