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dc.date.accessioned | 2022-07-01T17:27:25Z | |
dc.date.available | 2022-07-01T17:27:25Z | |
dc.date.issued | 2000-11-01 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/138739 | |
dc.description.abstract | ATCC 42397 produces a protopectinase (PPase-SE) with polygalacturonase (PGase) activity. The microorganism was aerobically cultivated in synthetic media. Glucose, fructose and xylose yielded the highest enzyme levels (10–11 PGase units ml−1). Galacturonic acid repressed enzyme production and no growth was obtained with disaccharides and pectin. Specific enzyme activity obtained in an O2-limited culture was similar to that found in nonlimited ones. A growth yield (Y; x/s) of 0.49 g of cell dry weight per gram of glucose consumed was obtained in a typical batch bioreactor culture. Enzyme production was growth associated, and no major products other than biomass and CO2 were detected. The volumetric enzyme activity reached a maximum around D=0.3–0.4 h−1 in glucose-limited continuous cultures. However, it varied strongly (together with microorganism morphology) even after retention times ≥8 at any D tested (0.035–0.44 h−1) though the rest of the culture variables remained fairly constant. No correlation between morphology and enzyme activity could be obtained. Enzyme production was poor in urea- and vitamin-limited continuous cultures. In all cases, biomass and CO2 accounted for ≅100% of carbon recovery though Y; x/s values were different. | en |
dc.format.extent | 260-265 | es |
dc.language | en | es |
dc.subject | Pectin | es |
dc.subject | Pectin solubilizing enzimes | es |
dc.subject | Protopectinases | es |
dc.subject | Polygalacturonases | es |
dc.subject | Geotrichum | es |
dc.title | Growth and protopectinase production of Geotrichum klebahnii in batch and continuous cultures with synthetic media | en |
dc.type | Articulo | es |
sedici.identifier.other | doi:10.1038/sj.jim.7000072 | es |
sedici.identifier.issn | 1367-5435 | es |
sedici.identifier.issn | 1476-5535 | es |
sedici.creator.person | Cavalitto, Sebastián Fernando | es |
sedici.creator.person | Hours, Roque Alberto | es |
sedici.creator.person | Mignone, Carlos Fernando | es |
sedici.subject.materias | Ciencias Exactas | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Centro de Investigación y Desarrollo en Fermentaciones Industriales | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution 4.0 International (CC BY 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Journal of Industrial Microbiology and Biotechnology | es |
sedici.relation.journalVolumeAndIssue | vol. 25, no. 5 | es |