Lipid and fatty acid composition and energy partitioning during embryo development in the shrimp Macrobrachium borellii

Abstract

Energy partitioning, composition of lipids and fatty acids, and their utilization by embryos were determined in the lecithotrophic shrimp Macrobrachium borellii during seven development stages. The biochemical composition at stage I is represented by lipids, proteins, and carbohydrates, with 29.3, 28.7, and 0.2% dry weight, respectively. The former two were identified as the major energy-providing components, contribut- ing 131 and 60 cal/100 mg egg, dry weight, respectively. The overall conversion efficiency (CE) was 45.0% (calculated as per- centage of vitelline energy transformed into embryonic tissues). Lipids were the most important energy reserve (CE 39.3%), fol- lowed by proteins (CE 57.1%), both being simultaneously uti- lized during development while carbohydrates were synthe- sized de novo (CE 587.5%). Variation in the lipid class compo- sition of embryos and vitellus showed an accumulation of triacylglycerols (TAG) and phospholipids (PL) up to stage IV, a more active accumulation and selective utilization phase (stages V and VI), and a consumption and de novo synthesis period until hatching. Structural lipids (PL and cholesterol) and pig- ment astaxanthin were selectively conserved in embryos, but TAG, hydrocarbons, and esterified sterols were preferentially depleted. Monounsaturated fatty acids (FA) were the major group in TAG, whereas polyunsaturated FA (PUFA) were the major group in PL after organogenesis. Certain PUFA such as 22:6n-3 and 20:5n-3 were selectively accumulated in PL.