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dc.date.accessioned | 2022-08-31T14:38:45Z | |
dc.date.available | 2022-08-31T14:38:45Z | |
dc.date.issued | 2003 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/141355 | |
dc.description.abstract | A new peptidase, named hieronymain I, was purified to homogeneity from unripe fruits of Bromelia hieronymi Mez (Bromeliaceae) by acetone fractionation followed by cation exchange chromatography (FPLC) on CM-Sepharose FF. Homogeneity of the enzyme was confirmed by mass spectroscopy (MALDI-TOF), isoelectric focusing, and SDS-PAGE. Hieronymain is a basic peptidase (pI > 9.3) and its molecular mass was 24,066 Da. Maximum proteolytic activity on casein (>90% of maximum activity) was achieved at pH 8.5-9.5. The enzyme was completely inhibited by E-64 and iodoacetic acid and activated by the addition of cysteine; these results strongly suggest that the isolated protease should be included within the cysteine group. The N-terminal sequence of hieronymain (ALPESIDWRAKGAVTEVKRQDG) was compared with 25 plant cysteine proteases that showed more than 50% of identity. | en |
dc.format.extent | 127-134 | es |
dc.language | en | es |
dc.subject | Bromelia hieronymi | es |
dc.subject | Bromeliaceae | es |
dc.subject | plant peptidases | es |
dc.subject | cysteine proteinase | es |
dc.title | Hieronymain I, a New Cysteine Peptidase Isolated from Unripe Fruits of Bromelia hieronymi Mez (Bromeliaceae) | en |
dc.type | Articulo | es |
sedici.identifier.other | doi:10.1023/a:1023418812832 | es |
sedici.identifier.other | pmid:12760417 | es |
sedici.identifier.issn | 0277-8033 | es |
sedici.identifier.issn | 1573-4943 | es |
sedici.creator.person | Bruno, Mariela Anahí | es |
sedici.creator.person | Pardo, Marcelo Fabián | es |
sedici.creator.person | Caffini, Néstor Oscar | es |
sedici.creator.person | López, Laura María Isabel | es |
sedici.subject.materias | Biología | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Centro de Investigación de Proteínas Vegetales | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Journal of Protein Chemistry | es |
sedici.relation.journalVolumeAndIssue | vol. 22, no. 2 | es |