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dc.date.accessioned 2022-10-03T16:34:54Z
dc.date.available 2022-10-03T16:34:54Z
dc.date.issued 2019-08-16
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/143201
dc.description.abstract This study aimed to assess the feasibility of using RFLP of PCR-amplified 16S rRNA gene (s) by using universal primers 27f/1492r and a combination of three restriction enzymes, AluI, CfoI, and TaqI, for a low-cost, rapid screen for a primarily differentiation of isolates of the complex of aerobic spore-forming bacteria commonly found in honey samples. The described method produced unique and distinguishable patterns to differentiate among 80 isolates belonging to 26 different species of Bacillus, Brevibacillus, Lysinibacillus, Rummeliibacillus, and Paenibacillus reported in honey and other apiarian sources. en
dc.language en es
dc.subject PCR-RFLP es
dc.subject Bacillus es
dc.subject Paenibacillus es
dc.subject Brevibacillus es
dc.subject Lysinibacillus es
dc.title Feasibility of using RFLP of PCR-amplified 16S rRNA gene(s) for rapid differentiation of isolates of aerobic spore-forming bacteria from honey en
dc.type Articulo es
sedici.identifier.other doi:10.1016/j.mimet.2019.105690 es
sedici.identifier.other pmid:31425714 es
sedici.identifier.issn 1872-8359 es
sedici.identifier.issn 0167-7012 es
sedici.creator.person López, Ana Claudia es
sedici.creator.person Alippi, Adriana Mónica es
sedici.subject.materias Biología es
sedici.description.fulltext true es
mods.originInfo.place Centro de Investigación y Desarrollo en Fermentaciones Industriales es
sedici.subtype Preprint es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Journal of Microbiological Methods es
sedici.relation.journalVolumeAndIssue vol. 165 es


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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Excepto donde se diga explícitamente, este item se publica bajo la siguiente licencia Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)