Protective effect of <i>Calendula Officinalis</i> L. extract against non-enzymatic peroxidation of rat kidney microsomes and mitochondria

Abstract

Cellular metabolic changes during kidney disease may induce higher production of oxygen radicals that play a significant role in the progression of renal damage and in the onset of important comorbidities. In the study reported here the effect of Calendula Officinalis L. extract (CO) on chemiluminescence of mitochondria and microsomes isolated from kidney rat was analysed. After incubation of mitochondria and microsomes in an ascorbate (0.4 mM)-Fe++ (2.15 μM) system (180 min at 37°C), non-enzymatic peroxidation, it was observed that the total cpm/mg protein originated from light emission: chemiluminescence was lower in kidney mitochondria and microsomes obtained from CO group than in the control group (without CO). Moreover, it was observed that the CO was reduced, concentration dependent (0.05, 0.25, 0.35 and 0.45 mg of extract), of chemiluminescence, measured as total cpm. The analyses of chemiluminescence indicate that CO may act as antioxidant protecting rat kidney mitochondria and microsomes from peroxidative damage.