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dc.date.accessioned 2023-05-05T17:47:08Z
dc.date.available 2023-05-05T17:47:08Z
dc.date.issued 2023
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/152539
dc.description.abstract The presence of a worn surface in the implanted material, as in the case of a replacement of a damaged osteoarticular joint, is the normal condition after implantation. This manuscript focuses precisely on the comparative study of the cellular behavior on worn CoCr surfaces, analyzing the effect of different surface modifications on macrophages’ responses. CoCr surfaces were modified by the deposition of electrochemically reduced graphene oxide (CoCrErGO), followed by additional surface functionalization with hyaluronic acid (CoCrErGOHA). After the wear corrosion processes, the macrophage response was studied. In addition, macrophage supernatants exposed to the surfaces, before and after wear, were also evaluated for osteoblast response through the analysis of the metabolic activity, plasma membrane damage, and phosphatase alkaline activity (ALP). The proteomic analysis and the quantitative TNF-α/IL-10 ratios of the J774A.1 macrophages exposed to the surfaces under study showed a polarization shift from M0 (basal state) to M1, associated with the pro-inflammatory response of all surfaces. A lower M1 polarization was observed upon exposure to the surface modification with ErGO, whereas posterior HA functionalization attenuated, even more, the M1 polarization. The wear corrosion process contributed to inflammation and exacerbated the M1 polarization response on macrophages to CoCr, which was diminished for the ErGO and attenuated the most for the ErGOHA surfaces. Comparative proteomics showed that the pathways related to M1 polarization were downregulated on the surfaces of CoCrErGOHA, which suggests mechanisms for the observed attenuation of M1 polarization. The suitable immuno-modulatory potential induced by the ErGOHA surface, with and without wear, together with the stimulation of ALP activity in osteoblasts induced by macrophage supernatants, promotes the mineralization processes necessary for bone repair. This makes it feasible to consider the adsorption of ErGOHA on CoCr as a recommended surface treatment for the use of biomaterials in osseous joint applications. en
dc.language en es
dc.subject CoCr es
dc.subject Proteomic analysis es
dc.subject Inflammatory response es
dc.subject Wear es
dc.subject Graphene es
dc.subject Hyaluronic acid es
dc.title Effect of wear-corrosion of reduced graphene oxide functionalized with hyaluronic acid on inflammatory and proteomic response of J774A.1 macrophages en
dc.type Articulo es
sedici.identifier.other https://doi.org/10.3390/met13030598 es
sedici.identifier.issn 2075-4701 es
sedici.creator.person Sánchez López, Luna es
sedici.creator.person Ropero de Torres, Noelia es
sedici.creator.person Chico, Belén es
sedici.creator.person Fagali, Natalia es
sedici.creator.person Ríos, Vivian de los es
sedici.creator.person Escudero,María Lorenza es
sedici.creator.person García Alonso, María Cristina es
sedici.creator.person Lozano, Rosa María es
sedici.subject.materias Química es
sedici.description.fulltext true es
mods.originInfo.place Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution 4.0 International (CC BY 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Metals es
sedici.relation.journalVolumeAndIssue vol. 13, no. 3 es


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Creative Commons Attribution 4.0 International (CC BY 4.0) Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International (CC BY 4.0)