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dc.date.accessioned 2023-05-12T18:30:57Z
dc.date.available 2023-05-12T18:30:57Z
dc.date.issued 2023
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/152929
dc.description.abstract We aimed to assess the potential of baculoviral vectors (BV) for brain cancer gene therapy. We compared them with adenoviral vectors (AdV), which are used in neuro-oncology, but for which there is pre-existing immunity. We constructed BVs and AdVs encoding fluorescent reporter proteins and evaluated their transduction efficiency in glioma cells and astrocytes. Naïve and glioma-bearing mice were intracranially injected with BVs to assess transduction and neuropathology. Transgene expression was also assessed in the brain of BV-preimmunized mice. While the expression of BVs was weaker than AdVs in murine and human glioma cell lines, BV-mediated transgene expression in patient-derived glioma cells was similar to AdV-mediated transduction and showed strong correlation with clathrin expression, a protein that interacts with the baculovirus glycoprotein GP64, mediating BV endocytosis. BVs efficiently transduced normal and neoplastic astrocytes in vivo, without apparent neurotoxicity. BV-mediated transgene expression was stable for at least 21 days in the brain of naïve mice, but it was significantly reduced after 7 days in mice systemically preimmunized with BVs. Our findings indicate that BVs efficiently transduce glioma cells and astrocytes without apparent neurotoxicity. Since humans do not present pre-existing immunity against BVs, these vectors may constitute a valuable tool for the delivery of therapeutic genes into the brain. en
dc.language en es
dc.subject Baculoviruses es
dc.subject Glioblastoma es
dc.subject Astrocytes es
dc.subject Gene therapy es
dc.subject Brain es
dc.title Evaluation of baculoviruses as gene therapy vectors for brain cancer en
dc.type Articulo es
sedici.identifier.other https://doi.org/10.3390/v15030608 es
sedici.identifier.issn 1999-4915 es
sedici.creator.person Garcia Fallit, Matías es
sedici.creator.person Pidre, Matías Luis es
sedici.creator.person Asad, Antonela S. es
sedici.creator.person Peña Agudelo, Jorge A. es
sedici.creator.person Vera, Mariana B. es
sedici.creator.person Nicola Candia, Alejandro J. es
sedici.creator.person Sagripanti, Sofia B. es
sedici.creator.person Pérez Kuper, Melanie es
sedici.creator.person Amorós Morales, Leslie Cinthya es
sedici.creator.person Marchesini, Abril es
sedici.creator.person Gonzalez, Nazareno es
sedici.creator.person Caruso, Carla M. es
sedici.creator.person Romanowski, Víctor es
sedici.creator.person Seilicovich, Adriana es
sedici.creator.person Videla Richardson, Guillermo A. es
sedici.creator.person Zanetti, Flavia A. es
sedici.creator.person Candolfi, Marianela es
sedici.subject.materias Biología es
sedici.subject.materias Ciencias Médicas es
sedici.description.fulltext true es
mods.originInfo.place Instituto de Biotecnología y Biología Molecular es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution 4.0 International (CC BY 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Viruses es
sedici.relation.journalVolumeAndIssue vol. 15, no. 3 es


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Creative Commons Attribution 4.0 International (CC BY 4.0) Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International (CC BY 4.0)