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dc.date.accessioned | 2023-05-12T18:30:57Z | |
dc.date.available | 2023-05-12T18:30:57Z | |
dc.date.issued | 2023 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/152929 | |
dc.description.abstract | We aimed to assess the potential of baculoviral vectors (BV) for brain cancer gene therapy. We compared them with adenoviral vectors (AdV), which are used in neuro-oncology, but for which there is pre-existing immunity. We constructed BVs and AdVs encoding fluorescent reporter proteins and evaluated their transduction efficiency in glioma cells and astrocytes. Naïve and glioma-bearing mice were intracranially injected with BVs to assess transduction and neuropathology. Transgene expression was also assessed in the brain of BV-preimmunized mice. While the expression of BVs was weaker than AdVs in murine and human glioma cell lines, BV-mediated transgene expression in patient-derived glioma cells was similar to AdV-mediated transduction and showed strong correlation with clathrin expression, a protein that interacts with the baculovirus glycoprotein GP64, mediating BV endocytosis. BVs efficiently transduced normal and neoplastic astrocytes in vivo, without apparent neurotoxicity. BV-mediated transgene expression was stable for at least 21 days in the brain of naïve mice, but it was significantly reduced after 7 days in mice systemically preimmunized with BVs. Our findings indicate that BVs efficiently transduce glioma cells and astrocytes without apparent neurotoxicity. Since humans do not present pre-existing immunity against BVs, these vectors may constitute a valuable tool for the delivery of therapeutic genes into the brain. | en |
dc.language | en | es |
dc.subject | Baculoviruses | es |
dc.subject | Glioblastoma | es |
dc.subject | Astrocytes | es |
dc.subject | Gene therapy | es |
dc.subject | Brain | es |
dc.title | Evaluation of baculoviruses as gene therapy vectors for brain cancer | en |
dc.type | Articulo | es |
sedici.identifier.other | https://doi.org/10.3390/v15030608 | es |
sedici.identifier.issn | 1999-4915 | es |
sedici.creator.person | Garcia Fallit, Matías | es |
sedici.creator.person | Pidre, Matías Luis | es |
sedici.creator.person | Asad, Antonela S. | es |
sedici.creator.person | Peña Agudelo, Jorge A. | es |
sedici.creator.person | Vera, Mariana B. | es |
sedici.creator.person | Nicola Candia, Alejandro J. | es |
sedici.creator.person | Sagripanti, Sofia B. | es |
sedici.creator.person | Pérez Kuper, Melanie | es |
sedici.creator.person | Amorós Morales, Leslie Cinthya | es |
sedici.creator.person | Marchesini, Abril | es |
sedici.creator.person | Gonzalez, Nazareno | es |
sedici.creator.person | Caruso, Carla M. | es |
sedici.creator.person | Romanowski, Víctor | es |
sedici.creator.person | Seilicovich, Adriana | es |
sedici.creator.person | Videla Richardson, Guillermo A. | es |
sedici.creator.person | Zanetti, Flavia A. | es |
sedici.creator.person | Candolfi, Marianela | es |
sedici.subject.materias | Biología | es |
sedici.subject.materias | Ciencias Médicas | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Instituto de Biotecnología y Biología Molecular | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution 4.0 International (CC BY 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Viruses | es |
sedici.relation.journalVolumeAndIssue | vol. 15, no. 3 | es |