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dc.date.accessioned 2023-05-19T16:28:41Z
dc.date.available 2023-05-19T16:28:41Z
dc.date.issued 2007
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/153291
dc.description.abstract Bombyx mori silk fibroin self-assembles on surfaces to form ultrathin nanoscale coatings based on our prior studies using layer-bylayer deposition techniques driven by hydrophobic interactions between silk fibroin protein molecules. In the present study, poly(lacticco- glycolic acid) (PLGA) and alginate microspheres were used as substrates and coated with silk fibroin. The coatings were visualized by confocal laser scanning microscopy using fluorescein-labeled silk fibroin. On PLGA microspheres, the coating was ∼1 μm and discontinuous, reflecting the porous surface of these microspheres determined by SEM. In contrast, on alginate microspheres the coating was ∼10 μm thick and continuous. The silk fibroin penetrated into the alginate gel matrix. The silk coating on the PLGA microspheres delayed PLGA degradation. The silk coating on the alginate microspheres survived ethylenediamine tetraacetic acid (EDTA) treatment used to remove the Ca2+-cross-links in the alginate gels to solubilize the alginate. This suggests that alginate microspheres can be used as templates to form silk microcapsules. Horseradish peroxidase (HRP) and tetramethylrhodamine-conjugated bovine serum albumin (Rh-BSA) as model protein drugs were encapsulated in the PLGA and alginate microspheres with and without the silk fibroin coatings. Drug release was significantly retarded by the silk coatings when compared to uncoated microsphere controls, and was retarded further by methanol-treated silk coating when compared to silk water-based coatings on alginate microspheres. Silk coatings on PLGA and alginate microspheres provide mechanically stable shells as well as a diffusion barrier to the encapsulated protein drugs. This coating technique has potential for biosensor and drug delivery applications due to the aqueous process employed, the ability to control coating thickness and crystalline content, and the biocompatibility of the silk fibroin protein used in the process. en
dc.format.extent 4161-4169 es
dc.language en es
dc.subject Silk es
dc.subject Fibroin es
dc.subject Alginate es
dc.subject Polylactic acid es
dc.subject Polyglycolic acid es
dc.subject Controlled release es
dc.title Silk coatings on PLGA and alginate microspheres for protein delivery en
dc.type Articulo es
sedici.identifier.other https://doi.org/10.1016/j.biomaterials.2007.05.036 es
sedici.identifier.issn 1878-5905 es
sedici.identifier.issn 0142-9612 es
sedici.creator.person Wang, Xiaoqin es
sedici.creator.person Wenk, Esther es
sedici.creator.person Hu, Xiao es
sedici.creator.person Castro, Guillermo Raúl es
sedici.creator.person Meinel, Lorenz es
sedici.creator.person Wang, Xianyan es
sedici.creator.person Li, Chunmei es
sedici.creator.person Merkle, Hans es
sedici.creator.person Kaplan, David L. es
sedici.subject.materias Bioquímica es
sedici.description.fulltext true es
mods.originInfo.place Centro de Investigación y Desarrollo en Fermentaciones Industriales es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Biomaterials es
sedici.relation.journalVolumeAndIssue vol. 28, no. 28 es

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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Except where otherwise noted, this item's license is described as Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)