Enteropatía proliferativa porcina

Abstract

Los objetivos de este trabajo fueron: determinar la seroprevalencia de L. intracellularis en granjas intensivas en confinamiento; analizar los factores de riesgo relacionados con la enteropatía proliferativa porcina -EPP-; realizar, en granjas seropositivas, estudios serológicos longitudinales y transversales; comparar las técnicas de histoquímica e inmunohistoquímica; determinar la prevalencia de lesiones en friogríficos; realizar la inmunomarcación de células apoptóticas en muestras de íleon en casos de campo y experimentales de EPP; describier el patrón lectinhistoquímico -LHQ- de casos experimentales de EPP a diferentes días de postinoculación.

The aims of the study were: to investigative the seroprevalence of L. intracellularis infection in intensive managemente Argentine pig farms; to assess the risk factors associated with the herdïs serological status; in positive herds, to evaluate the seroprofile by using longitudinal and cross sectional studies; to compare histochemistry -Warthin Starry stain- and inmunohistochemistry -IHC- tiiks fir tge vusyakuzatuib ir udebtufucatuib if K. intracellularis from suspected cases of porcine proliferative enteropthies -PPE-; to evaluate the prevalence of PPE lesions by histology, WS, IHC in ileum samples from seropositive hers obtained at slaughterhoses: to detect by IHC, TUNEL method, the apoptotic cells in ileum samples from naturally and experimentally acquired lesions of PPE and finally to investigate the expression of carbohydrates of ileal enterocytes, from inoculated pigs at different days postinoculation -dpi- by a panel of 10 biotinilated lectins.