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dc.date.accessioned 2023-11-10T13:08:10Z
dc.date.available 2023-11-10T13:08:10Z
dc.date.issued 2023
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/160011
dc.description.abstract Baculoviruses are insect-specific pathogens widely used in biotechnology. In particular, the Autographa californica nucleopolyhedrovirus (AcMNPV) has been exploited as a platform for bio-inputs production. This is why the improvement of the technologies used for the production of recombinant baculoviruses takes on particular relevance. To achieve this goal, we developed a highly versatile baculoviral transfer vector generation system called PluriBAC. The PluriBAC system consists of three insert entry levels using Golden Gate assembly technology. The wide availability of vectors and sticky ends allows enough versatility to combine more than four different promoters, genes of interest, and terminator sequences. Here, we report not only the rational design of the PluriBAC system but also its use for the generation of baculoviral reporter vectors applied to different fields of biotechnology. We demonstrated that recombinant AcMNPV baculoviruses generated with the PluriBAC system were capable of infecting Spodoptera frugiperda larvae. On the other hand, we found that the recombinant budded virions (BV) generated using our system were capable of transducing different types of tumor and normal cells both in vitro and in vivo. Our findings suggest that the PluriBAC system could constitute a versatile tool for the generation of insecticide and gene therapy vectors. en
dc.language es es
dc.subject baculovirus expression vectors es
dc.subject golden gate assembly es
dc.subject bio-inputs es
dc.subject gene therapy es
dc.title PluriBAC: a versatile baculovirus-based modular system to express heterologous genes in different biotechnological platforms en
dc.type Articulo es
sedici.identifier.other https://doi.org/10.3390/v15101984 es
sedici.identifier.issn 1999-4915 es
sedici.creator.person Amorós Morales, Leslie Cinthya es
sedici.creator.person Marchesini, Abril es
sedici.creator.person Gómez Bergna, Santiago Manuel es
sedici.creator.person García Fallit, Matías es
sedici.creator.person Tongiani, Silvana E. es
sedici.creator.person Vásquez, Larisa es
sedici.creator.person Ferrelli, María Leticia es
sedici.creator.person Videla-Richardson, Guillermo A. es
sedici.creator.person Candolfi, Marianela es
sedici.creator.person Romanowski, Víctor es
sedici.creator.person Pidre, Matías Luis es
sedici.subject.materias Biología es
sedici.description.fulltext true es
mods.originInfo.place Instituto de Biotecnología y Biología Molecular es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution 4.0 International (CC BY 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Viruses es
sedici.relation.journalVolumeAndIssue vol. 15, no. 10 es


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Creative Commons Attribution 4.0 International (CC BY 4.0) Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International (CC BY 4.0)