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dc.date.accessioned 2012-05-14T18:08:24Z
dc.date.available 2012-05-14T18:08:24Z
dc.date.issued 2012
dc.identifier.uri http://hdl.handle.net/10915/18097
dc.description.abstract A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of cilostazol in rat plasma using one-step protein precipitation was developed. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on an SB-C18 (2.1 × 50 mm, 5.0 μm) column with acetonitrile-0.1 % formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 370.0 for cilostazol and m/z 325.9 for the IS. Calibration plots were linear over the range of 10-2000 ng/mL for cilostazol in rat plasma. Lower limit of quantification (LLOQ) for cilostazol was 10 ng/mL. Mean recovery of cilostazol from plasma was in the range 90.14- 95.10 %. RSD of intra-day and inter-day precision were both less than 15 %. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of cilostazol in rat plasma. es
dc.format.extent p. 240-244 es
dc.language en es
dc.title Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application es
dc.type Articulo es
sedici.identifier.uri http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_1_10.pdf es
sedici.creator.person Wang, Jinjin es
sedici.creator.person Wang, Zhibin es
sedici.creator.person Wang, Zhiyi es
sedici.creator.person Chen, Chan es
sedici.creator.person Liu, Naihua es
sedici.creator.person Ma, Jianshe es
sedici.creator.person Wang, Xianqin es
sedici.creator.person Hu, Lufeng es
sedici.subject.materias Farmacia es
sedici.subject.decs cilostazo es
sedici.subject.keyword cilostazol; gradient elution; LC-ESI-MS; rat plasma es
sedici.description.fulltext true es
mods.originInfo.place Colegio de Farmacéuticos de la Provincia de Buenos Aires es
sedici.subtype Articulo es
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Latin American Journal of Pharmacy
sedici.relation.journalVolumeAndIssue vol. 31, no. 02


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