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dc.date.accessioned 2012-05-17T19:50:26Z
dc.date.available 2012-05-17T19:50:26Z
dc.date.issued 2012
dc.identifier.uri http://hdl.handle.net/10915/18144
dc.description.abstract A selective liquid chromatography-mass spectrometry (LC–MS) method for determination of febuxostat in rat plasma was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation, and chromatography involved Agilent SB-C18 column (2.1 x150 mm, 5 μm) using 0.1% formic acid in water and acetonitrile as a mobile phase with gradient elution. Detection involved positive ion mode electrospray ionization (ESI), and selective ion monitoring (SIM) mode was used for quantification of target fragment ions m/z 317 for febuxostat and m/z 326 for midazolam (internal standard, IS). The assay was linear over the range of 10-2000 ng/mL for febuxostat, with a lower limit of quantitation (LLOQ) of 10 ng/mL for febuxostat. Intra- and inter-day RSDs were less than 15% and the accuracies were in the range of 93.8-111.9% for febuxostat. This developed method was successfully applied to determinate of febuxostat in rat plasma for pharmacokinetic study. es
dc.format.extent p. 321-325 es
dc.language en es
dc.title Development and Validation of Liquid Chromatography-Mass Spectrometry Method for Determination of Febuxostat in Rat Plasma and its Application es
dc.type Articulo es
sedici.identifier.uri http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_2_4.pdf es
sedici.identifier.issn 0326-2383 es
sedici.creator.person Ding, Xitao es
sedici.creator.person Zhang, Qingwei es
sedici.creator.person Wang, Zhiyi es
sedici.creator.person Lin, Guanyang es
sedici.subject.materias Farmacia es
sedici.subject.decs Farmacología es
sedici.subject.decs Farmacocinética es
sedici.subject.keyword LC-MS; febuxostat; pharmacokinetics;rat plasma es
sedici.description.fulltext false es
mods.originInfo.place Colegio de Farmacéuticos de la Provincia de Buenos Aires es
sedici.subtype Comunicacion es
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Latin American Journal of Pharmacy es
sedici.relation.journalVolumeAndIssue vol. 31, no. 02 es


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