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dc.date.accessioned 2012-08-29T11:11:58Z
dc.date.available 2012-08-29T11:11:58Z
dc.date.issued 2012
dc.identifier.uri http://hdl.handle.net/10915/20243
dc.description.abstract Alpinetin, a type of novel plant flavonoid derived from Alpinia katsumadai Hayata, has been demonstrated to exhibit multiple biochemical and pharmacological activities. The phenyl hydroxyl group existed in alpinetin is susceptible to the glucuronidation catalyzed by UDP-glucuronosyltransferase (UGT). The aim of the present study is to develop a sensitive and specific ultra-fast liquid chromatography (UFLC)-mass spectrum (MS) method to determine the glucuronidation activity of alpinetin in the liver microsomes obtained from different species, including human liver microsomes (HLMs), rat liver microsomes (RLMs), mice liver microsomes (MLMs), and dog liver microsomes (DLMs). The alpinetin’s glucuronide was purified, and the structure was elucidated using 1H-NMR and 13C-NMR. The mass spectrometric detection was performed under selected ion monitoring (SIM) for alpinetin glucuronide at m/z 445 and 4-methylumbelliferyl-β-glucuronide (I.S.) at m/z 351. The assay exhibited linearity over the range 0.1- 60 μM for alpinetin glucuronide with the correlation coefficient of 0.9991. The intra- and inter-day precision was less than 5.3 %, with accuracy in the range 105.1-106.8 %. The developed method has been successfully applied to determine the glucuronidation of alpinetin, and the intrinsic clearance was calculated to be 2.51, 1.29, 0.23, and 1.27 mL/min/mg pro for HLM, RLM, MLM and DLM, respectively. The results obtained from this study are helpful to selection of animal models for pre-clinical pharmacokinetic study. en
dc.format.extent p. 785-789 es
dc.language en es
dc.title Determination of alpinetin glucuronidation activities in liver microsomes from different species using UFLC-ESI-MS en
dc.type Articulo es
sedici.identifier.uri http://www.latamjpharm.org/resumenes/31/5/LAJOP_31_5_2_11.pdf es
sedici.identifier.issn 0326-2383
sedici.creator.person Wang, Zheng-Hua es
sedici.creator.person Hong, Mo es
sedici.creator.person Sun, Xiao-Yu es
sedici.creator.person He, Gui-Yuan es
sedici.subject.materias Farmacia es
sedici.subject.decs Microsomas es
sedici.subject.keyword Alpinetin en
sedici.subject.keyword Glucuronidation en
sedici.subject.keyword Species difference en
sedici.subject.keyword UFLC-MS en
sedici.description.fulltext false es
mods.originInfo.place Colegio de Farmacéuticos de la Provincia de Buenos Aires es
sedici.subtype Comunicacion es
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Latin American Journal of Pharmacy es
sedici.relation.journalVolumeAndIssue vol. 31, no. 5 es


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