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dc.date.accessioned 2013-01-04T12:56:14Z
dc.date.available 2013-01-04T12:56:14Z
dc.date.issued 2012
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/25754
dc.description.abstract Bendamustine is a multifunctional alkylating agent for the treatment of multiple myeloma, with the G2/M arrest-induction ability in human multiple myeloma RPMI-8226 cells, but the mechanism remains ambiguous. In this study, we found bendamustine caused the G2 arrest in 24 h, regulated the phosphorylation status of Cdc2, and blocked the nuclear import of Cdc2-CyclinB1 complex. Pretreatment with ATM/ATR inhibitor caffeine or p38 MAPK inhibitor SB203580 suppressed the phosphorylation of Cdc2 at Thr14/Tyr15 or attenuate the blockade of nuclear import, respectively; however, neither of these two inhibitors nor the combination imposed significant effects on Bendamustine-triggered G2 arrest. Bendamustine-induced blockade of the nuclear translocation dissipated after 48 h, after which, the G2 arrest was maintained through the inhibitory phosphorylation of Cdc2. Taken together, our research suggested that two or more pathways and mechanisms which regulated the cell cycle in a time-dependent manner were involved in the G2 arrest invoked by bendamustine. es
dc.language en es
dc.subject Bendamustine en
dc.subject Cdc2-CyclinB1 complex en
dc.subject G2 arrest en
dc.subject RPMI-8226 en
dc.title Regulation of subcellular location and activity of Cdc2-cyclinb1 is involved in bendamustine-induced G2 arrest en
dc.type Articulo es
sedici.identifier.uri http://www.latamjpharm.org/resumenes/31/7/LAJOP_31_7_1_18.pdf es
sedici.identifier.issn 0326-2383
sedici.creator.person Lin, Guan-Yu es
sedici.creator.person He, Ling-Juan es
sedici.creator.person Yan, Yan es
sedici.creator.person Wang, Duo-Duo es
sedici.creator.person Zhuang, Kai-Zan es
sedici.creator.person Zhu, Hong es
sedici.creator.person Yang, Bo es
sedici.subject.materias Farmacia es
sedici.description.fulltext false es
mods.originInfo.place Colegio de Farmacéuticos de la Provincia de Buenos Aires es
sedici.subtype Articulo es
sedici.description.peerReview peer-review es
sedici.relation.journalTitle Latin American Journal of Pharmacy es
sedici.relation.journalVolumeAndIssue vol. 31, no. 7 es

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