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Mostrar registro sencillo 2018-05-16T17:56:27Z 2018-05-16T17:56:27Z 2013
dc.description.abstract To further our understanding of the virulence potential of Bacillus megaterium strains, cell association and invasion assays were conducted in vitro by infecting human enterocytes (Caco-2 cells) with 53 strains of this bacterium isolated from honey. Two series of experiments were performed: (i) necrosis and cell detachment assays with the supernatants of bacterial culture fi ltrates from 16-h cultures and (ii) adhesion/invasion assays in which cultured enterocytes incubated with bacteria from 3-h cultures were resuspended in Dulbecco’s modifi ed Eagle’s medium and chloramphenicol. The detachment of Caco-2 cells was evaluated by staining the cells with crystal violet. Necrosis was assessed by fl uorescence microscopy of cells labeled with propidium iodide. Association (adhesion plus invasion) was determined by plate counts and invasion in an aminoglycoside protection assay. The results showed that spent culture supernatants detached and necrotized Caco-2 cells in a strain-dependent manner. Seven out of 53 B. megaterium fi ltered culture supernatants caused complete cell detachment. Suspensions of these same bacterial strains adhered and invaded enterocytes in 2-h infection experiments. To our knowledge, this is the fi rst report on the interaction between B. megaterium and intestinal epithelial Caco-2 cells. en
dc.format.extent p. 27-33 es
dc.language en es
dc.title In vitro interaction between Bacillus megaterium strains and Caco-2 cells en
dc.type Articulo es
sedici.identifier.uri es
sedici.identifier.doi 10.2436/20.1501.01.177
sedici.identifier.issn 1139-6709 es
sedici.creator.person López, Ana Clara es
sedici.creator.person Minnaard, Jessica es
sedici.creator.person Pérez, Pablo Fernando es
sedici.creator.person Alippi, Adriana Mónica es
sedici.subject.materias Ciencias Agrarias es
sedici.subject.decs Bacterias es
sedici.subject.keyword Bacillus megaterium; Caco-2 cells; adhesion; cellular necrosis; spent culture supernatant; honey en
sedici.description.fulltext true es Facultad de Ciencias Agrarias y Forestales es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
sedici.description.peerReview peer-review es
sedici.relation.journalTitle International Microbiology es
sedici.relation.journalVolumeAndIssue vol. 16 es

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