Efectos de un hidrocarburo aromático policíclico (ß naftoflavona) sobre biomarcadores de efecto en Corydoras paleatus en condiciones de campo y laboratorio

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are important anthropogenic sources of aquatic contamination. In this study, hepatic biomarker responses of Corydoras paleatus injected with one sublethal dose of a PAH (ß-naftoflavona, BNF) were assessed under laboratory and field conditions. Glutathion-S-transferase (GST) and catalase (CAT) hepatic activities, liver protein content (PC), condition factor (CF) and liver somatic index (LSI) were determined. Two bioassays were performed during winter time: laboratory (la) and field (ca). Fish were injected with 50 mg BNF/kg body weight dissolved in corn oil (ß-la y ßca); control fish received corn oil (C-la y C-ca). There were no differences between controls or in BNF injected fish in the assessed parameters. BNF provoked a tendency of increase in CAT and GST activities and lower values of CP; on the other hand, no differences were observed in the FC and IHS. In BNF field exposed fish (ß-ca) CAT activity was significantly stimulated vs. both field and laboratory controls; GST differences were detected both between ß-ca and ß-la and their respective controls. BNF exposure induced adverse effects principally in the enzymatic biomarkers of C. paleatus and those effects were modulated by the environmental conditions.