Antiglycation, antiplatelets aggregation, cytotoxic and phytotoxic activities of Nepeta suavis

Abstract

Nepeta suavis Stapf. (Lamiaceae), one of the ignored species for testing biological activities, was studied. In present research, the Nepeta suavis fractions: chloroform (FC), ethyl acetate (FE) and aqueous (FW) were evaluated for platelet aggregation, antiglycation, cytoxicity, and phytotoxicity. FE showed 65.60% antiglycation activity against the protein glycation while the other fractions showed less than 50% inhibitory potential. The FW inhibited arachidonic acid (AA) and platelet activating factor (acetyl-glyceryl-ether-phosphorylcholine, PAF) induced platelet aggregation. FE showed significant cytotoxicity against brine shrimp larvae with LD50 of 41.3 μg/ml. Phytotoxic studies of FC, FE and FW against Lemna minor showed 77.5-100% inhibitory effects at 1000 μg/ml. However, at lower concentration (10 μg/ml) enhancing effects were observed in FC and FE, as compared to control. FW remained in a uniform pattern of inhibitory effects in all three concentrations (10,100 and 1000 μg/ml). FE showed highest inhibitory activities against formation of glycation, while FW showed significant inhibitory effects against platelet aggregation and Lemna minor. Both of these fractions are recommended for further study to identify and isolate active chemical compounds.