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dc.date.accessioned 2011-03-01T17:42:37Z
dc.date.available 2011-03-01T03:00:00Z
dc.date.issued 2011 es
dc.identifier.uri http://hdl.handle.net/10915/8094
dc.description.abstract A selective and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for quantitation of bupropion hydrochloride in rat plasma using triazolam as an internal standard. Chromatographic separation was achieved on a SB-C18 column at 30 °C, with 50: 50 (v/v) acetonitrile-0.1 % formic acid in water as mobile phase. The flow rate was 0.3 mL/min. The determination of bupropion was performed in MRM mode, m/z 239.9 → 183.7 for bupropion and m/z 343.0 → 308.0 for triazolam (IS) and positive ion electrospray ionization interface. Calibration curve was linear over range of 1.2 to 480 ng/mL. The intra- and inter-run relative standard deviations of the assay were less than 10 %. The mean absolute recoveries determined at the concentrations of 2.4, 48 and 360 ng/mLwere 91.00%, 92.06%, 91.71%, respectively. The validated method is successfully used to analyze the drug in samples of rat plasma for pharmacokinetic study. es
dc.format.extent p. 10-16 es
dc.language en es
dc.title Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study es
dc.type Articulo es
sedici.identifier.uri http://www.latamjpharm.org/resumenes/30/1/LAJOP_30_1_1_2.pdf es
sedici.creator.person Hu, Lufeng es
sedici.creator.person Lin, Guanyang es
sedici.creator.person Wang, Xianqin es
sedici.creator.person Xu, Ren-ai es
sedici.creator.person Zhang, Xiuhua es
sedici.creator.person Zhang, Chunhong es
sedici.subject.materias Farmacia es
sedici.subject.decs Fenetilaminas es
sedici.subject.decs Farmacocinética es
sedici.subject.decs Ratas es
sedici.subject.descriptores Cromatografía líquida es
sedici.subject.keyword bupropion hydrochloride; LC-MS/MS; rat plasma es
sedici.description.fulltext false es
mods.originInfo.place Colegio de Farmacéuticos de la Provincia de Buenos Aires es
sedici.subtype Articulo es
sedici.description.peerReview peer-review es
sedici2003.identifier ARG-FARM-ART-0000001581 es
sedici.relation.journalTitle Latin American Journal of Pharmacy es
sedici.relation.journalVolumeAndIssue vol. 30, no. 1 es


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