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dc.date.accessioned | 2019-10-09T18:46:08Z | |
dc.date.available | 2019-10-09T18:46:08Z | |
dc.date.issued | 2006 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/82988 | |
dc.description.abstract | The effects of growth irradiance and respiration on ascorbic acid (AA) synthesis and accumulation were studied in the leaves of wild-type and transformed Arabidopsis thaliana with modified amounts of the mitochondrial alternative oxidase (AOX) protein. Plants were grown under low (LL; 50 μmol photons m-2 s-1), intermediate (IL; 100 μmol photons m-2 s-1), or high (HL; 250 μmol photons m-2 s-1) light. Increasing growth irradiance progressively elevated leaf AA content and hence the values of dark-induced disappearance of leaf AA, which were 11, 55, and 89 nmol AA lost g-1 fresh weight h-1, from LL-, IL-, and HL-grown leaves, respectively. When HL leaves were supplied with L-galactone-1,4-lactone (L-GalL; the precursor of AA), they accumulated twice as much AA and had double the maximal L-galactone-1,4-lactone dehydrogenase (L-GalLDH) activities of LL leaves. Growth under HL enhanced dehydroascorbate reductase and monodehydroascorbate reductase activities. Leaf respiration rates were highest in the HL leaves, which also had higher amounts of cytochrome c and cytochrome c oxidase (CCO) activities, as well as enhanced capacity of the AOX and CCO electron transport pathways. Leaves of the AOX-overexpressing lines accumulated more AA than wild-type or antisense leaves, particularly at HL. Intact mitochondria from AOX-overexpressing lines had higher AA synthesis capacities than those from the wild-type or antisense lines even though they had similar L-GalLDH activities. AOX antisense lines had more cytochrome c protein than wild-type or AOX-overexpressing lines. It is concluded that regardless of limitations on L-GalL synthesis by regulation of early steps in the AA synthesis pathway, the regulation of L-GalLDH activity via the interaction of light and respiratory controls is a crucial determinant of the overall ability of leaves to produce and accumulate AA. | en |
dc.format.extent | 1621-1631 | es |
dc.language | en | es |
dc.subject | Alternative oxidase | es |
dc.subject | Ascorbic acid | es |
dc.subject | Cytochrome c | es |
dc.subject | Light acclimation | es |
dc.subject | Mitochondria | es |
dc.subject | Respiration | es |
dc.title | Inter-relationships between light and respiration in the control of ascorbic acid synthesis and accumulation in Arabidopsis thaliana leaves | en |
dc.type | Articulo | es |
sedici.identifier.other | doi:10.1093/jxb/erl005 | es |
sedici.identifier.other | eid:2-s2.0-33745630643 | es |
sedici.identifier.issn | 0022-0957 | es |
sedici.creator.person | Bartoli, Carlos Guillermo | es |
sedici.creator.person | Yu, Jianping | es |
sedici.creator.person | Gomez, Facundo Martin | es |
sedici.creator.person | Fernández, Laura Virginia | es |
sedici.creator.person | McIntosh, Lee | es |
sedici.creator.person | Foyer, Christine H. | es |
sedici.subject.materias | Ciencias Naturales | es |
sedici.subject.materias | Ciencias Agrarias | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Facultad de Ciencias Naturales y Museo | es |
mods.originInfo.place | Instituto de Fisiología Vegetal | es |
mods.originInfo.place | Facultad de Ciencias Agrarias y Forestales | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Journal of Experimental Botany | es |
sedici.relation.journalVolumeAndIssue | vol. 57, no. 8 | es |