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dc.date.accessioned 2019-10-10T13:40:25Z
dc.date.available 2019-10-10T13:40:25Z
dc.date.issued 2008
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/83021
dc.description.abstract The effect of the rhizobium adhesion protein RapA1 on Rhizobium leguminosarum bv. trifolii adsorption to Trifolium pratense (red clover) roots was investigated. We altered RapA1 production by cloning its encoding gene under the plac promoter into the stable vector pHC60. After introducing this plasmid in R. leguminosarum bv. trifolii, three to four times more RapA1 was produced, and two to five times higher adsorption to red clover roots was obtained, as compared with results for the empty vector. Enhanced adsorption was also observed on soybean and alfalfa roots, not related to R. leguminosarum cross inoculation groups. Although the presence of 1 mM Ca2+ during rhizobial growth enhanced adsorption, it was unrelated to RapA1 level. Similar effects were obtained when the same plasmid was introduced in Rhizobium etli for its adsorption to bean roots. Although root colonization by the RapA1-overproducing strain was also higher, nodulation was not enhanced. In addition, in vitro biofilm formation was similar to the wild-type both on polar and on hydrophobic surfaces. These results suggest that RapA1 receptors are present in root but not on inert surfaces, and that the function of this protein is related to rhizosphere colonization. en
dc.language en es
dc.subject Adhesin es
dc.subject Attachment es
dc.subject Biofilm es
dc.subject Nodulation es
dc.subject Rhizobium es
dc.title The rhizobial adhesion protein RapA1 is involved in adsorption of rhizobia to plant roots but not in nodulation en
dc.type Articulo es
sedici.identifier.other doi:10.1111/j.1574-6941.2008.00467.x es
sedici.identifier.other eid:2-s2.0-47249115323 es
sedici.identifier.issn 0168-6496 es
sedici.creator.person Mongiardini, Elías Javier es
sedici.creator.person Ausmees, Nora es
sedici.creator.person Pérez Giménez, Julieta es
sedici.creator.person Althabegoiti, María Julia es
sedici.creator.person Quelas, Juan Ignacio es
sedici.creator.person López García, Silvina Laura es
sedici.creator.person Lodeiro, Aníbal Roberto es
sedici.subject.materias Ciencias Exactas es
sedici.description.fulltext true es
mods.originInfo.place Facultad de Ciencias Exactas es
sedici.subtype Articulo es
sedici.rights.license Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
sedici.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
sedici.description.peerReview peer-review es
sedici.relation.journalTitle FEMS Microbiology Ecology es
sedici.relation.journalVolumeAndIssue vol. 65, no. 2 es


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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Excepto donde se diga explícitamente, este item se publica bajo la siguiente licencia Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)