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dc.date.accessioned | 2019-10-11T12:10:09Z | |
dc.date.available | 2019-10-11T12:10:09Z | |
dc.date.issued | 2005 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/83114 | |
dc.description.abstract | The G2 fusion subunit of the Junín virus envelope glycoprotein GP-C contains two hydrophobic heptad-repeat regions that are postulated to form a six-helix bundle structure required for the membrane fusion activity of Class I viral fusion proteins. We have investigated the role of these heptad-repeat regions and, specifically, the importance of the putative interhelical a and d position sidechains by using alanine-scanning mutagenesis. All the mutant glycoproteins were expressed and transported to the cell surface. Proteolytic maturation at the subtilisin kexin isozyme-1/site-1-protease (SKI-1/S1P) cleavage site was observed in all but two of the mutants. Among the adequately cleaved mutant glycoproteins, four positions in the N-terminal region (I333, L336, L347 and L350) and two positions in the C-terminal region (R392 and W395) were shown to be important determinants of cell-cell fusion. Taken together, our results indicate that α-helical coiled-coil structures are likely critical in promoting arenavirus membrane fusion. These findings support the inclusion of the arenavirus GP-C among the Class I viral fusion proteins and suggest pharmacologic and immunologic strategies for targeting arenavirus infection and hemorrhagic fever. | en |
dc.format.extent | 267-274 | es |
dc.language | en | es |
dc.subject | Arenavirus | es |
dc.subject | Envelope glycoprotein | es |
dc.subject | GP-C | es |
dc.subject | Junín virus or Argentine hemorrhagic fever virus | es |
dc.subject | Membrane fusion | es |
dc.subject | Mutagenesis | es |
dc.subject | Six-helix bundle | es |
dc.title | Genetic analysis of heptad-repeat regions in the G2 fusion subunit of the Junín arenavirus envelope glycoprotein | en |
dc.type | Articulo | es |
sedici.identifier.other | doi:10.1016/j.virol.2005.08.030 | es |
sedici.identifier.other | eid:2-s2.0-27744594720 | es |
sedici.identifier.issn | 0042-6822 | es |
sedici.creator.person | York, Joanne | es |
sedici.creator.person | Agnihothram, Sudhakar S. | es |
sedici.creator.person | Romanowski, Víctor | es |
sedici.creator.person | Nunberg, Jack H. | es |
sedici.subject.materias | Bioquímica | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Instituto de Biotecnologia y Biologia Molecular | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Virology | es |
sedici.relation.journalVolumeAndIssue | vol. 343, no. 2 | es |