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dc.date.accessioned 2011-11-10T13:21:05Z
dc.date.available 2011-11-10T03:00:00Z
dc.date.issued 2011 es
dc.identifier.uri http://hdl.handle.net/10915/8330
dc.description.abstract A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of tramadol in rat plasma using one-step protein precipitation was developed. After addition of ketamine as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on an SB-C18 (2.1 mm × 50 mm, 3.5 μm) column with methanol-0.1 % formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 264.0 for tramadol and m/z 237.8 for the IS. Calibration plots were linear over the range of 5-500 ng/mL for tramadol in rat plasma. Lower limit of quantification (LLOQ) for tramadol was 5 ng/mL. Mean recovery of tramadol from plasma was in the range 92.8 %-97.4 %. RSD of intra-day and inter-day precision were both less than 10 %. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of tramadol in rat plasma. es
dc.format.extent p. 1500-1505 es
dc.language es es
dc.title Gradient elution LC-ESI-MS determination of tramadol in rat plasma es
dc.type Articulo es
sedici.identifier.uri http://www.latamjpharm.org/resumenes/30/8/LAJOP_30_8_1_7.pdf es
sedici.creator.person Lin, Chongliang es
sedici.creator.person Lin, Guanyang es
sedici.creator.person Zhou, Ziye es
sedici.creator.person Hu, Lufeng es
sedici.creator.person Wang, Xianqin es
sedici.creator.person Ma Jianshe es
sedici.subject.materias Farmacia es
sedici.subject.decs Cromatografía Liquida es
sedici.subject.decs Espectrometría es
sedici.subject.decs Plasma es
sedici.subject.decs Tramadol es
sedici.subject.keyword gradient elution; LC-ESI-MS; rat plasma; tramadol es
sedici.description.fulltext false es
mods.originInfo.place Colegio de Farmacéuticos de la Provincia de Buenos Aires es
sedici.subtype Articulo es
sedici.description.peerReview peer-review es
sedici2003.identifier ARG-FARM-ART-0000001817 es
sedici.relation.journalTitle Latin American Journal of Pharmacy es
sedici.relation.journalVolumeAndIssue vol. 30, no. 8 es

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