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dc.date.accessioned 2011-12-22T13:30:06Z
dc.date.available 2011-12-22T03:00:00Z
dc.date.issued 2011 es
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/8373
dc.description.abstract A sensitive and selective liquid chromatography-mass spectrometry (LC-MS) method for determination of sulpiride in rabbit plasma was developed and validated. The analyte and internal standard (IS) were extracted from plasma by liquid-liquid extraction using ethyl acetate, and chromatography involved Agilent Extend-C18 column (2.1 mm x 50 mm, 3.5 μm) using 0.2 % formic acid in water and acetonitrile (60: 40, v/v) as a mobile phase. Detection involved positive ion mode electrospray ionization (ESI), and selective ion monitoring (SIM) mode was used for quantification of target fragmentions m/z 342.0 for sulpiride and m/z 294.8 for estazolam (internal standard, IS). The assay was linear over the range of 10-2000 ng/mL for sulpiride, with a lower limit of quantitation (LLOQ) of 10 ng/mL for sulpiride. Intraand inter-day precisions were less than 12 % and the accuracies were in the range of 94.1-108.7 % for sulpiride. This developed method was successfully applied for the determination of sulpiride in rabbit plasma for pharmacokinetic study. es
dc.format.extent 1775-1779 es
dc.language en es
dc.subject Sulpirida es
dc.subject Farmacocinética es
dc.subject Conejos es
dc.subject Plasma es
dc.subject Cromatografía Liquida es
dc.subject Espectrometría de Masas es
dc.title Determination of sulpiride in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study es
dc.type Articulo es
sedici.identifier.uri http://www.latamjpharm.org/resumenes/30/9/LAJOP_30_9_1_17.pdf es
sedici.creator.person Lin, Guanyang es
sedici.creator.person Wu, Haiya es
sedici.creator.person Wang, Zhibin es
sedici.creator.person Zhang, Haiyang es
sedici.creator.person Chien, Lianguo es
sedici.creator.person Wang, Xianqin es
sedici.creator.person Hu Lufeng es
sedici.creator.person Ma, Jianshe es
sedici.subject.materias Farmacia es
sedici.description.fulltext false es
mods.originInfo.place Colegio de Farmacéuticos de la Provincia de Buenos Aires es
sedici.subtype Articulo es
sedici.description.peerReview peer-review es
sedici2003.identifier ARG-FARM-ART-0000001861 es
sedici.relation.journalTitle Latin American Journal of Pharmacy es
sedici.relation.journalVolumeAndIssue vol. 30, no. 9 es


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