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dc.date.accessioned 2012-02-06T12:36:26Z
dc.date.available 2012-02-06T03:00:00Z
dc.date.issued 2011 es
dc.identifier.uri http://sedici.unlp.edu.ar/handle/10915/8418
dc.description.abstract A sensitive and selective liquid chromatography-mass spectrometry (LC-MS) method for determination of ibudilast in rabbit plasma was developed and validated. After addition of estazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation, and chromatography involved Agilent SB-C18 column (2.1 mm x 50 mm, 3.5 μm) using 0.1 % formic acid in water and acetonitrile as a mobile phase with gradient elution. Detection involved positive ion mode electrospray ionization (ESI), and selective ion monitoring (SIM) mode was used for quantification of target fragment ions m/z 230.7 for ibudilast and m/z 294.7 for estazolam (internal standard, IS). The assay was linear over the range of 20-2000 ng/mL for ibudilast, with a lower limit of quantitation (LLOQ) of 20 ng/mL for ibudilast. Intra- and inter-day precisions were less than 15 % and the accuracies were in the range of 90.78 %- 105.60 % for ibudilast. This developed method was successfully applied for the determination of ibudilast in rabbit plasma for pharmacokinetic study es
dc.format.extent 2065-2069 es
dc.language en es
dc.subject Agentes Antiinflamatorios es
dc.subject Espectrometría de Masas es
dc.subject Plasma es
dc.subject Farmacocinética es
dc.title Determination of ibudilast in rabbit plasma by liquid chromatography-mass spectrometry and its application es
dc.type Articulo es
sedici.identifier.uri http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_2_3.pdf es
sedici.creator.person Hu, Lufeng es
sedici.creator.person Chen, Xiaole es
sedici.creator.person Ma, Jianshe es
sedici.creator.person Wang, Xianqin es
sedici.creator.person Sun, Wei es
sedici.creator.person Dong, Wei es
sedici.creator.person Yang, Fangfang es
sedici.creator.person Ding, Chen es
sedici.subject.materias Farmacia es
sedici.description.fulltext false es
mods.originInfo.place Colegio de Farmacéuticos de la Provincia de Buenos Aires es
sedici.subtype Comunicacion es
sedici.description.peerReview peer-review es
sedici2003.identifier ARG-FARM-ART-0000001906 es
sedici.relation.journalTitle Latin American Journal of Pharmacy es
sedici.relation.journalVolumeAndIssue vol. 30, no. 10 es


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