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dc.date.accessioned | 2019-10-28T14:59:47Z | |
dc.date.available | 2019-10-28T14:59:47Z | |
dc.date.issued | 2011 | |
dc.identifier.uri | http://sedici.unlp.edu.ar/handle/10915/84201 | |
dc.description.abstract | The use of antagonists of the mineralocorticoid receptor in the treatment of myocardial hypertrophy and heart failure has gained increasing importance in the last years. The cardiac Na/H exchanger (NHE-1) upregulation induced by aldosterone could account for the genesis of these pathologies. We tested whether aldosterone-induced NHE-1 stimulation involves the transactivation of the epidermal growth factor receptor (EGFR). Rat ventricular myocytes were used to measure intracellular pH with epifluorescence. Aldosterone enhanced the NHE-1 activity. This effect was canceled by spironolactone or eplerenone (mineralocorticoid receptor antagonists), but not by mifepristone (glucocorticoid receptor antagonist) or cycloheximide (protein synthesis inhibitor), indicating that the mechanism is mediated by the mineralocorticoid receptor triggering nongenomic pathways. Aldosterone-induced NHE-1 stimulation was abolished by the EGFR kinase inhibitor AG1478, suggesting that is mediated by transactivation of EGFR. The increase in the phosphorylation level of the kinase p90 RSK and NHE-1 serine703 induced by aldosterone was also blocked by AG1478. Exogenous epidermal growth factor mimicked the effects of aldosterone on NHE-1 activity. Epidermal growth factor was also able to increase reactive oxygen species production, and the epidermal growth factor-induced activation of the NHE-1 was abrogated by the reactive oxygen species scavenger N-2-mercaptopropionyl glycine, indicating that reactive oxygen species are participating as signaling molecules in this mechanism. Aldosterone enhances the NHE-1 activity via transactivation of the EGFR, formation of reactive oxygen species, and phosphorylation of the exchanger. These results call attention to the consideration of the EGFR as a new potential therapeutic target of the cardiovascular pathologies involving the participation of aldosterone. | en |
dc.format.extent | 912-919 | es |
dc.language | en | es |
dc.subject | Aldosterone | es |
dc.subject | Cardiac myocytes | es |
dc.subject | Epidermal growth factor receptor | es |
dc.subject | Sodium/hydrogen exchanger | es |
dc.subject | Transactivation | es |
dc.title | Aldosterone stimulates the cardiac Na +/H + exchanger via transactivation of the epidermal growth factor receptor | en |
dc.type | Articulo | es |
sedici.identifier.other | doi:10.1161/HYPERTENSIONAHA.111.176024 | es |
sedici.identifier.other | eid:2-s2.0-83155183297 | es |
sedici.identifier.issn | 0194-911X | es |
sedici.creator.person | De Giusti, Verónica Celeste | es |
sedici.creator.person | Nolly, Mariela | es |
sedici.creator.person | Yeves, Alejandra M. | es |
sedici.creator.person | Caldiz, Claudia Irma | es |
sedici.creator.person | Villa Abrille, María Celeste | es |
sedici.creator.person | Chiappe de Cingolani, Gladys Ethel | es |
sedici.creator.person | Ennis, Irene Lucía | es |
sedici.creator.person | Cingolani, Horacio Eugenio | es |
sedici.creator.person | Aiello, Ernesto Alejandro | es |
sedici.subject.materias | Ciencias Médicas | es |
sedici.description.fulltext | true | es |
mods.originInfo.place | Facultad de Ciencias Médicas | es |
sedici.subtype | Articulo | es |
sedici.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
sedici.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
sedici.description.peerReview | peer-review | es |
sedici.relation.journalTitle | Hypertension | es |
sedici.relation.journalVolumeAndIssue | vol. 58, no. 5 | es |
sedici.rights.sherpa | * Color: amarillo* Pre-print del autor: si* Post-print del autor: restricted* Versión de editor/PDF:no* Condiciones:>>Author's pre-print on pre-print server only>>Must inform publisher of any pre-print deposit>>Author's pre-print must not be updated with future versions>>Author's post-print on Institutional repository or funding agency repository>>En un servidor sin ánimo de lucro>>La versión de editor/PDF no puede utilizarse>>Publisher will automatically deposit authors post-print in PubMed Central for NIH funded authors after 12 months>>Publisher will automatically deposit authors post-print in PubMed Central for HHMI and Wellcome Trust funded authors after 6 months>>Authors may place a 'toll-free' link to their article on authors' personal website or institutional website without embargo>>Debe enlazar a la versión de editor con DOI>>Publisher last contacted on 07/06/2018* Link a Sherpa: http://sherpa.ac.uk/romeo/issn/0194-911X/es/ |